ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.98 0.97 0.92 0.9 0.84 0.8 0.79 0.74 0.76 0.99 0.99 0.99 0.98 0.94 0.94 0.96 0.95 0.97 0.96 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.13 0.14 0.3 0.28 0.34 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0 0.07 0 0 0 0.07 0 0 0 0.08 0.23 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 1 0 2 1 0 2 4 4 1 0 1 8 1 2 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 2 0 2 2 3 1 2 8 3 3 3 2 2 6 2 0 0 0 0 0 0 0 0
Cytoplasm 454 325 201 228 255 380 388 506 413 348 291 360 288 477 536 47 33 68 452 303 194 192 377 392
Endoplasmic Reticulum 1 1 0 1 0 0 0 2 3 2 7 7 1 3 2 0 0 1 0 0 1 0 3 4
Endosome 0 0 0 0 0 0 0 2 0 1 0 1 0 1 1 0 0 0 4 2 0 1 2 1
Golgi 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 5
Mitochondria 3 4 7 3 3 29 54 86 154 124 134 155 0 0 2 1 0 0 2 2 0 0 0 0
Nucleus 2 2 1 3 2 1 3 8 8 4 5 7 3 3 1 1 1 2 1 0 0 2 1 3
Nuclear Periphery 0 0 0 1 1 0 2 2 1 1 1 2 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 2 1 0 6 20 17 41 10 13 3 32 0 1 2 4 8 9 2 5 1 1 4 3
Unique Cell Count 464 329 206 232 263 412 429 601 519 438 394 472 290 482 544 48 35 72 472 321 201 202 398 418
Labelled Cell Count 469 336 213 239 269 435 470 654 598 496 445 575 295 489 551 55 42 81 472 321 201 202 398 418


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 9.5 5.9 8.3 8.5 7.2 6.1 5.9 4.9 5.2 4.8 4.7 11.2 10.6 10.3 12.5 12.4 11.9 10.3 11.2 11.5
Std Deviation (1e-4) 1.1 1.3 1.1 1.4 1.5 1.2 1.4 1.2 1.0 1.4 0.9 1.0 1.6 1.5 1.3 2.1 2.0 1.7 1.7 2.0 2.0
Intensity Change (Log2) 0.5 0.54 0.29 0.07 0.0 -0.24 -0.18 -0.28 -0.32 0.93 0.86 0.81 1.09 1.08 1.03 0.82 0.94 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.4 -2.6 -3.2 -5.0 -6.1 -6.0 -7.2 -6.8 1.6 1.4 0.9 0.1 -1.1 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.8 3.7 4.2 7.7 7.3 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.8 2.5 3.5 0 0 0 3.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5307 11.2822 10.0063 9.2971 9.1829 9.9924 6.9481 9.6378 8.2774 7.9756 9.1124 8.4714 11.0916 13.3436 12.3609 11.557 11.8114 11.6269
Actin 0.0592 0.0003 0.0303 0.0003 0.0647 0.0059 0.0519 0.0002 0.0436 0.0227 0.0001 0.0127 0.0056 0.0002 0.0007 0.008 0.0001 0.0011
Bud 0.0012 0.0001 0.0005 0.0001 0.0002 0.0003 0.0008 0 0.0004 0.0008 0 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003
Bud Neck 0.0023 0.0002 0.0013 0.0005 0.0006 0.0022 0.0021 0.0001 0.0007 0.0012 0.0003 0.0088 0.0005 0.0001 0.0004 0.0014 0.0006 0.0017
