TheCellVision

YHL017W

ORF	YHL017W
Human Ortholog	TMEM87A TMEM87B
Description	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication

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Scale images

YHL017W

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0.06	0	0	0	0	0	0	0	0	0	0	0.14	0.18	0.1	–	0.06	0.06	0	0	0	0	0.05	0.06
Bud	0	0	0	0	0	0	0.06	0.06	0.09	0.08	0.13	0.09	0	0	0	–	0.08	0.06	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Cytoplasm	0	0.06	0	0.05	0.05	0	0	0	0	0	0	0	0	0.07	0.08	–	0.14	0.11	0	0	0	0	0	0
Endoplasmic Reticulum	0.05	0.11	0	0	0	0	0	0	0	0	0	0	0	0.07	0.05	–	0	0	0	0	0	0	0	0
Endosome	0.14	0	0.21	0.2	0.28	0.06	0.12	0.22	0.05	0	0	0	0.16	0.12	0.14	–	0.17	0.08	0.23	0.23	0.21	0.2	0.17	0.17
Golgi	0.12	0	0.07	0.07	0.06	0	0	0	0	0	0	0	0.29	0.48	0.44	–	0.28	0.39	0.33	0.29	0.43	0.45	0.51	0.52
Mitochondria	0.8	0.83	0.75	0.77	0.61	0.87	0.8	0.62	0.86	0.89	0.91	0.87	0.38	0.24	0.18	–	0	0	0.18	0.21	0.06	0.12	0.11	0.09
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0.06	0	0	0.05	0	0	0	0	0	–	0	0.08	0	0	0	0	0	0
Peroxisomes	0	0	0.18	0.13	0.23	0.12	0.12	0.21	0.12	0.08	0.05	0.09	0.06	0	0	–	0.14	0.08	0.15	0	0.24	0.15	0.13	0.14
SpindlePole	0	0	0.07	0	0.08	0	0.09	0.16	0.09	0.07	0.06	0.09	0.16	0.08	0.09	–	0	0.06	0	0	0	0	0	0
Vac/Vac Membrane	0.08	0.11	0	0	0	0	0	0	0	0	0	0	0.08	0.06	0.15	–	0.06	0.08	0	0.12	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	2	2	3	6	6	1	1	3	4	2	1	2	65	70	36	–	4	2	1	0	18	22	44	49
Bud	2	0	22	17	22	26	63	58	74	84	80	76	14	6	10	–	6	2	0	0	0	0	0	1
Bud Neck	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	–	0	0	0	0	0	0	0	1
Bud Site	0	0	0	0	5	10	8	9	24	28	14	28	0	0	0	–	0	0	–	–	–	–	–	–
Cell Periphery	0	0	4	4	6	2	2	8	7	2	5	3	3	5	1	–	3	1	0	0	0	0	0	0
Cytoplasm	2	2	12	24	38	21	22	31	22	13	16	24	19	27	27	–	10	4	0	1	1	1	1	0
Endoplasmic Reticulum	4	4	4	1	1	1	1	1	4	2	1	3	14	26	16	–	2	0	0	0	0	1	2	2
Endosome	12	0	114	101	218	44	119	203	40	30	7	33	75	48	49	–	12	3	24	16	122	101	155	147
Golgi	10	1	37	36	49	0	1	7	3	0	0	1	137	189	155	–	20	14	36	20	256	231	465	460
Mitochondria	67	29	414	396	471	638	820	575	727	926	568	737	182	94	65	–	3	1	19	15	34	60	99	80
Nucleus	0	1	0	0	1	1	2	8	4	2	6	2	2	2	0	–	0	0	1	0	0	0	0	0
Nuclear Periphery	0	0	1	3	1	3	5	4	5	11	3	1	3	1	0	–	2	0	0	0	0	1	1	0
Nucleolus	0	0	3	5	14	19	63	38	25	47	20	25	4	0	2	–	3	3	0	0	1	0	0	0
Peroxisomes	3	0	101	69	177	87	125	191	102	88	31	78	29	5	5	–	10	3	16	2	144	78	120	124
SpindlePole	2	0	37	13	60	29	90	147	76	70	40	76	75	32	31	–	3	2	2	2	2	4	6	3
Vac/Vac Membrane	7	4	13	11	10	12	29	24	17	14	12	10	39	25	52	–	4	3	3	8	2	4	6	8
Unique Cell Count	84	35	551	516	770	733	1020	926	847	1042	627	846	477	392	353		71	36	109	71	591	511	909	884
Labelled Cell Count	111	43	765	686	1079	894	1351	1307	1134	1320	804	1099	661	530	449		82	38	109	71	591	511	909	884

