Standard name
Human Ortholog
Description Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0.05 0.1 0.31 0.13 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.7 0.72 0.23 0.05 0 0 – 0 0 0 0.05 0 0.43 0.82 0.24 0 0 0.07 0.88 0.73 0.84 0.6 0.69 0.63
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0.1 0.05 0 0 – 0 0 0 0 0 0 0.07 0.06 0 0.1 0.17 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0.12 0.11 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 – 0 0 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.53 0.21 0.25 0.37 0.27 0.46 – 0.43 0.56 0.42 0.44 0.41 0.5 0.25 0.15 0.25 0.23 0.26 0.09 0.18 0.1 0.3 0.21 0.18
Nucleolus 0 0 0 0 0.1 0.05 – 0.08 0.11 0.05 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.21 0.47 0.49 0.57 0.49 – 0.45 0.43 0.53 0.42 0.51 0.25 0.07 0.44 0.38 0.53 0.35 0 0 0 0.05 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 9 8 1 2 – 7 1 3 4 4 2 7 14 0 2 0 0 2 7 2 4 8
Bud 0 1 0 1 0 2 – 1 1 4 4 3 0 0 1 0 0 0 0 0 3 2 5 12
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 8 0 0 0 – 3 1 0 2 3 4 14 29 1 0 3 0 0 0 0 0 0
Cytoplasm 0 4 15 10 17 10 – 54 11 8 15 20 7 23 35 5 16 6 0 0 2 0 0 4
Endoplasmic Reticulum 45 65 79 15 3 7 – 32 17 18 20 25 123 374 79 0 3 7 76 81 457 306 410 530
Endosome 0 0 3 4 10 6 – 3 4 9 8 8 0 0 1 0 1 0 0 0 1 4 4 15
Golgi 3 0 36 14 6 1 – 23 6 7 1 12 12 30 19 0 12 16 0 0 1 3 5 6
Mitochondria 0 1 9 12 7 7 – 13 10 23 19 18 0 0 0 2 13 12 0 0 3 4 5 11
Nucleus 1 0 2 3 3 20 – 25 9 23 29 29 3 2 4 1 3 1 0 0 0 0 1 1
Nuclear Periphery 34 19 87 113 107 192 – 338 261 249 187 282 142 114 49 4 27 25 7 20 51 155 123 154
Nucleolus 1 0 3 9 41 21 – 64 50 31 32 61 1 0 6 0 3 4 0 0 0 2 1 1
Peroxisomes 0 0 0 0 4 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 2 0 13 0 – 0 0 0 0 0 0 0 5 0 0 0 0 0 2 1 0 7
Vac/Vac Membrane 10 19 163 149 228 205 – 355 203 316 178 349 72 34 149 6 63 34 0 3 6 25 34 86
Unique Cell Count 64 90 349 303 400 420 787 468 598 421 681 283 454 336 16 119 96 87 111 543 511 598 845
Labelled Cell Count 94 113 416 338 440 474 918 575 691 500 814 366 598 391 19 143 108 87 111 543 511 598 845


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.8 14.1 21.1 21.2 20.2 16.5 – 17.2 15.9 16.0 15.8 16.4 17.5 18.6 18.5 12.8 18.8 22.7 20.6 21.0 21.8
Std Deviation (1e-4) 2.9 2.7 4.3 4.4 4.5 3.5 – 5.2 3.9 3.7 4.4 4.1 2.9 3.2 3.6 3.2 4.7 5.6 3.2 3.7 4.0
Intensity Change (Log2) – – – 0.01 -0.06 -0.35 – -0.29 -0.41 -0.4 -0.41 -0.36 -0.26 -0.18 -0.19 -0.72 -0.16 0.11 -0.03 -0.01 0.05