Bud Periphery 0.0012 0 0.0008 0 0.0003 0.0001 0.0007 0 0.0005 0.0007 0 0.0003 0.0001 0 0 0 0 0.0001
Bud Site 0.0038 0.0002 0.0015 0.0001 0.0004 0.0005 0.0044 0.0001 0.0024 0.0032 0 0.0002 0.0002 0.0004 0.0002 0.0003 0 0.0001
Cell Periphery 0.0004 0 0.0002 0 0 0 0.0003 0 0.0001 0.0001 0 0.0001 0 0 0 0 0 0
Cytoplasm 0.824 0.9885 0.9089 0.9585 0.89 0.9323 0.7172 0.9913 0.8553 0.8699 0.9876 0.903 0.9318 0.9872 0.9732 0.9585 0.9796 0.9426
Cytoplasmic Foci 0.0159 0.0005 0.0072 0.0131 0.0018 0.0067 0.0425 0.0007 0.0258 0.0368 0.0009 0.0114 0.0103 0.0006 0.0061 0.0061 0.0008 0.0054
Eisosomes 0.0003 0 0.0002 0 0.0002 0 0.0002 0 0.0005 0.0001 0 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0124 0.0005 0.0032 0.0018 0.0015 0.0024 0.0086 0.0005 0.002 0.0008 0.0008 0.0034 0.0044 0.0006 0.0009 0.0014 0.0006 0.0031
Endosome 0.0248 0.0001 0.0081 0.0032 0.0019 0.0078 0.076 0.0001 0.0157 0.0103 0.0003 0.0122 0.0086 0.0001 0.0009 0.0016 0.0002 0.005
Golgi 0.0064 0 0.0032 0.0003 0.0015 0.0015 0.0213 0 0.0153 0.0165 0 0.0133 0.0008 0 0.0002 0.0004 0 0.0004
Lipid Particles 0.0048 0 0.0027 0.0027 0.0004 0.0011 0.0106 0 0.004 0.003 0 0.0015 0.0012 0 0.0006 0.0001 0 0.0002
Mitochondria 0.0062 0.0001 0.0079 0.0001 0.0012 0.0011 0.0038 0 0.0094 0.0036 0 0.0067 0.0004 0.0001 0.0001 0.0002 0.0001 0.0005
None 0.0057 0.001 0.0019 0.0057 0.001 0.0018 0.0112 0.0013 0.0038 0.0012 0.0006 0.0009 0.0109 0.0008 0.0016 0.003 0.0005 0.0036
Nuclear Periphery 0.0087 0.0008 0.0032 0.0018 0.0024 0.0122 0.0116 0.0007 0.0014 0.0017 0.0008 0.0015 0.0045 0.0008 0.0012 0.0013 0.0014 0.0057
Nucleolus 0.0002 0 0.0002 0.0001 0 0.0002 0.0004 0 0.0001 0 0 0 0.0001 0 0 0 0 0.0002
Nucleus 0.0121 0.0075 0.0077 0.0081 0.0298 0.0177 0.0145 0.0045 0.0073 0.0111 0.008 0.0159 0.0142 0.0085 0.0124 0.0152 0.0153 0.0251
Peroxisomes 0.0033 0 0.008 0.0001 0.0004 0.0006 0.0099 0 0.009 0.0098 0 0.0065 0.0013 0 0.0003 0.0005 0 0.0004
Punctate Nuclear 0.0037 0.0002 0.0025 0.0027 0.0008 0.004 0.0038 0.0003 0.0015 0.0058 0.0003 0.0007 0.0041 0.0002 0.001 0.0016 0.0005 0.0034
Vacuole 0.0025 0.0001 0.0004 0.0006 0.0005 0.0006 0.0063 0.0001 0.0007 0.0005 0.0001 0.0005 0.0007 0.0001 0.0001 0.0001 0.0001 0.0007
Vacuole Periphery 0.0009 0 0.0002 0.0002 0.0001 0.001 0.0017 0 0.0005 0.0002 0 0.0003 0.0002 0 0 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
308 973 1840 1537 1889 1838 2307 1203 2197 2811 4147 2740

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 838.63 985.39 1166.34 1250.27 910.78 1010.43 1227.32 1153.93 900.67 1001.76 1200.26 1207.97