Yeast GFP Assignment

Late Golgi

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	5.8	5.1	6.1	7.0	6.8	5.3	4.8	5.5	4.7	4.4	4.5	4.4	8.0	8.0	7.6	–	7.6	9.4	8.0	8.1	8.1
Std Deviation (1e^-4)	1.0	0.7	1.0	1.4	1.8	1.4	1.3	1.4	1.4	1.1	1.3	1.2	0.8	0.9	0.9	–	1.6	1.7	1.2	1.2	1.3
Intensity Change (Log₂)	–	–	–	0.2	0.18	-0.19	-0.35	-0.15	-0.36	-0.45	-0.44	-0.47	0.4	0.4	0.33	–	0.33	0.63	0.41	0.41	0.41

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	0	0	0	8.4	9.8	7.0	–	0	0
Bud	-0.6	-1.1	-0.4	0	1.9	0	0	0	0	-0.9	-2.2	-0.9	–	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0
Cytoplasm	2.2	2.6	0.8	0	1.3	0.5	-1.4	0.4	0.8	1.7	3.6	3.9	–	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	2.7	5.1	3.8	–	0	0
Endosome	-0.5	3.1	-7.9	0	0.6	0	0	0	0	-2.1	-3.4	-2.6	–	-0.7	-1.8
Golgi	0.2	-0.3	-7.1	0	-6.5	0	0	0	0	9.4	14.7	13.3	–	5.9	0
Mitochondria	0.6	-5.3	5.5	0	-5.2	0	0	0	0	-12.0	-15.5	-16.7	–	-12.0	-9.2
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0
Nucleolus	0	2.0	2.8	0	4.0	0	0	0	0	0	0	0	–	0	0
Peroxisomes	-2.2	2.0	-3.2	0	1.1	0	0	0	0	-5.9	-8.2	-7.7	–	-0.9	-1.5
SpindlePole	-3.2	0.7	-2.2	0	5.2	0	0	0	0	4.6	0.8	1.1	–	0	0
Vacuole	-0.3	-1.5	-0.9	0.6	0.3	-0.4	-1.5	-0.5	-1.7	4.2	3.1	7.0	–	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	3.0413	4.0406	3.7971	3.3322	4.1907	3.6194	1.8111	2.9329	2.6332	2.2057	1.8279	2.5355	1.5496	2.1145	2.0495	2.3529	1.6308	1.7912
Actin	0.0294	0.0136	0.0103	0.0086	0.0009	0.014	0.0701	0.017	0.0393	0.0104	0.0108	0.0281	0.0119	0.0068	0.0189	0.0213	0.0003	0.021
Bud	0.0002	0.0003	0.0003	0.0001	0	0.0009	0.0004	0.0007	0.0002	0.0002	0.0001	0.0001	0.0002	0.0002	0.0001	0.0002	0.0001	0.0001
Bud Neck	0.0005	0.0004	0.0005	0.0004	0.0002	0.0009	0.0008	0.001	0.0005	0.0006	0.0003	0.0009	0.0007	0.0005	0.001	0.0005	0.0002	0.0006
Bud Periphery	0.0002	0.0002	0.0002	0.0001	0	0.0009	0.0005	0.001	0.0001	0.0002	0.0001	0.0001	0.0002	0.0001	0.0001	0.0002	0	0.0001
Bud Site	0.0006	0.0011	0.0007	0.0004	0.0001	0.0007	0.0022	0.0189	0.0005	0.0003	0.0002	0.0003	0.0029	0.0008	0.0004	0.001	0.0001	0.0002
Cell Periphery	0.0001	0	0.0001	0	0	0.0002	0.0001	0.0005	0.0001	0	0	0	0.0002	0.0001	0.0001	0.0001	0	0.0001
Cytoplasm	0.0012	0.0067	0.0041	0.0009	0.0008	0.0037	0.0019	0.0093	0.0011	0.0008	0.0007	0.0009	0.0017	0.0039	0.0038	0.0013	0.0007	0.001
