Standard name
Description Subunit of protein N-terminal acetyltransferase NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many proteins to influence telomeric silencing, cell cycle, heat-shock resistance, mating, sporulation, early stages of mitophagy; protein abundance increases under DNA replication stress; mutations in human homolog X-linked NAA10 lead to Ogden syndrome (S37P) and intellectual disability (R116W); expression of human NAA10 and NAA15 can complement ard1 nat1 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0.05 0.07 0.07 0.13 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 0.92 0.89 0.83 0.79 – 0.72 0.66 0.71 0.69 0.99 0.99 0.97 0.95 0.93 0.86 0.93 0.95 0.95 0.91 0.9 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.08 – 0.24 0.28 0.27 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.07 0.13 – 0.09 0.1 0.09 0.07 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 3 1 4 – 6 8 8 11 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 1 0 0 0 5 2 – 8 5 7 11 0 0 0 0 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 11 9 4 15 14 18 – 19 29 18 29 6 3 14 8 10 14 0 0 0 0 0 0
Cytoplasm 193 379 385 272 311 371 391 – 411 429 408 426 229 247 303 114 130 96 190 368 395 200 277 252
Endoplasmic Reticulum 2 7 9 8 14 9 14 – 15 13 6 22 4 3 6 4 3 5 3 2 5 10 18 11
Endosome 0 0 0 0 0 2 1 – 3 10 1 4 0 0 0 0 0 1 1 0 0 1 0 1
Golgi 1 0 0 1 3 0 1 – 0 1 1 1 0 0 0 0 0 0 1 0 0 0 1 1
Mitochondria 0 0 1 4 3 85 37 – 136 184 155 167 0 1 2 1 2 0 1 0 4 1 2 2
Nucleus 1 3 2 0 4 2 4 – 4 5 0 3 0 0 0 1 2 2 0 3 2 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 1 1 0 1 0 0 0 0 0 0 2 0 0 1 1 1
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 1 – 0 1 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 3 6 16 15 32 62 – 51 63 50 41 0 0 4 3 6 8 0 6 5 2 1 6
Unique Cell Count 198 391 401 296 348 449 492 573 648 574 620 232 250 311 120 140 111 205 388 417 221 308 282
Labelled Cell Count 202 406 412 305 369 521 535 654 750 656 721 239 254 329 131 153 126 205 388 417 221 308 282


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.6 12.0 11.4 10.7 10.2 6.9 8.2 – 7.3 7.2 7.2 7.1 13.2 11.6 13.2 17.5 18.1 17.7 12.2 12.9 13.0
Std Deviation (1e-4) 2.3 2.1 1.6 1.3 1.6 1.5 1.6 – 1.3 1.1 1.1 1.2 2.2 1.6 2.0 3.8 3.8 3.9 2.5 2.2 2.7
Intensity Change (Log2) – – – -0.09 -0.16 -0.72 -0.47 – -0.65 -0.66 -0.66 -0.69 0.21 0.03 0.21 0.62 0.67 0.64 0.1 0.17 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 1.6 0.8 1.2 – 1.0 1.9 0.8 2.0 0.3 0 1.7 0 0 0
Cytoplasm -2.3 -3.5 -6.2 -7.3 – -9.7 -11.3 -9.8 -10.5 1.9 2.1 1.0 -0.5 -1.5 -3.7
Endoplasmic Reticulum 0.4 1.4 -0.2 0.6 – 0.4 -0.3 -1.5 1.2 0 0 -0.3 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 9.0 5.4 – 10.4 11.6 11.2 11.2 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.9 2.3 4.0 6.2 – 4.8 5.2 4.8 3.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0647 7.7634 6.9088 5.924 5.6223 7.1159 6.2399 10.1429 9.3233 6.9686 8.7559 8.8344 12.1675 13.9279 13.0839 12.0437 12.7479 13.2297
