Standard name | |
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Human Ortholog | |
Description | Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 7 | 8 |
Bud | 3 | 1 | – | 0 | 2 | 3 | 3 | 2 | 1 | 1 | 4 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 5 | 7 |
Bud Neck | 0 | 0 | – | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 1 | 0 | 0 | 3 | 6 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 4 |
Cytoplasm | 251 | 98 | – | 45 | 204 | 104 | 172 | 169 | 131 | 167 | 152 | 143 | 302 | 373 | 384 | 231 | 240 | 280 | 147 | 63 | 13 | 79 | 214 | 211 |
Endoplasmic Reticulum | 2 | 2 | – | 0 | 2 | 4 | 0 | 0 | 2 | 0 | 1 | 1 | 3 | 2 | 1 | 19 | 16 | 18 | 2 | 0 | 0 | 0 | 3 | 3 |
Endosome | 1 | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 4 | 18 | 2 | 0 | 1 | 3 | 5 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 5 | 1 |
Mitochondria | 35 | 6 | – | 0 | 1 | 5 | 14 | 6 | 9 | 8 | 11 | 6 | 0 | 0 | 2 | 4 | 4 | 6 | 36 | 6 | 6 | 11 | 30 | 25 |
Nucleus | 0 | 2 | – | 0 | 2 | 11 | 8 | 7 | 7 | 7 | 1 | 4 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 2 | 2 | – | 0 | 0 | 1 | 4 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 7 | 4 |
Vac/Vac Membrane | 3 | 2 | – | 1 | 35 | 24 | 27 | 36 | 27 | 29 | 5 | 18 | 0 | 0 | 0 | 21 | 19 | 22 | 71 | 37 | 4 | 27 | 24 | 20 |
Unique Cell Count | 280 | 108 | 45 | 228 | 130 | 205 | 196 | 157 | 192 | 163 | 163 | 305 | 375 | 391 | 252 | 258 | 307 | 301 | 117 | 27 | 129 | 323 | 314 | |
Labelled Cell Count | 301 | 114 | 48 | 251 | 154 | 230 | 224 | 178 | 215 | 177 | 179 | 306 | 376 | 392 | 281 | 288 | 339 | 301 | 117 | 27 | 129 | 323 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 4.1 | – | 4.6 | 5.6 | 4.6 | 4.5 | 4.9 | 4.4 | 4.5 | 4.4 | 4.5 | 5.3 | 5.3 | 5.4 | 5.6 | 6.3 | 5.8 | 5.0 | 5.3 | 5.8 |
Std Deviation (1e-4) | 0.6 | 0.8 | – | 2.1 | 1.4 | 1.2 | 1.2 | 1.2 | 1.1 | 0.9 | 1.5 | 1.0 | 1.3 | 1.4 | 1.9 | 1.2 | 3.0 | 1.6 | 1.7 | 1.8 | 2.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7853 | -0.6661 | -0.8682 | -0.4337 | -0.5218 | -0.9234 | 0.2096 | 0.0722 | -0.1155 | 0.3022 | 0.1794 | -0.1278 | -0.8987 | -0.8787 | -1.0545 | -0.8127 | -1.3151 | -0.9764 |
Actin | 0.0294 | 0.001 | 0.0158 | 0.011 | 0.0448 | 0.0005 | 0.0294 | 0.0006 | 0.0072 | 0.0263 | 0.0333 | 0.0078 | 0.0197 | 0.0002 | 0.0071 | 0.0244 | 0.0001 | 0.0005 |
Bud | 0.001 | 0.0074 | 0.0022 | 0.0461 | 0.0002 | 0.0001 | 0.0004 | 0.001 | 0.0001 | 0.0007 | 0.0011 | 0.0019 | 0.0008 | 0.0001 | 0.0018 | 0.018 | 0.0001 | 0.0001 |
Bud Neck | 0.007 | 0.0002 | 0.0005 | 0.0032 | 0.0006 | 0.0157 | 0.0123 | 0.0003 | 0.0001 | 0.0007 | 0.0086 | 0.001 | 0.0032 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.0022 |
Bud Periphery | 0.0012 | 0.0018 | 0.0011 | 0.0093 | 0.0003 | 0.0004 | 0.0007 | 0.0003 | 0.0002 | 0.0011 | 0.0012 | 0.0009 | 0.0014 | 0.0001 | 0.0024 | 0.0031 | 0.0001 | 0.0003 |
Bud Site | 0.0016 | 0.017 | 0.0054 | 0.0049 | 0.0006 | 0 | 0.0161 | 0.0086 | 0.0004 | 0.0042 | 0.011 | 0.0004 | 0.0066 | 0.0004 | 0.008 | 0.0012 | 0 | 0.0002 |
