Standard name
Human Ortholog
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.05 0.06 0.06 0 0 0 0.05 0.06 0.07 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.95 0.95 0.91 0.86 0.84 0.82 0.75 0.72 0.61 0.66 0.99 0.98 0.99 0.87 0.88 0.88 0.97 0.9 0.85 0.95 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.06 0.05 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.1 0.09 0.17 0.17 0.44 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0 0 0.07 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 5 10 6 3 7 9 0 1 0 0 0 0 0 2 1 0 1 1
Bud 0 3 0 0 1 0 2 2 3 1 4 2 1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 10 10 17 15 6 7 12 12 14 16 11 13 13 32 30 28 0 0 0 0 0 0
Cytoplasm 313 330 169 209 257 206 234 243 267 189 150 148 306 396 410 434 338 351 308 319 152 139 282 301
Endoplasmic Reticulum 1 7 4 8 10 11 18 15 16 16 12 3 1 7 5 11 12 10 1 6 10 1 2 8
Endosome 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 4 0 1 1 5 1 0 1 2
Golgi 0 2 1 0 1 0 2 0 1 0 0 0 0 0 0 3 3 1 0 1 1 0 0 0
Mitochondria 1 2 0 0 4 18 28 28 61 45 109 68 0 0 0 8 2 4 1 3 1 1 1 2
Nucleus 4 0 1 0 2 0 3 3 1 3 1 0 1 2 2 16 7 10 1 2 0 0 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 5 2 1 9 7 5 10 19 14 8 14 1 0 1 36 36 35 2 5 2 1 2 3
Unique Cell Count 316 343 177 220 282 240 277 297 354 263 246 225 308 404 416 497 386 399 319 353 179 146 303 328
Labelled Cell Count 322 353 187 228 302 259 303 318 389 283 306 262 321 420 432 544 429 441 319 353 179 146 303 328


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.5 11.2 10.7 10.1 9.1 8.4 8.3 7.5 7.4 7.0 7.5 11.7 11.8 11.2 12.4 12.7 12.0 11.3 11.9 11.8
Std Deviation (1e-4) 1.4 2.0 1.5 1.3 1.5 1.6 1.3 1.6 1.4 1.4 1.3 1.4 1.5 1.6 1.3 2.2 2.2 2.3 2.0 2.2 1.9
Intensity Change (Log2) – – – -0.07 -0.15 -0.31 -0.41 -0.43 -0.58 -0.6 -0.67 -0.58 0.06 0.08 0.01 0.14 0.18 0.1 0.01 0.09 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 0.2 0.3 -2.0 -1.9 -1.2 -0.5 0 0.6 -1.1 -1.4 -1.5 0.4 0.9 0.6
Cytoplasm -0.2 -1.8 -3.2 -3.6 -4.3 -5.7 -6.2 -8.1 -7.2 2.9 1.7 2.3 -3.0 -2.9 -2.8
Endoplasmic Reticulum 0.8 0.8 1.3 2.1 1.5 1.3 1.9 1.4 0 0 0 0 0 0.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 4.4 4.2 5.9 5.8 10.3 8.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 2.4 2.3 0 2.6 0 0 0 3.0 3.6 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4546 6.1677 5.7663 4.5409 4.9681 5.8754 4.7676 7.442 6.4123 5.448 6.1523 6.5614 6.3785 9.3183 7.9126 7.7636 7.8266 8.0417
Actin 0.02 0.0007 0.0036 0.0967 0.0381 0.0105 0.0039 0.001 0.0038 0.0106 0.001 0.0032 0.0072 0.0001 0.011 0 0.0136 0.0013
Bud 0.0021 0.0012 0.0007 0.0022 0.0026 0.0005 0.0053 0.0076 0.0037 0.0099 0.0045 0.0023 0.0005 0.0002 0.0003 0.0003 0.0009 0.0002
