Standard name
Human Ortholog
Description Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.95 0.97 1.0 0.97 0.98 0.95 0.91 – 0.85 0.85 0.55 0.75 0.99 0.99 1.0 0.96 0.95 – 0.8 0.82 0.71 0.84 0.86 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0.1 0.09 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0 0.08 – 0.2 0.18 0.47 0.25 0 0 0 0 0 – 0.11 0.07 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0.05 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 1 0 1 0 0 0 – 0 0 2 1 2 4
Bud 0 0 0 0 0 0 1 – 5 3 4 9 0 0 0 0 2 – 0 0 0 0 2 7
Bud Neck 1 2 3 0 5 7 14 – 6 14 4 8 1 0 1 1 4 – 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 – – – – – – –
Cell Periphery 0 1 1 1 1 0 0 – 1 1 0 0 4 0 9 9 6 – 1 0 0 0 1 2
Cytoplasm 147 156 44 115 218 203 280 – 217 290 85 163 158 264 349 349 365 – 134 133 39 43 203 235
Endoplasmic Reticulum 2 2 0 1 2 0 1 – 0 0 8 2 0 1 2 35 35 – 0 0 0 0 0 1
Endosome 0 1 0 0 0 0 0 – 2 0 0 0 1 0 0 2 4 – 2 2 1 1 0 0
Golgi 0 1 0 0 0 1 0 – 0 0 0 0 0 0 0 1 3 – 0 0 0 0 0 0
Mitochondria 15 0 0 2 2 8 24 – 50 62 73 54 0 0 0 8 6 – 18 11 7 1 6 5
Nucleus 0 0 0 1 0 1 0 – 2 2 0 1 1 0 0 3 3 – 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 1 0 0 – 0 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 1 9 – 12 8 4 11 0 0 0 2 3 – 1 2 1 0 2 3
Vac/Vac Membrane 0 0 0 0 1 1 1 – 2 1 1 1 0 0 0 5 4 – 4 5 1 1 3 2
Unique Cell Count 154 161 44 118 223 213 307 256 341 155 218 159 266 350 364 383 169 163 56 51 236 273
Labelled Cell Count 165 164 48 120 231 222 330 299 381 179 251 165 266 362 415 437 169 163 56 51 236 273


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.1 4.5 4.9 4.9 3.9 4.0 – 3.5 3.6 3.0 3.3 4.9 4.9 4.8 5.3 5.5 4.3 – 4.6 4.8
Std Deviation (1e-4) 0.5 0.7 0.7 1.9 1.3 1.0 1.2 – 1.2 1.1 0.5 0.7 1.0 1.4 1.1 1.4 2.0 1.1 – 1.1 2.0
Intensity Change (Log2) – – – 0.14 0.14 -0.2 -0.17 – -0.35 -0.33 -0.58 -0.44 0.14 0.14 0.09 0.26 0.3 -0.05 – 0.03 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.6737 -1.5133 -1.4459 -1.8773 -1.1112 -1.5234 -0.9404 -0.6439 -1.0332 -1.1217 -0.4848 -0.6729 -2.2257 -2.0215 -1.9645 -1.7589 -1.5072 -1.9284
Actin 0.0083 0.0007 0.0158 0.0049 0.0012 0.0006 0.0264 0.0004 0.0006 0.0231 0.0252 0.001 0.0124 0.0016 0.0085 0.0158 0.0134 0.0032
Bud 0.0013 0 0.0013 0.0014 0 0 0.0008 0.0015 0.0002 0.0001 0.0008 0.0002 0.0009 0.0002 0.0007 0.0005 0.0013 0.0005
Bud Neck 0.0007 0.0001 0.0009 0.0001 0.0009 0.0018 0.0009 0 0.0002 0.0006 0.0003 0.0233 0.0014 0.0001 0.0007 0.0007 0.0018 0.0007
Bud Periphery 0.0016 0 0.0043 0.0009 0 0 0.0016 0.004 0.0007 0.0001 0.0011 0.0004 0.0013 0.0004 0.0015 0.0006 0.003 0.0031
Bud Site 0.0033 0.0003 0.0057 0.0011 0.0001 0 0.0067 0.0006 0.0007 0.0006 0.0018 0.0116 0.0041 0.0009 0.0044 0.0024 0.0033 0.0002
