Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 3 | 1 | 8 | 14 | 9 |
Bud | 8 | 6 | 16 | 9 | 10 | 7 | 19 | 10 | 16 | 22 | 15 | 7 | 13 | 7 | 8 | 9 | 8 | 6 | 1 | 1 | 4 | 2 | 2 | 6 |
Bud Neck | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 1 | 0 | 0 | 1 | 5 | 4 |
Bud Site | 9 | 4 | 2 | 2 | 0 | 1 | 4 | 5 | 3 | 6 | 4 | 3 | 2 | 1 | 1 | 5 | 1 | 6 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 6 | 3 | 3 | 4 | 1 | 2 | 2 | 3 | 3 | 0 | 3 | 4 | 3 | 16 | 13 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 7 | 7 | 6 | 10 | 5 | 11 | 12 | 7 | 10 | 4 | 5 | 9 | 7 | 18 | 21 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 4 | 9 | 7 | 9 | 0 | 0 | 0 | 1 | 2 | 4 |
Endosome | 24 | 22 | 29 | 18 | 27 | 16 | 10 | 15 | 2 | 2 | 0 | 3 | 12 | 63 | 60 | 73 | 49 | 39 | 31 | 26 | 36 | 23 | 34 | 27 |
Golgi | 0 | 3 | 1 | 0 | 8 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 24 | 12 | 24 | 12 | 11 | 7 | 7 | 18 | 18 |
Mitochondria | 223 | 197 | 175 | 142 | 162 | 224 | 318 | 271 | 263 | 292 | 199 | 181 | 259 | 151 | 199 | 19 | 25 | 28 | 196 | 178 | 190 | 142 | 138 | 148 |
Nucleus | 3 | 3 | 2 | 2 | 4 | 10 | 21 | 19 | 9 | 8 | 5 | 3 | 0 | 1 | 3 | 8 | 3 | 9 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 4 | 2 | 0 | 0 | 5 | 6 | 6 | 12 | 9 | 9 | 7 | 5 | 3 | 0 | 1 | 2 | 2 | 0 | 1 | 2 | 0 | 1 | 3 | 2 |
Nucleolus | 17 | 6 | 3 | 0 | 1 | 10 | 11 | 10 | 10 | 7 | 7 | 5 | 13 | 3 | 13 | 14 | 12 | 11 | 1 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 36 | 30 | 34 | 23 | 18 | 7 | 11 | 6 | 2 | 1 | 3 | 1 | 49 | 91 | 76 | 117 | 59 | 62 | 19 | 11 | 26 | 24 | 46 | 33 |
SpindlePole | 25 | 18 | 56 | 18 | 25 | 15 | 30 | 24 | 10 | 6 | 7 | 10 | 25 | 39 | 38 | 134 | 104 | 82 | 17 | 10 | 15 | 5 | 6 | 13 |
Vac/Vac Membrane | 33 | 48 | 25 | 13 | 27 | 35 | 28 | 32 | 14 | 18 | 4 | 6 | 15 | 17 | 26 | 48 | 40 | 43 | 4 | 13 | 3 | 5 | 4 | 8 |
Unique Cell Count | 274 | 253 | 259 | 176 | 224 | 268 | 376 | 339 | 287 | 316 | 209 | 196 | 313 | 257 | 347 | 367 | 248 | 251 | 295 | 263 | 291 | 229 | 288 | 286 |
Labelled Cell Count | 390 | 354 | 358 | 236 | 307 | 343 | 471 | 421 | 348 | 388 | 261 | 230 | 405 | 386 | 455 | 504 | 350 | 332 | 295 | 263 | 291 | 229 | 288 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.0 | 6.0 | 5.4 | 6.3 | 5.1 | 5.3 | 5.5 | 4.9 | 4.4 | 4.0 | 4.5 | 5.8 | 6.2 | 6.2 | 8.2 | 8.7 | 8.4 | 5.8 | 5.8 | 6.1 |
Std Deviation (1e-4) | 1.0 | 1.1 | 0.9 | 0.8 | 1.3 | 1.1 | 1.2 | 1.2 | 1.5 | 1.0 | 0.9 | 0.8 | 1.2 | 1.6 | 1.5 | 1.7 | 2.0 | 1.9 | 1.5 | 1.9 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.08 | -0.23 | -0.17 | -0.11 | -0.29 | -0.43 | -0.56 | -0.41 | -0.03 | 0.06 | 0.05 | 0.46 | 0.54 | 0.5 | -0.05 | -0.03 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.5 | -0.8 | -2.0 | -0.6 | -1.9 | -0.3 | 0 | 0 | 0 | -1.1 | -1.9 | -2.4 | -2.3 | -1.6 | -2.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 1.7 | 0.1 |
Cytoplasm | 0.4 | 1.0 | -0.6 | 0.2 | 0.6 | -0.2 | 0.3 | 0 | -0.1 | 0.1 | 0 | 1.5 | 1.8 | 1.1 | 0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.3 | 0.3 | -2.1 | -4.4 | -3.1 | -5.3 | 0 | 0 | 0 | -3.4 | 4.0 | 2.1 | 2.9 | 2.7 | 1.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 3.2 | 4.8 |
Mitochondria | 3.0 | 1.1 | 4.3 | 5.1 | 3.4 | 7.1 | 0 | 0 | 0 | 4.2 | -2.1 | -2.6 | -16.6 | -13.2 | -13.0 |
Nucleus | 0 | 0 | 2.3 | 3.2 | 3.2 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.9 | 1.5 | 1.5 | 1.8 | 0 | 0 | 0 | 2.2 | 0 | 2.0 | 2.0 | 2.4 | 2.2 |
Peroxisomes | 0 | -1.8 | -4.5 | -4.9 | -5.5 | -5.8 | 0 | 0 | 0 | 0.9 | 5.9 | 2.8 | 5.4 | 3.1 | 3.3 |
SpindlePole | -3.1 | -3.1 | -5.4 | -4.9 | -5.2 | -6.5 | 0 | 0 | 0 | -4.7 | -1.9 | -3.6 | 4.0 | 4.9 | 2.8 |
Vacuole | -0.8 | 0.8 | 1.2 | -1.0 | -0.1 | -2.2 | -1.8 | -3.5 | -2.8 | -2.3 | -1.3 | -0.9 | 1.3 | 2.2 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7436 | 3.4158 | 3.0457 | 2.8999 | 2.847 | 3.1996 | 4.4743 | 5.0091 | 4.7729 | 5.0321 | 4.4671 | 4.7343 | 4.3356 | 4.631 | 4.7353 | 4.2307 | 4.009 | 4.7844 |
Actin | 0.0256 | 0.0044 | 0.0117 | 0.0004 | 0.0016 | 0.0059 | 0.0315 | 0.002 | 0.0146 | 0.0003 | 0.0048 | 0.0016 | 0.0416 | 0.0113 | 0.0332 | 0.0066 | 0.0025 | 0.0016 |
Bud | 0.0006 | 0.0004 | 0.0015 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0 |
Bud Neck | 0.0008 | 0.0003 | 0.0005 | 0.0169 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0003 | 0.001 | 0.0005 | 0.0002 | 0.0002 | 0.0002 |
Bud Periphery | 0.0008 | 0.0004 | 0.0026 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0007 | 0 | 0.0005 | 0.0004 | 0.001 | 0.0002 | 0.0013 | 0.0001 |
Bud Site | 0.0036 | 0.0045 | 0.0019 | 0.0337 | 0.0001 | 0.0002 | 0.0025 | 0.0003 | 0.0004 | 0.0001 | 0.0027 | 0 | 0.0011 | 0.0024 | 0.0033 | 0.0003 | 0.0005 | 0.0001 |
Cell Periphery | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Cytoplasm | 0.0035 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0047 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 |
Cytoplasmic Foci | 0.0322 | 0.0109 | 0.0026 | 0.0075 | 0.0019 | 0.0063 | 0.0097 | 0.0041 | 0.0057 | 0.0144 | 0.0096 | 0.0015 | 0.0197 | 0.0045 | 0.0112 | 0.0287 | 0.0006 | 0.0008 |
Eisosomes | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0001 | 0.0057 | 0.0001 |
Endoplasmic Reticulum | 0.0028 | 0.0001 | 0.0002 | 0 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0049 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 |
Endosome | 0.0444 | 0.0147 | 0.0121 | 0.0017 | 0.0199 | 0.0081 | 0.0133 | 0.0115 | 0.0142 | 0.0208 | 0.0317 | 0.0039 | 0.0176 | 0.0094 | 0.0122 | 0.0099 | 0.0024 | 0.0014 |
Golgi | 0.0314 | 0.0396 | 0.01 | 0.003 | 0.0186 | 0.0198 | 0.0322 | 0.0232 | 0.0406 | 0.0151 | 0.0136 | 0.0215 | 0.0169 | 0.0221 | 0.0182 | 0.0202 | 0.0024 | 0.0038 |
Lipid Particles | 0.0504 | 0.019 | 0.0195 | 0.0654 | 0.0109 | 0.0068 | 0.02 | 0.005 | 0.0069 | 0.0437 | 0.0105 | 0.0046 | 0.0245 | 0.006 | 0.0175 | 0.0471 | 0.0032 | 0.0033 |
Mitochondria | 0.7315 | 0.8821 | 0.9229 | 0.8537 | 0.9201 | 0.9411 | 0.8531 | 0.9346 | 0.8933 | 0.873 | 0.9042 | 0.9377 | 0.8148 | 0.9289 | 0.8623 | 0.8446 | 0.9699 | 0.982 |
None | 0.0022 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0108 | 0.0001 | 0.0034 | 0.0002 | 0.0001 | 0 |
Nuclear Periphery | 0.0057 | 0 | 0.0004 | 0.0003 | 0.0169 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0078 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 |
Nucleolus | 0.0012 | 0.0009 | 0.0005 | 0.0031 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0 |
Nucleus | 0.0046 | 0.0002 | 0.0006 | 0.0004 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0066 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 |
Peroxisomes | 0.0457 | 0.0164 | 0.0064 | 0.0056 | 0.0015 | 0.0086 | 0.0213 | 0.0167 | 0.0146 | 0.0261 | 0.0077 | 0.0281 | 0.0098 | 0.009 | 0.0186 | 0.0365 | 0.0029 | 0.0053 |
Punctate Nuclear | 0.0063 | 0.0001 | 0.0003 | 0.0011 | 0.0024 | 0.0002 | 0.0032 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0115 | 0 | 0.0017 | 0.0019 | 0 | 0 |
Vacuole | 0.0028 | 0.0029 | 0.0023 | 0.0011 | 0.0008 | 0.0003 | 0.0027 | 0.0006 | 0.0036 | 0.0019 | 0.0058 | 0.0001 | 0.0014 | 0.001 | 0.0064 | 0.0009 | 0.004 | 0.0003 |
Vacuole Periphery | 0.0031 | 0.0025 | 0.0033 | 0.0053 | 0.0024 | 0.0019 | 0.0076 | 0.0011 | 0.0033 | 0.0031 | 0.0054 | 0.0006 | 0.0028 | 0.0026 | 0.0067 | 0.0018 | 0.0029 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.0888 | 8.0816 | 4.4187 | 7.4167 | 8.3283 | 9.0354 | 8.8164 | 10.3044 | 8.4849 | 10.8775 |
Translational Efficiency | 0.8373 | 0.8335 | 0.857 | 0.6183 | 0.6938 | 0.6958 | 0.7745 | 0.5861 | 0.687 | 0.6054 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2164 | 1204 | 290 | 918 | 1566 | 1774 | 2502 | 2592 | 3730 | 2978 | 2792 | 3510 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 678.62 | 755.93 | 1113.69 | 973.72 | 696.93 | 773.71 | 971.63 | 975.20 | 686.31 | 766.52 | 986.39 | 974.81 |
Standard Deviation | 75.85 | 123.59 | 138.95 | 136.98 | 98.11 | 112.10 | 123.78 | 131.40 | 86.38 | 117.21 | 132.72 | 132.88 |
Intensity Change Log 2 | — | 0.155649 | 0.714672 | 0.520903 | — | 0.150779 | 0.479393 | 0.484684 | — | 0.153184 | 0.600260 | 0.502666 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000451 | 0.002808 | 0.004275 | 0.027840 | 0.001066 | 0.002254 | 0.001395 | 0.008410 | 0.000709 | 0.002478 | 0.001694 | 0.013492 |
Bud Neck | 0.014463 | 0.006405 | 0.012155 | 0.004148 | 0.004958 | 0.002381 | 0.001864 | 0.001853 | 0.010472 | 0.004008 | 0.002933 | 0.002453 |
Bud Site | 0.022052 | 0.019602 | 0.028018 | 0.056531 | 0.007656 | 0.007751 | 0.006867 | 0.026332 | 0.016008 | 0.012542 | 0.009064 | 0.034230 |
Cell Periphery | 0.000567 | 0.000555 | 0.000564 | 0.000671 | 0.001107 | 0.000323 | 0.000163 | 0.000396 | 0.000794 | 0.000417 | 0.000205 | 0.000468 |
Cytoplasm | 0.012094 | 0.006592 | 0.001450 | 0.012093 | 0.002748 | 0.002999 | 0.001736 | 0.007166 | 0.008170 | 0.004452 | 0.001707 | 0.008455 |
Cytoplasmic Foci | 0.062171 | 0.052555 | 0.062842 | 0.065470 | 0.020035 | 0.038346 | 0.035213 | 0.029425 | 0.044481 | 0.044091 | 0.038083 | 0.038852 |
Eisosomes | 0.000084 | 0.000097 | 0.000094 | 0.000156 | 0.000063 | 0.000073 | 0.000043 | 0.000076 | 0.000075 | 0.000083 | 0.000048 | 0.000097 |
Endoplasmic Reticulum | 0.000801 | 0.000453 | 0.001843 | 0.001318 | 0.000474 | 0.000278 | 0.000286 | 0.000389 | 0.000664 | 0.000348 | 0.000448 | 0.000632 |
Endosome | 0.095802 | 0.098443 | 0.132025 | 0.085690 | 0.053855 | 0.080185 | 0.061048 | 0.045356 | 0.078191 | 0.087567 | 0.068421 | 0.055905 |
Golgi | 0.096231 | 0.093231 | 0.063709 | 0.115771* | 0.051292 | 0.088497 | 0.061133 | 0.066745 | 0.077364 | 0.090411 | 0.061400 | 0.079567 |
Lipid Particles | 0.007675 | 0.005884 | 0.006046 | 0.001525 | 0.005805 | 0.002878 | 0.001124 | 0.000602 | 0.006890 | 0.004093 | 0.001636 | 0.000843 |
Mitochondria | 0.583674* | 0.569247* | 0.412467* | 0.461425* | 0.788251* | 0.686924* | 0.733470* | 0.715512* | 0.669563* | 0.639348* | 0.700128* | 0.649059* |
Mitotic Spindle | 0.012241 | 0.015743 | 0.093917 | 0.067572 | 0.005541 | 0.007454 | 0.033314 | 0.037585 | 0.009428 | 0.010805 | 0.039609 | 0.045428 |
None | 0.001352 | 0.000612 | 0.000293 | 0.000319 | 0.001135 | 0.000310 | 0.000214 | 0.000296 | 0.001261 | 0.000432 | 0.000222 | 0.000302 |
Nuclear Periphery | 0.000421 | 0.000087 | 0.008412 | 0.000176 | 0.000102 | 0.000052 | 0.000415 | 0.000201 | 0.000287 | 0.000066 | 0.001246 | 0.000195 |
Nuclear Periphery Foci | 0.000690 | 0.000351 | 0.004819 | 0.000393 | 0.000405 | 0.000268 | 0.000415 | 0.000381 | 0.000570 | 0.000301 | 0.000873 | 0.000384 |
Nucleolus | 0.001338 | 0.001069 | 0.002970 | 0.000107 | 0.000668 | 0.000410 | 0.000152 | 0.000065 | 0.001057 | 0.000677 | 0.000445 | 0.000076 |
Nucleus | 0.002592 | 0.001097 | 0.002697 | 0.000338 | 0.001010 | 0.000405 | 0.000141 | 0.000122 | 0.001928 | 0.000685 | 0.000407 | 0.000179 |
Peroxisomes | 0.037533 | 0.106657* | 0.042461 | 0.067488 | 0.033399 | 0.064614 | 0.019315 | 0.030702 | 0.035797 | 0.081612 | 0.021719 | 0.040323 |
Vacuole | 0.024946 | 0.008851 | 0.057252 | 0.018341 | 0.008598 | 0.005906 | 0.019777 | 0.015255 | 0.018082 | 0.007097 | 0.023669 | 0.016062 |
Vacuole Periphery | 0.022822 | 0.009659 | 0.061691 | 0.012628 | 0.011833 | 0.007692 | 0.021915 | 0.013131 | 0.018208 | 0.008488 | 0.026047 | 0.012999 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.52 | -4.28 | -8.40 | -7.61 | -6.90 | -2.93 | -0.97 | -7.02 | -5.81 | -6.76 | -6.38 | -4.06 | -11.12 | -9.41 | -10.11 |
Bud Neck | 5.27 | 0.97 | 7.29 | 2.30 | 4.15 | 2.66 | 3.19 | 3.37 | 1.21 | 0.01 | 6.92 | 8.10 | 9.19 | 3.50 | 1.12 |
Bud Site | 0.92 | -1.17 | -7.91 | -8.13 | -4.28 | -0.09 | 0.65 | -9.48 | -9.62 | -10.66 | 2.38 | 5.26 | -9.34 | -11.20 | -13.63 |
Cell Periphery | 0.08 | 0.01 | -0.71 | -0.86 | -0.79 | 2.75 | 3.35 | 2.49 | -1.14 | -4.58 | 2.63 | 4.32 | 2.29 | -0.79 | -5.71 |
Cytoplasm | 3.21 | 9.00 | 0.02 | -2.87 | -7.40 | -0.30 | 1.56 | -5.34 | -5.61 | -9.71 | 3.94 | 8.70 | -0.33 | -5.01 | -11.99 |
Cytoplasmic Foci | 2.83 | -0.11 | -0.70 | -3.04 | -0.34 | -8.79 | -7.58 | -4.84 | 4.07 | 2.74 | 0.21 | 3.04 | 2.82 | 2.74 | -0.33 |
Eisosomes | -2.00 | -1.38 | -6.61 | -4.90 | -4.46 | -2.11 | 5.52 | -2.95 | -0.66 | -9.71 | -1.96 | 9.18 | -5.05 | -2.92 | -12.36 |
Endoplasmic Reticulum | 2.31 | -4.33 | -1.96 | -3.54 | 1.60 | 1.84 | 1.97 | 0.68 | -1.68 | -2.06 | 3.20 | 2.19 | 0.08 | -3.58 | -2.44 |
Endosome | -0.49 | -3.64 | 1.90 | 2.14 | 4.50 | -6.25 | -2.09 | 2.61 | 9.50 | 5.62 | -2.76 | 3.09 | 7.87 | 10.20 | 4.46 |
Golgi | 0.59 | 4.65 | -3.00 | -3.29 | -6.27 | -8.49 | -2.64 | -3.96 | 5.19 | -1.59 | -3.80 | 5.03 | -0.62 | 3.12 | -5.52 |
Lipid Particles | 1.38 | 0.93 | 6.70 | 4.10 | 3.13 | 2.65 | 4.70 | 5.22 | 4.47 | 2.81 | 3.43 | 7.76 | 9.17 | 6.26 | 3.40 |
Mitochondria | 1.07 | 7.81 | 8.17 | 6.37 | -2.03 | 9.09 | 5.64 | 7.34 | -2.59 | 1.97 | 3.42 | -3.50 | 2.51 | -0.96 | 5.71 |
Mitotic Spindle | -1.83 | -7.18 | -10.80 | -9.90 | 2.06 | -1.72 | -12.61 | -13.67 | -13.39 | -1.42 | -1.29 | -13.19 | -16.39 | -15.74 | -1.96 |
None | 2.12 | 3.36 | 3.15 | 1.61 | -0.26 | 1.53 | 1.73 | 1.57 | 0.21 | -2.15 | 2.78 | 3.61 | 3.32 | 1.54 | -2.08 |
Nuclear Periphery | 1.64 | -3.02 | 1.16 | -1.69 | 3.12 | 2.40 | -4.89 | -2.69 | -4.69 | 3.11 | 1.86 | -3.13 | 0.76 | -4.63 | 3.70 |
Nuclear Periphery Foci | 1.64 | -4.41 | 1.28 | -0.47 | 4.81 | 0.42 | -0.06 | 0.08 | -0.47 | 0.23 | 1.55 | -1.65 | 1.06 | -0.56 | 3.31 |
Nucleolus | 0.62 | -1.02 | 4.67 | 2.46 | 1.88 | 0.90 | 2.06 | 3.03 | 2.12 | 1.62 | 1.49 | 2.34 | 5.71 | 3.26 | 2.37 |
Nucleus | 2.08 | -0.16 | 3.03 | 1.35 | 2.55 | 1.68 | 2.56 | 2.58 | 1.77 | 0.21 | 2.99 | 3.91 | 4.37 | 2.52 | 1.45 |
Peroxisomes | -11.00 | -0.97 | -5.53 | 5.10 | -3.04 | -6.60 | 4.37 | 0.75 | 7.90 | -4.57 | -12.29 | 6.20 | -1.70 | 10.70 | -7.50 |
Vacuole | 9.68 | -5.96 | 2.53 | -4.71 | 6.89 | 2.37 | -7.91 | -4.92 | -9.16 | 3.14 | 10.48 | -4.06 | 1.23 | -9.63 | 5.39 |
Vacuole Periphery | 8.41 | -5.91 | 5.45 | -1.60 | 7.38 | 3.54 | -6.63 | -1.02 | -5.27 | 6.20 | 10.01 | -5.30 | 4.93 | -4.89 | 9.01 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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