Standard name
Description Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.91 1.0 0.89 0.8 0.84 0.86 0.83 0.87 0.93 0.88 0.99 0.99 0.98 0.92 0.93 0.91 0.49 0.54 0.52 0.62 0.67 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.06 0 0 0 0.07 0 0.06 0 0.07 0 0 0 0 0 0 0 0.12 0.06 0.26 0.09 0.09 0.08
Nucleus 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.15 0.18 0.13 0.18 0.17 0.15 0 0.11 0 0 0 0.08 0.07 0.07 0.24 0.32 0.18 0.22 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 0 0 7 8
Bud 3 1 0 2 3 3 2 1 1 4 2 0 0 0 1 0 1 2 0 0 1 5 7
Bud Neck 0 0 2 0 1 1 0 1 0 0 1 0 0 0 0 1 2 6 1 0 0 3 6
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 3 0 1 1 0 0 0 0 1 2 2 0 0 0 0 2 4
Cytoplasm 251 98 45 204 104 172 169 131 167 152 143 302 373 384 231 240 280 147 63 13 79 214 211
Endoplasmic Reticulum 2 2 0 2 4 0 0 2 0 1 1 3 2 1 19 16 18 2 0 0 0 3 3
Endosome 1 0 0 3 1 0 0 0 0 0 0 0 0 2 2 2 4 18 2 0 1 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 3 0 0 0 0 5 1
Mitochondria 35 6 0 1 5 14 6 9 8 11 6 0 0 2 4 4 6 36 6 6 11 30 25
Nucleus 0 2 0 2 11 8 7 7 7 1 4 0 0 1 2 2 1 0 1 0 0 1 1
Nuclear Periphery 2 2 0 0 1 4 1 0 2 2 2 1 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 3 0 0 0 7 4
Vac/Vac Membrane 3 2 1 35 24 27 36 27 29 5 18 0 0 0 21 19 22 71 37 4 27 24 20
Unique Cell Count 280 108 45 228 130 205 196 157 192 163 163 305 375 391 252 258 307 301 117 27 129 323 314
Labelled Cell Count 301 114 48 251 154 230 224 178 215 177 179 306 376 392 281 288 339 301 117 27 129 323 314


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.1 4.6 5.6 4.6 4.5 4.9 4.4 4.5 4.4 4.5 5.3 5.3 5.4 5.6 6.3 5.8 5.0 5.3 5.8
Std Deviation (1e-4) 0.6 0.8 2.1 1.4 1.2 1.2 1.2 1.1 0.9 1.5 1.0 1.3 1.4 1.9 1.2 3.0 1.6 1.7 1.8 2.2
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bgl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available