Standard name
Description Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.13 0.07 0.15 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.99 0.99 0.95 – 0.88 0.92 0.81 0.85 0.97 0.99 1.0 0.91 0.95 0.96 0.98 0.97 0.92 0.99 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 – 0.13 0.07 0.3 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 – 1 1 1 0 0 0 0 2 1 0 0 0 0 0 0 0
Bud 0 0 1 0 1 0 7 – 3 6 7 10 1 0 0 0 1 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 1 0 – 1 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 11 7 9 8 9 10 – 0 1 3 2 0 6 6 36 20 24 0 0 0 0 0 0
Cytoplasm 189 187 133 213 311 348 488 – 184 321 300 393 60 254 301 248 267 152 185 181 135 65 188 188
Endoplasmic Reticulum 0 1 0 0 1 0 1 – 1 5 0 14 1 3 0 13 11 14 0 0 1 0 0 0
Endosome 0 0 1 0 0 1 3 – 3 1 2 0 0 0 0 4 2 1 0 0 1 0 0 0
Golgi 0 0 0 0 2 0 0 – 0 0 0 0 0 0 0 3 4 2 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 10 35 – 28 23 110 76 0 1 0 4 2 1 0 1 2 0 1 1
Nucleus 0 1 2 2 2 1 1 – 3 3 2 4 1 1 0 4 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 3 0 0 3 0 0 0 0 0 0 0 0 0 0 2 2
Vac/Vac Membrane 0 0 0 0 0 1 4 – 1 3 3 3 0 1 0 9 7 4 1 1 3 0 0 0
Unique Cell Count 189 188 135 213 314 353 516 208 350 369 463 62 257 301 273 281 158 189 186 148 66 202 202
Labelled Cell Count 190 200 144 224 325 371 554 228 369 429 506 63 266 307 323 317 198 189 186 148 66 202 202


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.9 6.4 7.3 7.4 5.8 5.5 – 4.7 4.7 4.2 4.5 6.8 7.8 6.9 9.7 10.1 10.3 5.7 6.6 6.6
Std Deviation (1e-4) 0.8 1.1 0.9 1.1 1.1 1.1 1.0 – 1.1 0.8 0.8 0.7 0.8 1.1 1.0 2.1 2.2 2.4 0.6 1.2 1.2
Intensity Change (Log2) – – – 0.19 0.21 -0.15 -0.22 – -0.44 -0.45 -0.62 -0.53 0.08 0.28 0.1 0.59 0.65 0.67 -0.17 0.03 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 0 0 0 – 0 0 0 0 0 0 0 2.5 0.7 2.8
Cytoplasm 1.8 0.5 0.1 -1.9 – -3.4 -2.7 -4.9 -4.3 -0.8 0.3 2.1 -2.9 -1.7 -1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3.5
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim101-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available