Standard name
Human Ortholog
Description Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partially complement yeast zuo1 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.08 0.09 0.11 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.97 0.9 0.86 0.7 0.59 0.66 0.63 0.63 0.57 0.99 0.98 0.97 0.97 0.98 0.98 0.94 0.95 0.93 0.94 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0.08 0.09 0.23 0.28 0.21 0.22 0.22 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.07 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 14 26 29 36 48 55 61 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 1 3 2 3 7 3 7 0 0 0 3 1 0 1 0 5 2 6 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 11 9 8 17 32 62 59 77 57 78 4 3 6 4 2 1 0 0 4 0 0 0
Cytoplasm 177 251 614 479 446 518 481 450 433 442 403 403 322 389 421 341 457 390 168 247 620 357 414 396
Endoplasmic Reticulum 0 3 14 7 39 55 156 215 138 154 144 175 1 8 6 13 14 17 3 4 19 1 6 8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Golgi 0 0 0 2 7 9 7 56 21 19 31 38 0 1 3 0 1 3 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 10 31 16 25 43 39 34 0 0 0 0 4 1 0 0 2 0 0 1
Nucleus 0 0 0 0 0 0 1 1 1 0 1 1 0 0 0 0 0 0 0 0 0 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 1 2 3 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 1 1 2 6 3 1 1 7 2 1 1 5 5 8 1 1 2 8 8 9
Unique Cell Count 177 254 630 493 495 604 688 762 656 698 641 709 325 396 432 350 467 397 180 261 668 381 445 435
Labelled Cell Count 177 257 639 499 502 626 741 840 719 791 735 805 329 402 437 366 484 420 180 261 668 381 445 435