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP70001020WT3HU80HU120HU16001020WT3rpd3Δ_1rpd3Δ_2rpd3Δ_301020WT1AF100AF140AF18001020
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 -1.7 0 0 -1.0 1.2 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 -0.8 0.7 3.7 0 0 0
Cytoplasm -0.7 0 -1.5 – 1.7 -1.6 -2.9 -0.5 -1.1 -1.2 0.5 3.1 0 3.5 0
Endoplasmic Reticulum -6.4 -9.6 -9.2 – -9.7 -8.3 -9.6 -7.4 -9.6 5.6 16.9 0.3 0 -5.0 -3.4
Endosome 0 1.7 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.7 -5.2 -6.5 – -5.2 -5.8 -6.5 -6.5 -6.2 -2.9 -1.9 -2.2 0 -0.1 1.7
Mitochondria 1.0 -0.8 -0.9 – -1.0 -0.4 1.0 1.4 0.1 0 0 0 0 3.7 0
Nucleus 0 0 3.5 – 2.7 0 3.0 4.4 3.3 0 0 0 0 0 0
Nuclear Periphery 3.4 0.6 6.0 – 5.8 8.8 5.2 5.6 5.2 6.6 0.1 -3.4 0 -0.5 0.2
Nucleolus 2.0 5.5 3.3 – 4.8 5.6 3.5 4.5 5.1 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.6 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 2.8 0.6 – -0.5 -0.9 1.8 -1.2 1.4 -5.5 -12.8 -0.6 -0.7 1.2 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0095 0.0002 0.0041 0.0005 0.0119 0.0006 0.0279 0.0004 0.0154 0.0006 0.0188 0.0004 0.0145 0.0017 0.0027 0.0002 0.0004 0.0008
Bud 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0002 0.0001 0.0004 0 0.0002 0
Bud Neck 0.0007 0.0004 0.0005 0.0002 0.0005 0.0004 0.0002 0.0004 0.0002 0.0001 0.0006 0.0001 0.0002 0.0002 0.0009 0.0001 0.0012 0.0001
Bud Periphery 0.0002 0.0001 0.0003 0.0001 0.0007 0.0001 0.0002 0.0003 0.0002 0.0001 0.0003 0 0.0002 0.0007 0.0003 0.0001 0.0002 0.0001
Bud Site 0.0004 0.0001 0.0008 0 0.0006 0.0002 0.0006 0.0003 0.0013 0 0.001 0 0.0003 0.0001 0.0064 0 0.0041 0
Cell Periphery 0.0004 0.0026 0.0012 0.0007 0.0002 0.0004 0.0004 0.002 0.0011 0.0003 0.0002 0.0001 0.0001 0.0019 0.0003 0.0008 0.0003 0.0002
Cytoplasm 0.004 0.0039 0.0058 0.0014 0.0005 0.001 0.0203 0.0004 0.001 0.0008 0.0007 0.0002 0.0167 0.0003 0.0003 0.0005 0.0002 0
Cytoplasmic Foci 0.0049 0.0224 0.0173 0.0059 0.0041 0.006 0.0122 0.0778 0.0265 0.015 0.0116 0.0036 0.0003 0.0001 0.1909 0 0.0781 0.0001
Eisosomes 0.0005 0.0001 0.0002 0 0.0001 0 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0.0001 0.0008 0 0.0002 0.0002
Endoplasmic Reticulum 0.1102 0.3321 0.2091 0.4244 0.3367 0.2091 0.1057 0.333 0.2831 0.4548 0.425 0.1821 0.0172 0.1325 0.0001 0.944 0.0001 0.0073
Endosome 0.0034 0.0006 0.0076 0.0011 0.0111 0.0026 0.0262 0.0009 0.002 0.001 0.0093 0.0005 0.0103 0.0024 0.0008 0.0016 0.0009 0.0004
Golgi 0.0025 0.0003 0.0028 0.0003 0.009 0.0009 0.0065 0.0015 0.0017 0.0006 0.0074 0.0008 0.0003 0.0014 0.0037 0.0002 0.0023 0.001
Lipid Particles 0.006 0.0098 0.0147 0.0082 0.0147 0.0143 0.032 0.0644 0.0595 0.0477 0.067 0.0155 0.0001 0.0025 0.099 0.0003 0.0658 0.0045
Mitochondria 0.0013 0.0005 0.0023 0.0004 0.0039 0.0014 0.0019 0.0006 0.0004 0.0007 0.0029 0.0001 0.0098 0.003 0.0111 0.0001 0.0011 0.0008
None 0.0052 0.0001 0.0014 0.0001 0.0004 0.0008 0.0093 0.0003 0.0002 0.0001 0.0005 0.0001 0.004 0.0003 0.0014 0 0.0005 0.0001
Nuclear Periphery 0.7694 0.4806 0.517 0.4826 0.5348 0.6825 0.6859 0.3548 0.4364 0.4003 0.3225 0.7421 0.6467 0.7304 0.0011 0.0445 0.0009 0.9502
Nucleolus 0.0092 0.0213 0.0534 0.0036 0.0025 0.0062 0.0015 0.0422 0.0432 0.0028 0.0301 0.0027 0.0008 0.0001 0.153 0 0.463 0.0015
Nucleus 0.0199 0.0421 0.0381 0.0109 0.0468 0.0162 0.0142 0.008 0.0122 0.0276 0.0301 0.0047 0.2633 0.0052 0.0125 0.0009 0.0293 0.0037
Peroxisomes 0.0003 0 0.0066 0 0.0041 0.0001 0.0005 0.0009 0.0003 0.0001 0.0028 0 0.0001 0 0.0149 0 0.0029 0
Punctate Nuclear 0.0235 0.0582 0.093 0.0377 0.0028 0.0404 0.019 0.0813 0.0805 0.0174 0.0554 0.0257 0.0046 0.0003 0.4983 0 0.3476 0.0016
Vacuole 0.0048 0.0107 0.0051 0.0061 0.0038 0.0046 0.0038 0.0076 0.0053 0.0032 0.0023 0.0014 0.002 0.009 0.0003 0.0045 0.0004 0.0004
Vacuole Periphery 0.0235 0.0139 0.0187 0.0156 0.0105 0.0118 0.0315 0.0225 0.0294 0.0266 0.0112 0.0199 0.0081 0.1078 0.0006 0.0021 0.0002 0.027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.525 64.6412 53.2961 57.5458 59.0301 64.2404 73.6293 60.1784 50.4592 74.135
Translational Efficiency 1.161 1.148 0.9316 1.0434 1.0967 1.248 0.9274 1.0164 1.1284 0.8944

