Standard name
Human Ortholog
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.07 0.07 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.06 0.07 0.05 0.06 0.08 0.06 0.07 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.95 0.94 0.9 0.74 0.7 0.65 0.66 0.65 0.61 0.63 0.98 0.98 0.97 0.94 0.94 0.96 0.96 0.93 0.89 0.91 0.92 0.92
Endoplasmic Reticulum 0 0 0.05 0.05 0.07 0.11 0.06 0.16 0.08 0.11 0.06 0.11 0 0 0 0 0.05 0 0 0 0.05 0.05 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.15 0.1 0.24 0.18 0.33 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.08 0.07 0 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 4 10 19 13 14 10 13 0 0 0 0 1 2 0 0 0 0 1 0
Bud 0 0 0 0 0 0 10 2 2 1 7 1 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 4 4 8 10 14 18 15 11 19 12 14 2 9 12 23 17 20 0 0 0 1 0 0
Cytoplasm 122 262 142 184 251 161 193 189 127 147 124 122 292 352 384 348 375 385 121 256 144 249 353 301
Endoplasmic Reticulum 1 9 7 10 21 25 16 45 15 26 12 21 6 11 14 10 20 16 0 8 8 13 12 17
Endosome 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 1 0 1 4 4 5 2 2 0 4 0 2 0 0 6 1 0 0 1 0 0 0
Mitochondria 0 1 0 0 0 21 41 28 46 40 67 37 0 0 0 2 3 9 0 0 2 4 2 1
Nucleus 0 1 0 0 1 1 3 3 0 2 1 1 0 1 0 0 1 0 1 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 1 8 14 22 21 7 13 5 5 2 1 1 17 11 5 1 3 2 0 4 1
Unique Cell Count 122 271 150 196 280 219 274 289 191 227 204 193 297 361 397 371 398 403 127 275 162 273 383 327
Labelled Cell Count 125 277 156 203 295 244 318 327 224 264 239 219 302 376 411 400 434 441 127 275 162 273 383 327


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.8 29.4 19.0 19.5 16.7 14.3 12.7 13.6 12.1 12.5 11.7 12.4 23.6 23.4 23.2 29.1 30.1 29.6 26.8 26.7 28.4
Std Deviation (1e-4) 3.7 5.2 3.9 3.8 2.6 2.7 2.4 2.4 2.4 2.4 2.2 2.5 5.1 3.8 4.0 8.1 7.1 7.1 5.1 5.4 5.2
Intensity Change (Log2) 0.03 -0.19 -0.42 -0.59 -0.49 -0.65 -0.6 -0.7 -0.62 0.31 0.3 0.29 0.61 0.66 0.64 0.49 0.49 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.2 3.3 3.1 0 3.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0 1.6 1.7 1.2 1.4 2.3 1.4 1.9 0 0 0 1.6 0.9 1.2
Cytoplasm -0.3 -1.8 -5.2 -5.9 -6.8 -6.3 -6.7 -7.3 -6.9 2.2 1.6 1.1 -0.4 -0.2 0.4
Endoplasmic Reticulum 0.2 1.1 2.3 0.5 3.4 1.2 2.3 0.5 2.1 0 -0.9 -0.6 0 0.2 -0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 5.0 3.9 6.5 5.4 7.8 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.3 2.9 2.6 0 2.1 0 0 0 0 0 1.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.4019 22.268 21.1482 19.4442 19.3869 20.578 26.0221 27.5711 29.2443 26.6011 24.083 25.0616 26.7089 28.8167 28.6332 27.7767 27.3526 25.8378
