Standard name
Human Ortholog
Description Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.09 0.08 0.16 0.1 0.11 0.11 0.09 0.11 0.1 0.13 0.17 0.21 0.17 0.35 0 0.05 0.06 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.2 0.36 0.08 0.4 0.38 0.45 0.63 0.68 0.69 0.69 0 0 0 0 0 0 0 0 0 0
Nucleus 0.69 0.56 0.59 0.56 0.39 0.46 0.56 0.58 0.44 0.44 0.42 0.36 0.42 0.49 0.61 0.25 0.77 0.76 0.62 0.76 0.71 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.46 0.23 0.23 0.45 0.28 0.2 0.16 0.17 0.12 0.13 0.17 0.48 0.32 0.21 0.45 0.11 0.09 0.19 0.11 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0
Bud 0 1 3 0 2 1 4 7 3 2 9 16 0 0 0 0 4 5 7 11 14 9
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1
Bud Site 0 0 0 0 0 1 3 17 6 3 9 12 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12 15 24 15 44 30 38 56 25 37 31 59 40 63 56 7 11 10 20 5 8 7
Endoplasmic Reticulum 0 0 1 1 2 0 0 0 0 0 0 0 0 2 2 0 0 0 3 0 1 2
Endosome 5 1 10 1 6 1 5 4 0 1 0 0 1 1 2 0 5 5 2 1 6 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 4
Mitochondria 3 4 54 64 22 117 132 234 179 235 218 313 2 0 9 0 1 2 7 0 3 2
Nucleus 248 107 162 100 104 135 196 300 124 152 133 165 97 145 196 5 323 171 214 177 229 215
Nuclear Periphery 1 0 0 0 1 0 2 1 5 3 1 4 1 1 0 0 5 0 3 0 1 2
Nucleolus 1 0 0 1 1 0 0 5 0 1 0 1 1 1 2 0 12 3 5 4 7 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 7 4 5 2 4 4
Vac/Vac Membrane 139 88 63 41 122 82 69 82 47 41 41 77 110 95 66 9 44 21 66 26 39 55
Unique Cell Count 362 192 274 177 270 291 352 519 282 344 314 453 230 294 320 20 420 226 343 233 324 324
Labelled Cell Count 409 217 318 223 304 367 449 708 389 475 446 648 252 308 333 21 420 226 343 233 324 324


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.6 5.1 4.9 5.9 5.0 4.8 4.7 4.0 3.7 3.6 3.5 7.8 8.0 6.7 14.3 7.6 8.1 8.9
Std Deviation (1e-4) 1.2 1.3 0.9 1.0 1.3 0.8 1.1 1.3 1.1 1.0 1.0 1.1 1.6 1.7 1.5 3.8 1.4 1.7 1.7
Intensity Change (Log2) -0.06 0.2 -0.04 -0.11 -0.14 -0.37 -0.46 -0.52 -0.54 0.61 0.64 0.37 1.47 0.56 0.66 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 2.7 0.6 0.8 0.9 0 0.8 0.5 1.8 2.9 4.2 3.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 -1.0 -2.8 0 0 0 0 0 0 -2.5 -2.9 -2.6 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.9 -3.9 5.3 0 0 0 0 0 0 -6.7 -8.0 -6.7 0
Nucleus -0.6 -4.8 -3.0 -0.9 -0.4 -3.6 -3.7 -4.1 -6.0 -3.8 -2.3 0.5 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.5 1.4 -1.0 -2.5 -1.9 -3.7 -3.1 -2.0 5.8 2.5 -0.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5385 5.6897 5.1172 4.2798 3.8645 5.4419 5.6034 7.301 6.2964 6.6106 5.4918 6.6648 7.2085 7.9574 6.7637 6.8374 6.8298 6.8561
Actin 0.0286 0.0005 0.0106 0.003 0.0016 0.0002 0.0489 0.0096 0.0019 0.0048 0.0004 0.0002 0.0152 0.0001 0.0104 0.0031 0.0149 0.0015