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0012 0.0004 0.0001 0.0001 0.0003 0.0004 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.0203 0.0268 0.0142 0.0253 0.0393 0.0528 0.0172 0.0313 0.0314 0.033 0.0092 0.0281 0.0264 0.0294 0.0304 0.0554 0.0215 0.0887
Cytoplasmic Foci 0.0314 0.0051 0.0191 0.0084 0.0408 0.0133 0.0492 0.0128 0.0093 0.0118 0.0165 0.0293 0.0301 0.0073 0.0139 0.017 0.0195 0.0154
Eisosomes 0.0009 0.0008 0.0004 0.0001 0.0001 0.0001 0.0008 0.0002 0.0002 0.0002 0.0004 0.0002 0.0004 0.0002 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0026 0.0003 0.0012 0.0019 0.004 0.0002 0.0058 0.0012 0.0003 0.0005 0.0008 0.0002 0.0027 0.0019 0.0022 0.001 0.0005 0.002
Endosome 0.0162 0.0013 0.0123 0.0249 0.0093 0.0041 0.019 0.0016 0.0006 0.0021 0.0344 0.0002 0.0292 0.0056 0.0231 0.0079 0.0165 0.0162
Golgi 0.004 0.0049 0.0118 0.0129 0.0023 0.0059 0.0096 0.0004 0.0004 0.0051 0.0198 0.0001 0.0044 0.0045 0.0032 0.0123 0.0209 0.0102
Lipid Particles 0.0263 0.0014 0.0126 0.0001 0.0327 0.0008 0.0127 0.0001 0.003 0.0036 0.0187 0.0002 0.0093 0.0008 0.0046 0.0089 0.0204 0.0018
Mitochondria 0.0069 0.0069 0.0054 0.0284 0.0004 0.0053 0.0165 0.003 0.0017 0.0008 0.0278 0.0001 0.0245 0.0081 0.0035 0.0588 0.1338 0.0093
None 0.8447 0.9498 0.8805 0.886 0.8277 0.9127 0.7914 0.938 0.9387 0.9035 0.7881 0.9034 0.8329 0.9363 0.8833 0.7738 0.711 0.838
Nuclear Periphery 0.0018 0.0001 0.0019 0.0007 0.0054 0.0001 0.0056 0.0007 0.0002 0.0002 0.001 0.0001 0.0038 0.0004 0.0018 0.0004 0.0004 0.0044
Nucleolus 0.0019 0.0002 0.0007 0 0.0009 0.0001 0.0027 0.0001 0.0004 0.0001 0.001 0.0001 0.001 0.0001 0.0022 0.0001 0.0004 0.0002
Nucleus 0.0022 0.0001 0.0008 0.0006 0.0058 0.0003 0.0044 0.0003 0.0002 0.0002 0.0006 0.0002 0.0011 0.0002 0.0014 0.0004 0.0003 0.0009
Peroxisomes 0.014 0.0005 0.0083 0.001 0.0209 0.0007 0.0065 0.0005 0.0058 0.0136 0.0376 0.0002 0.0074 0.0009 0.0046 0.041 0.0283 0.0003
Punctate Nuclear 0.0086 0.0003 0.0014 0.0002 0.0071 0.0006 0.0045 0.0004 0.0051 0.0003 0.0023 0.0006 0.0027 0.0004 0.0024 0.0004 0.0005 0.0007
Vacuole 0.0023 0.0002 0.0009 0.0005 0.0007 0.0002 0.0134 0.0018 0.0002 0.0004 0.0094 0.0002 0.0031 0.0007 0.0057 0.0017 0.0016 0.0026
Vacuole Periphery 0.0004 0.0001 0.0004 0.0007 0.0002 0.0001 0.0029 0.0007 0.0001 0.0001 0.0028 0 0.0007 0.0002 0.0016 0.0008 0.0013 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.8359 0.9963 3.6966 1.3768 1.8063 2.7848 0.8815 0.8499 0.7986 1.4221
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2035 1154 1843 1040 1695 1744 379 1140 3730 2898 2222 2180

