Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0.05 0.06 0 0 0.07 0.06 0.09 0.05 0.09 0.07 0.05 0.05 0.09 0.06 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.06 0 0 0 0 0.06 0.07 0.07 0.06 0.08 0.07 0.18 0.05 0.16 0 0 0 0 0 0
Cytoplasm 0.05 0.11 0.05 0.1 0.1 0.06 0.09 0.05 0.09 0.06 0.1 0.1 0.07 0.13 0.1 0.36 0.42 0.38 0 0 0 0 0 0
Endoplasmic Reticulum 0.89 0.81 0.9 0.74 0.63 0.8 0.55 0.7 0.71 0.63 0.6 0.46 0.87 0.83 0.84 0.05 0.08 0 0.94 0.85 0.91 0.9 0.87 0.85
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.12 0.09 0.08 0.13 0.16 0.15 0.24 0.27 0.31 0 0 0 0.21 0.11 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.11 0.06 0.13 0.1 0.06 0.1 0.06 0.1 0.1 0.1 0.09 0.09 0.05 0 0 0.08 0 0.13 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.1 0.05 0.11 0.21 0.1 0.3 0.14 0.09 0.11 0.08 0.18 0.07 0 0 0.19 0.26 0.13 0 0.05 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 12 10 11 23 12 26 56 27 52 21 47 16 18 14 8 10 5 1 4 4 3 11 11
Bud 0 0 1 0 3 0 3 8 4 4 5 11 2 1 0 0 2 0 0 1 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 8 12 2 26 18 20 30 9 37 25 37 14 32 21 15 8 13 0 0 1 2 2 7
Cytoplasm 7 26 18 24 40 31 54 42 42 37 38 52 15 51 27 31 66 31 1 7 2 4 13 4
Endoplasmic Reticulum 125 190 313 177 258 388 334 586 336 358 231 245 198 317 238 4 13 0 143 221 336 265 445 417
Endosome 0 0 1 0 3 2 6 5 0 1 0 2 0 0 0 2 3 0 0 1 1 0 1 2
Golgi 6 0 10 22 37 9 10 19 2 7 1 4 5 5 1 2 12 8 1 1 2 2 4 4
Mitochondria 4 10 18 29 37 37 81 132 73 139 103 164 1 0 0 18 17 10 2 4 4 3 9 9
Nucleus 2 1 1 1 0 1 1 3 1 0 2 0 0 1 1 1 3 1 0 0 0 0 0 0
Nuclear Periphery 15 15 45 24 23 50 38 86 48 57 34 48 11 11 6 7 6 11 1 3 7 5 2 3
Nucleolus 0 0 0 0 0 1 0 4 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 18 23 18 25 85 49 181 117 44 61 30 98 15 16 11 16 42 11 0 12 2 4 14 25
Unique Cell Count 140 236 346 238 408 486 610 837 475 572 382 533 227 384 284 85 159 82 153 260 368 294 512 494
Labelled Cell Count 179 285 447 315 536 598 754 1088 587 756 490 711 277 452 319 104 182 90 153 260 368 294 512 494


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.3 11.5 11.3 11.7 12.6 11.8 11.9 12.0 11.9 11.3 11.4 11.1 13.9 14.4 14.1 8.0 9.6 11.9 15.4 15.0 15.5
Std Deviation (1e-4) 2.6 3.0 2.2 2.4 2.7 2.2 2.2 2.3 2.3 2.2 2.4 2.4 2.5 2.6 2.5 1.5 2.2 2.4 2.4 2.6 2.6
Intensity Change (Log2) 0.04 0.15 0.06 0.08 0.08 0.07 -0.01 0.01 -0.02 0.29 0.35 0.31 -0.51 -0.24 0.07 0.44 0.41 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.1 1.8 -0.4 1.1 2.6 1.9 3.6 1.7 3.5 2.3 1.3 1.3 0 1.8 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.0 1.8 0.2 -0.2 0.1 -1.4 2.0 1.9 2.2 1.5 2.8 2.2 4.8 0.8 0
Cytoplasm 2.2 2.4 0.7 2.1 -0.1 2.0 0.8 2.4 2.4 0.7 3.7 2.1 8.1 10.2 8.3
Endoplasmic Reticulum -5.2 -8.7 -4.1 -11.3 -7.5 -6.9 -9.2 -9.3 -13.4 -1.2 -3.1 -2.5 -16.1 -18.0 -16.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.3 3.5 -1.0 -1.3 -0.6 -2.9 -1.8 -2.9 -2.5 -0.5 -1.5 0 0 2.4 0