Bud Neck 0.003 0.004 0.0005 0.0027 0.001 0.0022 0.0019 0.0002 0.001 0.0005 0.0007 0.003 0.0009 0.0002 0.0005 0.0005 0.0008 0.0007
Bud Periphery 0.0009 0.0005 0.0004 0.0027 0.0043 0.0007 0.0021 0.0018 0.0021 0.0062 0.0015 0.0011 0.0004 0 0.0002 0.0001 0.0003 0.0001
Bud Site 0.0049 0.0105 0.0009 0.005 0.0035 0.0024 0.0022 0.0051 0.0053 0.0018 0.0014 0.0022 0.002 0.0005 0.0079 0.0003 0.0005 0.0001
Cell Periphery 0.0004 0.0006 0.0003 0.0006 0.0003 0.0002 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.766 0.897 0.9003 0.6211 0.6757 0.8731 0.8094 0.9493 0.8946 0.7868 0.8451 0.8902 0.798 0.9788 0.9029 0.9262 0.9016 0.9447
Cytoplasmic Foci 0.0445 0.0108 0.0116 0.0454 0.0562 0.0125 0.0281 0.0095 0.0231 0.0297 0.023 0.0197 0.0284 0.0043 0.0087 0.0066 0.012 0.0078
Eisosomes 0.0001 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0215 0.0035 0.0091 0.0104 0.0142 0.0373 0.0394 0.0021 0.0049 0.0186 0.0034 0.0119 0.0546 0.0044 0.0162 0.0112 0.0164 0.0145
Endosome 0.0451 0.0167 0.0252 0.0842 0.069 0.036 0.0431 0.0088 0.0248 0.0548 0.0454 0.032 0.044 0.0056 0.0205 0.0235 0.0315 0.0123
Golgi 0.0156 0.0052 0.0047 0.043 0.0234 0.0055 0.0084 0.0018 0.004 0.0114 0.0088 0.0075 0.008 0.0005 0.005 0.0002 0.0041 0.0024
Lipid Particles 0.02 0.0002 0.0007 0.0067 0.0035 0.0006 0.0027 0.0004 0.0028 0.004 0.0003 0.0008 0.011 0 0.0039 0 0.002 0.0055
Mitochondria 0.0091 0.0016 0.0208 0.0332 0.0612 0.0033 0.0146 0.0024 0.0041 0.0258 0.0409 0.0109 0.0076 0.0003 0.0119 0.0001 0.0016 0.0019
None 0.0067 0.0342 0.0098 0.0136 0.0027 0.0022 0.0068 0.0039 0.0033 0.0018 0.014 0.0038 0.005 0.0009 0.0014 0.0007 0.0026 0.0015
Nuclear Periphery 0.0028 0.001 0.0016 0.0014 0.0025 0.0047 0.0087 0.0004 0.002 0.0017 0.0007 0.0007 0.0066 0.0004 0.0009 0.001 0.0018 0.001
Nucleolus 0.0053 0.0011 0 0.0033 0.0002 0.0002 0.0007 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0 0.0001 0 0.0001 0.0001
Nucleus 0.0035 0.0023 0.0013 0.0036 0.0018 0.0022 0.0053 0.0008 0.0048 0.0014 0.0013 0.0013 0.0018 0.0008 0.0009 0.0014 0.0016 0.0008
Peroxisomes 0.0082 0.0002 0.0003 0.0064 0.0102 0.0004 0.0033 0.001 0.0062 0.0163 0.0007 0.0009 0.0053 0 0.0022 0 0.0008 0.002
Punctate Nuclear 0.0128 0.0007 0.0005 0.0029 0.0118 0.001 0.001 0.0003 0.0009 0.0004 0.001 0.0034 0.0018 0 0.0002 0.0001 0.0015 0.0007
Vacuole 0.0061 0.0067 0.0064 0.0116 0.009 0.0031 0.0103 0.0027 0.007 0.0144 0.0049 0.0037 0.0121 0.0025 0.0041 0.0247 0.0052 0.0019
Vacuole Periphery 0.0015 0.0013 0.0011 0.0033 0.0087 0.0014 0.0025 0.0004 0.0012 0.0033 0.0012 0.0011 0.004 0.0003 0.0008 0.0028 0.0011 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7108 14.989 16.5044 19.7533 16.1295 15.677 16.9887 20.1278 17.3961 18.5765
