Standard name
Description Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.07 0.07 0 0 0 0 0 0 0.1 0.1 0.17 0.09 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.07 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.81 0.79 0.67 0.57 0.56 0.65 0.6 0.61 0.64 0.71 0.68 0.78 0.77 0.64 0.78 0.59 0.67 0.87 0.85 0.72 0.78 0.7 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0 0.05 0 0 0.09 0.14 0.18 0.13 0.13 0.16 0 0 0 0 0.07 0 0.07 0 0.15 0.06 0.11 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.15 0.12 0.24 0.38 0.36 0.23 0.25 0.17 0.18 0.12 0.15 0.14 0.18 0.2 0.15 0.26 0.2 0 0.06 0.07 0.1 0.12 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 1 0 1 0
Bud 0 0 0 0 0 1 0 0 1 2 0 1 0 0 0 0 0 0 5 1 2 1 7 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 2 1 3 3 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 6 12 20 21 14 16 15 12 9 4 13 13 30 7 8 7 1 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 3 4 0 0 0 0 0 0 0
Endosome 0 0 1 0 1 0 1 3 0 0 0 0 0 0 2 0 1 0 0 0 1 1 1 5
Golgi 0 0 1 2 6 2 2 3 1 0 0 0 0 0 2 0 0 0 0 0 2 3 0 2
Mitochondria 0 3 2 0 2 2 4 13 22 25 30 18 0 0 0 0 1 1 0 2 2 0 2 2
Nucleus 371 94 165 133 163 178 252 263 226 239 217 247 101 101 113 61 52 43 402 129 187 152 202 233
Nuclear Periphery 0 0 1 0 0 0 2 0 2 4 0 0 0 0 0 0 1 0 1 0 1 0 0 1
Nucleolus 5 6 9 9 11 14 35 60 66 49 40 58 1 1 3 1 6 1 30 6 39 12 32 32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 1 3
Vac/Vac Membrane 42 17 26 48 109 114 89 111 61 68 38 54 18 24 35 12 23 13 15 8 17 19 35 81
Unique Cell Count 411 116 208 199 287 319 385 440 369 373 307 363 130 132 177 78 88 64 462 152 261 195 290 379
Labelled Cell Count 421 124 211 204 312 333 399 471 395 402 337 385 134 139 186 84 98 66 462 152 261 195 290 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.2 15.3 19.5 21.8 22.2 21.4 20.0 18.4 17.6 15.9 16.0 16.5 19.4 19.4 19.5 14.2 16.3 18.9 21.0 21.7 23.3
Std Deviation (1e-4) 3.8 4.3 4.5 4.6 5.1 4.5 4.4 4.6 4.6 4.0 4.5 4.4 4.9 4.9 6.1 5.8 6.2 6.2 4.8 5.4 6.8
Intensity Change (Log2) 0.16 0.19 0.13 0.03 -0.08 -0.15 -0.3 -0.28 -0.24 -0.01 -0.01 -0.0 -0.46 -0.26 -0.05 0.11 0.16 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 2.0 1.9 0.5 0.5 0.7 0.2 0 0 2.8 2.7 4.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 3.2 4.1 2.5 0 0 0 0 0 0
Nucleus -2.8 -5.2 -5.5 -3.5 -4.9 -4.5 -3.8 -2.2 -2.9 -0.4 -0.6 -3.4 -0.2 -3.6 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 -0.3 0 2.1 3.6 4.7 3.4 3.3 4.2 0 0 -1.5 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 6.3 5.9 3.1 3.7 1.3 1.8 0 0.8 0.4 1.4 1.9 0.6 2.9 1.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available