Standard Deviation 117.81 147.26 141.27 170.12 138.37 143.34 156.15 168.48 137.97 145.20 152.77 176.02
Intensity Change Log 2 0.232660 0.475882 0.576133 0.149795 0.430337 0.341381 0.190113 0.452350 0.458688

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000186 0.000200 0.000100 0.003180 0.000099 0.000306 0.000122 0.004178 0.000111 0.000269 0.000112 0.003618
Bud Neck 0.003298 0.000873 0.002863 0.005763 0.001319 0.001427 0.003577 0.010031 0.001597 0.001235 0.003260 0.007637
Bud Site 0.008186 0.007036 0.004674 0.047915 0.003506 0.006461 0.006504 0.048314 0.004162 0.006660 0.005692 0.048090
Cell Periphery 0.000144 0.000055 0.000049 0.000120 0.000062 0.000060 0.000064 0.000106 0.000074 0.000058 0.000057 0.000114
Cytoplasm 0.670638* 0.803789* 0.644243* 0.585921* 0.743363* 0.783862* 0.653349* 0.480635* 0.733168* 0.790760* 0.649309* 0.539695*
Cytoplasmic Foci 0.155169 0.053906 0.004469 0.013808 0.081532 0.059629 0.007886 0.045635 0.091855 0.057648 0.006370 0.027782
Eisosomes 0.000061 0.000014 0.000009 0.000020 0.000024 0.000021 0.000016 0.000035 0.000029 0.000018 0.000013 0.000026
Endoplasmic Reticulum 0.001289 0.000621 0.001016 0.001047 0.000889 0.000506 0.001093 0.001418 0.000945 0.000546 0.001059 0.001210
Endosome 0.007795 0.003043 0.000748 0.008997 0.003484 0.004650 0.001389 0.027218 0.004088 0.004094 0.001105 0.016997
Golgi 0.000831 0.001057 0.000053 0.013904 0.000609 0.003039 0.000165 0.039326 0.000640 0.002353 0.000115 0.025066
Lipid Particles 0.002407 0.000472 0.000402 0.000925 0.001192 0.000760 0.000399 0.003144 0.001362 0.000660 0.000401 0.001900
Mitochondria 0.002193 0.001346 0.000401 0.001745 0.001512 0.001952 0.001614 0.024498 0.001607 0.001743 0.001076 0.011735
Mitotic Spindle 0.001453 0.002322 0.004642 0.073584 0.002907 0.004756 0.011918 0.098780 0.002703 0.003913 0.008689 0.084646
None 0.002270 0.003224 0.006765 0.007532 0.002232 0.003690 0.009324 0.003974 0.002237 0.003528 0.008189 0.005970
Nuclear Periphery 0.000190 0.000114 0.000818 0.000600 0.000198 0.000119 0.000571 0.000665 0.000197 0.000117 0.000681 0.000628
Nuclear Periphery Foci 0.002326 0.002732 0.001287 0.003875 0.001910 0.001427 0.000842 0.001777 0.001969 0.001879 0.001039 0.002954
Nucleolus 0.000318 0.000130 0.000129 0.000277 0.000353 0.000322 0.000226 0.000383 0.000348 0.000256 0.000183 0.000324
Nucleus 0.067684 0.068069 0.284268* 0.130078* 0.083243 0.063035 0.227851* 0.070134 0.081062 0.064777 0.252883* 0.103759
