Standard name
Human Ortholog
Description Putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.07 0.12 0.11 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.95 0.96 0.94 0.87 0.88 0.77 0.78 0.83 0.68 0.68 0.99 0.99 0.99 0.91 0.88 0.9 0.97 0.92 0.87 0.9 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.23 0.11 0.27 0.36 0.41 0.47 0.41 0.56 0.54 0 0 0.05 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.08 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1
Bud 1 3 17 3 3 4 5 14 1 7 2 8 4 0 13 0 0 2 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 8 2 2 1 6 4 7 6 2 8 11 11 8 7 9 11 14 17 0 0 0 0 0 0
Cytoplasm 339 181 258 137 230 136 198 219 115 198 107 180 424 430 460 137 102 139 350 179 247 301 421 443
Endoplasmic Reticulum 8 0 0 0 1 1 1 0 2 0 1 2 0 1 0 5 3 7 2 0 3 15 20 19
Endosome 2 1 0 0 3 0 0 1 0 0 0 0 1 0 0 1 0 2 1 0 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 1 0 0 1 1 1
Mitochondria 1 2 20 33 28 43 81 117 70 98 88 143 3 1 22 3 3 2 0 3 18 5 4 8
Nucleus 0 3 0 0 1 0 0 0 0 1 0 1 0 0 0 2 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 7 1 0 0 5 1 1 13 0 4 0 3 1 1 2 13 9 7 0 7 6 5 5 7
Unique Cell Count 353 186 272 143 244 157 226 285 148 240 157 263 428 433 467 151 116 154 362 194 285 335 459 492
Labelled Cell Count 367 194 299 175 279 189 294 373 190 319 210 349 443 440 506 173 132 177 362 194 285 335 459 492


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 6.2 4.5 4.5 5.0 4.1 4.0 4.1 3.6 3.6 3.6 3.4 6.9 6.8 6.0 8.7 9.8 9.1 7.1 7.7 8.0
Std Deviation (1e-4) 1.0 1.0 0.9 1.2 1.3 0.8 1.1 1.2 1.2 1.1 1.3 1.2 0.9 0.8 0.8 1.3 2.2 2.3 1.0 1.2 1.3
Intensity Change (Log2) 0.0 0.14 -0.15 -0.16 -0.13 -0.33 -0.34 -0.34 -0.42 0.61 0.58 0.41 0.95 1.11 1.01 0.64 0.76 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -2.9 0 0 0 0 0 0 0 -4.0 -5.3 -2.3 -3.1 -2.8 -2.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.9
Cytoplasm 0.4 -0.3 -3.0 -2.9 -6.1 -5.3 -4.5 -7.5 -7.9 3.4 3.8 2.9 -1.6 -2.4 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.6 0 0 0 0 0 0 0 -4.8 -5.4 -1.5 -2.3 -1.8 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.6 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4739 4.0448 3.2976 3.1795 3.4346 3.7261 1.6616 2.8924 2.5682 2.4123 2.2177 2.2739 5.9303 6.649 6.3603 6.2752 6.003 6.0208
Actin 0.0477 0.0008 0.0088 0.0005 0.0008 0.0065 0.0134 0.0005 0.007 0.0062 0.0013 0.0007 0.0149 0.0017 0.0158 0.0007 0.0476 0.004
Bud 0.0009 0.0006 0.0006 0.0005 0.0003 0.0002 0.0014 0.0011 0.0011 0.0153 0.0003 0.0055 0.0022 0.0001 0.0054 0.0004 0.001 0.003
Bud Neck 0.0063 0.0003 0.0017 0.0005 0.0014 0.0012 0.0009 0.0003 0.0016 0.0015 0.0004 0.0058 0.0006 0.0002 0.0018 0.0004 0.0005 0.0009