Actin 0.0204 0.0003 0.0079 0.0128 0.0065 0.0005 0.0228 0.0004 0.0025 0.0067 0.0145 0.0031 0.0004 0.0005 0.0003 0.0001 0.0002 0.0005
Bud 0.0006 0.0003 0.0014 0.0006 0.0019 0.0004 0.0008 0.0004 0.0004 0.0008 0.0042 0.0003 0.0003 0.0004 0.0003 0.0016 0.0003 0.0003
Bud Neck 0.0011 0.0002 0.0004 0.0008 0.0009 0.0008 0.0011 0.0003 0.0003 0.0005 0.0026 0.0007 0.0003 0.0003 0.0003 0.0006 0.0004 0.0008
Bud Periphery 0.0006 0 0.0008 0.0003 0.0019 0.0001 0.0008 0.0001 0.0001 0.0006 0.0038 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001
Bud Site 0.0026 0.0003 0.0018 0.0007 0.0014 0.0001 0.0022 0.0013 0.0005 0.0008 0.0105 0.0002 0.0003 0.0043 0.0003 0.0003 0.0002 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0001 0.0001 0 0.0002
Cytoplasm 0.7357 0.9476 0.8534 0.8569 0.7747 0.8772 0.6376 0.9552 0.8859 0.702 0.8312 0.847 0.8777 0.9459 0.9236 0.9031 0.9063 0.9125
Cytoplasmic Foci 0.0206 0.0076 0.0208 0.0123 0.0296 0.0163 0.0585 0.0076 0.0193 0.0499 0.035 0.0264 0.0108 0.0087 0.0106 0.0134 0.0116 0.0142
Eisosomes 0.0001 0 0 0.0001 0.0001 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0396 0.0063 0.0103 0.0099 0.0051 0.0183 0.0369 0.0064 0.0095 0.0084 0.0024 0.0138 0.0487 0.008 0.0178 0.0067 0.0219 0.0192
Endosome 0.0792 0.0221 0.0629 0.042 0.0997 0.0653 0.1007 0.0129 0.0479 0.0872 0.0416 0.0684 0.0331 0.0154 0.0312 0.0409 0.0327 0.0324
Golgi 0.0146 0.0014 0.0077 0.0076 0.0193 0.0051 0.0248 0.0021 0.005 0.0363 0.0247 0.0073 0.0027 0.0017 0.0016 0.0007 0.0016 0.0041
Lipid Particles 0.0056 0 0.0029 0.0127 0.0152 0.0001 0.0266 0.0002 0.0035 0.0041 0.0008 0.0054 0.0002 0.0002 0.0001 0 0.0001 0.0001
Mitochondria 0.026 0.0008 0.0078 0.0073 0.0176 0.0048 0.0194 0.007 0.0015 0.0661 0.0119 0.0021 0.0012 0.0007 0.0005 0.0003 0.0006 0.0037
None 0.0063 0.0007 0.0017 0.0007 0.0008 0.0007 0.0067 0.0009 0.001 0.0028 0.0005 0.0008 0.007 0.0066 0.0014 0.0009 0.0009 0.0054
Nuclear Periphery 0.0125 0.0038 0.0027 0.0059 0.0013 0.0008 0.0213 0.0007 0.0014 0.0034 0.0008 0.0064 0.0032 0.0005 0.0016 0.0016 0.002 0.0008
Nucleolus 0.0003 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0
Nucleus 0.0034 0.0013 0.0013 0.0019 0.0011 0.0008 0.0053 0.0008 0.0008 0.0015 0.0015 0.0013 0.0015 0.001 0.0011 0.0017 0.0014 0.0008
Peroxisomes 0.0033 0 0.0023 0.0113 0.0117 0.0001 0.0174 0.0001 0.0096 0.003 0.003 0.0075 0 0.0001 0 0 0 0.0001
Punctate Nuclear 0.004 0.0001 0.0009 0.0012 0.0005 0.0001 0.0035 0.0001 0.0002 0.0039 0.0003 0.0012 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0154 0.0053 0.0095 0.0112 0.0068 0.0061 0.009 0.003 0.0081 0.0178 0.0083 0.0051 0.0099 0.0045 0.0073 0.0231 0.0169 0.0034
Vacuole Periphery 0.0078 0.0018 0.0033 0.0034 0.0035 0.0026 0.0039 0.0005 0.0021 0.0036 0.002 0.0028 0.0018 0.0007 0.0017 0.0046 0.0029 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.3292 46.7738 63.2913 64.4283 74.8228 59.9846 58.4206 53.0427 58.071 68.8896
Translational Efficiency 1.7115 2.4152 1.5317 1.7368 1.5998 2.029 1.816 1.4189 1.3991 1.5948

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