Standard name
Human Ortholog
Description Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0.06 0.12 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.67 0.83 0.85 0.89 0.7 0.51 0.42 0.3 0.26 0.22 0.17 0.85 0.8 0.76 0.16 0.26 0.22 0.25 0.46 0.2 0.61 0.63 0.55
Endoplasmic Reticulum 0 0.23 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.11 0 0.1 0.05 0.32 0.2 0.3 0.12 0.1 0.1
Endosome 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.51 0.41 0.5 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.12 0 0.13 0.12 0.37 0.57 0.61 0.74 0.78 0.85 0.88 0 0 0 0.08 0.11 0.16 0.24 0.14 0.19 0.12 0.09 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.2 0.15 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.06 0.06 0.08 0.07 0.08 0 0 0 0.12 0.09 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 2 1 0 0 0 0 0 0 0 0 4 12 1 4 2 6 10 10 40 5 11 23
Bud 5 2 3 12 6 13 23 18 27 31 20 20 1 1 0 1 2 2 3 0 4 2 5 10
Bud Neck 0 1 3 0 0 1 1 0 1 0 0 0 5 7 2 0 2 0 5 3 6 6 5 13
Bud Site 1 0 0 1 0 0 1 3 5 16 2 8 0 0 0 0 0 0
Cell Periphery 1 1 4 4 3 2 2 1 0 1 0 0 0 4 3 0 1 0 3 0 5 9 12 17
Cytoplasm 158 101 226 236 376 299 333 272 162 166 84 80 247 321 288 8 21 24 62 81 65 198 273 294
Endoplasmic Reticulum 1 34 4 1 1 7 3 2 3 1 1 6 32 27 40 1 8 6 79 35 101 38 42 50
Endosome 3 1 20 10 11 16 23 19 14 6 2 4 5 19 28 25 34 55 5 6 14 3 10 18
Golgi 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 1 1 3 1 1 6 0 1 0
Mitochondria 51 18 7 36 52 159 369 397 402 500 318 413 1 1 0 4 9 18 60 24 64 38 40 62
Nucleus 1 0 0 0 2 4 4 4 2 4 5 8 3 0 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 3 3 2 1 1 2 5 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 3 0 0 0 0 1 4 2 1 2 3 3 0 0 0 0 1 2 0 0 0 0 0 0
Peroxisomes 0 0 9 11 4 2 1 0 3 3 0 3 0 4 0 0 0 0 0 1 4 0 1 0
SpindlePole 0 1 10 7 4 5 8 21 6 16 5 11 4 13 29 10 12 17 2 2 2 3 5 7
Vac/Vac Membrane 4 0 6 4 4 12 30 40 30 51 28 37 1 11 5 6 7 11 10 6 9 12 14 22
Unique Cell Count 203 150 271 279 424 428 649 647 540 641 374 471 290 401 380 49 82 110 251 179 335 328 431 533
Labelled Cell Count 228 165 295 323 463 525 805 781 657 798 470 598 303 421 398 61 100 144 251 179 335 328 431 533


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.6 4.8 4.4 4.3 3.9 3.6 3.9 3.6 3.6 3.4 3.5 5.7 5.7 5.6 6.9 6.5 6.4 5.8 5.9 6.2
Std Deviation (1e-4) 0.6 0.7 1.4 2.0 1.6 1.7 1.4 1.6 1.2 0.9 1.3 1.3 0.9 1.1 1.0 1.4 1.3 1.2 1.1 1.2 1.3
Intensity Change (Log2) -0.11 -0.15 -0.31 -0.4 -0.3 -0.42 -0.43 -0.51 -0.45 0.25 0.26 0.22 0.52 0.44 0.42 0.29 0.3 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3478 2.7268 2.4717 2.5252 1.9419 2.7515 -0.1352 0.0177 -0.0088 -0.6801 -0.83 -0.3719 -1.2679 -0.8568 -1.1226 -1.1347 -0.7471 -1.15
Actin 0.0171 0.0027 0.0143 0.0012 0.0042 0.003 0.05 0.0026 0.0065 0.0012 0.0136 0.0122 0.0352 0.0005 0.0063 0.0057 0.0529 0.0059
Bud 0.0003 0.0003 0.0019 0.0004 0.0004 0.0001 0.0068 0.0028 0.001 0.0001 0.0006 0.0008 0.0011 0.0003 0.0002 0.0004 0.0005 0.0002