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 601.91 605.50 829.82 771.62 669.03 678.13 836.22 793.74 632.41 649.21 830.91 783.19
Standard Deviation 59.03 106.75 87.79 111.02 73.89 92.35 111.43 107.87 74.16 104.57 92.28 109.94
Intensity Change Log 2 — 0.008579 0.463251 0.358343 — 0.019491 0.321812 0.246596 — 0.014333 0.390527 0.300599

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000374 0.000191 0.001054 0.000107 0.000183 0.000229 0.001344 0.000093 0.000259 0.000198 0.001206
Bud Neck 0.018628 0.019496 0.006403 0.013951 0.015822 0.044833 0.001371 0.009264 0.017353 0.034744 0.005544 0.011500
Bud Site 0.005033 0.008483 0.012720 0.061971 0.004017 0.020725 0.002400 0.047966 0.004571 0.015850 0.010960 0.054648
Cell Periphery 0.000116 0.000162 0.000113 0.000243 0.000105 0.000221 0.000180 0.000182 0.000111 0.000197 0.000124 0.000211
Cytoplasm 0.552168* 0.466784* 0.264208* 0.424256* 0.509787* 0.322096* 0.446823* 0.420643* 0.532909* 0.379711* 0.295356* 0.422367*
Cytoplasmic Foci 0.160532 0.190975* 0.004687 0.021367 0.222970* 0.179207 0.012004 0.019129 0.188905* 0.183893* 0.005935 0.020196
Eisosomes 0.000152 0.000119 0.000023 0.000053 0.000139 0.000148 0.000050 0.000046 0.000146 0.000136 0.000027 0.000049
Endoplasmic Reticulum 0.000290 0.000820 0.002570 0.002286 0.000437 0.001637 0.002788 0.001865 0.000357 0.001312 0.002607 0.002066
Endosome 0.006871 0.021994 0.001222 0.006170 0.010820 0.048572 0.002447 0.007578 0.008665 0.037989 0.001431 0.006906
Golgi 0.000944 0.002521 0.000047 0.005799 0.001795 0.005101 0.000079 0.007638 0.001331 0.004074 0.000052 0.006761
Lipid Particles 0.005230 0.008994 0.000765 0.001215 0.004745 0.010746 0.001472 0.001141 0.005009 0.010048 0.000886 0.001177
Mitochondria 0.003036 0.005398 0.000871 0.003105 0.002031 0.014099 0.000306 0.004402 0.002579 0.010634 0.000775 0.003783
Mitotic Spindle 0.000341 0.003922 0.002064 0.039586 0.001487 0.013305 0.001139 0.027636 0.000862 0.009568 0.001906 0.033337
None 0.005448 0.016438 0.004140 0.006893 0.002446 0.005520 0.006652 0.004774 0.004084 0.009867 0.004569 0.005785
Nuclear Periphery 0.000228 0.000821 0.002153 0.001190 0.000307 0.000969 0.001341 0.002021 0.000264 0.000910 0.002014 0.001625
Nuclear Periphery Foci 0.000256 0.000760 0.000379 0.000854 0.000281 0.000393 0.001824 0.000838 0.000267 0.000539 0.000626 0.000846
Nucleolus 0.001161 0.004456 0.000404 0.000505 0.000958 0.003567 0.000389 0.000474 0.001068 0.003921 0.000401 0.000489
Nucleus 0.180650* 0.175740* 0.672282* 0.299945* 0.166642* 0.140229* 0.445844* 0.329463* 0.174285* 0.154370* 0.633659* 0.315381*
Peroxisomes 0.002823 0.008491 0.000161 0.003102 0.004568 0.008865 0.000305 0.002493 0.003616 0.008716 0.000185 0.002783
