Standard name
Human Ortholog
Description Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; required for ribosomal small subunit export from nucleus; required for growth at low temperature

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.99 0.97 0.96 0.82 0.77 0.73 0.65 0.68 0.67 0.98 0.99 0.99 0.95 0.91 0.96 0.96 0.96 0.88 0.94 0.95 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.29 0.35 0.33 0.46 0.46 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 1 0 1 0 1 3 7 2 1 1 4 1 3 2 0 0 1 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 9 2 0 0 1 3 2 10 6 3 1 9 12 15 15 14 17 9 0 0 0 0 0 0
Cytoplasm 334 212 70 97 112 149 137 208 108 71 95 113 475 610 755 368 244 215 338 202 81 289 441 428
Endoplasmic Reticulum 2 4 1 0 0 1 1 5 3 4 7 8 9 2 4 9 6 4 2 1 3 8 11 26
Endosome 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 1 0 1 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 3 1 0 0 0 2 3
Mitochondria 7 0 0 1 2 12 48 93 49 50 65 78 2 1 3 6 1 2 1 0 1 1 1 2
Nucleus 0 0 0 0 0 1 0 2 1 1 2 0 3 0 0 1 1 2 0 3 0 0 0 0
Nuclear Periphery 0 0 1 1 0 0 2 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 1 0 1 0 1 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 3 1 1 0 0 0 3 3 0 4 0 1 0 0 2 10 14 3 0 1 0 0 1 6
Unique Cell Count 344 217 73 98 115 156 168 269 147 109 140 168 484 614 765 387 268 225 352 212 92 306 465 481
Labelled Cell Count 358 220 73 101 116 167 199 330 170 134 171 215 502 631 781 410 292 240 352 212 92 306 465 481


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 11.5 8.3 8.4 7.1 6.2 5.1 5.3 5.4 4.8 5.1 4.7 11.3 10.6 10.3 17.3 21.0 20.6 12.4 11.3 11.7
Std Deviation (1e-4) 1.7 1.9 1.5 1.1 1.0 1.0 1.3 1.0 1.0 0.7 0.9 0.8 1.5 1.6 1.5 4.0 4.3 4.8 1.7 1.7 1.8
Intensity Change (Log2) 0.01 -0.22 -0.44 -0.7 -0.64 -0.63 -0.79 -0.71 -0.82 0.44 0.35 0.3 1.05 1.34 1.31 0.57 0.44 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 0.6 -0.1 -2.9 -3.6 -4.0 -4.9 -4.6 -4.8 1.2 2.8 1.9 -0.3 -1.4 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.1 5.9 5.6 0 7.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6513 11.3687 10.4116 9.3557 8.7775 10.4777 5.9403 8.917 7.4457 7.395 7.3808 7.9228 9.3387 10.5126 10.7208 10.7577 9.8002 10.5906
Actin 0.0613 0.0004 0.0303 0.0697 0.0972 0.0112 0.0228 0.0115 0.0082 0.0519 0.0492 0.0038 0.0323 0.0013 0.0085 0.031 0.0282 0.0004
Bud 0.001 0.0002 0.0015 0.0027 0.0005 0.0003 0.0005 0.0003 0.0004 0.0004 0.0008 0.0002 0.0008 0.0043 0.0003 0.0009 0.0007 0.0001
Bud Neck 0.0059 0.0002 0.0008 0.0025 0.0037 0.0014 0.0016 0.0003 0.0003 0.0054 0.0003 0.0075 0.0053 0.0002 0.0006 0.0024 0.0007 0.0007