Cytoplasmic Foci	0.1535	0.1884	0.1513	0.1498	0.0954	0.1074	0.1343	0.1617	0.1139	0.1464	0.1018	0.1231	0.1405	0.1388	0.1019	0.0928	0.1147	0.0943
Eisosomes	0.0001	0	0	0	0	0.0001	0.0002	0.0004	0.0016	0	0	0.0001	0.0001	0.0001	0.0002	0.0001	0	0.0001
Endoplasmic Reticulum	0.0004	0.0003	0.0006	0.0002	0.0003	0.0007	0.0017	0.0005	0.0005	0.0002	0.0002	0.0002	0.0008	0.0007	0.0008	0.0027	0.0003	0.0004
Endosome	0.2488	0.2469	0.2457	0.2218	0.3318	0.1815	0.2094	0.1663	0.2086	0.2377	0.1583	0.1233	0.2118	0.2036	0.19	0.1904	0.2977	0.1752
Golgi	0.38	0.4115	0.4682	0.4281	0.4098	0.4758	0.3814	0.511	0.5334	0.4861	0.5066	0.5393	0.3873	0.4832	0.5455	0.4587	0.4812	0.5442
Lipid Particles	0.045	0.0315	0.0197	0.0482	0.0602	0.0353	0.0839	0.0118	0.0221	0.0502	0.0527	0.0475	0.1242	0.0526	0.037	0.0347	0.0844	0.0515
Mitochondria	0.0412	0.0163	0.0352	0.0323	0.0272	0.0669	0.0151	0.0386	0.0148	0.0077	0.0554	0.0247	0.0297	0.0458	0.0262	0.057	0.0055	0.0315
None	0.0002	0.0002	0.0004	0.0001	0.0001	0.0086	0.001	0.0019	0.0037	0.0001	0.0001	0.0001	0.0005	0.0002	0.0113	0.0003	0	0.0001
Nuclear Periphery	0.0001	0.0001	0.0004	0.0001	0.0001	0.0023	0.0016	0.0001	0.0009	0.0001	0.0001	0.0001	0.0008	0.0001	0.0005	0.0042	0.0001	0.0001
Nucleolus	0.0001	0.0001	0.0001	0.0001	0	0.0003	0.0013	0.0001	0.0003	0	0	0	0.0012	0.0003	0.0001	0.0001	0	0
Nucleus	0.0001	0.0001	0.0001	0	0	0.0005	0.0008	0.0001	0.0002	0	0	0	0.0005	0.0001	0.0003	0.0004	0	0
Peroxisomes	0.0945	0.0786	0.0579	0.1066	0.0685	0.0931	0.0882	0.0557	0.0549	0.0566	0.1106	0.1095	0.0728	0.0573	0.0574	0.1303	0.0113	0.0763
Punctate Nuclear	0.0005	0.0007	0.0004	0.0003	0.0001	0.0027	0.0017	0.0002	0.0008	0.0002	0.0001	0.0003	0.0014	0.0004	0.0008	0.0014	0.0001	0.0002
Vacuole	0.0017	0.0018	0.0021	0.0008	0.0016	0.0017	0.0019	0.0018	0.0011	0.001	0.0009	0.0005	0.0064	0.0024	0.0013	0.001	0.0015	0.0012
Vacuole Periphery	0.0018	0.0012	0.0018	0.001	0.0027	0.0019	0.0015	0.0011	0.0015	0.0013	0.001	0.0008	0.0042	0.0018	0.0022	0.0014	0.0019	0.0017

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	25.8772	16.0386	18.7675	23.1244	19.6832	18.6213	20.2708	20.6274	21.3149	28.1022
Translational Efficiency	0.5479	0.7599	0.6672	0.465	0.6166	0.8273	0.6095	0.4476	0.5911	0.5062