Bud 0.0006 0.0002 0.0001 0.0002 0.0004 0.0001 0.0008 0.0004 0 0.0003 0 0.0001 0.0009 0.0001 0.0003 0.0003 0.0002 0.0002
Bud Neck 0.0013 0.0005 0.0003 0.0015 0.0008 0.0041 0.0036 0.0012 0.0002 0.0007 0.0002 0.0021 0.0023 0.0094 0.0006 0.0017 0.001 0.0016
Bud Periphery 0.0011 0.0001 0.0001 0.0002 0.0014 0.0006 0.0016 0.0003 0 0.001 0 0.0002 0.001 0.0001 0.0002 0.0006 0.0011 0.0003
Bud Site 0.0026 0.0009 0.0003 0.0006 0.001 0.0001 0.0027 0.0078 0.0002 0.0007 0.0001 0.0001 0.0129 0.0058 0.0022 0.0005 0.0006 0.0002
Cell Periphery 0.0003 0 0 0.0001 0.0005 0.0002 0.0008 0.0001 0 0.0003 0 0.0001 0.0003 0 0 0.0001 0.0003 0
Cytoplasm 0.0337 0.0148 0.0033 0.027 0.0027 0.0063 0.0141 0.0067 0.0063 0.0131 0.0007 0.0095 0.0278 0.0015 0.0078 0.0054 0.0105 0.0028
Cytoplasmic Foci 0.0132 0.0031 0.001 0.0079 0.0005 0.0002 0.0072 0.0039 0.0055 0.0006 0.022 0.0002 0.0122 0 0.0079 0.0223 0.0087 0.0016
Eisosomes 0.0002 0 0 0.0001 0.0001 0 0.0003 0 0 0.0001 0 0 0.0005 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0104 0.001 0.0018 0.0017 0.0069 0.0008 0.0056 0.0002 0.0004 0.0028 0.0001 0.001 0.0056 0.0017 0.0008 0.0008 0.0018 0.0011
Endosome 0.0225 0.004 0.0022 0.0166 0.0151 0.0036 0.025 0.0012 0.0037 0.0123 0.0225 0.0007 0.0295 0.0001 0.0121 0.0337 0.0057 0.0083
Golgi 0.0083 0.0031 0.0017 0.0222 0.0018 0.0001 0.0079 0.0035 0.0012 0.0015 0.022 0 0.0069 0 0.0038 0.0218 0.002 0.0015
Lipid Particles 0.011 0.0006 0.0002 0.0287 0.0026 0.0003 0.0158 0.0005 0.0012 0.0013 0.0072 0 0.0139 0 0.0015 0.0275 0.0023 0.0008
Mitochondria 0.0068 0.0013 0.0026 0.0107 0.0316 0.0172 0.0147 0.0006 0.0005 0.0169 0.0042 0.0003 0.0087 0.0001 0.0011 0.015 0.043 0.0067
None 0.0081 0.0006 0.0003 0.0004 0.0006 0.0003 0.0017 0.0001 0.0012 0.0013 0.0001 0.0004 0.013 0 0.0086 0.0003 0.0043 0.0006
Nuclear Periphery 0.0245 0.0181 0.027 0.0484 0.0723 0.0166 0.0312 0.0117 0.0068 0.0188 0.0017 0.0077 0.0225 0.0115 0.0132 0.0169 0.0122 0.0102
Nucleolus 0.0023 0.0032 0.0024 0.0023 0.0047 0.0057 0.0034 0.0018 0.0017 0.0034 0.0024 0.0045 0.0031 0.0024 0.0024 0.0012 0.0057 0.0047
Nucleus 0.7778 0.9423 0.9366 0.8118 0.8337 0.9359 0.7718 0.9376 0.9647 0.909 0.894 0.9648 0.803 0.9661 0.9173 0.8399 0.8323 0.9516
Peroxisomes 0.019 0.0003 0.0029 0.0118 0.0002 0.0001 0.0049 0.008 0.0022 0.0003 0.0209 0 0.0049 0 0.0033 0.0029 0.0101 0.0005
Punctate Nuclear 0.0222 0.004 0.0034 0.0018 0.0018 0.0038 0.0203 0.004 0.0019 0.0028 0.001 0.007 0.0113 0.0006 0.0043 0.0028 0.0379 0.0043
Vacuole 0.004 0.0009 0.0011 0.0022 0.01 0.0023 0.0139 0.0008 0.0002 0.0052 0.0002 0.0008 0.0038 0.0004 0.0018 0.0022 0.0024 0.0009