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
15 1201 247 120 928 62 224 164 943 1263 471 284

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1558.43 1641.91 1763.68 1680.44 1317.50 1569.66 1879.59 1704.16 1321.33 1638.36 1818.80 1694.14
Standard Deviation 293.23 331.83 272.34 272.36 223.46 225.60 219.89 278.54 226.75 327.79 255.43 276.20
Intensity Change Log 2 — 0.075282 0.178495 0.108746 — 0.252649 0.512615 0.371258 — 0.159250 0.341205 0.234957

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004026 0.000434 0.000126 0.004025 0.001147 0.000060 0.000043 0.000118 0.001193 0.000415 0.000087 0.001769
Bud Neck 0.002351 0.004071 0.002763 0.006767 0.003701 0.001815 0.001652 0.005874 0.003679 0.003960 0.002234 0.006251
Bud Site 0.005527 0.000777 0.000324 0.009565 0.002942 0.000888 0.001557 0.002961 0.002983 0.000782 0.000910 0.005751
Cell Periphery 0.000920 0.000503 0.000729 0.003582 0.000779 0.000225 0.000413 0.003770 0.000782 0.000490 0.000579 0.003691
Cytoplasm 0.029985 0.001323 0.001138 0.001254 0.004223 0.000809 0.000931 0.003433 0.004633 0.001298 0.001039 0.002513
Cytoplasmic Foci 0.048250 0.004148 0.000097 0.006016 0.003464 0.002312 0.000419 0.004813 0.004176 0.004058 0.000250 0.005321
Eisosomes 0.000051 0.000032 0.000008 0.000162 0.000026 0.000003 0.000003 0.000218 0.000026 0.000030 0.000005 0.000194
Endoplasmic Reticulum 0.562273 0.325818 0.709952 0.753966 0.254730 0.665032 0.837087 0.710569 0.259622 0.342469 0.770415 0.728905
Endosome 0.066514 0.023876 0.023084 0.037119 0.018428 0.027203 0.014568 0.047089 0.019193 0.024039 0.019034 0.042876
Golgi 0.022013 0.001179 0.000947 0.006858 0.000578 0.000431 0.000630 0.006840 0.000919 0.001143 0.000797 0.006848
Lipid Particles 0.015320 0.019425 0.001896 0.053520 0.011311 0.006397 0.001809 0.033406 0.011375 0.018785 0.001855 0.041905
Mitochondria 0.002237 0.002695 0.000508 0.000732 0.003930 0.000172 0.000183 0.000370 0.003903 0.002571 0.000354 0.000523
Mitotic Spindle 0.069653 0.002648 0.000044 0.003635 0.004742 0.000408 0.001066 0.009227 0.005774 0.002538 0.000531 0.006864
None 0.000118 0.002200 0.000162 0.000083 0.001476 0.000164 0.000261 0.000421 0.001454 0.002100 0.000209 0.000278
Nuclear Periphery 0.016765 0.334626 0.139210 0.026481 0.411895 0.149966 0.081300 0.036271 0.405610 0.325561 0.111669 0.032134
Nuclear Periphery Foci 0.112866 0.252340 0.102782 0.062702 0.245252 0.141636 0.053062 0.117002 0.243146 0.246905 0.079136 0.094058
Nucleolus 0.000007 0.006682 0.000038 0.000020 0.010742 0.000009 0.000017 0.000025 0.010571 0.006354 0.000028 0.000023
Nucleus 0.007762 0.004571 0.000447 0.000087 0.010352 0.000107 0.000103 0.000445 0.010311 0.004352 0.000283 0.000294
Peroxisomes 0.001167 0.000494 0.000005 0.000304 0.001220 0.000013 0.000023 0.000012 0.001219 0.000470 0.000014 0.000135
Vacuole 0.030057 0.008175 0.014013 0.019517 0.006607 0.001882 0.004266 0.015220 0.006980 0.007866 0.009378 0.017036
Vacuole Periphery 0.002139 0.003985 0.001728 0.003604 0.002454 0.000466 0.000605 0.001917 0.002449 0.003813 0.001194 0.002630