Vacuole 0.055472 0.061346 0.024383 0.102605 0.049874 0.172877 0.072239 0.105758 0.052928 0.128465 0.032545 0.104254
Vacuole Periphery 0.000541 0.001907 0.000212 0.003849 0.000662 0.006707 0.000120 0.005343 0.000596 0.004795 0.000197 0.004630

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.77 -5.47 -4.76 -1.86 -3.31 -2.78 -6.27 -2.72 -2.39 -1.70 -3.62 -6.67 -4.62 -3.20 -3.22
Bud Neck -0.52 7.90 4.67 4.77 -3.17 -11.00 9.68 5.25 15.33 -5.24 -9.79 10.13 7.18 15.50 -3.30
Bud Site -2.53 -3.36 -7.75 -6.75 -5.59 -8.26 0.77 -7.09 -1.56 -7.28 -8.33 -3.62 -10.44 -5.31 -7.15
Cell Periphery -2.93 -0.47 -6.19 -1.89 -5.48 -8.59 -3.68 -4.00 4.81 1.40 -8.50 -2.47 -7.19 2.23 -4.09
Cytoplasm 9.11 40.39 22.75 11.33 -8.66 20.90 11.25 16.69 -1.68 -0.35 23.26 41.49 28.14 5.68 -9.95
Cytoplasmic Foci -4.54 41.47 37.52 29.94 -8.69 6.52 40.90 39.92 35.96 -3.10 0.99 58.25 54.06 46.85 -10.65
Eisosomes 2.78 18.05 11.38 5.09 -14.49 -0.63 4.79 5.93 11.99 1.91 1.06 15.81 11.34 12.19 -13.33
Endoplasmic Reticulum -5.00 -18.29 -18.01 -13.31 -0.25 -7.23 -12.81 -17.74 -6.49 5.13 -8.87 -21.37 -25.21 -13.07 2.73
Endosome -9.05 12.73 5.69 11.12 -4.08 -17.05 8.71 6.11 20.87 -1.90 -19.87 14.86 8.01 23.49 -5.07
Golgi -3.82 9.28 -1.13 0.36 -2.04 -4.41 6.70 -0.94 1.57 -2.40 -5.80 10.04 -1.51 1.69 -3.16
Lipid Particles -4.31 8.26 7.11 10.56 -5.60 -8.93 9.03 10.83 15.06 1.74 -9.08 12.39 11.14 18.38 -4.43
Mitochondria -2.36 3.35 1.91 4.40 -1.89 -8.52 7.37 -0.15 7.77 -2.73 -8.36 4.79 1.28 8.92 -2.69
Mitotic Spindle -3.99 -4.03 -6.91 -5.61 -6.23 -5.57 -0.62 -6.43 -1.84 -6.27 -6.60 -2.96 -9.49 -4.61 -8.74
None -4.43 2.12 0.36 4.60 -1.71 -2.88 -4.15 -2.95 1.38 1.69 -4.76 0.14 -0.73 4.39 -0.99
Nuclear Periphery -5.31 -28.85 -18.22 -9.64 2.02 -7.80 -15.38 -12.08 -8.09 -1.98 -9.58 -31.74 -19.41 -12.14 0.41
Nuclear Periphery Foci -4.76 -5.70 -11.45 -2.48 -7.00 -1.78 -7.72 -6.28 -5.05 4.22 -4.84 -9.51 -11.01 -5.97 -2.66
Nucleolus -7.29 6.51 6.91 9.01 0.70 -6.71 4.78 4.65 8.21 -0.33 -9.71 7.87 8.19 12.06 0.38
Nucleus 0.25 -68.54 -18.64 -16.56 30.79 4.51 -19.04 -21.22 -23.54 5.66 4.12 -70.51 -28.12 -28.99 33.28
Peroxisomes -6.71 6.53 2.39 7.95 -2.04 -5.58 8.85 4.89 9.87 -1.57 -9.17 10.83 5.10 12.76 -2.53
Vacuole -2.23 6.41 -19.50 -17.82 -21.70 -22.84 -9.62 -19.69 -3.28 -2.60 -20.75 -1.90 -27.61 -12.25 -24.22
Vacuole Periphery -6.37 4.68 -1.64 0.60 -2.22 -10.05 8.14 -1.99 4.81 -2.73 -11.19 7.68 -2.59 4.90 -3.44
Standard name
Human Ortholog
Description Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (35%), vacuole (2%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mip6

Mip6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mip6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available