Bud Periphery 0.0013 0.0001 0.0006 0.0047 0.0006 0.0003 0.0005 0.0001 0.0002 0.0002 0.0013 0.0001 0.0009 0.0016 0.0001 0.0004 0.0004 0
Bud Site 0.0016 0.0007 0.0012 0.0024 0.0018 0.0003 0.0024 0.0051 0.0009 0.001 0.0007 0.0001 0.003 0.0021 0.0009 0.0015 0.0008 0
Cell Periphery 0.0005 0.0002 0.0002 0.0004 0.0002 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0005 0.0004 0.0001 0.0001 0.0001 0
Cytoplasm 0.6856 0.9653 0.8806 0.7443 0.7489 0.8942 0.7325 0.9609 0.9058 0.82 0.8256 0.9424 0.7705 0.9512 0.9363 0.84 0.8516 0.9574
Cytoplasmic Foci 0.0199 0.0036 0.0187 0.0178 0.0109 0.0099 0.0423 0.0067 0.0136 0.0214 0.0173 0.0126 0.0266 0.005 0.0092 0.0224 0.0158 0.0061
Eisosomes 0.001 0 0.0001 0.0003 0.0013 0 0.0003 0.0001 0 0.0001 0.0002 0 0.0003 0.0002 0 0 0.0002 0
Endoplasmic Reticulum 0.0394 0.0139 0.0113 0.0121 0.0202 0.0297 0.0396 0.0017 0.0069 0.003 0.0032 0.0114 0.0502 0.0064 0.0125 0.0132 0.0083 0.0165
Endosome 0.0394 0.0074 0.0161 0.0633 0.0391 0.0225 0.0581 0.0067 0.0369 0.0474 0.0319 0.0146 0.0291 0.007 0.0138 0.0394 0.0455 0.0125
Golgi 0.0111 0.0009 0.0036 0.0206 0.0101 0.0021 0.0195 0.001 0.0043 0.012 0.0035 0.0026 0.0103 0.0012 0.0047 0.0027 0.0089 0.001
Lipid Particles 0.0181 0.0001 0.0026 0.0029 0.0102 0.0002 0.0068 0.0002 0.0004 0.0047 0.0013 0.0001 0.0067 0.0014 0.002 0.0006 0.0047 0
Mitochondria 0.0054 0.0006 0.002 0.011 0.0121 0.0017 0.0177 0.0007 0.0017 0.0046 0.0013 0.0011 0.0037 0.0047 0.0007 0.0012 0.003 0.0006
None 0.0105 0.0007 0.0088 0.0016 0.0027 0.0013 0.0209 0.0014 0.0127 0.0009 0.0016 0.0006 0.0242 0.007 0.001 0.001 0.0029 0.0012
Nuclear Periphery 0.0184 0.0012 0.0046 0.0047 0.0164 0.0122 0.0071 0.0002 0.0009 0.0008 0.0529 0.0004 0.0059 0.0011 0.0015 0.0028 0.0154 0.0008
Nucleolus 0.0009 0 0.0003 0.0001 0.0006 0.0001 0.0005 0 0 0.0001 0.0014 0 0.0008 0.0005 0 0.0031 0.0002 0
Nucleus 0.0239 0.0011 0.0067 0.0038 0.0037 0.0071 0.0037 0.0004 0.0006 0.0007 0.0018 0.0004 0.0056 0.0012 0.0009 0.0039 0.0033 0.0009
Peroxisomes 0.0218 0 0.0014 0.0077 0.0058 0.0002 0.0074 0.0006 0.0002 0.0162 0.0004 0.0005 0.0085 0.0009 0.0034 0.0016 0.0016 0
Punctate Nuclear 0.0219 0.0001 0.0029 0.0008 0.0066 0.0013 0.0082 0.0001 0.0002 0.0002 0.0018 0.0001 0.0075 0.0003 0.0006 0.0029 0.003 0.0001
Vacuole 0.0089 0.0028 0.0048 0.0201 0.005 0.0027 0.0057 0.0017 0.0046 0.0068 0.0017 0.001 0.0059 0.0018 0.0023 0.0261 0.0032 0.0013
Vacuole Periphery 0.0022 0.0005 0.0011 0.0063 0.0022 0.0011 0.0013 0.0003 0.0009 0.002 0.0017 0.0005 0.0014 0.0003 0.0005 0.0028 0.0016 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.4814 40.3904 34.7038 26.8836 51.3869 28.5205 29.1961 22.9695 22.5915 32.4498
