Standard name | |
---|---|
Human Ortholog | |
Description | Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 9 | 8 | 1 | 2 | – | 7 | 1 | 3 | 4 | 4 | 2 | 7 | 14 | 0 | 2 | 0 | 0 | 2 | 7 | 2 | 4 | 8 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | – | 1 | 1 | 4 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 8 | 0 | 0 | 0 | – | 3 | 1 | 0 | 2 | 3 | 4 | 14 | 29 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 4 | 15 | 10 | 17 | 10 | – | 54 | 11 | 8 | 15 | 20 | 7 | 23 | 35 | 5 | 16 | 6 | 0 | 0 | 2 | 0 | 0 | 4 |
Endoplasmic Reticulum | 45 | 65 | 79 | 15 | 3 | 7 | – | 32 | 17 | 18 | 20 | 25 | 123 | 374 | 79 | 0 | 3 | 7 | 76 | 81 | 457 | 306 | 410 | 530 |
Endosome | 0 | 0 | 3 | 4 | 10 | 6 | – | 3 | 4 | 9 | 8 | 8 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 4 | 15 |
Golgi | 3 | 0 | 36 | 14 | 6 | 1 | – | 23 | 6 | 7 | 1 | 12 | 12 | 30 | 19 | 0 | 12 | 16 | 0 | 0 | 1 | 3 | 5 | 6 |
Mitochondria | 0 | 1 | 9 | 12 | 7 | 7 | – | 13 | 10 | 23 | 19 | 18 | 0 | 0 | 0 | 2 | 13 | 12 | 0 | 0 | 3 | 4 | 5 | 11 |
Nucleus | 1 | 0 | 2 | 3 | 3 | 20 | – | 25 | 9 | 23 | 29 | 29 | 3 | 2 | 4 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 34 | 19 | 87 | 113 | 107 | 192 | – | 338 | 261 | 249 | 187 | 282 | 142 | 114 | 49 | 4 | 27 | 25 | 7 | 20 | 51 | 155 | 123 | 154 |
Nucleolus | 1 | 0 | 3 | 9 | 41 | 21 | – | 64 | 50 | 31 | 32 | 61 | 1 | 0 | 6 | 0 | 3 | 4 | 0 | 0 | 0 | 2 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 13 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 7 |
Vac/Vac Membrane | 10 | 19 | 163 | 149 | 228 | 205 | – | 355 | 203 | 316 | 178 | 349 | 72 | 34 | 149 | 6 | 63 | 34 | 0 | 3 | 6 | 25 | 34 | 86 |
Unique Cell Count | 64 | 90 | 349 | 303 | 400 | 420 | 787 | 468 | 598 | 421 | 681 | 283 | 454 | 336 | 16 | 119 | 96 | 87 | 111 | 543 | 511 | 598 | 845 | |
Labelled Cell Count | 94 | 113 | 416 | 338 | 440 | 474 | 918 | 575 | 691 | 500 | 814 | 366 | 598 | 391 | 19 | 143 | 108 | 87 | 111 | 543 | 511 | 598 | 845 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.8 | 14.1 | 21.1 | 21.2 | 20.2 | 16.5 | – | 17.2 | 15.9 | 16.0 | 15.8 | 16.4 | 17.5 | 18.6 | 18.5 | 12.8 | 18.8 | 22.7 | 20.6 | 21.0 | 21.8 |
Std Deviation (1e-4) | 2.9 | 2.7 | 4.3 | 4.4 | 4.5 | 3.5 | – | 5.2 | 3.9 | 3.7 | 4.4 | 4.1 | 2.9 | 3.2 | 3.6 | 3.2 | 4.7 | 5.6 | 3.2 | 3.7 | 4.0 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.06 | -0.35 | – | -0.29 | -0.41 | -0.4 | -0.41 | -0.36 | -0.26 | -0.18 | -0.19 | -0.72 | -0.16 | 0.11 | -0.03 | -0.01 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.7 | 0 | 0 | -1.0 | 1.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -0.8 | 0.7 | 3.7 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 0 | -1.5 | – | 1.7 | -1.6 | -2.9 | -0.5 | -1.1 | -1.2 | 0.5 | 3.1 | 0 | 3.5 | 0 |