Vacuole Periphery 0.0017 0.0003 0.0021 0.0009 0.0099 0.0015 0.004 0.0001 0.0001 0.0029 0.0002 0.0002 0.0011 0.0001 0.0004 0.001 0.0028 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.5228 30.9617 44.3834 63.0377 32.4037 44.8434 51.2269 63.387 61.0364 48.9639
Translational Efficiency 0.7578 0.7827 0.797 0.5627 0.6678 0.8669 0.6607 0.5516 0.678 0.6425

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
167 165 1438 1350 1504 1961 964 282 1671 2126 2402 1632

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 656.28 952.10 1149.31 910.04 777.68 881.84 991.01 1005.17 765.55 887.29 1085.78 926.48
Standard Deviation 104.22 129.44 145.84 138.68 112.06 131.12 146.54 134.26 117.11 132.33 165.44 142.54
Intensity Change Log 2 0.536802 0.808385 0.471618 0.181340 0.349723 0.370191 0.354942 0.577822 0.417498

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000216 0.000644 0.000630 0.001004 0.000332 0.000292 0.000716 0.000860 0.000321 0.000319 0.000665 0.000979
Bud Neck 0.002472 0.004127 0.005161 0.011313 0.002951 0.004755 0.006712 0.013210 0.002903 0.004706 0.005784 0.011641
Bud Site 0.001100 0.001512 0.002541 0.024669 0.001003 0.001898 0.004446 0.014993 0.001012 0.001868 0.003306 0.022997
Cell Periphery 0.000269 0.000098 0.000093 0.000182 0.000117 0.000074 0.000206 0.000181 0.000132 0.000076 0.000138 0.000182
Cytoplasm 0.208467* 0.046959 0.084214 0.306390* 0.075469 0.063580 0.093103 0.199617* 0.088761 0.062290 0.087782 0.287940*
Cytoplasmic Foci 0.049676 0.007503 0.000825 0.011847 0.004501 0.006361 0.001246 0.003202 0.009015 0.006450 0.000994 0.010353
Eisosomes 0.000069 0.000033 0.000016 0.000038 0.000019 0.000018 0.000026 0.000036 0.000024 0.000019 0.000020 0.000038
Endoplasmic Reticulum 0.005358 0.013268 0.008992 0.005023 0.005466 0.007555 0.010076 0.012262 0.005455 0.007998 0.009427 0.006274
Endosome 0.002629 0.002519 0.000822 0.008275 0.000727 0.002360 0.001788 0.016097 0.000918 0.002372 0.001210 0.009627
Golgi 0.000225 0.008254 0.000076 0.004766 0.000303 0.000991 0.000353 0.010615 0.000295 0.001555 0.000187 0.005777
Lipid Particles 0.009265 0.003228 0.000475 0.001411 0.001372 0.001973 0.000565 0.001350 0.002161 0.002071 0.000511 0.001400
Mitochondria 0.001609 0.009938 0.001265 0.005941 0.003814 0.006414 0.002270 0.002449 0.003593 0.006688 0.001668 0.005338
Mitotic Spindle 0.000663 0.013323 0.001789 0.074703 0.004047 0.010661 0.009082 0.031263 0.003709 0.010867 0.004716 0.067197
None 0.017126 0.005425 0.003802 0.014998 0.008786 0.006386 0.011227 0.015034 0.009620 0.006311 0.006782 0.015004
Nuclear Periphery 0.005035 0.004012 0.004618 0.002971 0.003312 0.007617 0.003582 0.003991 0.003484 0.007337 0.004203 0.003147
Nuclear Periphery Foci 0.005996 0.003489 0.002258 0.002056 0.001959 0.003769 0.002610 0.003520 0.002362 0.003747 0.002399 0.002309
