Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0 0
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.13 0.27 0.35 0.36 0 0.05 0.08 0.05 0.13 0.14
Mitochondria 0.93 0.88 0.92 0.98 0.89 0.86 0.93 0.87 0.94 0.95 0.94 0.93 0.87 0.93 0.84 0.13 0 0.07 0.82 0.82 0.74 0.73 0.63 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.07 0 0 0 0.13 0.18 0.09 0.13 0.09 0.11 0.12 0.07 0.05 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0.08 0.05 0.06 0.14 0.1 0.11 0.11 0.13 0.13 0.09 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.12 0.07 0 0 0 0.05 0.06 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.15 0.05 0 0.05 0 0 0.06 0 0 0 0 0.12 0.06 0.08 0.19 0.23 0.23 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 2 1 0 0 1 0 2 4 0 2 10 16
Bud 2 3 1 5 6 4 12 4 10 10 3 6 3 4 6 2 2 4 0 0 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Bud Site 0 0 0 0 0 0 3 5 3 3 2 5 0 0 0 2 0 0
Cell Periphery 4 12 1 2 2 1 2 3 0 1 0 3 8 9 15 6 5 7 0 0 0 0 1 1
Cytoplasm 1 10 3 0 6 4 5 13 5 4 3 2 10 5 24 6 10 1 0 3 3 1 2 3
Endoplasmic Reticulum 0 1 1 1 0 3 1 1 1 0 4 1 4 3 3 2 2 1 1 0 0 0 1 3
Endosome 2 2 0 0 10 2 2 4 1 0 0 0 5 9 23 8 4 2 6 5 1 4 13 12
Golgi 4 25 0 0 6 0 0 0 0 0 0 0 22 25 72 19 27 20 9 19 6 6 44 55
Mitochondria 207 324 58 147 213 157 309 316 289 256 169 203 274 412 461 9 3 4 186 329 58 96 212 236
Nucleus 0 1 0 0 0 0 2 4 0 2 0 1 1 0 2 3 2 1 0 2 0 0 1 1
Nuclear Periphery 15 25 1 5 9 24 59 33 40 23 20 27 23 21 12 0 1 1 2 4 2 2 4 3
Nucleolus 17 15 5 8 14 26 35 39 34 35 23 19 10 8 7 4 4 5 0 2 1 2 4 1
Peroxisomes 0 4 1 1 9 4 3 9 3 1 0 2 5 17 19 8 9 4 6 8 2 7 19 18
SpindlePole 1 0 0 0 0 2 4 4 4 3 1 1 2 1 3 4 1 1 1 0 0 1 1 1
Vac/Vac Membrane 39 54 3 1 11 5 8 20 11 4 2 3 39 26 44 13 18 13 7 15 0 3 8 29
Unique Cell Count 223 369 63 150 240 182 334 363 306 269 180 218 315 445 546 70 77 56 228 400 79 133 336 396
Labelled Cell Count 292 477 74 170 286 232 445 455 401 342 227 273 408 541 691 86 89 64 228 400 79 133 336 396


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 10.9 7.2 8.6 9.4 7.7 7.5 7.9 7.0 7.1 7.0 7.0 9.5 9.5 9.3 10.4 10.5 10.3 9.5 11.0 10.8
Std Deviation (1e-4) 1.9 2.2 1.7 1.9 1.9 1.9 2.3 2.5 2.1 2.1 2.0 2.4 2.1 1.7 1.8 2.9 2.6 3.0 2.4 2.7 2.8
Intensity Change (Log2) 0.25 0.37 0.1 0.05 0.13 -0.05 -0.02 -0.05 -0.04 0.39 0.39 0.36 0.52 0.54 0.51 0.39 0.61 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 1.7 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.1 4.5 5.2 5.2
Mitochondria 2.1 -0.8 -1.2 0.1 -1.1 0.7 1.0 0.5 0.3 -1.1 0.1 -1.6 -9.1 -10.5 -9.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.6 3.3 2.0 2.6 0 2.3 2.5 0 0 0 0 0 0
Nucleolus 0 0 1.3 0.6 0.7 0.7 1.1 1.0 0.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 1.8 0 0 2.4 3.1 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.8482 5.5609 5.7722 4.759 4.7677 5.3097 5.3345 5.5864 5.631 5.3779 5.8939 5.5213 7.7387 7.2271 7.2078 7.4536 7.6614 7.2202