Actin 0.0005 0.0005 0.0003 0 0.0006 0.0007 0.0003 0.0004 0.0002 0.0001 0.0001 0.0005 0.0004 0.0003 0.0009 0.0001 0.0002 0.0008
Bud 0.0004 0.0007 0.0003 0.0002 0.0006 0.0005 0.0003 0.0003 0.0004 0.0003 0.0004 0.0007 0.0003 0.0003 0.0003 0.0005 0.0003 0.0007
Bud Neck 0.0004 0.0004 0.0005 0.0004 0.0006 0.0011 0.0004 0.0009 0.0007 0.0012 0.0009 0.0018 0.0005 0.0006 0.0006 0.0008 0.0009 0.0015
Bud Periphery 0.0002 0.0003 0.0002 0.0001 0.0002 0.0007 0.0003 0.0002 0.0006 0.0005 0.0002 0.0008 0.0004 0.0004 0.0004 0.0002 0.0001 0.0008
Bud Site 0.0002 0.0016 0.0002 0.0001 0.0002 0.0001 0.0002 0.002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001
Cell Periphery 0.0006 0.0007 0.0005 0.0001 0.0002 0.0004 0.0011 0.0011 0.0009 0.0007 0.0002 0.0007 0.0015 0.0024 0.0019 0.0007 0.0002 0.0008
Cytoplasm 0.8757 0.9593 0.934 0.8386 0.8645 0.8742 0.9288 0.8964 0.9393 0.8829 0.8984 0.8758 0.8862 0.8951 0.9115 0.8714 0.9296 0.8739
Cytoplasmic Foci 0.0067 0.0038 0.0056 0.007 0.0143 0.0097 0.003 0.0081 0.0026 0.0092 0.0061 0.0061 0.0029 0.0029 0.0035 0.0036 0.006 0.0078
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0583 0.0157 0.0197 0.0229 0.0347 0.0469 0.0445 0.0642 0.0308 0.0405 0.0554 0.0724 0.0738 0.0683 0.0461 0.0379 0.0285 0.0745
Endosome 0.027 0.0061 0.0192 0.0391 0.0501 0.033 0.0064 0.0091 0.006 0.0193 0.0157 0.0148 0.0088 0.0066 0.0083 0.0152 0.0195 0.02
Golgi 0.0035 0.0007 0.0012 0.0009 0.004 0.005 0.0013 0.0027 0.0009 0.0015 0.0025 0.0039 0.0021 0.0017 0.002 0.0005 0.0014 0.0036
Lipid Particles 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.0004 0.0005 0.0002 0 0 0.0001 0.0002 0.0004 0.0006 0.0002 0.0001 0.0002
Mitochondria 0.0024 0.0013 0.0008 0.0439 0.0054 0.0146 0.0016 0.001 0.0006 0.0018 0.0015 0.0102 0.0021 0.0069 0.0045 0.0005 0.0008 0.0049
None 0.0008 0.0004 0.0003 0.0003 0.0004 0.0005 0.0005 0.0003 0.0003 0.0004 0.0003 0.0006 0.0004 0.0003 0.0003 0.0002 0.0003 0.0005
Nuclear Periphery 0.0047 0.001 0.0015 0.0044 0.0023 0.0016 0.0012 0.0012 0.001 0.0023 0.0014 0.001 0.0026 0.0018 0.0017 0.002 0.0015 0.0014
Nucleolus 0 0.0001 0 0 0 0 0 0.0014 0 0 0 0 0 0 0 0 0 0
Nucleus 0.0022 0.0015 0.0011 0.0016 0.0014 0.0012 0.0008 0.002 0.001 0.0016 0.0012 0.0009 0.0012 0.0012 0.001 0.0015 0.0011 0.0009
Peroxisomes 0 0 0 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0 0 0.0001
Punctate Nuclear 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0013 0 0 0 0 0 0 0 0 0 0
Vacuole 0.0139 0.005 0.0125 0.0341 0.0153 0.0069 0.008 0.0061 0.013 0.0338 0.0133 0.0075 0.0146 0.0096 0.0145 0.0606 0.0075 0.006
Vacuole Periphery 0.0019 0.0006 0.0018 0.006 0.0047 0.0027 0.001 0.0008 0.0012 0.0037 0.0022 0.0019 0.0017 0.0008 0.0013 0.0039 0.0018 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 127.7566 117.7915 127.6894 106.4399 111.4184 87.0325 95.797 75.4908 80.2706 101.1821
