Standard name
Human Ortholog
Description Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.07 0.19 0.85 0.74 0.65 0.53 0.6 0.53 0.5 0.43 0.55 0.2 0.42 0.72 0.32 0.24 0.28 0 0 0.16 0.4 0.63 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.16 0.12 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.29 0.1 0.47 0.2 0.27 0.51 0.66 0.55 0.64 0.68 0.73 0.63 0.05 0 0 0.13 0.05 0.07 0 0 0.07 0 0 0
Nucleus 0.77 0.73 0.27 0 0 0 0 0 0 0.05 0 0 0.68 0.25 0 0.3 0.47 0.53 0.66 0.69 0.32 0.2 0.08 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.08 0.08 0.06 0.07
Vac/Vac Membrane 0.07 0.14 0.16 0 0.09 0 0 0 0 0 0 0 0.1 0.29 0.26 0.2 0.18 0.17 0.08 0.13 0.2 0.14 0.09 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 1
Bud 0 0 4 4 0 3 1 7 11 5 2 2 0 0 0 0 0 0 2 5 4 3 7 18
Bud Neck 0 0 2 0 1 0 0 2 1 2 1 0 0 0 0 0 1 0 0 1 3 2 7 8
Bud Site 0 1 0 0 0 1 4 1 2 1 0 2 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 19 35 147 138 96 138 203 152 182 57 77 40 105 221 165 78 73 1 3 42 69 219 99
Endoplasmic Reticulum 0 0 2 0 0 1 0 0 0 2 1 0 0 1 0 39 17 13 0 0 1 0 2 4
Endosome 2 3 1 0 2 0 0 1 0 0 0 0 3 19 30 82 40 11 4 8 8 6 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 1 0 0 0
Mitochondria 32 28 84 34 51 75 172 188 185 246 96 89 10 10 4 69 15 17 2 6 19 4 12 7
Nucleus 86 196 48 1 1 3 6 12 8 17 5 5 135 64 9 155 155 136 82 220 83 35 26 28
Nuclear Periphery 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 0
Nucleolus 14 13 0 0 2 1 0 0 0 0 0 1 2 0 1 5 1 1 11 13 7 4 3 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0
SpindlePole 1 0 2 1 1 0 1 5 5 0 0 0 0 0 1 27 12 1 4 15 20 14 19 18
Vac/Vac Membrane 8 37 28 4 16 5 8 11 10 5 1 3 20 74 79 103 58 43 9 40 51 24 29 46
Unique Cell Count 111 269 180 172 186 147 261 341 287 362 132 141 199 253 305 516 327 257 124 322 261 177 348 252
Labelled Cell Count 148 297 206 191 212 185 332 431 374 461 163 179 211 274 345 647 378 296 124 322 261 177 348 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 3.8 3.3 3.6 3.1 2.6 3.0 2.9 2.6 2.6 2.6 5.6 4.9 4.8 5.6 6.6 6.5 4.4 4.4 5.6
Std Deviation (1e-4) 0.7 1.0 0.6 1.0 1.3 1.1 1.0 0.8 0.9 0.6 0.5 0.8 1.2 1.0 1.5 1.5 1.5 1.6 1.2 1.1 1.9
Intensity Change (Log2) – – – -0.19 -0.08 -0.3 -0.56 -0.36 -0.42 -0.57 -0.54 -0.57 0.55 0.37 0.33 0.55 0.79 0.78 0.22 0.2 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12.4 10.5 8.4 7.1 8.7 7.2 6.9 4.5 6.6 0.2 4.8 11.3 3.2 1.1 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 2.3 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 3.4 0 5.5 4.6 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -9.4 -10.6 0 -9.3 -11.4 -9.8
