Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | – | – | 0 | 0 | 0 | 3 | – | 3 | 4 | – | 0 | 0 | 0 | 0 | 0 | – | 6 | 0 | 3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | – | 0 | 0 | 0 | 2 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | – | 0 | 0 | 0 | 1 | – | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | – | 1 | 1 | 1 | 0 | – | 0 | 0 | – | 0 | 6 | 10 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 44 | 19 | – | – | 28 | 46 | 35 | 48 | – | 18 | 12 | – | 55 | 122 | 126 | 21 | 16 | – | 60 | 30 | 28 | 58 | 61 | 91 |
Endoplasmic Reticulum | 0 | 0 | – | – | 0 | 0 | 0 | 1 | – | 1 | 0 | – | 1 | 3 | 0 | 0 | 2 | – | 3 | 0 | 0 | 3 | 1 | 3 |
Endosome | 11 | 0 | – | – | 0 | 0 | 0 | 3 | – | 0 | 0 | – | 1 | 0 | 0 | 2 | 1 | – | 16 | 2 | 5 | 0 | 0 | 1 |
Golgi | 0 | 1 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 11 | 0 | – | – | 0 | 25 | 28 | 38 | – | 38 | 36 | – | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 1 | 1 | 2 |
Nucleus | 5 | 0 | – | – | 0 | 0 | 0 | 3 | – | 2 | 1 | – | 0 | 1 | 0 | 0 | 0 | – | 17 | 7 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 21 | 2 | – | – | 0 | 1 | 2 | 2 | – | 4 | 4 | – | 0 | 0 | 1 | 0 | 0 | – | 10 | 1 | 3 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | – | 0 | 0 | 1 | 2 | – | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | – | 12 | 1 | 6 | 1 | 0 | 0 |
Vac/Vac Membrane | 36 | 6 | – | – | 0 | 4 | 0 | 6 | – | 8 | 5 | – | 1 | 2 | 0 | 4 | 2 | – | 16 | 13 | 2 | 1 | 1 | 2 |
Unique Cell Count | 105 | 27 | 29 | 62 | 51 | 88 | 56 | 49 | 57 | 126 | 127 | 25 | 18 | 157 | 61 | 61 | 69 | 71 | 106 | |||||
Labelled Cell Count | 130 | 29 | 29 | 77 | 67 | 109 | 75 | 64 | 58 | 135 | 137 | 28 | 21 | 157 | 61 | 61 | 69 | 71 | 106 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 14.8 | – | – | 9.1 | 5.8 | 5.4 | 5.9 | – | 5.2 | 5.7 | – | 8.8 | 9.5 | 9.4 | 22.3 | 9.8 | – | 9.3 | 9.2 | 10.0 |
Std Deviation (1e-4) | 1.7 | 5.3 | – | – | 2.0 | 1.2 | 1.1 | 1.6 | – | 1.4 | 1.5 | – | 1.7 | 1.6 | 1.9 | 35.9 | 1.7 | – | 2.1 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2504.8086 | 2426.3414 | 2747.8678 | 2364.5553 | 2718.5111 | 2232.4192 | 2126.8964 | 1958.7816 | 2178.3014 | 1952.276 |
Translational Efficiency | 1.6633 | 1.7097 | 1.5986 | 1.4843 | 1.7106 | 1.9808 | 1.7353 | 2.1429 | 1.7211 | 2.1266 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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