Translational Efficiency 3.5714 2.4834 2.4589 2.7623 1.5669 2.213 1.8631 1.8227 1.5276 1.9368

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1526 376 112 1075 1339 1728 224 138 2865 2104 336 1213

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1015.94 1111.54 1311.11 1499.56 1042.66 1150.54 1530.57 1569.36 1028.43 1143.57 1457.42 1507.50
Standard Deviation 140.47 221.14 145.58 214.44 147.17 179.28 199.55 206.68 144.26 188.04 210.51 214.72
Intensity Change Log 2 0.129745 0.367974 0.561724 0.142042 0.553800 0.589908 0.135986 0.465081 0.576068

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000248 0.000385 0.000798 0.000582 0.000247 0.000267 0.001129 0.000466 0.000247 0.000288 0.001018 0.000569
Bud Neck 0.000776 0.000968 0.002624 0.001778 0.000575 0.000670 0.002180 0.002703 0.000682 0.000723 0.002328 0.001883
Bud Site 0.012330 0.034442 0.023355 0.020442 0.015485 0.022327 0.030382 0.033449 0.013804 0.024492 0.028040 0.021922
Cell Periphery 0.000149 0.000266 0.000329 0.000661 0.000184 0.000163 0.000343 0.000523 0.000165 0.000182 0.000338 0.000645
Cytoplasm 0.791934* 0.789725* 0.471742* 0.895298* 0.818037* 0.852337* 0.740730* 0.739964* 0.804134* 0.841148* 0.651067* 0.877626*
Cytoplasmic Foci 0.135438 0.108696 0.136628 0.020556 0.117161 0.088821 0.085613 0.060855 0.126896 0.092373 0.102618 0.025141
Eisosomes 0.000010 0.000007 0.000029 0.000005 0.000008 0.000009 0.000016 0.000008 0.000009 0.000008 0.000020 0.000005
Endoplasmic Reticulum 0.004031 0.002182 0.004121 0.000220 0.005153 0.001239 0.000635 0.000122 0.004556 0.001407 0.001797 0.000209
Endosome 0.006764 0.009064 0.030083 0.002760 0.005093 0.003815 0.008186 0.004840 0.005983 0.004753 0.015485 0.002996
Golgi 0.007379 0.008628 0.002339 0.004809 0.007967 0.010124 0.001627 0.001403 0.007654 0.009857 0.001865 0.004422
Lipid Particles 0.000889 0.000943 0.005298 0.004516 0.000984 0.000481 0.004820 0.005136 0.000933 0.000564 0.004979 0.004587
Mitochondria 0.000589 0.000642 0.000046 0.000138 0.000446 0.000340 0.000009 0.000031 0.000522 0.000394 0.000022 0.000126
Mitotic Spindle 0.000563 0.000923 0.002525 0.002185 0.000464 0.000503 0.002967 0.001381 0.000516 0.000578 0.002820 0.002093
None 0.001261 0.000637 0.000107 0.000273 0.001077 0.000523 0.000350 0.000129 0.001175 0.000543 0.000269 0.000257
Nuclear Periphery 0.000025 0.000034 0.000285 0.000047 0.000037 0.000010 0.000086 0.000028 0.000030 0.000015 0.000152 0.000045
Nuclear Periphery Foci 0.000629 0.001365 0.011698 0.002107 0.000777 0.000230 0.007485 0.003033 0.000698 0.000433 0.008889 0.002213