Bud Periphery 0.0019 0.0002 0.0007 0.0003 0.0003 0.0002 0.0019 0.0005 0.0005 0.0097 0.0003 0.0032 0.0041 0 0.0032 0.0002 0.0012 0.001
Bud Site 0.0179 0.0033 0.0073 0.0008 0.0014 0.0001 0.0012 0.0069 0.0017 0.0027 0.0003 0.0007 0.0053 0.0007 0.0058 0.0005 0.0007 0.0008
Cell Periphery 0.003 0.0006 0.0005 0.0004 0.0002 0.0001 0.0003 0.0001 0.0002 0.0005 0.0001 0.0001 0.0008 0.0002 0.0007 0.0002 0.0001 0.0002
Cytoplasm 0.5632 0.9473 0.8853 0.932 0.8476 0.9296 0.7328 0.9245 0.8714 0.8199 0.8403 0.8992 0.8525 0.974 0.8705 0.9208 0.7547 0.9223
Cytoplasmic Foci 0.0215 0.0083 0.0169 0.0148 0.0356 0.0156 0.0303 0.0186 0.0218 0.0258 0.0223 0.0205 0.0171 0.0066 0.0121 0.0155 0.0305 0.0153
Eisosomes 0.0012 0 0.0002 0 0 0 0.0002 0 0.0001 0.0001 0 0 0.0004 0 0.001 0 0.0001 0
Endoplasmic Reticulum 0.0248 0.0046 0.0065 0.0044 0.0012 0.0108 0.012 0.0033 0.0065 0.0066 0.0097 0.0052 0.0107 0.0021 0.0039 0.0093 0.0091 0.0115
Endosome 0.0593 0.0094 0.0131 0.0136 0.044 0.0162 0.0814 0.024 0.0405 0.062 0.047 0.0371 0.0162 0.0043 0.009 0.0323 0.0521 0.0178
Golgi 0.0121 0.0011 0.0042 0.0009 0.0134 0.0021 0.0103 0.0015 0.0059 0.0068 0.0052 0.0021 0.0034 0.0006 0.0021 0.0018 0.0032 0.0028
Lipid Particles 0.0209 0.0002 0.0025 0.0004 0.0158 0.0002 0.0075 0.0004 0.0013 0.0024 0.006 0.0008 0.0016 0 0.0008 0.0003 0.0006 0.0002
Mitochondria 0.0176 0.0005 0.0058 0.0002 0.0143 0.0015 0.0332 0.0009 0.0154 0.0023 0.042 0.0014 0.0035 0.0001 0.0006 0.0003 0.0017 0.001
None 0.1161 0.017 0.0275 0.0209 0.0035 0.0107 0.0229 0.0094 0.0112 0.0075 0.0085 0.0092 0.052 0.0075 0.0626 0.0067 0.0881 0.0157
Nuclear Periphery 0.0297 0.0007 0.0043 0.0012 0.0003 0.0006 0.0234 0.0009 0.0014 0.0026 0.0021 0.0023 0.0044 0.0003 0.0006 0.0021 0.0013 0.0006
Nucleolus 0.0019 0.0001 0.0005 0.0001 0.0005 0 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003 0 0.0002 0 0.0001 0
Nucleus 0.0089 0.0012 0.0021 0.0012 0.0006 0.001 0.006 0.0013 0.0017 0.0023 0.0015 0.0011 0.0041 0.0005 0.0008 0.0011 0.001 0.0007
Peroxisomes 0.0141 0.0001 0.0027 0.0002 0.0106 0.0002 0.0023 0.0003 0.0009 0.0004 0.0006 0.0002 0.0013 0.0001 0.0006 0.0001 0.0015 0.0003
Punctate Nuclear 0.0179 0.0003 0.0036 0.0009 0.0016 0.0001 0.002 0.0011 0.0005 0.0008 0.0009 0.0005 0.0013 0.0001 0.0005 0.0003 0.0017 0.0002
Vacuole 0.0099 0.0033 0.0045 0.0057 0.0048 0.0024 0.0117 0.0035 0.0075 0.0202 0.0082 0.0033 0.0026 0.0007 0.0019 0.006 0.0023 0.0012
Vacuole Periphery 0.0031 0.0002 0.0008 0.0007 0.0016 0.0006 0.0044 0.0007 0.0016 0.004 0.0029 0.001 0.0006 0.0001 0.0003 0.001 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.3251 15.3704 14.8767 20.1402 13.6906 16.4373 19.764 22.0043 20.6809 21.3526
