Standard name
Description Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partially complement yeast zuo1 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.08 0.09 0.11 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.97 0.9 0.86 0.7 0.59 0.66 0.63 0.63 0.57 0.99 0.98 0.97 0.97 0.98 0.98 0.94 0.95 0.93 0.94 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0.08 0.09 0.23 0.28 0.21 0.22 0.22 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.07 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 14 26 29 36 48 55 61 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 1 3 2 3 7 3 7 0 0 0 3 1 0 1 0 5 2 6 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 11 9 8 17 32 62 59 77 57 78 4 3 6 4 2 1 0 0 4 0 0 0
Cytoplasm 177 251 614 479 446 518 481 450 433 442 403 403 322 389 421 341 457 390 168 247 620 357 414 396
Endoplasmic Reticulum 0 3 14 7 39 55 156 215 138 154 144 175 1 8 6 13 14 17 3 4 19 1 6 8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Golgi 0 0 0 2 7 9 7 56 21 19 31 38 0 1 3 0 1 3 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 10 31 16 25 43 39 34 0 0 0 0 4 1 0 0 2 0 0 1
Nucleus 0 0 0 0 0 0 1 1 1 0 1 1 0 0 0 0 0 0 0 0 0 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 1 2 3 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 1 1 2 6 3 1 1 7 2 1 1 5 5 8 1 1 2 8 8 9
Unique Cell Count 177 254 630 493 495 604 688 762 656 698 641 709 325 396 432 350 467 397 180 261 668 381 445 435
Labelled Cell Count 177 257 639 499 502 626 741 840 719 791 735 805 329 402 437 366 484 420 180 261 668 381 445 435


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 88.7 104.0 78.0 68.2 58.1 53.7 48.3 48.6 46.1 44.2 43.8 42.0 87.2 86.7 85.1 113.4 106.1 114.9 88.8 96.7 103.1
Std Deviation (1e-4) 18.9 24.9 18.4 15.4 12.1 10.4 10.6 11.5 11.2 10.0 10.4 10.0 25.2 22.4 22.8 43.6 38.6 43.4 26.5 30.6 34.0
Intensity Change (Log2) – – – -0.19 -0.43 -0.54 -0.69 -0.68 -0.76 -0.82 -0.83 -0.89 0.16 0.15 0.13 0.54 0.44 0.56 0.19 0.31 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 3.8 4.9 4.9 6.0 6.7 7.5 7.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -0.2 1.3 3.0 5.3 5.7 6.8 5.7 6.8 -0.6 -1.3 -0.5 -0.7 -2.0 0
Cytoplasm -0.3 -5.3 -7.5 -13.3 -16.8 -14.5 -15.4 -15.4 -17.4 1.7 0.8 0 0 0.4 0.8
Endoplasmic Reticulum -1.0 4.4 5.3 11.1 13.0 10.4 10.9 10.9 11.8 -2.3 -0.2 -1.0 1.4 0.8 1.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 6.9 4.5 4.2 5.6 5.9 0 0 0 0 0 0
Mitochondria 0 0 0 5.4 3.7 4.9 6.3 6.3 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zuo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available