Nucleolus 0.008627 0.008315 0.000841 0.001855 0.005885 0.007940 0.002856 0.001158 0.006159 0.007969 0.001650 0.001735
Nucleus 0.667065* 0.859035* 0.865542* 0.458033* 0.876220* 0.854210* 0.830646* 0.604061* 0.855317* 0.854584* 0.851537* 0.483265*
Peroxisomes 0.001882 0.001390 0.000097 0.003135 0.000537 0.001635 0.000236 0.000505 0.000672 0.001616 0.000153 0.002680
Vacuole 0.011872 0.006427 0.015808 0.058280 0.002882 0.009648 0.015726 0.062749 0.003781 0.009398 0.015775 0.059052
Vacuole Periphery 0.000378 0.000500 0.000134 0.003109 0.000298 0.001862 0.002526 0.002845 0.000306 0.001756 0.001094 0.003064

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.87 -5.29 -3.81 -0.43 1.10 0.40 -7.40 -7.51 -9.03 -2.24 -0.22 -8.64 -5.66 -6.56 0.70
Bud Neck -2.64 -5.53 -6.85 -4.26 -2.81 -2.69 -6.67 -6.63 -5.23 -1.56 -3.02 -7.47 -7.94 -5.95 -2.58
Bud Site -0.96 -2.70 -6.62 -6.22 -5.29 -2.14 -3.21 -5.33 -4.42 -2.57 -2.25 -4.60 -7.69 -6.78 -5.56
Cell Periphery 1.61 1.54 1.37 -1.63 -2.35 3.50 -2.93 -2.98 -7.30 0.45 3.70 -0.31 -0.28 -8.84 0.08
Cytoplasm 8.34 7.91 -0.27 -14.94 -19.94 2.16 0.08 -4.16 -5.29 -4.12 5.06 3.33 -15.41 -21.09 -19.95
Cytoplasmic Foci 4.84 5.81 5.17 0.58 -7.80 -2.00 5.46 4.19 5.87 -2.40 1.80 7.66 3.18 1.55 -7.91
Eisosomes 3.18 4.81 1.88 -3.17 -14.32 -0.03 -7.58 -10.35 -10.50 -6.27 1.92 -1.92 -13.03 -17.18 -14.15
Endoplasmic Reticulum -2.66 -3.97 -1.44 2.22 5.14 -3.29 -9.52 -6.86 -5.17 -1.17 -4.01 -10.01 -5.08 -0.63 5.37
Endosome 0.20 2.24 -1.09 -1.21 -5.31 -4.54 -3.04 -2.54 -1.35 -1.94 -4.21 -2.20 -5.93 -2.35 -5.19
Golgi -1.54 1.69 -2.22 1.29 -2.51 -2.16 0.40 -1.13 -0.63 -1.18 -2.55 1.01 -2.33 0.03 -2.71
Lipid Particles 2.15 4.59 4.31 1.83 -3.45 -1.59 3.20 -0.87 1.02 -5.52 0.11 5.07 2.90 2.57 -5.70
Mitochondria -1.66 0.39 -1.94 1.39 -2.28 -1.85 1.64 2.14 4.57 1.33 -2.30 2.07 0.89 3.51 -1.80
Mitotic Spindle -1.68 -3.63 -11.55 -4.37 -10.73 -3.21 -2.91 -3.86 -3.03 -2.90 -3.64 -2.15 -11.22 -9.10 -10.50
None 2.54 3.10 1.60 -3.12 -6.96 2.24 0.47 -0.83 -2.52 -1.14 3.07 4.48 -0.30 -3.55 -5.14
Nuclear Periphery 0.98 -1.11 -1.19 -3.04 -0.08 -6.13 -3.77 -5.13 1.53 -2.11 -5.90 -4.93 -5.55 1.53 -0.99
Nuclear Periphery Foci 0.99 1.37 1.52 0.99 0.95 -2.15 -0.82 -1.74 0.67 -0.83 -1.49 -0.39 -0.06 1.78 0.51
Nucleolus 0.08 5.10 4.89 3.37 -1.57 -2.15 5.42 8.86 7.75 3.45 -2.00 8.94 9.72 8.11 1.44
Nucleus -6.63 -8.56 4.89 14.83 31.42 3.24 5.00 13.11 11.71 10.44 0.49 1.31 30.48 30.63 32.55
Peroxisomes 0.52 2.49 1.18 0.82 -2.39 -2.80 3.71 2.93 3.58 -0.50 -2.58 4.95 -0.51 1.79 -2.34
Vacuole 1.03 -3.85 -16.01 -18.99 -15.74 -5.35 -10.78 -11.01 -10.30 -8.76 -4.65 -15.01 -24.63 -22.68 -18.39
Vacuole Periphery -0.64 2.11 -2.26 -1.89 -3.32 -3.18 -1.31 -1.03 2.12 0.87 -3.19 -1.02 -2.87 1.54 -0.77