Translational Efficiency 0.6332 0.569 0.5552 0.4613 0.4892 0.4925 0.5281 0.3844 0.5269 0.4838

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1794 1828 646 1440 1324 2437 1227 1437 3118 4265 1873 2877

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 932.71 903.05 1249.02 1180.64 997.82 1042.96 1094.11 1127.74 960.36 982.99 1147.54 1154.22
Standard Deviation 121.68 193.57 148.76 162.44 126.61 150.56 199.04 185.60 127.91 183.87 197.50 176.38
Intensity Change Log 2 — -0.046623 0.421296 0.340069 — 0.063832 0.132906 0.176583 — 0.011522 0.279440 0.257884

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000170 0.000101 0.000478 0.000647 0.000285 0.000242 0.000078 0.000560 0.000218 0.000182 0.000216 0.000603
Bud Neck 0.001174 0.002157 0.001272 0.002057 0.000981 0.001365 0.002398 0.001311 0.001092 0.001704 0.002009 0.001684
Bud Site 0.023353 0.014303 0.015619 0.024555 0.019903 0.016216 0.005848 0.023576 0.021888 0.015396 0.009218 0.024066
Cell Periphery 0.000229 0.000111 0.000124 0.000116 0.000377 0.000087 0.000317 0.000118 0.000292 0.000097 0.000250 0.000117
Cytoplasm 0.772633* 0.801037* 0.791376* 0.705130* 0.690175* 0.734694* 0.588667* 0.879526* 0.737619* 0.763129* 0.658582* 0.792237*
Cytoplasmic Foci 0.141760 0.073259 0.064379 0.049630 0.202841* 0.149491 0.053671 0.034272 0.167697 0.116818 0.057364 0.041959
Eisosomes 0.000017 0.000013 0.000010 0.000007 0.000018 0.000010 0.000075 0.000006 0.000017 0.000011 0.000053 0.000006
Endoplasmic Reticulum 0.003656 0.001578 0.001651 0.000321 0.007400 0.002279 0.001354 0.000116 0.005245 0.001978 0.001456 0.000219
Endosome 0.005480 0.010191 0.006501 0.010114 0.012542 0.011217 0.006826 0.002918 0.008478 0.010777 0.006713 0.006520
Golgi 0.013058 0.004326 0.000838 0.004611 0.012487 0.013510 0.000335 0.001570 0.012816 0.009573 0.000508 0.003092
Lipid Particles 0.000764 0.001257 0.001306 0.000933 0.001453 0.000821 0.001688 0.001009 0.001056 0.001008 0.001556 0.000971
Mitochondria 0.002192 0.000852 0.000036 0.001034 0.001487 0.001653 0.000144 0.000280 0.001893 0.001310 0.000107 0.000657
Mitotic Spindle 0.000252 0.000845 0.003162 0.024639 0.001348 0.002805 0.001933 0.003330 0.000717 0.001965 0.002357 0.013996
None 0.001104 0.007573 0.000806 0.000428 0.000720 0.000330 0.003815 0.000522 0.000941 0.003434 0.002777 0.000475
Nuclear Periphery 0.000018 0.000156 0.000118 0.000089 0.000052 0.000103 0.000281 0.000032 0.000032 0.000126 0.000225 0.000060
Nuclear Periphery Foci 0.000216 0.000881 0.004807 0.000644 0.000653 0.000494 0.000938 0.001075 0.000401 0.000660 0.002273 0.000859
Nucleolus 0.000088 0.000755 0.000057 0.000180 0.000221 0.000106 0.000310 0.000105 0.000144 0.000384 0.000223 0.000143
Nucleus 0.003928 0.018359 0.016864 0.006178 0.004553 0.003689 0.103497 0.006329 0.004193 0.009977 0.073617 0.006254
