Standard name
Human Ortholog
Description RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.96 0.93 0.88 0.86 0.74 0.77 0.67 0.69 0.66 0.66 0.96 0.95 0.93 0.87 0.85 0.88 0.94 0.94 0.94 0.91 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.1 0.06 0.25 0.23 0.27 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.1 0.13 0.11 0.19 0.18 0.14 0.12 0.1 0.08 0 0.09 0.11 0.21 0.21 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 2 4 3 5 0 0 0 0 0 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 4 3 2 0 0 2 4 0 0 0 2 1 1 0 0 0 0 0 0
Cytoplasm 128 225 133 149 167 166 166 136 88 136 166 190 85 221 265 198 182 143 131 232 141 158 191 229
Endoplasmic Reticulum 2 3 1 1 1 1 2 1 3 2 2 13 1 1 3 7 4 6 0 0 0 1 1 3
Endosome 0 0 0 0 0 2 0 0 0 2 2 3 0 0 0 0 1 1 1 1 1 1 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 3 0 0 0 10 22 10 33 45 68 73 1 0 0 7 5 0 0 3 2 1 1 0
Nucleus 0 0 3 2 4 4 11 10 4 8 13 11 1 1 3 5 6 6 0 2 2 3 9 9
Nuclear Periphery 0 1 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 4 3 16 25 21 43 31 19 23 25 23 1 21 32 47 44 28 1 4 1 3 1 4
Unique Cell Count 130 232 138 161 189 192 223 177 132 198 253 287 89 232 285 228 213 163 139 248 151 174 215 259
Labelled Cell Count 132 236 141 168 197 209 250 190 149 221 285 325 90 244 303 267 243 187 139 248 151 174 215 259