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
87	928	2018	178	644	1319	1616	515	731	2247	3634	693

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	1041.52	818.28	929.56	899.82	715.04	806.38	849.74	903.86	753.90	811.29	894.06	902.82
Standard Deviation	121.51	124.63	113.21	128.85	127.07	98.89	134.50	130.30	164.79	110.41	129.36	129.94
Intensity Change Log ₂	—	-0.348024	-0.164071	-0.210982	—	0.173436	0.248998	0.338075	—	-0.112615	0.018557	0.038191

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.004827	0.004330	0.012077	0.032138	0.001855	0.002668	0.049171	0.010300	0.002209	0.003354	0.028572	0.015909
Bud Neck	0.004904	0.000464	0.000282	0.002114	0.002696	0.000147	0.000377	0.002459	0.002959	0.000278	0.000325	0.002371
Bud Site	0.015907	0.002497	0.004368	0.016594	0.002570	0.000925	0.005981	0.012025	0.004157	0.001574	0.005085	0.013198
Cell Periphery	0.000389	0.000066	0.000052	0.000082	0.000144	0.000054	0.000098	0.000044	0.000173	0.000059	0.000073	0.000053
Cytoplasm	0.013679	0.002171	0.001513	0.007784	0.003177	0.000727	0.002569	0.009768	0.004427	0.001323	0.001983	0.009258
Cytoplasmic Foci	0.302752*	0.256922*	0.307512*	0.335798*	0.182009*	0.213763*	0.238186*	0.315446*	0.196380*	0.231587*	0.276683*	0.320673*
Eisosomes	0.000067	0.000051	0.000061	0.000096	0.000087	0.000064	0.000153	0.000062	0.000084	0.000059	0.000102	0.000070
Endoplasmic Reticulum	0.002618	0.000520	0.000420	0.001041	0.000261	0.000172	0.000434	0.000216	0.000542	0.000316	0.000426	0.000428
Endosome	0.296353*	0.178219	0.218429*	0.245338*	0.234389*	0.196623	0.280623*	0.247920*	0.241764*	0.189022	0.246086*	0.247257*
Golgi	0.170726*	0.484836*	0.400993*	0.151967*	0.432966*	0.515206*	0.359020*	0.216250*	0.401756*	0.502663*	0.382328*	0.199739*
Lipid Particles	0.009635	0.010929	0.008820	0.011179	0.037629	0.010006	0.009691	0.006843	0.034297	0.010387	0.009207	0.007957
Mitochondria	0.014069	0.027996	0.008955	0.002767	0.057937	0.031346	0.017077	0.005573	0.052716	0.029962	0.012566	0.004852
Mitotic Spindle	0.039855	0.006573	0.015505	0.065372	0.003673	0.002730	0.005028	0.048277	0.007979	0.004317	0.010846	0.052668
None	0.000059	0.000061	0.000015	0.000025	0.001276	0.000110	0.000030	0.000030	0.001131	0.000090	0.000022	0.000029
Nuclear Periphery	0.000132	0.000029	0.000056	0.000061	0.000061	0.000027	0.000026	0.000064	0.000070	0.000028	0.000043	0.000063
Nuclear Periphery Foci	0.000994	0.000566	0.000778	0.001163	0.000632	0.000294	0.000208	0.000475	0.000675	0.000406	0.000525	0.000652
Nucleolus	0.002095	0.000062	0.000015	0.000251	0.000507	0.000119	0.000023	0.000129	0.000696	0.000095	0.000019	0.000160
Nucleus	0.000829	0.000086	0.000027	0.000526	0.000333	0.000356	0.000025	0.000357	0.000392	0.000244	0.000026	0.000400
Peroxisomes	0.066176	0.016649	0.009459	0.092761	0.030951	0.019374	0.023899	0.087713	0.035143	0.018248	0.015881	0.089010
Vacuole	0.043451	0.003590	0.007596	0.031327	0.003034	0.002376	0.005321	0.030987	0.007844	0.002877	0.006584	0.031074
Vacuole Periphery	0.010484	0.003384	0.003067	0.001615	0.003813	0.002914	0.002060	0.005063	0.004607	0.003108	0.002619	0.004177