Actin 0.0248 0.0027 0.0067 0.0001 0.0002 0.0063 0.0202 0.0117 0.0051 0.0085 0.0022 0.0025 0.012 0.0041 0.0036 0.0027 0.0004 0.0019
Bud 0.0004 0.0007 0.002 0 0 0.0005 0.0004 0.0003 0.0001 0.0001 0 0.0001 0.0006 0.0039 0.0028 0.0022 0 0.0001
Bud Neck 0.0033 0.0002 0.0001 0 0 0.0002 0.0004 0 0.0001 0 0 0.0001 0.0006 0.0002 0.0003 0 0 0.0001
Bud Periphery 0.0006 0.0014 0.0043 0 0.0002 0.0011 0.0006 0.0004 0.0002 0.0001 0.0001 0.0002 0.0025 0.0033 0.0062 0.0196 0.0002 0.0004
Bud Site 0.0024 0.0009 0.0013 0 0 0.0046 0.0044 0.0005 0.0003 0.0003 0 0.0001 0.0026 0.0053 0.002 0.0004 0 0.0003
Cell Periphery 0.0002 0.0003 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001 0.0003 0.0004 0.0005 0.0002 0 0.0001
Cytoplasm 0.0005 0.0002 0.0011 0 0 0 0.0026 0 0.0002 0 0 0.0002 0.0044 0.002 0.0034 0.0001 0 0.0003
Cytoplasmic Foci 0.0097 0.002 0.0038 0 0 0.0009 0.0158 0.0013 0.0043 0.0008 0.0008 0.0049 0.0115 0.0115 0.0055 0.0008 0.0001 0.0039
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0006 0.0002 0.0002 0.0002 0.0002 0.0002 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0004 0.001 0.0004 0 0 0.0001 0.0013 0 0.0003 0 0 0.0003 0.0021 0.001 0.0008 0.0001 0 0.0002
Endosome 0.011 0.009 0.0151 0.0002 0.0001 0.0029 0.0333 0.0092 0.0127 0.0009 0.0069 0.0076 0.0192 0.0186 0.0093 0.0087 0.0002 0.0068
Golgi 0.0262 0.0163 0.0083 0.0003 0.0002 0.0087 0.0308 0.0168 0.0137 0.0023 0.0162 0.0202 0.0223 0.0426 0.0092 0.0101 0.0003 0.0104
Lipid Particles 0.0202 0.0067 0.0063 0.0002 0.0003 0.0045 0.0237 0.0009 0.0041 0.0017 0.0007 0.0075 0.0345 0.0082 0.0051 0.001 0.0004 0.0058
Mitochondria 0.8516 0.9442 0.9329 0.9983 0.998 0.9567 0.8239 0.9525 0.9478 0.982 0.9658 0.9476 0.8038 0.8531 0.8275 0.9488 0.9953 0.948
None 0.0003 0.0001 0.0009 0 0 0 0.0014 0 0.0001 0 0 0.0004 0.0367 0.0151 0.0831 0.0001 0 0.0075
Nuclear Periphery 0.0007 0.0014 0.0006 0 0 0.0001 0.0036 0 0.0004 0 0 0.0013 0.0104 0.0004 0.0032 0.0002 0 0.001
Nucleolus 0.0004 0.0001 0.0005 0 0 0.0001 0.0014 0 0 0 0 0.0001 0.002 0.0003 0.0021 0 0 0.0006
Nucleus 0.0127 0.0002 0.0008 0 0 0 0.0031 0 0.0001 0 0 0.0001 0.0021 0.0003 0.0022 0.0001 0 0.0004
Peroxisomes 0.0228 0.003 0.005 0.0004 0.0006 0.011 0.0158 0.004 0.0077 0.0019 0.0052 0.003 0.0174 0.0256 0.0184 0.0022 0.0026 0.0076
Punctate Nuclear 0.0003 0.0001 0.0015 0 0 0 0.0035 0 0.0002 0 0 0.0005 0.0079 0.0009 0.002 0 0 0.0016
Vacuole 0.0046 0.0036 0.0034 0.0001 0.0001 0.0004 0.0023 0.0006 0.0008 0.0003 0.0003 0.0005 0.0024 0.0011 0.0059 0.0007 0.0001 0.0007
Vacuole Periphery 0.0065 0.0059 0.0048 0.0002 0.0002 0.0016 0.0107 0.0013 0.0016 0.0008 0.0014 0.0026 0.0044 0.0017 0.0065 0.0019 0.0003 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.3343 22.4176 18.4832 39.8027 18.0633 38.9877 47.8514 54.3941 47.0263 51.8482
Translational Efficiency 1.0903 2.7401 3.0859 1.7649 3.4455 2.5245 1.8191 2.3234 2.1442 2.0493

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency