Standard name
Description Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0 0.08 0.09 0.08 0.16 0.1 0.11 0.11 0.09 0.11 0.1 0.13 0.17 0.21 0.17 – 0.35 – 0 0.05 0.06 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0 0 0.2 0.36 0.08 0.4 0.38 0.45 0.63 0.68 0.69 0.69 0 0 0 – 0 – 0 0 0 0 0 0
Nucleus 0.69 0.56 0.59 0.56 0.39 0.46 0.56 0.58 0.44 0.44 0.42 0.36 0.42 0.49 0.61 – 0.25 – 0.77 0.76 0.62 0.76 0.71 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.46 0.23 0.23 0.45 0.28 0.2 0.16 0.17 0.12 0.13 0.17 0.48 0.32 0.21 – 0.45 – 0.11 0.09 0.19 0.11 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 – 0 – 0 0 1 0 0 0
Bud 0 1 3 0 2 1 4 7 3 2 9 16 0 0 0 – 0 – 4 5 7 11 14 9
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 1 0 2 1
Bud Site 0 0 0 0 0 1 3 17 6 3 9 12 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 12 15 24 15 44 30 38 56 25 37 31 59 40 63 56 – 7 – 11 10 20 5 8 7
Endoplasmic Reticulum 0 0 1 1 2 0 0 0 0 0 0 0 0 2 2 – 0 – 0 0 3 0 1 2
Endosome 5 1 10 1 6 1 5 4 0 1 0 0 1 1 2 – 0 – 5 5 2 1 6 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 1 0 0 0 1 4
Mitochondria 3 4 54 64 22 117 132 234 179 235 218 313 2 0 9 – 0 – 1 2 7 0 3 2
Nucleus 248 107 162 100 104 135 196 300 124 152 133 165 97 145 196 – 5 – 323 171 214 177 229 215
Nuclear Periphery 1 0 0 0 1 0 2 1 5 3 1 4 1 1 0 – 0 – 5 0 3 0 1 2
Nucleolus 1 0 0 1 1 0 0 5 0 1 0 1 1 1 2 – 0 – 12 3 5 4 7 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 – 0 – 7 4 5 2 4 4
Vac/Vac Membrane 139 88 63 41 122 82 69 82 47 41 41 77 110 95 66 – 9 – 44 21 66 26 39 55
Unique Cell Count 362 192 274 177 270 291 352 519 282 344 314 453 230 294 320 20 420 226 343 233 324 324
Labelled Cell Count 409 217 318 223 304 367 449 708 389 475 446 648 252 308 333 21 420 226 343 233 324 324


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.6 5.1 4.9 5.9 5.0 4.8 4.7 4.0 3.7 3.6 3.5 7.8 8.0 6.7 – 14.3 – 7.6 8.1 8.9
Std Deviation (1e-4) 1.2 1.3 0.9 1.0 1.3 0.8 1.1 1.3 1.1 1.0 1.0 1.1 1.6 1.7 1.5 – 3.8 – 1.4 1.7 1.7
Intensity Change (Log2) – – – -0.06 0.2 -0.04 -0.11 -0.14 -0.37 -0.46 -0.52 -0.54 0.61 0.64 0.37 – 1.47 – 0.56 0.66 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cytoplasm -0.1 2.7 0.6 0.8 0.9 0 0.8 0.5 1.8 2.9 4.2 3.1 – 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Endosome 0 -1.0 -2.8 0 0 0 0 0 0 -2.5 -2.9 -2.6 – 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Mitochondria 3.9 -3.9 5.3 0 0 0 0 0 0 -6.7 -8.0 -6.7 – 0 –
Nucleus -0.6 -4.8 -3.0 -0.9 -0.4 -3.6 -3.7 -4.1 -6.0 -3.8 -2.3 0.5 – -3.0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Vacuole 0 5.5 1.4 -1.0 -2.5 -1.9 -3.7 -3.1 -2.0 5.8 2.5 -0.7 – 0 –