Nucleus -7.1 -7.3 -6.1 -7.7 -7.9 -7.7 -7.4 -5.3 -5.5 8.0 -0.3 -7.8 0.9 4.6 5.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Vacuole -4.3 -2.0 -3.6 -4.7 -5.1 -4.6 -6.5 -4.4 -4.0 -1.6 3.3 2.7 1.3 0.6 0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.1013 0.0002 0.0253 0.0003 0.0001 0.0013 0.0821 0.0188 0.0035 0.0007 0.1071 0.0023
Bud – – – – – – 0.0005 0.0001 0.001 0 0 0.0007 0.0009 0.0007 0.0006 0.0002 0.0003 0.0007
Bud Neck – – – – – – 0.0009 0.0006 0.0024 0.0002 0.0004 0.0055 0.0272 0.0004 0.0007 0.0015 0.0099 0.0055
Bud Periphery – – – – – – 0.0004 0.0001 0.0014 0 0 0.0019 0.0069 0.0007 0.001 0.0007 0.0007 0.0022
Bud Site – – – – – – 0.0031 0.0007 0.0037 0.0001 0.0001 0.0033 0.0024 0.0102 0.0068 0.0004 0.0026 0.0006
Cell Periphery – – – – – – 0.0001 0 0.0002 0 0 0.0002 0.0031 0.0001 0.0003 0.0001 0.0023 0.0001
Cytoplasm – – – – – – 0.0231 0.026 0.0156 0.0345 0.0521 0.0174 0.0062 0.027 0.0227 0.0245 0.0353 0.0196
Cytoplasmic Foci – – – – – – 0.0667 0.0002 0.008 0.0003 0.0062 0.0091 0.0268 0.0059 0.0075 0.0009 0.0021 0.0036
Eisosomes – – – – – – 0.0003 0 0.0001 0 0 0 0.0003 0.0002 0.0002 0.0001 0.0007 0.0001
Endoplasmic Reticulum – – – – – – 0.0021 0.0009 0.0007 0.0006 0.0039 0.0008 0.0023 0.0018 0.0008 0.0012 0.0241 0.0008
Endosome – – – – – – 0.0277 0.0005 0.0126 0.0005 0.0086 0.0087 0.006 0.0197 0.0045 0.0057 0.0051 0.0037
Golgi – – – – – – 0.0116 0 0.0067 0 0.0001 0.0019 0.0073 0.0096 0.0033 0.0013 0.0017 0.0024
Lipid Particles – – – – – – 0.0326 0 0.0085 0 0.0021 0.0025 0.0248 0.0022 0.0037 0.001 0.0026 0.0012
Mitochondria – – – – – – 0.0027 0.0005 0.0116 0.0003 0.0002 0.002 0.0071 0.0043 0.0107 0.034 0.0017 0.0036
None – – – – – – 0.0093 0.0518 0.0064 0.0548 0.0614 0.0164 0.0018 0.0101 0.0421 0.0572 0.0259 0.0185
Nuclear Periphery – – – – – – 0.0068 0.0181 0.0109 0.0092 0.0127 0.0071 0.0047 0.0116 0.0143 0.0053 0.0043 0.0059
Nucleolus – – – – – – 0.0043 0.0055 0.0068 0.0021 0.0059 0.0134 0.0077 0.0053 0.0046 0.0062 0.0186 0.0105
Nucleus – – – – – – 0.5876 0.8604 0.7924 0.889 0.785 0.8762 0.6627 0.8635 0.8301 0.8401 0.7456 0.8952
Peroxisomes – – – – – – 0.0434 0 0.0125 0 0.0001 0.0025 0.0577 0.0012 0.0183 0.0002 0.0014 0.0006
Punctate Nuclear – – – – – – 0.0744 0.0331 0.0708 0.0077 0.0598 0.0255 0.0538 0.0045 0.023 0.014 0.0039 0.0213
Vacuole – – – – – – 0.0009 0.0008 0.0015 0.0002 0.0008 0.0031 0.0069 0.0017 0.001 0.0024 0.0036 0.0013
Vacuole Periphery – – – – – – 0.0004 0.0002 0.0006 0.0001 0.0002 0.0005 0.0011 0.0006 0.0003 0.0023 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.2685 15.7723 13.0542 14.2051 18.4765 12.8863 17.3274 21.5268 17.7196 21.9353
Translational Efficiency 1.3876 1.5501 1.2204 1.0984 1.2085 1.7698 1.2988 0.9401 1.121 1.2357