Endoplasmic Reticulum | -6.4 | -9.6 | -9.2 | – | -9.7 | -8.3 | -9.6 | -7.4 | -9.6 | 5.6 | 16.9 | 0.3 | 0 | -5.0 | -3.4 |
Endosome | 0 | 1.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.7 | -5.2 | -6.5 | – | -5.2 | -5.8 | -6.5 | -6.5 | -6.2 | -2.9 | -1.9 | -2.2 | 0 | -0.1 | 1.7 |
Mitochondria | 1.0 | -0.8 | -0.9 | – | -1.0 | -0.4 | 1.0 | 1.4 | 0.1 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Nucleus | 0 | 0 | 3.5 | – | 2.7 | 0 | 3.0 | 4.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3.4 | 0.6 | 6.0 | – | 5.8 | 8.8 | 5.2 | 5.6 | 5.2 | 6.6 | 0.1 | -3.4 | 0 | -0.5 | 0.2 |
Nucleolus | 2.0 | 5.5 | 3.3 | – | 4.8 | 5.6 | 3.5 | 4.5 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 2.8 | 0.6 | – | -0.5 | -0.9 | 1.8 | -1.2 | 1.4 | -5.5 | -12.8 | -0.6 | -0.7 | 1.2 | -2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0095 | 0.0002 | 0.0041 | 0.0005 | 0.0119 | 0.0006 | 0.0279 | 0.0004 | 0.0154 | 0.0006 | 0.0188 | 0.0004 | 0.0145 | 0.0017 | 0.0027 | 0.0002 | 0.0004 | 0.0008 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 |
Bud Neck | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0001 | 0.0012 | 0.0001 |
Bud Periphery | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0013 | 0 | 0.001 | 0 | 0.0003 | 0.0001 | 0.0064 | 0 | 0.0041 | 0 |
Cell Periphery | 0.0004 | 0.0026 | 0.0012 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.002 | 0.0011 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0008 | 0.0003 | 0.0002 |
Cytoplasm | 0.004 | 0.0039 | 0.0058 | 0.0014 | 0.0005 | 0.001 | 0.0203 | 0.0004 | 0.001 | 0.0008 | 0.0007 | 0.0002 | 0.0167 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0049 | 0.0224 | 0.0173 | 0.0059 | 0.0041 | 0.006 | 0.0122 | 0.0778 | 0.0265 | 0.015 | 0.0116 | 0.0036 | 0.0003 | 0.0001 | 0.1909 | 0 | 0.0781 | 0.0001 |
Eisosomes | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.1102 | 0.3321 | 0.2091 | 0.4244 | 0.3367 | 0.2091 | 0.1057 | 0.333 | 0.2831 | 0.4548 | 0.425 | 0.1821 | 0.0172 | 0.1325 | 0.0001 | 0.944 | 0.0001 | 0.0073 |
Endosome | 0.0034 | 0.0006 | 0.0076 | 0.0011 | 0.0111 | 0.0026 | 0.0262 | 0.0009 | 0.002 | 0.001 | 0.0093 | 0.0005 | 0.0103 | 0.0024 | 0.0008 | 0.0016 | 0.0009 | 0.0004 |
Golgi | 0.0025 | 0.0003 | 0.0028 | 0.0003 | 0.009 | 0.0009 | 0.0065 | 0.0015 | 0.0017 | 0.0006 | 0.0074 | 0.0008 | 0.0003 | 0.0014 | 0.0037 | 0.0002 | 0.0023 | 0.001 |
Lipid Particles | 0.006 | 0.0098 | 0.0147 | 0.0082 | 0.0147 | 0.0143 | 0.032 | 0.0644 | 0.0595 | 0.0477 | 0.067 | 0.0155 | 0.0001 | 0.0025 | 0.099 | 0.0003 | 0.0658 | 0.0045 |
Mitochondria | 0.0013 | 0.0005 | 0.0023 | 0.0004 | 0.0039 | 0.0014 | 0.0019 | 0.0006 | 0.0004 | 0.0007 | 0.0029 | 0.0001 | 0.0098 | 0.003 | 0.0111 | 0.0001 | 0.0011 | 0.0008 |