Translational Efficiency 0.4667 0.374 0.5299 0.3933 0.3914 0.49 0.4039 0.3023 0.3617 0.3532

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1156 1203 1389 1431 1360 1323 162 1080 2516 2526 1551 2511

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 606.08 889.11 1065.27 988.34 804.61 881.50 1053.37 940.88 713.39 885.12 1064.03 967.93
Standard Deviation 92.83 127.24 160.53 133.99 114.22 505.44 117.20 126.00 144.22 376.20 156.60 132.71
Intensity Change Log 2 0.552854 0.813639 0.705499 0.131671 0.388651 0.225721 0.327845 0.586737 0.451617

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000230 0.000307 0.000192 0.000572 0.000237 0.000320 0.000154 0.000761 0.000234 0.000314 0.000188 0.000653
Bud Neck 0.020214 0.005723 0.001369 0.004624 0.005655 0.006702 0.001404 0.002186 0.012345 0.006236 0.001373 0.003576
Bud Site 0.023198 0.036231 0.006728 0.029781 0.019733 0.025890 0.007932 0.020502 0.021325 0.030815 0.006854 0.025790
Cell Periphery 0.000542 0.000141 0.000100 0.000073 0.000145 0.000110 0.000111 0.000064 0.000327 0.000125 0.000101 0.000069
Cytoplasm 0.503737* 0.584451* 0.598456* 0.701902* 0.553659* 0.587342* 0.488700* 0.657360* 0.530722* 0.585965* 0.586992* 0.682744*
Cytoplasmic Foci 0.245670* 0.248926* 0.037476 0.058582 0.254049* 0.231324* 0.086657 0.069407 0.250199* 0.239707* 0.042613 0.063238
Eisosomes 0.000373 0.000038 0.000037 0.000017 0.000082 0.000040 0.000057 0.000015 0.000216 0.000039 0.000039 0.000016
Endoplasmic Reticulum 0.005442 0.001711 0.002471 0.000410 0.004651 0.001539 0.000847 0.000430 0.005015 0.001621 0.002301 0.000419
Endosome 0.026577 0.027464 0.007094 0.009020 0.026597 0.027650 0.012612 0.009603 0.026588 0.027561 0.007670 0.009271
Golgi 0.010280 0.013411 0.000187 0.007164 0.015540 0.015402 0.000186 0.004873 0.013123 0.014454 0.000187 0.006179
Lipid Particles 0.009711 0.001731 0.003231 0.000691 0.003913 0.002308 0.002234 0.000558 0.006577 0.002033 0.003127 0.000634
Mitochondria 0.015753 0.002586 0.000389 0.001647 0.007709 0.002448 0.000027 0.000404 0.011405 0.002514 0.000351 0.001112
Mitotic Spindle 0.000287 0.002651 0.001769 0.028613 0.001385 0.005905 0.000699 0.017577 0.000881 0.004355 0.001658 0.023866
None 0.005192 0.001138 0.002925 0.001546 0.000657 0.001822 0.000516 0.000751 0.002741 0.001496 0.002674 0.001204
Nuclear Periphery 0.000239 0.000053 0.001187 0.000269 0.000089 0.000311 0.000342 0.000162 0.000158 0.000188 0.001099 0.000223
Nuclear Periphery Foci 0.000338 0.000344 0.003338 0.001223 0.000315 0.000451 0.005321 0.000708 0.000326 0.000400 0.003545 0.001002
