Standard name
Description Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.96 0.98 1.0 0.97 0.91 0.87 0.9 0.86 0.81 0.84 1.0 1.0 0.99 0.98 0.96 0.98 0.92 0.93 0.93 0.93 0.92 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.08 0.08 0.09 0.06 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 0 2 4 3 0 0 0 0 0 0 1 0 0 0 1 2
Bud 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 1 0 0 1 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 3 1 2 3 4 7 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 239 138 27 52 131 146 152 181 107 147 95 138 341 462 567 122 143 113 222 123 56 205 295 292
Endoplasmic Reticulum 1 0 1 0 0 2 9 16 9 15 7 7 2 0 2 8 9 3 1 0 0 0 1 15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 3
Golgi 1 0 0 0 0 0 0 1 0 1 4 2 0 0 0 2 2 0 0 0 0 0 0 6
Mitochondria 0 0 0 1 0 3 1 1 3 5 7 6 0 0 0 0 1 0 0 0 0 0 0 0
Nucleus 2 0 0 0 0 1 0 1 1 0 0 2 0 0 0 0 0 0 3 2 0 3 3 16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 4 0 0 0 0 0 5 7 0 5 5 3 0 2 2 2 1 1 1 3 0 2 5 17
Unique Cell Count 247 138 28 53 131 151 167 208 119 171 117 165 342 463 573 125 149 115 242 133 60 220 320 373
Labelled Cell Count 247 138 28 53 132 152 173 213 122 178 126 170 343 464 573 135 158 120 242 133 60 220 320 373


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 97.3 100.8 75.5 62.2 68.5 55.5 50.5 39.8 41.4 40.6 34.1 34.1 96.1 91.2 87.8 108.7 127.6 152.4 90.1 86.1 91.8
Std Deviation (1e-4) 28.6 28.0 14.2 18.4 18.5 17.8 16.0 15.8 14.0 13.5 13.9 11.8 21.5 22.2 18.5 29.1 37.4 24.4 19.8 22.4 24.4
Intensity Change (Log2) -0.28 -0.14 -0.44 -0.58 -0.93 -0.87 -0.9 -1.15 -1.15 0.35 0.27 0.22 0.52 0.76 1.01 0.25 0.19 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 2.2 0.1 -1.0 -1.4 -1.1 -1.5 -2.0 -1.8 2.3 2.7 1.2 0.4 -0.1 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0