None | 0.0052 | 0.0001 | 0.0014 | 0.0001 | 0.0004 | 0.0008 | 0.0093 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.004 | 0.0003 | 0.0014 | 0 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.7694 | 0.4806 | 0.517 | 0.4826 | 0.5348 | 0.6825 | 0.6859 | 0.3548 | 0.4364 | 0.4003 | 0.3225 | 0.7421 | 0.6467 | 0.7304 | 0.0011 | 0.0445 | 0.0009 | 0.9502 |
Nucleolus | 0.0092 | 0.0213 | 0.0534 | 0.0036 | 0.0025 | 0.0062 | 0.0015 | 0.0422 | 0.0432 | 0.0028 | 0.0301 | 0.0027 | 0.0008 | 0.0001 | 0.153 | 0 | 0.463 | 0.0015 |
Nucleus | 0.0199 | 0.0421 | 0.0381 | 0.0109 | 0.0468 | 0.0162 | 0.0142 | 0.008 | 0.0122 | 0.0276 | 0.0301 | 0.0047 | 0.2633 | 0.0052 | 0.0125 | 0.0009 | 0.0293 | 0.0037 |
Peroxisomes | 0.0003 | 0 | 0.0066 | 0 | 0.0041 | 0.0001 | 0.0005 | 0.0009 | 0.0003 | 0.0001 | 0.0028 | 0 | 0.0001 | 0 | 0.0149 | 0 | 0.0029 | 0 |
Punctate Nuclear | 0.0235 | 0.0582 | 0.093 | 0.0377 | 0.0028 | 0.0404 | 0.019 | 0.0813 | 0.0805 | 0.0174 | 0.0554 | 0.0257 | 0.0046 | 0.0003 | 0.4983 | 0 | 0.3476 | 0.0016 |
Vacuole | 0.0048 | 0.0107 | 0.0051 | 0.0061 | 0.0038 | 0.0046 | 0.0038 | 0.0076 | 0.0053 | 0.0032 | 0.0023 | 0.0014 | 0.002 | 0.009 | 0.0003 | 0.0045 | 0.0004 | 0.0004 |
Vacuole Periphery | 0.0235 | 0.0139 | 0.0187 | 0.0156 | 0.0105 | 0.0118 | 0.0315 | 0.0225 | 0.0294 | 0.0266 | 0.0112 | 0.0199 | 0.0081 | 0.1078 | 0.0006 | 0.0021 | 0.0002 | 0.027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.525 | 64.6412 | 53.2961 | 57.5458 | 59.0301 | 64.2404 | 73.6293 | 60.1784 | 50.4592 | 74.135 |
Translational Efficiency | 1.161 | 1.148 | 0.9316 | 1.0434 | 1.0967 | 1.248 | 0.9274 | 1.0164 | 1.1284 | 0.8944 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
15 | 1201 | 247 | 120 | 928 | 62 | 224 | 164 | 943 | 1263 | 471 | 284 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1558.43 | 1641.91 | 1763.68 | 1680.44 | 1317.50 | 1569.66 | 1879.59 | 1704.16 | 1321.33 | 1638.36 | 1818.80 | 1694.14 |
Standard Deviation | 293.23 | 331.83 | 272.34 | 272.36 | 223.46 | 225.60 | 219.89 | 278.54 | 226.75 | 327.79 | 255.43 | 276.20 |
Intensity Change Log 2 | — | 0.075282 | 0.178495 | 0.108746 | — | 0.252649 | 0.512615 | 0.371258 | — | 0.159250 | 0.341205 | 0.234957 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004026 | 0.000434 | 0.000126 | 0.004025 | 0.001147 | 0.000060 | 0.000043 | 0.000118 | 0.001193 | 0.000415 | 0.000087 | 0.001769 |
Bud Neck | 0.002351 | 0.004071 | 0.002763 | 0.006767 | 0.003701 | 0.001815 | 0.001652 | 0.005874 | 0.003679 | 0.003960 | 0.002234 | 0.006251 |
Bud Site | 0.005527 | 0.000777 | 0.000324 | 0.009565 | 0.002942 | 0.000888 | 0.001557 | 0.002961 | 0.002983 | 0.000782 | 0.000910 | 0.005751 |