Mitochondria 3.0 2.0 1.4 3.9 5.0 4.6 7.4 7.9 9.1 -3.1 -4.5 -3.9 4.8 2.3 2.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.1 -3.5 -1.2 -3.6 -1.4 -1.3 -1.4 -1.8 -1.9 -3.2 -5.1 -5.0 -1.2 -3.2 0.1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 6.2 2.5 9.0 4.3 2.2 2.9 1.4 5.6 0.7 -0.7 -0.8 4.2 6.8 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9991 6.0085 6.5933 5.1328 6.2661 6.3397 5.2462 6.3767 5.376 5.2017 4.5525 5.4928 5.268 5.9007 4.9887 4.5925 4.1866 5.154
Actin 0.0083 0.0057 0.0043 0.003 0.0056 0.0043 0.0128 0.0001 0.0053 0.001 0.0079 0.0013 0.0005 0.0257 0.0004 0.0095 0.0005 0.0018
Bud 0.0002 0.0014 0.0029 0.0002 0.0099 0.0002 0.0003 0 0.0005 0.0001 0.0001 0.0001 0 0.0004 0 0.0001 0 0.0001
Bud Neck 0.0003 0.0004 0.0008 0.0004 0.0004 0.0008 0.0013 0.0001 0.0037 0.0003 0.0005 0.0006 0.0001 0.0006 0.0001 0.0003 0.0004 0.0012
Bud Periphery 0.0007 0.0033 0.0049 0.0002 0.0084 0.0004 0.0003 0 0.0023 0.0005 0.0003 0.0002 0 0.0009 0 0.0004 0.0001 0.0004
Bud Site 0.0009 0.01 0.0054 0.0002 0.0006 0.0001 0.0025 0.0001 0.001 0.0003 0.0001 0.0001 0.0001 0.0107 0.0001 0.0001 0 0.0003
Cell Periphery 0.0007 0.0021 0.0013 0.0003 0.0005 0.0004 0.0007 0.0009 0.0029 0.0015 0.0009 0.0009 0.0007 0.0028 0.0005 0.0006 0.0004 0.001
Cytoplasm 0.0446 0.1155 0.0622 0.054 0.026 0.0635 0.0433 0.0787 0.039 0.0737 0.0541 0.0651 0.0187 0.1098 0.0443 0.0564 0.0305 0.0827
Cytoplasmic Foci 0.0077 0.007 0.0059 0.0101 0.0167 0.0035 0.004 0.0014 0.0025 0.0013 0.0026 0.0032 0.0125 0.0068 0.0028 0.0063 0.0012 0.0024
Eisosomes 0.0004 0.0002 0.0002 0 0.0001 0.0001 0.0002 0 0.0005 0.0001 0.0003 0 0 0.0001 0 0.0002 0 0
Endoplasmic Reticulum 0.7993 0.6742 0.7967 0.7883 0.8011 0.7988 0.8147 0.8447 0.8412 0.8359 0.8451 0.8133 0.8574 0.7811 0.8718 0.8479 0.8865 0.8397
Endosome 0.0187 0.0248 0.021 0.0275 0.0222 0.0157 0.0176 0.0022 0.0095 0.0054 0.0124 0.0173 0.014 0.0104 0.0081 0.0226 0.0058 0.0053
Golgi 0.0032 0.003 0.0035 0.0048 0.0035 0.0023 0.0055 0.0003 0.0027 0.0009 0.0046 0.0012 0.0013 0.004 0.0012 0.004 0.0004 0.0004
Lipid Particles 0.0085 0.0156 0.0086 0.035 0.0083 0.0027 0.0039 0.0016 0.0048 0.0011 0.0066 0.0046 0.0243 0.0075 0.0047 0.0117 0.0012 0.0034
Mitochondria 0.0023 0.0027 0.0019 0.0008 0.0068 0.0017 0.0007 0 0.0105 0.0507 0.0006 0.0004 0.0003 0.0008 0.0003 0.0005 0.0001 0.0002
None 0.0249 0.0475 0.024 0.0059 0.0074 0.0295 0.0426 0.0618 0.0453 0.0037 0.041 0.046 0.0204 0.0011 0.0496 0.022 0.0501 0.037
Nuclear Periphery 0.0593 0.0566 0.0313 0.0513 0.0279 0.0572 0.0377 0.0052 0.0207 0.0146 0.0172 0.0355 0.041 0.0237 0.0121 0.0125 0.0167 0.0174
Nucleolus 0.0002 0.0006 0.0004 0.0002 0.0006 0.0002 0.0003 0 0.0001 0.0001 0 0 0.0001 0.0003 0 0 0 0
Nucleus 0.0065 0.0099 0.0077 0.0067 0.005 0.0102 0.0047 0.0004 0.0018 0.0021 0.0018 0.0034 0.0014 0.0043 0.0008 0.0008 0.0013 0.0025
Peroxisomes 0.0021 0.0003 0.0027 0.0008 0.0142 0.0004 0.0006 0 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0 0
