Standard name
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.05 0.06 0.06 0 0 0 0.05 0.06 0.07 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.95 0.95 0.91 0.86 0.84 0.82 0.75 0.72 0.61 0.66 0.99 0.98 0.99 0.87 0.88 0.88 0.97 0.9 0.85 0.95 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.06 0.05 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.1 0.09 0.17 0.17 0.44 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0 0 0.07 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 5 10 6 3 7 9 0 1 0 0 0 0 0 2 1 0 1 1
Bud 0 3 0 0 1 0 2 2 3 1 4 2 1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 10 10 17 15 6 7 12 12 14 16 11 13 13 32 30 28 0 0 0 0 0 0
Cytoplasm 313 330 169 209 257 206 234 243 267 189 150 148 306 396 410 434 338 351 308 319 152 139 282 301
Endoplasmic Reticulum 1 7 4 8 10 11 18 15 16 16 12 3 1 7 5 11 12 10 1 6 10 1 2 8
Endosome 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 4 0 1 1 5 1 0 1 2
Golgi 0 2 1 0 1 0 2 0 1 0 0 0 0 0 0 3 3 1 0 1 1 0 0 0
Mitochondria 1 2 0 0 4 18 28 28 61 45 109 68 0 0 0 8 2 4 1 3 1 1 1 2
Nucleus 4 0 1 0 2 0 3 3 1 3 1 0 1 2 2 16 7 10 1 2 0 0 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 5 2 1 9 7 5 10 19 14 8 14 1 0 1 36 36 35 2 5 2 1 2 3
Unique Cell Count 316 343 177 220 282 240 277 297 354 263 246 225 308 404 416 497 386 399 319 353 179 146 303 328
Labelled Cell Count 322 353 187 228 302 259 303 318 389 283 306 262 321 420 432 544 429 441 319 353 179 146 303 328


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.5 11.2 10.7 10.1 9.1 8.4 8.3 7.5 7.4 7.0 7.5 11.7 11.8 11.2 12.4 12.7 12.0 11.3 11.9 11.8
Std Deviation (1e-4) 1.4 2.0 1.5 1.3 1.5 1.6 1.3 1.6 1.4 1.4 1.3 1.4 1.5 1.6 1.3 2.2 2.2 2.3 2.0 2.2 1.9
Intensity Change (Log2) – – – -0.07 -0.15 -0.31 -0.41 -0.43 -0.58 -0.6 -0.67 -0.58 0.06 0.08 0.01 0.14 0.18 0.1 0.01 0.09 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 0.2 0.3 -2.0 -1.9 -1.2 -0.5 0 0.6 -1.1 -1.4 -1.5 0.4 0.9 0.6
Cytoplasm -0.2 -1.8 -3.2 -3.6 -4.3 -5.7 -6.2 -8.1 -7.2 2.9 1.7 2.3 -3.0 -2.9 -2.8
Endoplasmic Reticulum 0.8 0.8 1.3 2.1 1.5 1.3 1.9 1.4 0 0 0 0 0 0.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 4.4 4.2 5.9 5.8 10.3 8.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 2.4 2.3 0 2.6 0 0 0 3.0 3.6 3.5