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1221 1582 110 2045 99 2194 334 1116 1320 3776 444 3161

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.16 721.21 954.61 976.18 780.92 779.51 995.73 1304.34 716.39 755.08 985.54 1092.04
Standard Deviation 72.14 144.73 104.00 136.32 87.52 101.51 105.94 200.25 75.67 124.86 106.95 225.34
Intensity Change Log 2 — 0.020245 0.424737 0.456973 — -0.002607 0.350580 0.740073 — 0.008330 0.386401 0.612038

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000168 0.000340 0.000988 0.000959 0.000418 0.000249 0.000891 0.000301 0.000187 0.000287 0.000915 0.000726
Bud Neck 0.002259 0.008632 0.002177 0.020776 0.000791 0.007458 0.009556 0.028485 0.002149 0.007950 0.007728 0.023497
Bud Site 0.001371 0.002694 0.000932 0.016948 0.000460 0.002557 0.002645 0.017033 0.001303 0.002615 0.002220 0.016978
Cell Periphery 0.000160 0.000164 0.000116 0.000175 0.000188 0.000079 0.000140 0.000239 0.000162 0.000115 0.000134 0.000197
Cytoplasm 0.131866* 0.153078* 0.030733 0.041691 0.038411 0.091791 0.028659 0.220129* 0.124857* 0.117468* 0.029173 0.104689*
Cytoplasmic Foci 0.022448 0.022694 0.005579 0.001046 0.037573 0.012994 0.000377 0.001642 0.023582 0.017058 0.001666 0.001256
Eisosomes 0.000046 0.000043 0.000082 0.000022 0.000121 0.000028 0.000054 0.000016 0.000051 0.000034 0.000061 0.000020
Endoplasmic Reticulum 0.001148 0.003569 0.012326 0.006964 0.003167 0.002284 0.009310 0.001069 0.001299 0.002822 0.010058 0.004882
Endosome 0.001185 0.003237 0.001221 0.001481 0.002019 0.002653 0.001030 0.001941 0.001247 0.002898 0.001077 0.001644
Golgi 0.000254 0.001244 0.000082 0.002243 0.000987 0.001638 0.000086 0.000434 0.000309 0.001473 0.000085 0.001604
Lipid Particles 0.005121 0.004843 0.001772 0.000233 0.009453 0.003125 0.000773 0.000232 0.005446 0.003845 0.001020 0.000233
Mitochondria 0.002012 0.005517 0.000552 0.004264 0.001382 0.010791 0.000919 0.005491 0.001965 0.008581 0.000828 0.004697
Mitotic Spindle 0.001545 0.005773 0.000053 0.024611 0.006520 0.013487 0.001265 0.019990 0.001918 0.010255 0.000964 0.022979
None 0.018073 0.039323 0.000925 0.005243 0.008421 0.008362 0.008018 0.023742 0.017349 0.021333 0.006261 0.011775
Nuclear Periphery 0.001198 0.002854 0.001098 0.002385 0.001041 0.003863 0.001642 0.001344 0.001186 0.003440 0.001507 0.002017
Nuclear Periphery Foci 0.000933 0.002700 0.000720 0.000626 0.002093 0.001044 0.000711 0.000312 0.001020 0.001738 0.000713 0.000515
Nucleolus 0.012508 0.015425 0.002199 0.001080 0.014646 0.010541 0.002318 0.001210 0.012668 0.012587 0.002288 0.001126
Nucleus 0.792218* 0.716596* 0.916332* 0.857841* 0.859678* 0.818833* 0.912399* 0.663845* 0.797277* 0.776000* 0.913373* 0.789350*