Punctate Nuclear 0.0027 0.0023 0.0071 0.0042 0.0024 0.0014 0.0015 0.0002 0.0006 0.0002 0.0006 0.0006 0.0009 0.0009 0.0005 0.0007 0.0004 0.0003
Vacuole 0.0066 0.0147 0.0057 0.0047 0.0263 0.0048 0.0038 0.002 0.0041 0.0053 0.0025 0.0052 0.0042 0.0069 0.0021 0.0026 0.0036 0.0033
Vacuole Periphery 0.0019 0.0024 0.0015 0.0015 0.0061 0.0018 0.0011 0.0002 0.0009 0.0011 0.0006 0.0011 0.0019 0.0009 0.0005 0.0008 0.0008 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.0235 46.3877 65.439 95.4682 86.1325 43.3139 51.6701 55.3112 74.4498 98.5287
Translational Efficiency 0.725 0.8168 0.7347 0.5797 0.7199 0.8332 0.7573 0.5752 0.6512 0.5989

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
109 1008 423 1445 1317 1405 1581 1479 1426 2413 2004 2924

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1291.57 1160.44 1393.80 1327.63 1066.72 1063.83 1443.03 1359.93 1083.91 1104.19 1432.64 1343.97
Standard Deviation 185.46 243.40 369.22 347.70 237.23 219.29 279.01 316.86 241.19 234.56 300.98 332.85
Intensity Change Log 2 -0.154454 0.109898 0.039727 -0.003914 0.435920 0.350351 -0.084409 0.266539 0.188556

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003099 0.000992 0.002417 0.003464 0.001930 0.002286 0.002140 0.003176 0.002019 0.001745 0.002198 0.003318
Bud Neck 0.006882 0.000410 0.004137 0.005670 0.000276 0.000774 0.001398 0.005020 0.000781 0.000622 0.001976 0.005341
Bud Site 0.023245 0.004320 0.008795 0.025538 0.007381 0.005037 0.007155 0.023676 0.008593 0.004737 0.007502 0.024596
Cell Periphery 0.002856 0.001428 0.002144 0.005235 0.001205 0.002266 0.002471 0.004756 0.001331 0.001916 0.002402 0.004993
Cytoplasm 0.018744 0.022415 0.020462 0.070099 0.011037 0.025739 0.021316 0.044143 0.011626 0.024350 0.021136 0.056970
Cytoplasmic Foci 0.026500 0.008163 0.005232 0.019344 0.011281 0.007673 0.004079 0.006210 0.012444 0.007878 0.004322 0.012701
Eisosomes 0.000332 0.000047 0.000046 0.000203 0.000059 0.000183 0.000104 0.000152 0.000080 0.000126 0.000092 0.000177
Endoplasmic Reticulum 0.650143* 0.909536* 0.806687* 0.676870* 0.903941* 0.911399* 0.886364* 0.810647* 0.884541* 0.910621* 0.869546* 0.744536*
Endosome 0.026199 0.011348 0.028380 0.021885 0.017050 0.007427 0.012237 0.015536 0.017749 0.009065 0.015644 0.018674
Golgi 0.024197 0.003877 0.012363 0.007282 0.007570 0.003645 0.003195 0.005427 0.008841 0.003742 0.005130 0.006344
Lipid Particles 0.022852 0.008735 0.006117 0.028919 0.006845 0.007801 0.010126 0.012453 0.008069 0.008191 0.009280 0.020590
Mitochondria 0.002911 0.001597 0.000944 0.002558 0.002784 0.002586 0.001651 0.002190 0.002794 0.002173 0.001502 0.002372
Mitotic Spindle 0.018020 0.000187 0.000362 0.011205 0.000195 0.000150 0.001137 0.004244 0.001557 0.000165 0.000974 0.007684
None 0.000041 0.004316 0.000882 0.004493 0.004425 0.005325 0.001636 0.001890 0.004089 0.004903 0.001477 0.003177