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.20 1.31 -0.08 -1.66 -1.80 4.05 4.14 3.83 -1.15 -1.91 2.84 4.14 -0.63 -1.48 -1.82
Bud Neck -1.94 -0.50 -1.86 -1.20 -1.65 1.29 2.03 -1.26 -2.01 -2.46 -0.40 1.57 -1.78 -1.57 -2.73
Bud Site 1.32 1.48 -1.01 -2.42 -2.55 2.13 0.68 0.08 -1.62 -0.58 3.38 1.99 -1.58 -2.96 -2.60
Cell Periphery 1.74 0.63 -1.63 -1.89 -1.74 3.89 2.59 -2.43 -2.89 -2.74 2.42 1.46 -2.93 -3.26 -3.16
Cytoplasm 1.01 1.02 1.02 0.07 -0.25 3.94 3.70 0.15 -1.54 -1.46 3.51 3.71 1.23 -1.28 -1.46
Cytoplasmic Foci 1.40 1.54 1.33 -0.46 -1.51 0.81 3.47 -0.24 -0.66 -1.31 0.35 4.25 -0.27 -0.44 -2.01
Eisosomes 0.70 1.73 -1.87 -2.36 -2.83 5.16 5.23 -1.72 -1.95 -1.95 -0.33 4.88 -2.44 -2.40 -2.79
Endoplasmic Reticulum 2.23 -1.43 -1.74 -12.56 -1.01 -9.25 -27.94 -15.04 -0.80 3.83 -5.82 -29.99 -19.75 -16.26 1.81
Endosome 1.53 1.54 1.02 -2.36 -2.36 -1.72 1.55 -5.30 -2.77 -5.80 -2.54 -0.00 -5.80 -4.73 -5.79
Golgi 1.37 1.38 0.96 -2.27 -2.36 1.07 -0.24 -1.84 -1.89 -1.81 -0.72 0.33 -2.67 -2.58 -2.73
Lipid Particles -0.41 1.61 -2.35 -2.48 -3.76 1.46 5.39 -2.61 -3.06 -3.77 -2.91 5.79 -3.97 -3.00 -5.29
Mitochondria -0.27 1.05 0.88 1.99 -0.35 6.46 6.45 5.94 -1.40 -1.33 1.40 6.16 5.43 2.50 -0.65
Mitotic Spindle 1.06 1.11 1.03 -0.67 -1.83 2.90 2.38 -0.89 -1.72 -1.60 1.61 3.03 -0.50 -1.44 -2.13
None -2.89 -0.28 0.27 2.97 0.68 4.02 3.45 2.93 -1.45 -0.73 -0.84 3.80 3.56 2.67 -0.54
Nuclear Periphery -21.57 -7.06 -0.68 26.43 7.61 9.00 22.20 28.08 4.01 3.37 5.95 21.97 31.39 28.60 7.79
Nuclear Periphery Foci -2.97 0.27 1.13 12.84 2.52 3.65 16.60 6.80 0.75 -3.32 -0.36 16.09 10.88 11.28 -1.11
Nucleolus -5.84 -3.09 -1.46 5.82 1.24 4.89 4.88 4.88 -2.01 -0.69 1.67 4.88 4.88 5.82 0.49
Nucleus 0.39 0.95 1.00 5.28 1.54 6.58 6.59 6.27 -1.67 -1.74 3.37 6.49 6.48 4.94 -0.09
Peroxisomes 0.72 1.41 0.92 0.58 -1.31 2.70 2.66 2.71 0.25 0.63 1.55 2.73 2.36 1.78 -1.24
Vacuole 2.21 1.61 0.88 -1.78 -0.86 4.41 1.45 -2.16 -3.31 -2.61 -0.62 -1.94 -2.79 -2.57 -2.09
Vacuole Periphery -1.24 0.24 -0.56 0.11 -0.79 7.41 6.01 0.85 -2.55 -2.20 -2.46 4.26 -0.20 0.95 -1.33
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
Localization
Cell Percentages ER (64%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Lag1

Lag1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lag1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available