Cell Periphery | 0.000920 | 0.000503 | 0.000729 | 0.003582 | 0.000779 | 0.000225 | 0.000413 | 0.003770 | 0.000782 | 0.000490 | 0.000579 | 0.003691 |
Cytoplasm | 0.029985 | 0.001323 | 0.001138 | 0.001254 | 0.004223 | 0.000809 | 0.000931 | 0.003433 | 0.004633 | 0.001298 | 0.001039 | 0.002513 |
Cytoplasmic Foci | 0.048250 | 0.004148 | 0.000097 | 0.006016 | 0.003464 | 0.002312 | 0.000419 | 0.004813 | 0.004176 | 0.004058 | 0.000250 | 0.005321 |
Eisosomes | 0.000051 | 0.000032 | 0.000008 | 0.000162 | 0.000026 | 0.000003 | 0.000003 | 0.000218 | 0.000026 | 0.000030 | 0.000005 | 0.000194 |
Endoplasmic Reticulum | 0.562273* | 0.325818* | 0.709952* | 0.753966* | 0.254730* | 0.665032* | 0.837087* | 0.710569* | 0.259622* | 0.342469* | 0.770415* | 0.728905* |
Endosome | 0.066514 | 0.023876 | 0.023084 | 0.037119 | 0.018428 | 0.027203 | 0.014568 | 0.047089 | 0.019193 | 0.024039 | 0.019034 | 0.042876 |
Golgi | 0.022013 | 0.001179 | 0.000947 | 0.006858 | 0.000578 | 0.000431 | 0.000630 | 0.006840 | 0.000919 | 0.001143 | 0.000797 | 0.006848 |
Lipid Particles | 0.015320 | 0.019425 | 0.001896 | 0.053520 | 0.011311 | 0.006397 | 0.001809 | 0.033406 | 0.011375 | 0.018785 | 0.001855 | 0.041905 |
Mitochondria | 0.002237 | 0.002695 | 0.000508 | 0.000732 | 0.003930 | 0.000172 | 0.000183 | 0.000370 | 0.003903 | 0.002571 | 0.000354 | 0.000523 |
Mitotic Spindle | 0.069653 | 0.002648 | 0.000044 | 0.003635 | 0.004742 | 0.000408 | 0.001066 | 0.009227 | 0.005774 | 0.002538 | 0.000531 | 0.006864 |
None | 0.000118 | 0.002200 | 0.000162 | 0.000083 | 0.001476 | 0.000164 | 0.000261 | 0.000421 | 0.001454 | 0.002100 | 0.000209 | 0.000278 |
Nuclear Periphery | 0.016765 | 0.334626* | 0.139210* | 0.026481 | 0.411895* | 0.149966* | 0.081300 | 0.036271 | 0.405610* | 0.325561* | 0.111669* | 0.032134 |
Nuclear Periphery Foci | 0.112866 | 0.252340* | 0.102782 | 0.062702 | 0.245252* | 0.141636 | 0.053062 | 0.117002 | 0.243146* | 0.246905* | 0.079136 | 0.094058 |
Nucleolus | 0.000007 | 0.006682 | 0.000038 | 0.000020 | 0.010742 | 0.000009 | 0.000017 | 0.000025 | 0.010571 | 0.006354 | 0.000028 | 0.000023 |
Nucleus | 0.007762 | 0.004571 | 0.000447 | 0.000087 | 0.010352 | 0.000107 | 0.000103 | 0.000445 | 0.010311 | 0.004352 | 0.000283 | 0.000294 |
Peroxisomes | 0.001167 | 0.000494 | 0.000005 | 0.000304 | 0.001220 | 0.000013 | 0.000023 | 0.000012 | 0.001219 | 0.000470 | 0.000014 | 0.000135 |
Vacuole | 0.030057 | 0.008175 | 0.014013 | 0.019517 | 0.006607 | 0.001882 | 0.004266 | 0.015220 | 0.006980 | 0.007866 | 0.009378 | 0.017036 |
Vacuole Periphery | 0.002139 | 0.003985 | 0.001728 | 0.003604 | 0.002454 | 0.000466 | 0.000605 | 0.001917 | 0.002449 | 0.003813 | 0.001194 | 0.002630 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.20 | 1.31 | -0.08 | -1.66 | -1.80 | 4.05 | 4.14 | 3.83 | -1.15 | -1.91 | 2.84 | 4.14 | -0.63 | -1.48 | -1.82 |