Nuclear Periphery 0.004386 0.001786 0.011960 0.005529 0.002313 0.001302 0.005555 0.002404 0.002471 0.001504 0.006907 0.003949
Nuclear Periphery Foci 0.064968 0.010992 0.059900 0.046750 0.012890 0.006952 0.017588 0.023598 0.016871 0.008640 0.026519 0.035039
Nucleolus 0.000086 0.000064 0.000234 0.001251 0.000058 0.000228 0.000046 0.000076 0.000060 0.000159 0.000086 0.000657
Nucleus 0.001283 0.002066 0.005345 0.018148 0.001433 0.003085 0.005246 0.008365 0.001422 0.002659 0.005267 0.013200
Peroxisomes 0.001461 0.000350 0.000125 0.000717 0.000576 0.001376 0.000164 0.000292 0.000644 0.000948 0.000156 0.000502
Vacuole 0.092823 0.006328 0.020178 0.042836 0.004783 0.003301 0.015337 0.023845 0.011513 0.004566 0.016359 0.033230
Vacuole Periphery 0.008971 0.001045 0.003290 0.002003 0.001970 0.001464 0.001054 0.001899 0.002505 0.001289 0.001526 0.001950

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.75 0.49 -0.55 -4.67 -1.70 -0.43 -0.45 -2.29 -1.61 -1.59 0.42 -0.45 -2.86 -3.63 -2.18
Bud Neck 3.24 0.94 0.37 -8.78 -0.98 -2.35 -6.22 -6.51 -5.73 -4.81 0.86 -3.72 -9.30 -9.85 -5.91
Bud Site 2.49 1.85 -0.56 -9.14 -6.16 1.36 -0.25 -7.29 -8.62 -7.24 2.45 0.31 -8.88 -12.55 -9.93
Cell Periphery 1.69 0.92 -1.72 -5.28 -3.96 -2.15 -2.94 -6.01 -3.37 -3.12 -1.65 -2.89 -7.76 -5.81 -4.52
Cytoplasm -0.08 0.12 -6.63 -10.61 -9.81 -4.92 -5.21 -10.99 -6.33 -7.07 -5.22 -5.00 -16.92 -12.11 -12.51
Cytoplasmic Foci 2.06 2.43 0.74 -4.97 -7.25 1.76 3.91 2.47 0.68 -2.29 2.35 4.53 -0.50 -3.98 -7.66
Eisosomes 2.45 2.44 1.03 -3.40 -3.31 -3.25 -1.68 -4.40 0.60 -1.54 -1.84 -0.58 -3.61 -1.67 -2.67
Endoplasmic Reticulum -7.00 -4.15 -0.25 21.15 9.19 -0.82 3.28 11.88 12.51 9.05 -3.40 2.60 18.07 23.98 16.48
Endosome 3.51 -0.47 1.02 -6.87 2.32 6.83 2.93 0.90 -6.61 -2.20 6.64 1.19 -0.67 -9.90 -2.27
Golgi 2.06 1.11 1.73 -2.37 1.90 3.60 3.82 1.74 -1.51 -1.84 4.12 2.65 2.02 -2.83 -0.90
Lipid Particles 3.02 3.62 -1.10 -7.78 -9.33 -0.68 -2.35 -3.57 -3.00 -0.97 -0.08 -1.16 -7.76 -8.28 -6.48
Mitochondria 0.66 0.97 0.12 -2.21 -3.34 0.21 1.10 0.33 0.14 -1.20 0.77 1.42 0.28 -1.01 -2.03
Mitotic Spindle 1.72 1.70 0.54 -6.69 -6.58 0.54 -2.68 -5.19 -5.24 -3.90 1.73 0.58 -5.58 -8.40 -7.23
None -3.31 -3.15 -3.92 -0.04 -3.07 -0.52 1.96 1.88 2.52 -0.41 -0.56 2.05 0.72 1.62 -2.12
Nuclear Periphery 1.58 -3.40 -0.70 -4.89 3.71 1.32 -3.09 -0.21 -2.17 3.42 1.41 -4.64 -2.10 -5.31 3.57
Nuclear Periphery Foci 3.97 0.30 1.28 -10.07 1.85 3.04 -2.18 -3.75 -6.61 -1.94 3.98 -3.76 -6.66 -12.20 -2.96
Nucleolus 0.71 -1.30 -2.75 -2.89 -2.32 -2.59 0.33 -1.38 2.18 -1.68 -2.43 -1.22 -2.95 -2.38 -2.77
Nucleus -0.44 -2.19 -10.78 -10.78 -6.86 -1.95 -3.91 -8.16 -7.03 -3.66 -1.73 -4.31 -12.80 -12.69 -7.84
Peroxisomes 1.41 1.71 0.95 -3.06 -5.99 -2.98 3.44 2.32 4.23 -1.50 -1.65 3.95 1.06 2.80 -4.95
Vacuole 4.76 4.05 2.36 -13.46 -7.88 1.69 -6.66 -10.51 -11.11 -4.97 4.10 -2.54 -11.29 -17.58 -9.60
Vacuole Periphery 1.40 0.97 1.24 -1.78 1.71 1.05 2.35 0.26 -0.64 -1.50 2.17 1.69 1.09 -1.49 -0.83