Peroxisomes 0.001350 0.000483 0.000086 0.003388 0.000378 0.001338 0.000483 0.005156 0.000514 0.001042 0.000307 0.004165
Vacuole 0.071591 0.049725 0.042884 0.096190 0.070813 0.062062 0.072335 0.127181 0.070922 0.057792 0.059268 0.109797
Vacuole Periphery 0.000621 0.000788 0.000094 0.001129 0.000376 0.000578 0.000272 0.007412 0.000410 0.000651 0.000193 0.003888

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.78 0.72 -2.28 -2.11 -2.39 -2.63 -6.13 -2.42 -1.72 -2.18 -3.14 -5.52 -3.52 -2.60 -3.23
Bud Neck 3.69 0.13 0.30 -7.44 0.22 -0.58 -5.50 -3.38 -3.14 -0.77 2.31 -4.97 -4.96 -6.37 -0.54
Bud Site 0.56 1.76 -1.17 -2.09 -4.23 -2.71 -0.58 -6.42 -4.35 -6.31 -2.41 -0.03 -6.89 -4.55 -7.37
Cell Periphery 7.39 7.33 3.74 -8.88 -9.49 0.65 0.92 -2.65 -3.26 -4.01 4.13 4.47 -3.63 -8.94 -10.16
Cytoplasm -8.16 7.83 13.61 36.74 10.42 -5.43 26.33 28.41 32.47 9.03 -8.65 29.18 38.01 49.50 13.98
Cytoplasmic Foci 10.63 16.84 16.52 17.17 -5.62 6.63 29.32 22.85 17.42 -9.66 10.87 33.23 30.10 25.94 -10.88
Eisosomes 9.43 9.57 8.03 -6.02 -7.43 1.82 6.89 -1.32 -2.78 -6.86 7.42 11.55 4.08 -4.44 -9.92
Endoplasmic Reticulum 2.24 -0.58 1.83 -1.26 6.49 3.79 -0.19 2.33 -1.81 1.76 4.15 -1.84 2.54 -2.64 4.62
Endosome 5.32 8.46 7.50 2.75 -1.17 -2.10 12.38 -1.08 0.15 -4.74 0.57 15.19 3.52 2.62 -4.73
Golgi -0.22 3.31 -0.65 -0.40 -1.96 -3.57 3.52 -3.20 -1.55 -3.56 -3.44 4.34 -3.24 -0.99 -4.05
Lipid Particles 4.11 3.89 2.01 -11.57 -14.50 3.08 4.54 -0.90 -3.17 -3.96 5.31 6.08 -0.55 -7.53 -9.40
Mitochondria 0.50 0.94 0.91 0.81 -0.70 -0.46 1.02 -1.67 -1.45 -2.49 -0.03 1.57 -0.31 -0.33 -2.40
Mitotic Spindle -0.77 -2.35 -7.82 -7.20 -6.95 -1.20 -4.28 -6.32 -5.79 -4.12 -1.08 -4.17 -9.60 -9.09 -7.39
None -1.67 -7.36 -8.06 -9.18 -1.71 -3.08 -13.82 -5.09 -1.03 9.70 -3.63 -16.34 -11.26 -6.70 5.85
Nuclear Periphery 2.29 -21.49 -22.67 -28.00 -1.21 5.15 -30.80 -13.93 -15.89 5.93 5.78 -37.39 -27.60 -31.30 2.36
Nuclear Periphery Foci -0.85 1.96 0.96 2.39 -1.44 2.83 5.19 1.57 -1.42 -3.99 0.48 4.52 0.84 0.40 -3.32
Nucleolus 3.88 3.00 1.89 -6.77 -3.80 0.24 1.10 1.16 1.27 0.32 0.81 1.41 1.20 0.57 -1.41
Nucleus -0.47 -37.00 -16.07 -21.89 25.77 6.58 -43.82 -4.02 -8.90 34.75 5.82 -60.25 -17.65 -23.65 39.52
Peroxisomes 2.32 3.53 2.40 0.35 -1.74 -3.01 1.67 -2.32 0.24 -2.79 -2.31 4.18 -1.15 0.99 -3.17
Vacuole 1.96 -0.96 -23.74 -31.36 -28.58 1.74 -10.85 -30.82 -31.72 -23.98 2.91 -8.49 -41.29 -44.38 -35.99
Vacuole Periphery -0.20 4.17 3.39 1.57 -0.88 -1.38 3.42 -2.31 -1.65 -3.19 -1.28 4.86 -1.01 0.25 -3.27