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 88.7 104.0 78.0 68.2 58.1 53.7 48.3 48.6 46.1 44.2 43.8 42.0 87.2 86.7 85.1 113.4 106.1 114.9 88.8 96.7 103.1
Std Deviation (1e-4) 18.9 24.9 18.4 15.4 12.1 10.4 10.6 11.5 11.2 10.0 10.4 10.0 25.2 22.4 22.8 43.6 38.6 43.4 26.5 30.6 34.0
Intensity Change (Log2) – – – -0.19 -0.43 -0.54 -0.69 -0.68 -0.76 -0.82 -0.83 -0.89 0.16 0.15 0.13 0.54 0.44 0.56 0.19 0.31 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 3.8 4.9 4.9 6.0 6.7 7.5 7.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -0.2 1.3 3.0 5.3 5.7 6.8 5.7 6.8 -0.6 -1.3 -0.5 -0.7 -2.0 0
Cytoplasm -0.3 -5.3 -7.5 -13.3 -16.8 -14.5 -15.4 -15.4 -17.4 1.7 0.8 0 0 0.4 0.8
Endoplasmic Reticulum -1.0 4.4 5.3 11.1 13.0 10.4 10.9 10.9 11.8 -2.3 -0.2 -1.0 1.4 0.8 1.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 6.9 4.5 4.2 5.6 5.9 0 0 0 0 0 0
Mitochondria 0 0 0 5.4 3.7 4.9 6.3 6.3 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 83.5658 113.0872 96.0038 95.5762 88.3054 99.7935 79.7312 119.6097 111.0206 96.5534 97.6014 91.61 93.7335 115.9042 107.2458 104.5823 101.2402 108.3078
Actin 0.0054 0.0001 0.0003 0.0005 0.001 0.0013 0.0075 0.0001 0.0005 0.0001 0.0003 0.0036 0.0066 0.0003 0.0054 0.0011 0.0001 0.0011
Bud 0.0015 0.0005 0.0014 0.0016 0.0008 0.002 0.0024 0.0003 0.0007 0.0014 0.0014 0.0025 0.0019 0.0003 0.0013 0.0009 0.0007 0.001
Bud Neck 0.0007 0.0007 0.0008 0.0006 0.0011 0.0022 0.0009 0.0003 0.0005 0.0005 0.0007 0.0012 0.0006 0.0006 0.0009 0.0008 0.0005 0.0013
Bud Periphery 0.0017 0.0003 0.0012 0.0025 0.0004 0.0018 0.0038 0.0001 0.0003 0.0011 0.0029 0.002 0.0025 0.0003 0.0016 0.0003 0.0006 0.0008
Bud Site 0.0008 0.0003 0.0005 0.0004 0.0004 0.0003 0.0022 0.0002 0.0003 0.0002 0.0003 0.001 0.0016 0.0002 0.0009 0.0002 0.0001 0.0002
Cell Periphery 0.0255 0.0045 0.0114 0.0155 0.0061 0.0079 0.0239 0.0096 0.0078 0.0181 0.0059 0.0146 0.0143 0.0109 0.0083 0.0085 0.0009 0.0022
Cytoplasm 0.6636 0.9255 0.8059 0.7288 0.7726 0.7898 0.6448 0.9389 0.8974 0.6342 0.7549 0.7151 0.7289 0.9202 0.8614 0.699 0.8024 0.7427
Cytoplasmic Foci 0.0055 0.0009 0.0087 0.0013 0.0067 0.0092 0.008 0.0008 0.0052 0.0059 0.0347 0.03 0.0043 0.0013 0.0056 0.0055 0.0056 0.0108
Eisosomes 0.0001 0 0 0.0001 0.0001 0 0.0002 0 0.0002 0 0.0009 0.0005 0.001 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0387 0.0124 0.0087 0.0031 0.0252 0.0431 0.0381 0.0049 0.0093 0.0034 0.0032 0.0175 0.0615 0.0158 0.0166 0.0077 0.0062 0.0771
Endosome 0.0114 0.0032 0.0069 0.008 0.0256 0.0244 0.0199 0.0013 0.0035 0.0319 0.0158 0.0334 0.012 0.0031 0.0087 0.014 0.054 0.033
Golgi 0.0015 0.0004 0.0004 0.0007 0.003 0.0029 0.0055 0.0001 0.0006 0.0048 0.0016 0.0079 0.0065 0.001 0.0021 0.0083 0.0019 0.0104
Lipid Particles 0.0022 0.0005 0.0014 0.0006 0.0042 0.0009 0.0106 0.0014 0.0039 0.0024 0.0201 0.0106 0.0027 0.0008 0.0023 0.0014 0.0001 0.002
Mitochondria 0.0074 0.0006 0.0017 0.0089 0.0241 0.0133 0.013 0.0002 0.0036 0.0032 0.0043 0.0145 0.0192 0.0015 0.0052 0.0009 0.001 0.0116
None 0.0024 0.0001 0.0017 0.0004 0.0027 0.0034 0.0049 0.0007 0.0018 0.0042 0.0195 0.0086 0.0046 0.0002 0.0007 0.0005 0.0002 0.0033
Nuclear Periphery 0.0225 0.002 0.0054 0.0052 0.017 0.0077 0.0196 0.0022 0.0037 0.0061 0.003 0.009 0.0117 0.0032 0.0033 0.0057 0.0044 0.0071
Nucleolus 0.004 0.0001 0.0015 0.0006 0.0025 0.0015 0.0074 0.0002 0.0036 0.0016 0.0022 0.0104 0.0095 0.0001 0.0005 0.0019 0 0.0374
Nucleus 0.0099 0.0013 0.0047 0.0042 0.0113 0.0038 0.0105 0.0015 0.0024 0.0067 0.0041 0.0084 0.0047 0.0013 0.0017 0.0045 0.0021 0.0119
Peroxisomes 0.0007 0 0.0002 0 0.0013 0.0003 0.0018 0 0.0003 0.0002 0.0022 0.0013 0.0008 0 0.0012 0.0001 0 0.0002
Punctate Nuclear 0.0005 0 0.0012 0.0001 0.0024 0.0004 0.0019 0 0.001 0.0001 0.0025 0.008 0.0002 0 0.0037 0.0001 0 0.0006
Vacuole 0.1568 0.0416 0.1118 0.1611 0.0705 0.0551 0.1421 0.0354 0.0473 0.2238 0.0919 0.0773 0.0842 0.0356 0.0556 0.2062 0.0865 0.0241
Vacuole Periphery 0.037 0.005 0.024 0.056 0.021 0.0286 0.031 0.002 0.0063 0.05 0.0277 0.0226 0.0208 0.0033 0.0131 0.0326 0.0328 0.0212

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 547.0411 706.0696 717.3175 540.9957 634.5457 637.7908 560.133 494.2464 578.5943 437.1301
Translational Efficiency 2.2173 2.0879 1.921 2.9479 2.2163 1.8102 1.8728 2.4696 1.8555 2.2954

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partially complement yeast zuo1 null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Zuo1

Zuo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zuo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available