Standard name
Description Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0.42 0.7 0.97 0.74 0.69 0.55 0.32 0.24 0.96 0.97 0.99 0.84 0.89 0.38 0.5 0.46 0.85 0.86 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0.05 0 0
Endosome 0.1 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.1 0 0.09 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.4 0.55 0.43 0.68 0.73 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.12 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.07 0 0 0 0 0.07 0.08 0 0 0 0 0 0.06 0 0.06 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.11 0 0 0
Vac/Vac Membrane 0.34 0.22 0 0.06 0 0.07 0.14 0.1 0 0 0 0.16 0.11 0.1 0.22 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 3 3 4 0 0 0 0 0 6 0 3 0 0 0
Bud Neck 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 1 0 0 0 0 0
Cell Periphery 0 0 1 1 1 0 0 0 0 6 10 1 0 0 0 0 0 0 0
Cytoplasm 44 19 28 46 35 48 18 12 55 122 126 21 16 60 30 28 58 61 91
Endoplasmic Reticulum 0 0 0 0 0 1 1 0 1 3 0 0 2 3 0 0 3 1 3
Endosome 11 0 0 0 0 3 0 0 1 0 0 2 1 16 2 5 0 0 1
Golgi 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 1 0
Mitochondria 11 0 0 25 28 38 38 36 0 0 0 0 0 2 0 1 1 1 2
Nucleus 5 0 0 0 0 3 2 1 0 1 0 0 0 17 7 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0
Nucleolus 21 2 0 1 2 2 4 4 0 0 1 0 0 10 1 3 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 1 1 0 0 0 0 0 12 1 6 1 0 0
Vac/Vac Membrane 36 6 0 4 0 6 8 5 1 2 0 4 2 16 13 2 1 1 2
Unique Cell Count 105 27 29 62 51 88 56 49 57 126 127 25 18 157 61 61 69 71 106
Labelled Cell Count 130 29 29 77 67 109 75 64 58 135 137 28 21 157 61 61 69 71 106


Cytoplasm, Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 14.8 9.1 5.8 5.4 5.9 5.2 5.7 8.8 9.5 9.4 22.3 9.8 9.3 9.2 10.0
Std Deviation (1e-4) 1.7 5.3 2.0 1.2 1.1 1.6 1.4 1.5 1.7 1.6 1.9 35.9 1.7 2.1 1.9 2.2
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl27a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available