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 6.5 9.1 8.4 8.6 7.5 6.8 7.1 5.9 6.1 5.7 5.9 7.2 8.7 7.8 8.4 10.7 11.2 9.3 9.9 9.6
Std Deviation (1e-4) 1.3 1.0 1.0 1.2 1.4 1.8 1.7 1.7 1.0 1.4 1.0 1.1 1.3 1.4 1.1 1.6 2.0 2.2 1.4 1.4 1.5
Intensity Change (Log2) – – – -0.12 -0.08 -0.28 -0.41 -0.37 -0.63 -0.58 -0.67 -0.63 -0.34 -0.07 -0.22 -0.11 0.22 0.3 0.03 0.11 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -2.6 -3.0 -5.4 -4.9 -6.3 -6.2 -6.9 -6.8 -0.3 -0.5 -1.4 -3.0 -3.3 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 0 6.3 6.0 6.7 6.5 0 0 0 0 0 0
Nucleus 0 0 0 1.3 1.5 0 0 1.4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.7 3.5 3.0 4.7 4.4 3.7 3.2 2.8 2.4 0 2.6 3.2 5.0 5.0 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4831 5.7204 5.0765 5.3071 4.918 5.0834 7.0026 8.5138 7.458 7.0885 7.6553 8.355 4.8414 6.253 5.2723 5.8073 5.1104 5.7533
Actin 0.0006 0.0003 0.0008 0.0001 0.0002 0.0042 0.0375 0.001 0.0119 0.0008 0.0046 0.0048 0.0169 0.0006 0.0021 0.0003 0.0648 0.0068
Bud 0.0023 0.0001 0.0042 0.0001 0.0001 0.0001 0.001 0.0006 0.0006 0.0005 0.0004 0.0001 0.0005 0.0013 0.0001 0.0001 0.0002 0.0002
Bud Neck 0.0004 0.0002 0.0004 0.0004 0.0004 0.0013 0.0015 0.0002 0.0004 0.0004 0.0006 0.0014 0.0006 0.003 0.0004 0.0003 0.0004 0.0016
Bud Periphery 0.0006 0 0.0016 0 0 0 0.0014 0.0001 0.0003 0.0003 0.0001 0 0.0006 0.0014 0.0001 0 0.0001 0.0003
Bud Site 0.0006 0.0004 0.0005 0 0.0001 0.0001 0.0058 0.0012 0.0008 0.0007 0.0002 0.0001 0.0009 0.0021 0.0004 0 0.0003 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0012 0.0001 0.0001 0 0 0 0.0001 0.0003 0 0 0 0
Cytoplasm 0.9446 0.9704 0.9519 0.9849 0.9673 0.95 0.7485 0.9586 0.9138 0.872 0.96 0.9527 0.9153 0.9632 0.9492 0.9809 0.9132 0.9437
Cytoplasmic Foci 0.0049 0.0032 0.0056 0.0023 0.0027 0.0057 0.0396 0.0013 0.0174 0.0301 0.0078 0.0071 0.0064 0.0035 0.0056 0.0013 0.0035 0.0043
Eisosomes 0 0 0 0 0 0.0001 0.0009 0 0 0 0 0 0.0001 0.0002 0 0 0.0001 0
Endoplasmic Reticulum 0.009 0.0026 0.0029 0.001 0.0018 0.004 0.0059 0.0007 0.0022 0.0026 0.0018 0.0015 0.0048 0.0031 0.0021 0.0079 0.0025 0.0021
Endosome 0.0049 0.0022 0.0026 0.0009 0.0086 0.01 0.0337 0.0003 0.0087 0.0279 0.0031 0.0053 0.0136 0.0029 0.0054 0.0017 0.0018 0.0029
Golgi 0.0002 0.0001 0.0002 0 0.0002 0.0028 0.0167 0.0001 0.0031 0.0027 0.0013 0.0027 0.0049 0.001 0.0008 0 0.002 0.0027
Lipid Particles 0.0001 0 0.0001 0 0 0.0005 0.0092 0 0.002 0.0013 0 0.0023 0.0015 0.0017 0.0055 0 0.0005 0.0044
Mitochondria 0.0003 0.0002 0.0003 0.0001 0.0003 0.0007 0.0042 0.0001 0.0013 0.0009 0.0005 0.0022 0.0031 0.0006 0.0018 0.0001 0.0005 0.0024
None 0.0123 0.0048 0.0043 0.002 0.0017 0.0076 0.0528 0.0338 0.0224 0.0507 0.0151 0.0089 0.014 0.0088 0.0037 0.0021 0.0017 0.0073
Nuclear Periphery 0.0057 0.0022 0.0021 0.0011 0.0024 0.0021 0.0044 0.0003 0.0017 0.001 0.0005 0.0013 0.003 0.0007 0.002 0.002 0.0006 0.0013
Nucleolus 0 0 0 0 0 0 0.0003 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0024 0 0 0
Nucleus 0.0105 0.0106 0.0204 0.0062 0.0102 0.0086 0.0076 0.0015 0.0045 0.0032 0.0023 0.0037 0.0089 0.0029 0.0055 0.0024 0.005 0.0095
Peroxisomes 0.0002 0.0001 0.0002 0 0 0.0002 0.0158 0 0.0049 0.0011 0.0009 0.0034 0.0008 0.0005 0.0068 0 0.0019 0.0087
Punctate Nuclear 0.0008 0.0014 0.001 0.0002 0.0004 0.0006 0.0076 0.0001 0.0032 0.0013 0.0004 0.0017 0.0007 0.0002 0.005 0.0001 0.0002 0.0009
Vacuole 0.0016 0.0009 0.0008 0.0005 0.0029 0.0009 0.0031 0.0001 0.0005 0.0017 0.0002 0.0004 0.0023 0.0017 0.0009 0.0006 0.0004 0.0005
Vacuole Periphery 0.0003 0.0001 0.0002 0.0001 0.0007 0.0004 0.0012 0 0.0002 0.0005 0.0001 0.0002 0.0009 0.0002 0.0002 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3361 59.9014 31.6085 26.8229 16.4105 16.3708 33.9844 35.8819 24.6573 20.0292
Translational Efficiency 1.5916 1.1225 1.9092 1.6356 1.884 1.6404 1.5611 1.6307 2.1086 1.5351

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1235 568 1974 2324 1493 2216 1921 1775 2728 2784 3895 4099

