Standard name | |
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Description | Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 9 | 8 | 1 | 2 | – | 7 | 1 | 3 | 4 | 4 | 2 | 7 | 14 | 0 | 2 | 0 | 0 | 2 | 7 | 2 | 4 | 8 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | – | 1 | 1 | 4 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 8 | 0 | 0 | 0 | – | 3 | 1 | 0 | 2 | 3 | 4 | 14 | 29 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 4 | 15 | 10 | 17 | 10 | – | 54 | 11 | 8 | 15 | 20 | 7 | 23 | 35 | 5 | 16 | 6 | 0 | 0 | 2 | 0 | 0 | 4 |
Endoplasmic Reticulum | 45 | 65 | 79 | 15 | 3 | 7 | – | 32 | 17 | 18 | 20 | 25 | 123 | 374 | 79 | 0 | 3 | 7 | 76 | 81 | 457 | 306 | 410 | 530 |
Endosome | 0 | 0 | 3 | 4 | 10 | 6 | – | 3 | 4 | 9 | 8 | 8 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 4 | 15 |
Golgi | 3 | 0 | 36 | 14 | 6 | 1 | – | 23 | 6 | 7 | 1 | 12 | 12 | 30 | 19 | 0 | 12 | 16 | 0 | 0 | 1 | 3 | 5 | 6 |
Mitochondria | 0 | 1 | 9 | 12 | 7 | 7 | – | 13 | 10 | 23 | 19 | 18 | 0 | 0 | 0 | 2 | 13 | 12 | 0 | 0 | 3 | 4 | 5 | 11 |
Nucleus | 1 | 0 | 2 | 3 | 3 | 20 | – | 25 | 9 | 23 | 29 | 29 | 3 | 2 | 4 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 34 | 19 | 87 | 113 | 107 | 192 | – | 338 | 261 | 249 | 187 | 282 | 142 | 114 | 49 | 4 | 27 | 25 | 7 | 20 | 51 | 155 | 123 | 154 |
Nucleolus | 1 | 0 | 3 | 9 | 41 | 21 | – | 64 | 50 | 31 | 32 | 61 | 1 | 0 | 6 | 0 | 3 | 4 | 0 | 0 | 0 | 2 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 13 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 7 |
Vac/Vac Membrane | 10 | 19 | 163 | 149 | 228 | 205 | – | 355 | 203 | 316 | 178 | 349 | 72 | 34 | 149 | 6 | 63 | 34 | 0 | 3 | 6 | 25 | 34 | 86 |
Unique Cell Count | 64 | 90 | 349 | 303 | 400 | 420 | 787 | 468 | 598 | 421 | 681 | 283 | 454 | 336 | 16 | 119 | 96 | 87 | 111 | 543 | 511 | 598 | 845 | |
Labelled Cell Count | 94 | 113 | 416 | 338 | 440 | 474 | 918 | 575 | 691 | 500 | 814 | 366 | 598 | 391 | 19 | 143 | 108 | 87 | 111 | 543 | 511 | 598 | 845 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.8 | 14.1 | 21.1 | 21.2 | 20.2 | 16.5 | – | 17.2 | 15.9 | 16.0 | 15.8 | 16.4 | 17.5 | 18.6 | 18.5 | 12.8 | 18.8 | 22.7 | 20.6 | 21.0 | 21.8 |
Std Deviation (1e-4) | 2.9 | 2.7 | 4.3 | 4.4 | 4.5 | 3.5 | – | 5.2 | 3.9 | 3.7 | 4.4 | 4.1 | 2.9 | 3.2 | 3.6 | 3.2 | 4.7 | 5.6 | 3.2 | 3.7 | 4.0 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.06 | -0.35 | – | -0.29 | -0.41 | -0.4 | -0.41 | -0.36 | -0.26 | -0.18 | -0.19 | -0.72 | -0.16 | 0.11 | -0.03 | -0.01 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.7 | 0 | 0 | -1.0 | 1.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -0.8 | 0.7 | 3.7 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 0 | -1.5 | – | 1.7 | -1.6 | -2.9 | -0.5 | -1.1 | -1.2 | 0.5 | 3.1 | 0 | 3.5 | 0 |
Endoplasmic Reticulum | -6.4 | -9.6 | -9.2 | – | -9.7 | -8.3 | -9.6 | -7.4 | -9.6 | 5.6 | 16.9 | 0.3 | 0 | -5.0 | -3.4 |
Endosome | 0 | 1.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.7 | -5.2 | -6.5 | – | -5.2 | -5.8 | -6.5 | -6.5 | -6.2 | -2.9 | -1.9 | -2.2 | 0 | -0.1 | 1.7 |
Mitochondria | 1.0 | -0.8 | -0.9 | – | -1.0 | -0.4 | 1.0 | 1.4 | 0.1 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Nucleus | 0 | 0 | 3.5 | – | 2.7 | 0 | 3.0 | 4.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3.4 | 0.6 | 6.0 | – | 5.8 | 8.8 | 5.2 | 5.6 | 5.2 | 6.6 | 0.1 | -3.4 | 0 | -0.5 | 0.2 |
Nucleolus | 2.0 | 5.5 | 3.3 | – | 4.8 | 5.6 | 3.5 | 4.5 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 2.8 | 0.6 | – | -0.5 | -0.9 | 1.8 | -1.2 | 1.4 | -5.5 | -12.8 | -0.6 | -0.7 | 1.2 | -2.0 |