Nucleolus 0.000104 0.000374 0.000086 0.000128 0.000084 0.000085 0.000038 0.000114 0.000095 0.000137 0.000054 0.000127
Nucleus 0.005826 0.003848 0.009336 0.005221 0.005040 0.002450 0.008426 0.003509 0.005458 0.002700 0.008729 0.005026
Peroxisomes 0.000355 0.000940 0.000823 0.000476 0.000252 0.000583 0.000519 0.001653 0.000307 0.000647 0.000621 0.000610
Vacuole 0.025240 0.030721 0.296130* 0.036670 0.014904 0.011969 0.104057 0.140163 0.020410 0.015320 0.168081 0.048444
Vacuole Periphery 0.005462 0.005213 0.001618 0.001128 0.006025 0.003054 0.000402 0.000491 0.005725 0.003440 0.000807 0.001055

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.14 -2.57 -2.95 -3.09 0.76 -0.61 -5.91 -3.61 -3.02 4.16 -0.63 -5.74 -4.45 -5.54 2.83
Bud Neck -1.38 -5.09 -3.00 -2.42 1.15 -0.58 -8.36 -7.02 -6.45 -1.03 -0.34 -9.66 -3.99 -3.72 1.35
Bud Site -7.00 -3.79 -5.27 4.12 0.89 -4.12 -2.91 -3.13 -1.77 -0.64 -7.75 -4.25 -5.39 1.45 1.36
Cell Periphery -2.20 -3.16 -10.72 -5.19 -4.53 1.18 -3.73 -4.13 -4.48 -1.87 -1.11 -5.20 -10.96 -10.23 -5.79
Cytoplasm 0.23 12.01 -11.61 -6.97 -15.11 -4.77 7.50 5.84 7.46 -0.16 -6.32 12.14 -7.87 -2.14 -14.97
Cytoplasmic Foci 3.48 0.44 27.39 12.74 10.52 5.60 4.52 6.55 3.29 2.61 8.56 4.39 32.11 20.24 13.90
Eisosomes 2.80 -6.44 6.22 1.99 8.18 -0.31 -4.54 -1.09 -0.19 3.48 0.33 -7.18 5.79 1.35 9.42
Endoplasmic Reticulum 1.87 0.30 5.93 2.67 4.04 4.76 6.15 6.74 3.28 6.10 5.42 5.15 8.88 3.88 4.59
Endosome -1.74 -6.19 5.16 5.02 7.44 2.25 -2.54 0.45 -0.94 2.36 2.24 -5.90 5.19 3.09 7.96
Golgi -1.36 8.51 2.89 3.26 -1.70 -2.22 20.70 14.24 9.01 0.48 -2.73 20.47 3.95 4.94 -2.27
Lipid Particles -0.25 -4.43 -8.42 -7.81 -0.36 3.69 -6.96 -5.09 -5.64 -0.33 4.08 -8.33 -9.29 -10.10 -0.08
Mitochondria -0.08 1.67 1.41 0.92 -1.36 1.06 5.26 5.00 5.39 -1.07 0.61 2.82 2.23 2.34 -1.81
Mitotic Spindle -1.40 -1.65 -2.02 -1.61 0.22 -0.21 -3.78 -1.10 -1.08 -0.28 -0.36 -3.97 -2.36 -2.28 0.62
None 2.91 8.91 7.15 2.31 -4.25 2.34 3.14 4.07 5.18 2.54 4.47 6.84 6.80 3.80 -0.42
Nuclear Periphery -0.68 -3.71 -1.44 -0.93 3.07 3.67 -7.39 -1.63 -4.74 5.85 3.07 -6.21 -1.32 -2.02 3.98
Nuclear Periphery Foci -2.13 -4.33 -8.29 -3.02 3.52 6.44 -10.02 -5.90 -7.09 5.55 3.22 -9.04 -9.23 -10.11 6.82
Nucleolus -0.95 0.78 -0.87 0.89 -1.52 -0.06 1.84 -1.02 -0.45 -3.89 -0.85 2.19 -1.22 0.47 -2.43
Nucleus 1.94 -2.91 -2.54 -3.23 1.83 6.10 -5.96 0.84 -2.97 5.63 8.07 -6.32 -3.04 -8.38 3.80
Peroxisomes -1.86 -4.56 -2.66 1.47 3.22 -2.58 -4.70 -4.29 -2.82 -3.22 -2.85 -6.00 -5.76 0.49 0.48
Vacuole -1.21 -10.79 -8.09 -3.88 9.83 2.03 -11.42 -9.43 -9.61 -1.27 3.63 -14.71 -12.89 -14.37 10.88
Vacuole Periphery 0.22 6.02 6.81 4.31 1.41 4.04 8.39 8.26 9.08 -1.34 4.22 10.77 9.66 7.17 -0.98