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 807.63 996.21 1156.23 1299.19 888.83 1027.10 1180.04 1280.36 852.07 1020.80 1167.97 1291.04
Standard Deviation 94.48 142.83 135.56 172.98 115.55 130.46 157.80 186.43 113.94 133.66 147.44 179.17
Intensity Change Log 2 — 0.302755 0.517662 0.685846 — 0.208597 0.408856 0.526570 — 0.254190 0.461680 0.604592

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000166 0.000766 0.000383 0.001978 0.000632 0.000498 0.000845 0.002018 0.000421 0.000553 0.000611 0.001995
Bud Neck 0.011755 0.004475 0.003601 0.006863 0.002945 0.005221 0.003794 0.004923 0.006933 0.005069 0.003697 0.006023
Bud Site 0.003857 0.018708 0.007723 0.027564 0.009978 0.011527 0.015793 0.038926 0.007207 0.012992 0.011703 0.032484
Cell Periphery 0.000277 0.000179 0.000064 0.000113 0.000151 0.000085 0.000103 0.000166 0.000208 0.000104 0.000083 0.000136
Cytoplasm 0.427103* 0.634855* 0.758116* 0.658584* 0.750282* 0.699231* 0.707983* 0.741471* 0.603975* 0.686097* 0.733391* 0.694477*
Cytoplasmic Foci 0.138574 0.168281 0.014813 0.018603 0.100973 0.110798 0.019443 0.023088 0.117996 0.122526 0.017097 0.020545
Eisosomes 0.000146 0.000044 0.000008 0.000018 0.000029 0.000036 0.000015 0.000017 0.000082 0.000038 0.000011 0.000018
Endoplasmic Reticulum 0.004768 0.003040 0.000664 0.001048 0.002810 0.001426 0.001286 0.001113 0.003697 0.001755 0.000971 0.001076
Endosome 0.010226 0.014718 0.000761 0.004909 0.004951 0.006095 0.002049 0.001706 0.007339 0.007855 0.001396 0.003522
Golgi 0.001130 0.003490 0.000169 0.007503 0.001234 0.002702 0.000626 0.001558 0.001187 0.002863 0.000395 0.004929
Lipid Particles 0.011687 0.003431 0.000800 0.001283 0.001799 0.001677 0.000955 0.001240 0.006275 0.002035 0.000877 0.001264
Mitochondria 0.002398 0.000917 0.000300 0.003712 0.000850 0.002902 0.000325 0.000535 0.001551 0.002497 0.000313 0.002336
Mitotic Spindle 0.000959 0.002583 0.006232 0.056789 0.002257 0.004950 0.013221 0.012485 0.001669 0.004467 0.009679 0.037604
None 0.005920 0.001905 0.002957 0.003807 0.003880 0.002522 0.005069 0.004675 0.004803 0.002396 0.003999 0.004183
Nuclear Periphery 0.001664 0.000235 0.000395 0.001213 0.000189 0.000256 0.000514 0.000323 0.000857 0.000252 0.000453 0.000828
Nuclear Periphery Foci 0.001225 0.005773 0.001924 0.002290 0.003040 0.001079 0.003620 0.004225 0.002218 0.002037 0.002760 0.003128
Nucleolus 0.001812 0.000528 0.000157 0.000570 0.000367 0.000626 0.000283 0.000206 0.001021 0.000606 0.000219 0.000412
Nucleus 0.250073* 0.056776 0.157529* 0.126486 0.083174 0.086292 0.163185* 0.106137 0.158731* 0.080270 0.160318* 0.117674
Peroxisomes 0.004023 0.003002 0.000330 0.002686 0.001076 0.002406 0.000700 0.001156 0.002410 0.002528 0.000512 0.002023
Vacuole 0.120071 0.075483 0.042472 0.070063 0.028516 0.058675 0.059865 0.053366 0.069964 0.062104 0.051050 0.062833
Vacuole Periphery 0.002166 0.000810 0.000603 0.003916 0.000868 0.000995 0.000324 0.000666 0.001456 0.000957 0.000465 0.002509