Bud Neck 0.002 0.0003 0.0092 0.0007 0.0003 0.0011 0.0066 0.0006 0.0048 0.0004 0.004 0.0026 0.0075 0.0004 0.0031 0.0056 0.0032 0.0084
Bud Periphery 0.0012 0.0006 0.0048 0.001 0.0009 0.0003 0.0056 0.0013 0.0009 0.0001 0.0016 0.0017 0.0012 0.0004 0.0002 0.0006 0.0011 0.0003
Bud Site 0.0044 0.0035 0.0086 0.0016 0.0006 0.0005 0.0124 0.0162 0.0079 0.0003 0.0008 0.0018 0.0089 0.0038 0.0015 0.0025 0.0014 0.0002
Cell Periphery 0.0034 0.0018 0.0048 0.0009 0.0004 0.0007 0.0016 0.001 0.0005 0.0002 0.0002 0.0003 0.0011 0.0005 0.0007 0.0002 0.0004 0.0009
Cytoplasm 0.1263 0.2195 0.2232 0.1758 0.1673 0.2037 0.1661 0.4049 0.3729 0.4613 0.379 0.4669 0.1644 0.2275 0.2915 0.3423 0.1949 0.283
Cytoplasmic Foci 0.0545 0.0415 0.0447 0.0738 0.0324 0.0412 0.0693 0.069 0.0623 0.0346 0.0907 0.0541 0.1181 0.0793 0.0702 0.0973 0.1205 0.0662
Eisosomes 0.0041 0.0007 0.001 0.0004 0.0003 0.0003 0.0005 0.0002 0.0001 0.0001 0.0003 0.0002 0.0007 0.0003 0.0003 0.0002 0.0004 0.0001
Endoplasmic Reticulum 0.0094 0.0096 0.0153 0.005 0.0032 0.0083 0.0149 0.0287 0.0203 0.0164 0.0081 0.0139 0.0127 0.0132 0.0183 0.0211 0.0095 0.0131
Endosome 0.0251 0.0479 0.0325 0.0256 0.0128 0.0218 0.0559 0.055 0.0572 0.039 0.099 0.0336 0.0455 0.064 0.0533 0.0177 0.0905 0.0337
Golgi 0.0044 0.0033 0.0032 0.0031 0.0028 0.0017 0.0157 0.0027 0.0086 0.0027 0.009 0.0027 0.007 0.0029 0.0034 0.0031 0.0222 0.003
Lipid Particles 0.0278 0.0308 0.0267 0.0149 0.0104 0.0217 0.0337 0.0228 0.0196 0.017 0.0259 0.0161 0.0274 0.0294 0.0135 0.0123 0.028 0.0157
Mitochondria 0.0064 0.0016 0.0022 0.0009 0.0074 0.0007 0.0196 0.001 0.0058 0.0032 0.0367 0.0021 0.0097 0.0003 0.0008 0.0017 0.0041 0.0081
None 0.6885 0.6227 0.5903 0.679 0.7318 0.6775 0.491 0.3697 0.3936 0.3756 0.3156 0.3744 0.5263 0.5654 0.5151 0.4665 0.4464 0.5472
Nuclear Periphery 0.0035 0.0019 0.0027 0.0016 0.0006 0.0015 0.0052 0.0043 0.006 0.0081 0.0027 0.0025 0.0058 0.0017 0.0037 0.0032 0.0049 0.0023
Nucleolus 0.002 0.0009 0.0009 0.0006 0.0002 0.0004 0.0026 0.0013 0.0013 0.0002 0.0002 0.0005 0.0011 0.0003 0.0004 0.0004 0.0006 0.0003
Nucleus 0.0024 0.0012 0.0015 0.0011 0.0008 0.001 0.0026 0.0024 0.0051 0.0019 0.0011 0.003 0.0034 0.001 0.0016 0.0014 0.0018 0.0012
Peroxisomes 0.0043 0.0009 0.0037 0.0041 0.0202 0.0053 0.0171 0.0015 0.0136 0.0258 0.0018 0.0021 0.0076 0.0012 0.0057 0.0133 0.0035 0.0023
Punctate Nuclear 0.0039 0.003 0.0028 0.0045 0.0011 0.0065 0.0053 0.0034 0.0056 0.0026 0.0047 0.0047 0.0099 0.0026 0.0047 0.0028 0.007 0.004
Vacuole 0.0081 0.0048 0.0054 0.0035 0.0016 0.0025 0.0129 0.0082 0.005 0.0067 0.0029 0.0031 0.0047 0.0046 0.005 0.0016 0.0051 0.0034
Vacuole Periphery 0.0008 0.0005 0.0006 0.0003 0.0002 0.0002 0.0046 0.0005 0.0012 0.0025 0.0016 0.0006 0.0008 0.0003 0.0005 0.0003 0.0011 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1925 4.6418 4.0185 8.4359 5.0492 7.7844 5.4755 7.1272 9.3698 9.524
Translational Efficiency 1.4771 1.3564 1.6893 0.7607 0.9935 1.0384 0.9038 0.8388 0.7188 0.6598

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency