Standard name
Description Mitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0.06 0.05 0 0 0.05 0 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.09 0.11 0.1 0.12 0.06 0 0 0 0 0 0 0 0.25 0.17 0.2 0.2 0.16 0.11 0.1 0.13 0.1 0.12 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.1 0 0 0 0 0.06 0.06
Mitochondria 0.81 0.78 0.68 0.81 0.72 0.84 0.85 0.8 0.92 0.92 0.95 0.92 0.83 0.59 0.57 0.05 0.1 0.11 0.67 0.68 0.66 0.62 0.48 0.52
Nucleus 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0.13 0.12 0.13 0.13 0.08 0 0 0 0 0 0 0 0.16 0.35 0.22 0.32 0.24 0.25 0.06 0 0.09 0.11 0.16 0.12
SpindlePole 0.09 0.07 0.22 0.1 0.11 0.06 0.08 0.07 0 0 0 0.05 0.08 0.15 0.11 0.37 0.42 0.33 0.06 0 0.05 0 0 0.05
Vac/Vac Membrane 0.12 0.19 0.1 0.07 0.12 0.13 0.07 0.09 0.05 0.06 0 0 0.05 0.07 0.07 0.13 0.16 0.17 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 5 0 0 0 0 1 2 1 0 0 1 3 1 0 1 3 1 8 14 9
Bud 8 6 16 9 10 7 19 10 16 22 15 7 13 7 8 9 8 6 1 1 4 2 2 6
Bud Neck 1 0 1 0 0 0 0 1 1 1 1 0 0 0 2 2 3 0 1 0 0 1 5 4
Bud Site 9 4 2 2 0 1 4 5 3 6 4 3 2 1 1 5 1 6
Cell Periphery 2 6 6 3 3 4 1 2 2 3 3 0 3 4 3 16 13 6 0 0 0 0 0 0
Cytoplasm 4 7 7 6 10 5 11 12 7 10 4 5 9 7 18 21 11 7 0 0 0 0 0 0
Endoplasmic Reticulum 1 1 0 0 2 1 1 1 0 2 0 0 0 0 4 9 7 9 0 0 0 1 2 4
Endosome 24 22 29 18 27 16 10 15 2 2 0 3 12 63 60 73 49 39 31 26 36 23 34 27
Golgi 0 3 1 0 8 2 0 1 0 0 0 0 2 2 2 24 12 24 12 11 7 7 18 18
Mitochondria 223 197 175 142 162 224 318 271 263 292 199 181 259 151 199 19 25 28 196 178 190 142 138 148
Nucleus 3 3 2 2 4 10 21 19 9 8 5 3 0 1 3 8 3 9 0 0 0 0 1 1
Nuclear Periphery 4 2 0 0 5 6 6 12 9 9 7 5 3 0 1 2 2 0 1 2 0 1 3 2
Nucleolus 17 6 3 0 1 10 11 10 10 7 7 5 13 3 13 14 12 11 1 0 0 0 2 0
Peroxisomes 36 30 34 23 18 7 11 6 2 1 3 1 49 91 76 117 59 62 19 11 26 24 46 33
SpindlePole 25 18 56 18 25 15 30 24 10 6 7 10 25 39 38 134 104 82 17 10 15 5 6 13
Vac/Vac Membrane 33 48 25 13 27 35 28 32 14 18 4 6 15 17 26 48 40 43 4 13 3 5 4 8
Unique Cell Count 274 253 259 176 224 268 376 339 287 316 209 196 313 257 347 367 248 251 295 263 291 229 288 286
Labelled Cell Count 390 354 358 236 307 343 471 421 348 388 261 230 405 386 455 504 350 332 295 263 291 229 288 286


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.0 6.0 5.4 6.3 5.1 5.3 5.5 4.9 4.4 4.0 4.5 5.8 6.2 6.2 8.2 8.7 8.4 5.8 5.8 6.1
Std Deviation (1e-4) 1.0 1.1 0.9 0.8 1.3 1.1 1.2 1.2 1.5 1.0 0.9 0.8 1.2 1.6 1.5 1.7 2.0 1.9 1.5 1.9 1.6
Intensity Change (Log2) -0.15 0.08 -0.23 -0.17 -0.11 -0.29 -0.43 -0.56 -0.41 -0.03 0.06 0.05 0.46 0.54 0.5 -0.05 -0.03 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.5 -0.8 -2.0 -0.6 -1.9 -0.3 0 0 0 -1.1 -1.9 -2.4 -2.3 -1.6 -2.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.4 1.7 0.1
Cytoplasm 0.4 1.0 -0.6 0.2 0.6 -0.2 0.3 0 -0.1 0.1 0 1.5 1.8 1.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.3 0.3 -2.1 -4.4 -3.1 -5.3 0 0 0 -3.4 4.0 2.1 2.9 2.7 1.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.9 3.2 4.8
Mitochondria 3.0 1.1 4.3 5.1 3.4 7.1 0 0 0 4.2 -2.1 -2.6 -16.6 -13.2 -13.0
Nucleus 0 0 2.3 3.2 3.2 2.0 0 0 0 0 0 0 0 0 2.2
Nuclear Periphery 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.9 1.5 1.5 1.8 0 0 0 2.2 0 2.0 2.0 2.4 2.2
Peroxisomes 0 -1.8 -4.5 -4.9 -5.5 -5.8 0 0 0 0.9 5.9 2.8 5.4 3.1 3.3
SpindlePole -3.1 -3.1 -5.4 -4.9 -5.2 -6.5 0 0 0 -4.7 -1.9 -3.6 4.0 4.9 2.8
Vacuole -0.8 0.8 1.2 -1.0 -0.1 -2.2 -1.8 -3.5 -2.8 -2.3 -1.3 -0.9 1.3 2.2 2.5