Peroxisomes 0.001636 0.002749 0.000498 0.000149 0.004327 0.002319 0.000225 0.000233 0.001838 0.002499 0.000293 0.000178
Vacuole 0.003670 0.007562 0.021463 0.010086 0.008069 0.005147 0.018680 0.010283 0.004000 0.006159 0.019369 0.010155
Vacuole Periphery 0.000182 0.000963 0.000151 0.001179 0.000234 0.000755 0.000303 0.002031 0.000186 0.000842 0.000265 0.001480

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.57 -5.56 -10.25 -7.83 1.37 2.26 -3.92 2.09 -0.17 8.30 -2.45 -10.14 -8.66 -8.34 4.24
Bud Neck -7.54 -1.69 -18.48 -11.75 -16.45 -9.64 -4.12 -11.55 -8.47 -6.79 -9.40 -3.60 -20.32 -14.88 -11.09
Bud Site -2.87 -0.71 -6.44 -5.42 -6.19 -3.74 -4.89 -6.35 -5.21 -4.94 -2.90 -2.83 -8.54 -7.47 -7.34
Cell Periphery 0.02 0.60 1.14 1.15 0.07 4.27 2.46 1.50 -4.88 -1.46 4.73 2.07 1.12 -3.70 -1.10
Cytoplasm -2.37 9.58 16.69 21.04 -0.60 -7.50 1.91 -15.70 -11.98 -20.51 1.43 15.59 6.43 7.18 -12.97
Cytoplasmic Foci 0.16 5.46 12.66 13.99 2.14 3.06 4.37 4.29 11.91 -3.02 3.71 11.87 13.17 18.58 1.62
Eisosomes 0.48 -4.01 5.97 6.61 5.63 5.49 3.72 6.24 8.31 9.42 5.44 -3.01 9.48 8.43 9.70
Endoplasmic Reticulum -8.95 -9.18 -23.86 -11.44 4.31 2.12 -7.40 3.51 6.84 13.00 -9.31 -15.57 -19.98 -12.05 9.44
Endosome -4.87 -1.33 2.00 5.77 2.10 0.28 1.98 1.95 2.92 0.25 -4.99 -0.11 2.00 6.18 2.01
Golgi -3.92 3.35 -1.26 0.84 -1.67 -0.49 1.46 1.21 3.58 -1.37 -4.67 3.43 -0.97 2.23 -1.88
Lipid Particles 0.83 4.50 7.29 9.64 4.96 3.68 5.00 5.32 10.10 4.88 2.67 6.95 8.30 13.95 7.07
Mitochondria -3.18 1.76 -1.97 2.07 -8.12 -7.26 1.50 -3.35 4.34 -4.13 -6.13 1.40 -2.42 5.27 -7.91
Mitotic Spindle -3.22 1.79 -7.17 -4.98 -8.32 -0.39 0.76 -1.03 -1.57 -3.95 -5.58 -0.69 -8.26 -3.87 -6.21
None -5.58 10.09 7.73 11.21 -13.10 0.05 1.19 -4.43 -7.10 -6.65 -1.35 6.72 4.63 7.71 -4.33
Nuclear Periphery -7.95 -1.34 -10.19 -2.53 -7.34 -7.91 -5.08 -2.03 5.44 2.70 -10.87 -5.64 -9.42 1.91 -2.11
Nuclear Periphery Foci -3.62 -0.07 2.70 4.88 1.70 1.35 1.50 1.97 4.29 5.07 -2.05 1.01 3.96 6.05 4.00
Nucleolus -1.56 9.96 10.68 13.50 1.34 1.28 3.52 3.37 10.71 -1.11 0.34 10.95 10.93 18.14 -0.36
Nucleus 6.68 -5.89 -8.65 -16.42 1.91 0.41 -2.16 4.42 9.80 12.80 2.02 -8.53 -2.33 -5.71 7.79
Peroxisomes -2.38 3.32 5.22 8.28 2.21 1.94 3.56 3.57 7.76 0.07 -1.48 5.53 6.03 11.39 2.35
Vacuole -3.83 -4.05 -10.32 -7.04 1.80 1.52 -3.69 0.23 -5.16 5.19 -2.24 -7.58 -9.91 -9.65 4.85
Vacuole Periphery -3.98 0.06 -1.97 1.65 -1.97 -3.45 0.97 -1.46 -0.70 -1.57 -5.69 -0.03 -2.26 0.24 -2.22
Standard name
Human Ortholog
Description Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Stp2

Stp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available