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.00 -4.14 -7.06 -3.43 -5.44 1.04 -2.03 -5.18 -5.98 -3.98 -1.35 -2.99 -7.96 -7.43 -6.28
Bud Neck 3.94 4.80 3.16 -2.03 -4.19 -2.78 -1.96 -3.05 0.18 -1.34 2.01 3.35 1.22 -1.54 -4.31
Bud Site -7.01 -3.88 -10.45 -0.23 -7.47 -0.92 -2.33 -8.64 -8.18 -6.62 -5.19 -3.43 -12.34 -7.47 -9.38
Cell Periphery 4.01 10.71 8.37 4.45 -5.81 6.76 3.52 -0.95 -8.98 -5.09 9.57 11.04 6.75 -4.53 -7.06
Cytoplasm -14.83 -30.29 -14.81 5.17 18.85 7.29 16.80 14.32 7.49 -2.14 -10.68 -8.37 1.16 13.83 11.75
Cytoplasmic Foci -3.16 27.43 27.47 19.98 -0.16 -2.14 23.88 22.56 26.79 -2.43 -0.94 36.16 35.61 34.42 -1.68
Eisosomes 10.26 14.81 13.66 6.70 -9.49 -1.88 3.91 1.27 2.77 -3.35 8.14 14.95 13.17 4.99 -7.70
Endoplasmic Reticulum 2.43 7.87 7.80 5.22 -0.32 4.26 5.56 7.35 1.21 3.57 5.75 9.73 10.54 3.59 3.03
Endosome -2.44 15.99 12.82 7.49 -4.33 -1.83 7.39 8.86 10.20 1.54 -0.69 16.92 14.88 11.74 -2.24
Golgi -2.33 6.48 -1.16 1.34 -2.93 -3.42 6.65 2.19 4.21 -1.02 -4.16 9.63 -0.37 2.80 -3.06
Lipid Particles 6.97 11.23 9.95 1.47 -7.16 0.05 2.10 -0.94 -0.67 -4.45 8.05 11.17 9.06 -0.35 -8.49
Mitochondria 2.42 4.48 1.70 -0.71 -2.32 -3.00 1.70 1.45 4.07 -1.25 -1.60 4.60 1.72 2.79 -2.42
Mitotic Spindle -1.51 -5.32 -11.82 -10.93 -9.02 -1.96 -5.84 -6.58 -4.89 -0.73 -2.73 -7.72 -13.31 -11.02 -7.86
None 3.36 2.82 1.17 -5.51 -7.86 3.57 -1.60 -1.40 -5.95 0.17 4.34 2.10 0.45 -8.86 -4.31
Nuclear Periphery 6.61 4.55 1.36 -9.27 -5.48 -1.37 -18.38 -15.20 -6.44 5.82 5.59 0.62 -1.55 -9.88 -3.49
Nuclear Periphery Foci -6.92 -4.20 -4.85 5.00 -0.22 7.72 -2.69 -2.91 -8.58 -0.80 0.64 -4.27 -4.33 -4.95 -0.39
Nucleolus 4.00 6.16 3.92 -0.48 -3.60 -1.45 0.72 1.17 2.16 0.71 2.22 6.02 3.57 0.75 -3.22
Nucleus 25.81 10.46 12.58 -21.58 3.11 -0.98 -22.71 -12.62 -12.85 11.32 18.32 -5.39 2.14 -22.81 9.93
Peroxisomes 1.19 4.33 2.90 2.41 -3.76 -3.60 2.45 0.27 3.87 -2.42 -0.04 4.84 2.71 3.74 -4.26
Vacuole 5.73 6.35 -9.37 -15.12 -18.03 -13.22 -21.59 -23.64 -15.00 -2.54 1.09 -9.18 -22.99 -25.45 -14.66
Vacuole Periphery 3.57 4.19 2.39 -0.65 -1.70 -0.57 4.64 3.53 4.41 -0.77 2.51 5.43 2.94 1.16 -1.93
Standard name
Human Ortholog
Description RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

She2

She2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (She2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available