Translational Efficiency 1.376 1.6381 1.2205 1.4263 1.4384 1.6784 1.6309 1.6022 1.4857 1.4345

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
585 679 273 731 1992 2046 464 1324 2577 2725 737 2055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1658.08 1836.31 2274.38 2362.72 1605.07 1789.16 2220.29 2504.84 1617.10 1800.91 2240.33 2454.29
Standard Deviation 262.59 321.29 308.13 436.80 245.59 296.49 445.60 424.69 250.54 303.54 401.07 434.40
Intensity Change Log 2 0.147296 0.455960 0.510935 0.156646 0.468112 0.642082 0.151903 0.461950 0.576933

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000427 0.000268 0.000546 0.000371 0.000122 0.000109 0.000691 0.000379 0.000191 0.000149 0.000637 0.000376
Bud Neck 0.000626 0.000692 0.004838 0.002252 0.000162 0.000257 0.006361 0.001487 0.000267 0.000365 0.005797 0.001759
Bud Site 0.030852 0.017827 0.024507 0.017724 0.009393 0.009844 0.023419 0.016930 0.014264 0.011833 0.023822 0.017212
Cell Periphery 0.002547 0.003692 0.025409 0.025059 0.001271 0.000405 0.003347 0.016546 0.001561 0.001224 0.011519 0.019575
Cytoplasm 0.656314* 0.802686* 0.542324* 0.651945* 0.815694* 0.877580* 0.540227* 0.773124* 0.779513* 0.858918* 0.541004* 0.730018*
Cytoplasmic Foci 0.131376 0.046207 0.022954 0.008056 0.073306 0.039456 0.034045 0.008499 0.086489 0.041138 0.029937 0.008341
Eisosomes 0.000004 0.000004 0.000007 0.000005 0.000002 0.000001 0.000009 0.000004 0.000002 0.000002 0.000008 0.000004
Endoplasmic Reticulum 0.011474 0.001875 0.002134 0.001406 0.005260 0.001450 0.006607 0.000506 0.006671 0.001556 0.004950 0.000826
Endosome 0.010872 0.005162 0.008795 0.002478 0.003263 0.003110 0.013691 0.001659 0.004990 0.003621 0.011878 0.001950
Golgi 0.074374 0.056396 0.066671 0.038836 0.040662 0.023734 0.031251 0.020775 0.048315 0.031872 0.044371 0.027200
Lipid Particles 0.004680 0.009504 0.036673 0.027888 0.001810 0.001539 0.015972 0.026183 0.002461 0.003524 0.023640 0.026790
Mitochondria 0.000403 0.000255 0.000070 0.000055 0.000583 0.000229 0.000057 0.000172 0.000542 0.000236 0.000062 0.000131
Mitotic Spindle 0.003200 0.001816 0.004149 0.000853 0.001228 0.002298 0.003632 0.001561 0.001675 0.002178 0.003824 0.001309
None 0.000205 0.000439 0.000579 0.000488 0.000345 0.000270 0.000378 0.000319 0.000314 0.000312 0.000453 0.000379
Nuclear Periphery 0.000068 0.000045 0.000345 0.000182 0.000051 0.000035 0.000428 0.000130 0.000055 0.000038 0.000398 0.000149
Nuclear Periphery Foci 0.004476 0.004557 0.017472 0.008011 0.001923 0.001559 0.012889 0.004393 0.002502 0.002306 0.014586 0.005680