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	0.23	-3.00	-3.27	-3.39	-2.42	-0.98	-11.96	-3.55	-3.22	8.67	-1.27	-13.14	-4.94	-4.49	4.00
Bud Neck	2.70	2.81	1.62	-4.94	-5.71	2.19	1.98	-0.06	-4.73	-4.29	2.59	2.55	0.30	-5.59	-5.49
Bud Site	2.79	2.42	-0.10	-5.12	-4.47	2.54	-4.09	-5.06	-6.13	-3.37	3.08	-0.93	-5.37	-7.60	-5.34
Cell Periphery	1.47	1.53	1.37	-1.00	-1.92	2.69	1.36	2.97	1.39	7.09	2.90	2.55	2.99	0.47	2.72
Cytoplasm	1.81	1.92	0.75	-2.76	-3.16	2.51	0.56	-4.06	-5.72	-4.76	2.63	2.12	-3.48	-6.15	-5.81
Cytoplasmic Foci	2.09	-0.50	-1.51	-5.22	-1.80	-4.58	-8.00	-13.17	-10.82	-8.05	-5.53	-12.78	-13.59	-11.14	-5.34
Eisosomes	1.51	0.69	-1.76	-3.12	-2.57	2.44	-4.65	2.48	0.03	6.67	3.39	-1.97	1.23	-2.15	3.44
Endoplasmic Reticulum	1.22	1.29	0.64	-1.59	-1.96	1.08	-2.19	-0.14	-1.57	2.68	0.94	0.54	-0.15	-1.45	-1.06
Endosome	4.60	3.01	1.80	-3.92	-1.43	4.06	-4.93	-0.70	-4.43	3.57	6.18	-0.61	-0.11	-6.13	0.44
Golgi	-15.09	-11.52	0.82	22.88	18.55*	-7.08	6.54	16.06	26.71	13.60	-9.49	1.80	16.60	33.35	21.55*
Lipid Particles	-0.18	0.42	-0.32	-0.21	-0.97	5.40	5.56	5.93	1.27	1.24	5.35	5.71	5.74	1.15	0.55
Mitochondria	-2.10	0.78	1.80	7.79	4.98	4.41	7.10	9.13	8.83	4.97	4.28	8.00	9.42	11.02	5.11
Mitotic Spindle	2.67	1.99	-1.43	-4.69	-4.00	1.00	-1.38	-7.06	-7.24	-6.80	2.13	-1.32	-7.51	-8.50	-7.28
None	-0.06	1.51	0.64	0.77	-1.40	1.35	1.45	1.45	0.84	-0.12	1.37	1.47	1.45	1.00	-1.16
Nuclear Periphery	2.62	2.04	1.65	-3.33	-1.19	2.01	2.19	-0.90	-4.30	-5.01	2.80	1.89	-0.28	-5.29	-3.72
Nuclear Periphery Foci	1.60	1.05	-0.96	-1.34	-1.22	1.24	1.59	0.15	-1.76	-2.75	1.20	0.71	-0.92	-1.70	-1.41
Nucleolus	1.11	1.13	0.87	-1.49	-1.64	2.19	3.06	1.80	-0.66	-1.87	2.29	2.60	1.54	-1.63	-2.58
Nucleus	1.67	1.79	-0.42	-1.54	-1.62	-0.08	2.86	-1.16	-0.58	-3.07	0.71	3.42	-1.34	-1.67	-3.06
Peroxisomes	3.84	4.43	-1.44	-5.95	-6.58	3.15	1.98	-6.99	-8.86	-8.32	4.91	5.86	-7.47	-10.78	-11.29
Vacuole	3.47	3.17	1.18	-5.85	-5.05	1.78	-3.52	-7.35	-7.48	-6.89	3.34	1.20	-6.91	-8.93	-7.99
Vacuole Periphery	1.36	1.43	1.73	3.21	4.21	1.47	2.96	-0.61	-1.14	-1.64	1.80	2.48	0.34	-0.72	-1.11