Bud Neck | -1.94 | -0.50 | -1.86 | -1.20 | -1.65 | 1.29 | 2.03 | -1.26 | -2.01 | -2.46 | -0.40 | 1.57 | -1.78 | -1.57 | -2.73 |
Bud Site | 1.32 | 1.48 | -1.01 | -2.42 | -2.55 | 2.13 | 0.68 | 0.08 | -1.62 | -0.58 | 3.38 | 1.99 | -1.58 | -2.96 | -2.60 |
Cell Periphery | 1.74 | 0.63 | -1.63 | -1.89 | -1.74 | 3.89 | 2.59 | -2.43 | -2.89 | -2.74 | 2.42 | 1.46 | -2.93 | -3.26 | -3.16 |
Cytoplasm | 1.01 | 1.02 | 1.02 | 0.07 | -0.25 | 3.94 | 3.70 | 0.15 | -1.54 | -1.46 | 3.51 | 3.71 | 1.23 | -1.28 | -1.46 |
Cytoplasmic Foci | 1.40 | 1.54 | 1.33 | -0.46 | -1.51 | 0.81 | 3.47 | -0.24 | -0.66 | -1.31 | 0.35 | 4.25 | -0.27 | -0.44 | -2.01 |
Eisosomes | 0.70 | 1.73 | -1.87 | -2.36 | -2.83 | 5.16 | 5.23 | -1.72 | -1.95 | -1.95 | -0.33 | 4.88 | -2.44 | -2.40 | -2.79 |
Endoplasmic Reticulum | 2.23 | -1.43 | -1.74 | -12.56 | -1.01 | -9.25 | -27.94 | -15.04 | -0.80 | 3.83 | -5.82 | -29.99 | -19.75 | -16.26 | 1.81 |
Endosome | 1.53 | 1.54 | 1.02 | -2.36 | -2.36 | -1.72 | 1.55 | -5.30 | -2.77 | -5.80 | -2.54 | -0.00 | -5.80 | -4.73 | -5.79 |
Golgi | 1.37 | 1.38 | 0.96 | -2.27 | -2.36 | 1.07 | -0.24 | -1.84 | -1.89 | -1.81 | -0.72 | 0.33 | -2.67 | -2.58 | -2.73 |
Lipid Particles | -0.41 | 1.61 | -2.35 | -2.48 | -3.76 | 1.46 | 5.39 | -2.61 | -3.06 | -3.77 | -2.91 | 5.79 | -3.97 | -3.00 | -5.29 |
Mitochondria | -0.27 | 1.05 | 0.88 | 1.99 | -0.35 | 6.46 | 6.45 | 5.94 | -1.40 | -1.33 | 1.40 | 6.16 | 5.43 | 2.50 | -0.65 |
Mitotic Spindle | 1.06 | 1.11 | 1.03 | -0.67 | -1.83 | 2.90 | 2.38 | -0.89 | -1.72 | -1.60 | 1.61 | 3.03 | -0.50 | -1.44 | -2.13 |
None | -2.89 | -0.28 | 0.27 | 2.97 | 0.68 | 4.02 | 3.45 | 2.93 | -1.45 | -0.73 | -0.84 | 3.80 | 3.56 | 2.67 | -0.54 |
Nuclear Periphery | -21.57* | -7.06 | -0.68 | 26.43* | 7.61 | 9.00 | 22.20* | 28.08* | 4.01 | 3.37 | 5.95 | 21.97 | 31.39 | 28.60* | 7.79 |
Nuclear Periphery Foci | -2.97 | 0.27 | 1.13 | 12.84 | 2.52 | 3.65 | 16.60 | 6.80 | 0.75 | -3.32 | -0.36 | 16.09 | 10.88 | 11.28 | -1.11 |
Nucleolus | -5.84 | -3.09 | -1.46 | 5.82 | 1.24 | 4.89 | 4.88 | 4.88 | -2.01 | -0.69 | 1.67 | 4.88 | 4.88 | 5.82 | 0.49 |
Nucleus | 0.39 | 0.95 | 1.00 | 5.28 | 1.54 | 6.58 | 6.59 | 6.27 | -1.67 | -1.74 | 3.37 | 6.49 | 6.48 | 4.94 | -0.09 |
Peroxisomes | 0.72 | 1.41 | 0.92 | 0.58 | -1.31 | 2.70 | 2.66 | 2.71 | 0.25 | 0.63 | 1.55 | 2.73 | 2.36 | 1.78 | -1.24 |
Vacuole | 2.21 | 1.61 | 0.88 | -1.78 | -0.86 | 4.41 | 1.45 | -2.16 | -3.31 | -2.61 | -0.62 | -1.94 | -2.79 | -2.57 | -2.09 |
Vacuole Periphery | -1.24 | 0.24 | -0.56 | 0.11 | -0.79 | 7.41 | 6.01 | 0.85 | -2.55 | -2.20 | -2.46 | 4.26 | -0.20 | 0.95 | -1.33 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|