Nucleolus 0.000020 0.000252 0.000076 0.000116 0.000018 0.000026 0.000161 0.000113 0.000018 0.000082 0.000130 0.000114
Nucleus 0.000841 0.002805 0.004582 0.004762 0.000636 0.001594 0.013747 0.003550 0.000683 0.001896 0.010352 0.003981
Peroxisomes 0.000221 0.000462 0.000100 0.000179 0.000069 0.000114 0.000281 0.000173 0.000103 0.000201 0.000214 0.000175
Vacuole 0.021553 0.023152 0.204246* 0.180729 0.008493 0.019223 0.274130* 0.112023 0.011458 0.020202 0.248243* 0.136463
Vacuole Periphery 0.045469 0.021905 0.033523 0.028605 0.035711 0.017166 0.018676 0.011474 0.037926 0.018347 0.024176 0.017568

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.14 -1.89 0.62 -2.39 3.00 0.55 -6.55 -5.50 -5.77 3.60 1.75 -7.48 -5.41 -6.87 4.40
Bud Neck -0.65 -7.18 -8.18 -7.52 4.36 -3.29 -12.54 -13.21 -12.40 10.02 -2.81 -14.53 -15.57 -14.46 10.73
Bud Site 4.76 1.51 4.28 -0.27 1.97 -0.58 -7.82 -5.71 -5.37 3.79 2.51 -6.42 -2.79 -4.87 4.19
Cell Periphery -1.16 -4.77 -9.82 -8.64 -0.05 7.79 -4.57 -12.65 -13.45 -10.49 1.30 -5.40 -15.98 -16.00 -3.98
Cytoplasm -9.14 5.05 0.59 10.07 -4.68 -8.92 18.01 5.30 12.94 -14.32 -11.90 18.96 6.63 17.51 -14.14
Cytoplasmic Foci 12.34 16.43 19.75 12.50 7.15 11.12 10.80 24.25 17.00 11.46 15.81 18.46 30.85 21.25 13.34
Eisosomes 0.12 -3.80 -2.63 -1.08 2.17 3.41 -10.01 -4.56 -6.04 6.25 1.12 -10.93 -6.09 -5.34 6.44
Endoplasmic Reticulum 4.52 4.38 4.70 1.00 1.11 6.78 -0.43 9.07 4.50 3.65 7.86 1.91 9.08 3.30 3.82
Endosome 6.30 1.56 10.56 5.51 6.47 0.44 -9.61 6.07 5.06 11.10 4.31 -8.60 11.17 6.92 12.78
Golgi 3.16 1.44 7.34 3.92 4.38 7.72 3.81 8.53 1.69 2.93 7.52 1.99 9.83 2.70 5.22
Lipid Particles -2.43 -7.97 -10.32 -6.23 1.90 0.94 -9.92 -14.12 -14.22 -4.41 -1.98 -12.04 -17.81 -16.03 -1.38
Mitochondria 0.68 1.90 1.98 1.54 1.05 1.41 2.08 1.57 0.74 -1.48 1.53 2.42 2.02 1.75 -1.38
Mitotic Spindle 2.22 -1.23 4.02 2.81 4.41 -2.38 -4.53 -0.52 1.09 3.03 -1.38 -4.88 0.87 1.82 4.76
None -2.94 -1.44 -2.83 -0.34 0.31 2.32 -0.21 0.71 -1.54 0.51 0.05 -1.13 -1.40 -1.45 0.55
Nuclear Periphery 0.96 -7.54 -4.43 -8.63 5.00 0.83 -6.89 -2.34 -3.13 5.05 1.10 -9.21 -3.92 -5.42 6.36
Nuclear Periphery Foci -0.16 -7.51 -4.46 -4.56 5.35 1.54 -8.40 -8.15 -9.21 6.35 0.77 -11.65 -9.84 -10.44 8.33
Nucleolus -1.14 -10.52 -11.56 0.66 -3.84 -1.63 -5.18 -6.36 -6.13 1.76 -1.25 -6.30 -9.38 -0.64 1.05
Nucleus -1.86 -5.62 -8.23 -1.99 0.37 -11.15 -7.21 -11.71 -8.46 5.49 -4.49 -8.23 -14.26 -6.98 5.36
Peroxisomes -0.72 3.19 0.99 0.90 -1.61 -3.43 -7.58 -4.54 -2.25 3.54 -1.19 -5.51 -2.86 0.37 1.96
Vacuole -0.53 -13.72 -19.57 -19.16 1.45 -8.43 -21.03 -23.14 -20.29 11.90 -7.07 -25.15 -30.40 -27.75 10.68
Vacuole Periphery 5.06 2.63 3.82 -1.87 1.25 8.38 7.04 11.78 4.12 2.71 9.72 6.47 10.50 0.88 2.89