Cell Periphery | 0.0002 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0204 | 0.0225 | 0.0291 | 0.0214 | 0.0158 | 0.062 | 0.0279 | 0.0577 | 0.0468 | 0.0297 | 0.0545 | 0.0986 | 0.1073 | 0.1006 | 0.138 | 0.1763 | 0.1245 | 0.2003 |
Cytoplasmic Foci | 0.0291 | 0.0084 | 0.0081 | 0.0138 | 0.0219 | 0.013 | 0.02 | 0.0129 | 0.0151 | 0.0316 | 0.021 | 0.0136 | 0.0416 | 0.0099 | 0.0334 | 0.0149 | 0.0099 | 0.0332 |
Eisosomes | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.003 | 0.0005 | 0.0012 | 0.0021 | 0.003 | 0.0004 | 0.0022 | 0.0014 | 0.0017 | 0.0023 | 0.0011 | 0.001 | 0.0046 | 0.0011 | 0.0021 | 0.001 | 0.0008 | 0.0022 |
Endosome | 0.0129 | 0.0016 | 0.0013 | 0.0148 | 0.0188 | 0.0082 | 0.0158 | 0.0059 | 0.0106 | 0.065 | 0.0219 | 0.0059 | 0.0541 | 0.0081 | 0.0354 | 0.0156 | 0.0043 | 0.023 |
Golgi | 0.0031 | 0.0003 | 0.0009 | 0.0056 | 0.0064 | 0.0001 | 0.0051 | 0.0004 | 0.001 | 0.0144 | 0.0149 | 0.0005 | 0.0055 | 0.0002 | 0.0036 | 0.0019 | 0.0001 | 0.0005 |
Lipid Particles | 0.0182 | 0.0008 | 0.0006 | 0.0518 | 0.0118 | 0.0006 | 0.0166 | 0.0007 | 0.0045 | 0.0185 | 0.0275 | 0.0007 | 0.0147 | 0.0007 | 0.0059 | 0.0014 | 0.0005 | 0.0022 |
Mitochondria | 0.0024 | 0.0013 | 0.0015 | 0.0141 | 0.0016 | 0.0005 | 0.0015 | 0.0005 | 0.0012 | 0.0226 | 0.035 | 0.001 | 0.0042 | 0.0004 | 0.0051 | 0.0015 | 0.0007 | 0.0013 |
None | 0.8149 | 0.9217 | 0.9152 | 0.7328 | 0.845 | 0.8906 | 0.8154 | 0.8944 | 0.8873 | 0.7471 | 0.7174 | 0.8492 | 0.6754 | 0.8574 | 0.7099 | 0.7099 | 0.8529 | 0.6961 |
Nuclear Periphery | 0.0163 | 0.0008 | 0.0012 | 0.0134 | 0.0082 | 0.0007 | 0.0069 | 0.0011 | 0.0006 | 0.0036 | 0.0009 | 0.0011 | 0.0095 | 0.0007 | 0.0023 | 0.0014 | 0.0007 | 0.007 |
Nucleolus | 0.0026 | 0.0014 | 0.0014 | 0.0085 | 0.0007 | 0.0003 | 0.0009 | 0.0006 | 0.0004 | 0.0008 | 0.0054 | 0.0008 | 0.0017 | 0.0005 | 0.0013 | 0.0007 | 0.0002 | 0.0006 |
Nucleus | 0.0068 | 0.0024 | 0.0029 | 0.0253 | 0.0014 | 0.0009 | 0.0031 | 0.0029 | 0.0008 | 0.0017 | 0.0135 | 0.0033 | 0.0058 | 0.002 | 0.0027 | 0.0056 | 0.0009 | 0.0074 |
Peroxisomes | 0.0084 | 0.0057 | 0.0009 | 0.0125 | 0.0035 | 0.0004 | 0.0087 | 0.0004 | 0.0036 | 0.008 | 0.0241 | 0.0005 | 0.0154 | 0.0002 | 0.0137 | 0.0044 | 0.0001 | 0.0013 |
Punctate Nuclear | 0.018 | 0.0031 | 0.0094 | 0.0032 | 0.0135 | 0.0008 | 0.013 | 0.0035 | 0.0008 | 0.0045 | 0.0031 | 0.0059 | 0.0076 | 0.0086 | 0.0034 | 0.0011 | 0.0006 | 0.0026 |
Vacuole | 0.0024 | 0.001 | 0.0006 | 0.0032 | 0.0008 | 0.004 | 0.0017 | 0.006 | 0.0161 | 0.0135 | 0.0029 | 0.0053 | 0.0176 | 0.0077 | 0.0207 | 0.0158 | 0.0027 | 0.0175 |
Vacuole Periphery | 0.0008 | 0.0002 | 0.0002 | 0.0019 | 0.0006 | 0.0005 | 0.0007 | 0.0004 | 0.0013 | 0.0029 | 0.0007 | 0.0004 | 0.0028 | 0.0006 | 0.0022 | 0.0013 | 0.0004 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1006.183 | 1073.2862 | 1039.6255 | 857.0846 | 695.563 | 1017.3329 | 1091.8156 | 988.9671 | 1013.4425 | 796.1159 |
Translational Efficiency | 1.2693 | 1.2395 | 1.2842 | 1.4313 | 1.3456 | 1.285 | 1.4733 | 1.4535 | 1.3793 | 1.3617 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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