Peroxisomes 0.000447 0.000728 0.000380 0.001283 0.000541 0.000934 0.000452 0.000952 0.000487 0.000846 0.000427 0.001117
Vacuole 0.018035 0.056205 0.089710 0.163894 0.033764 0.052442 0.226495* 0.041458 0.024714 0.054055 0.179317 0.102740
Vacuole Periphery 0.011427 0.005314 0.000505 0.003508 0.008202 0.007512 0.000877 0.000937 0.010058 0.006570 0.000749 0.002224

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.81 -5.27 -2.71 -3.23 0.01 0.67 4.16 -1.91 -2.30 -3.86 0.92 -0.66 -3.41 -3.81 -3.19
Bud Neck -2.45 -2.49 -4.22 0.23 -2.41 -2.13 -5.46 -2.65 0.26 3.70 -3.03 -5.58 -4.79 0.09 1.65
Bud Site 6.12 3.72 1.09 -3.32 -2.06 2.71 10.88 -0.77 -2.62 -7.75 6.47 11.66 0.39 -4.12 -7.88
Cell Periphery 6.34 5.39 5.15 0.43 1.56 1.54 0.51 1.40 -3.25 4.03 2.42 0.76 2.28 -0.90 4.24
Cytoplasm -3.65 0.84 9.30 11.99 6.16 -5.23 8.76 -22.24 -19.81 -28.89 -4.44 10.73 -6.77 -2.79 -15.61
Cytoplasmic Foci 15.15 15.41 22.86 7.47 5.07 8.95 27.83 32.60 29.66 6.72 13.39 31.18 38.85 27.75 7.53
Eisosomes 1.67 3.12 6.16 3.03 5.02 4.47 -9.26 7.91 6.49 12.02 4.11 -8.58 9.73 5.60 12.17
Endoplasmic Reticulum 3.24 2.93 5.92 4.55 2.48 4.21 5.13 6.18 7.47 4.06 5.14 6.12 8.42 8.65 4.55
Endosome -5.95 -1.58 -3.16 1.96 -1.68 1.58 7.61 11.13 11.34 5.34 -4.23 4.12 5.20 8.44 1.85
Golgi 14.53 25.00 11.62 1.34 -3.59 -1.04 24.06 19.22 13.79 -3.39 5.28 34.84 20.49 11.57 -4.82
Lipid Particles -2.76 -4.29 -3.12 0.78 2.11 5.63 -0.58 2.17 -2.43 2.21 0.38 -3.58 -0.75 -1.05 2.98
Mitochondria 1.95 3.57 2.33 0.34 -4.13 -0.22 1.88 1.74 5.29 -1.21 1.15 3.88 2.99 3.74 -4.08
Mitotic Spindle -1.95 -3.30 -6.71 -6.41 -5.45 -1.80 -0.66 -1.50 0.03 -0.55 -2.95 -2.30 -6.89 -5.90 -5.21
None -4.61 1.63 3.00 5.18 2.17 1.33 -2.64 0.76 -2.03 3.00 -4.00 -2.30 2.73 5.01 3.21
Nuclear Periphery -4.96 -8.18 -6.23 1.87 2.59 -0.94 -5.29 1.04 1.28 5.93 -2.86 -7.46 -3.53 1.77 5.99
Nuclear Periphery Foci -5.67 -10.90 -7.90 1.38 9.83 0.47 -3.00 -2.80 -1.95 -0.46 -1.33 -11.56 -5.79 -1.41 8.36
Nucleolus -6.66 1.85 -4.93 5.68 -8.16 0.98 -0.51 1.02 0.03 6.54 -3.59 -1.18 -0.02 5.08 3.14
Nucleus -8.74 -12.92 -6.42 6.39 9.74 1.17 -17.40 -3.44 -8.10 16.96 -7.12 -18.40 -6.96 3.07 17.42
Peroxisomes -1.83 0.91 -4.11 -1.34 -4.46 -3.70 0.99 -2.52 0.36 -2.85 -3.88 0.91 -4.84 -0.86 -4.86
Vacuole -14.25 -13.20 -29.90 -21.66 -8.96 -6.99 -27.50 -7.30 -0.51 24.43 -16.03 -30.55 -28.47 -18.80 10.64
Vacuole Periphery 6.68 12.82 9.15 4.71 -4.53 0.94 12.19 11.35 13.45 0.08 5.63 16.82 13.67 12.51 -3.62
Standard name
Human Ortholog
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Oca5

Oca5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oca5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available