Nucleolus 0.000952 0.000248 0.000388 0.000568 0.000231 0.000421 0.000202 0.000173 0.000562 0.000338 0.000368 0.000398
Nucleus 0.036879 0.004955 0.108379 0.015640 0.010285 0.010419 0.023703 0.013622 0.022504 0.007817 0.099535 0.014772
Peroxisomes 0.003161 0.002255 0.000525 0.001271 0.001754 0.003602 0.000412 0.000899 0.002400 0.002960 0.000513 0.001111
Vacuole 0.080781 0.058042 0.223239* 0.132197 0.085298 0.069541 0.367626* 0.198260 0.083222 0.064064 0.238320* 0.160611
Vacuole Periphery 0.010445 0.007596 0.000519 0.004189 0.008016 0.006455 0.000257 0.001684 0.009132 0.006998 0.000491 0.003112

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.24 0.19 -0.91 -0.11 -0.94 -1.34 2.69 -0.81 -0.52 -1.09 -2.20 0.49 -1.10 -0.51 -1.21
Bud Neck 10.33 14.61 12.34 3.40 -2.46 -1.09 6.83 5.86 6.40 -1.98 7.35 15.62 12.85 6.47 -2.81
Bud Site -5.13 9.53 2.86 7.22 -5.08 -2.77 7.07 2.13 4.66 -3.68 -5.64 10.78 3.33 8.29 -6.07
Cell Periphery 6.98 7.96 8.74 6.39 6.83 2.65 4.16 6.54 3.96 3.25 7.33 8.69 10.28 7.43 6.30
Cytoplasm -7.65 -6.95 -12.71 -4.65 -5.04 -3.31 4.51 -6.52 -3.37 -7.33 -7.53 -3.71 -13.30 -5.75 -6.86
Cytoplasmic Foci -0.40 38.95 35.32 32.65 -5.48 3.05 20.77 33.36 29.09 1.79 1.97 52.57 48.75 43.96 -6.45
Eisosomes 8.45 8.56 9.04 6.95 6.56 7.05 4.28 12.46 9.37 8.66 9.45 9.60 10.80 11.37 8.48
Endoplasmic Reticulum 4.31 4.13 6.61 3.85 11.47 3.84 5.12 5.76 3.81 3.90 5.73 5.37 8.71 5.46 11.44
Endosome -0.40 13.49 13.93 14.92 2.29 -0.48 9.46 13.57 12.77 2.25 -0.62 19.15 19.67 19.58 2.00
Golgi -3.63 16.68 6.93 9.76 -2.82 0.14 20.37 10.04 8.90 -2.47 -1.69 26.05 12.35 13.00 -3.73
Lipid Particles 11.11 9.17 12.82 5.49 6.14 3.32 5.14 13.92 5.15 3.19 10.93 8.86 16.82 6.84 6.79
Mitochondria 8.00 9.77 9.49 3.51 -1.27 3.34 4.98 4.83 6.63 -2.77 7.78 9.96 9.79 6.33 -1.04
Mitotic Spindle -2.24 -3.28 -6.98 -5.48 -5.86 -2.66 0.87 -4.05 -2.32 -4.31 -3.41 -1.95 -7.79 -5.42 -6.83
None 3.68 2.17 3.73 -0.34 1.57 -1.99 1.18 -0.34 1.87 -1.10 1.96 0.58 3.45 1.23 1.86
Nuclear Periphery 5.00 -8.74 -2.80 -9.61 7.53 -0.96 -7.50 -3.49 0.43 4.58 -0.25 -10.14 -4.86 -0.93 8.50
Nuclear Periphery Foci 0.05 -13.66 -6.80 -9.28 10.67 -0.27 -8.33 -2.26 -1.37 7.90 -0.22 -15.13 -6.19 -5.15 13.05
Nucleolus 3.09 2.77 2.46 -0.55 0.12 -1.55 1.89 1.64 2.44 -0.75 1.78 2.42 2.49 0.97 0.77
Nucleus 11.99 -15.06 3.96 -21.36 21.04 -0.16 -3.65 -6.68 -4.69 1.63 9.81 -20.57 0.06 -15.26 21.74
Peroxisomes 2.31 7.39 6.51 6.22 -1.89 -3.04 10.86 5.09 4.57 -2.77 -1.59 9.32 8.25 6.28 -2.24
Vacuole 5.27 -20.81 -21.19 -25.79 1.16 3.81 -14.48 -20.41 -22.68 7.18 6.43 -24.75 -29.46 -34.11 2.78
Vacuole Periphery 2.68 10.94 8.34 7.88 -2.31 1.92 12.49 9.47 8.16 -1.93 3.25 16.14 12.14 10.94 -2.75