Standard name
Description RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.96 0.93 0.88 0.86 0.74 0.77 0.67 0.69 0.66 0.66 0.96 0.95 0.93 0.87 0.85 0.88 0.94 0.94 0.94 0.91 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.1 0.06 0.25 0.23 0.27 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.1 0.13 0.11 0.19 0.18 0.14 0.12 0.1 0.08 0 0.09 0.11 0.21 0.21 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 2 4 3 5 0 0 0 0 0 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 4 3 2 0 0 2 4 0 0 0 2 1 1 0 0 0 0 0 0
Cytoplasm 128 225 133 149 167 166 166 136 88 136 166 190 85 221 265 198 182 143 131 232 141 158 191 229
Endoplasmic Reticulum 2 3 1 1 1 1 2 1 3 2 2 13 1 1 3 7 4 6 0 0 0 1 1 3
Endosome 0 0 0 0 0 2 0 0 0 2 2 3 0 0 0 0 1 1 1 1 1 1 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 3 0 0 0 10 22 10 33 45 68 73 1 0 0 7 5 0 0 3 2 1 1 0
Nucleus 0 0 3 2 4 4 11 10 4 8 13 11 1 1 3 5 6 6 0 2 2 3 9 9
Nuclear Periphery 0 1 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 4 3 16 25 21 43 31 19 23 25 23 1 21 32 47 44 28 1 4 1 3 1 4
Unique Cell Count 130 232 138 161 189 192 223 177 132 198 253 287 89 232 285 228 213 163 139 248 151 174 215 259
Labelled Cell Count 132 236 141 168 197 209 250 190 149 221 285 325 90 244 303 267 243 187 139 248 151 174 215 259


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 6.5 9.1 8.4 8.6 7.5 6.8 7.1 5.9 6.1 5.7 5.9 7.2 8.7 7.8 8.4 10.7 11.2 9.3 9.9 9.6
Std Deviation (1e-4) 1.3 1.0 1.0 1.2 1.4 1.8 1.7 1.7 1.0 1.4 1.0 1.1 1.3 1.4 1.1 1.6 2.0 2.2 1.4 1.4 1.5
Intensity Change (Log2) -0.12 -0.08 -0.28 -0.41 -0.37 -0.63 -0.58 -0.67 -0.63 -0.34 -0.07 -0.22 -0.11 0.22 0.3 0.03 0.11 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -2.6 -3.0 -5.4 -4.9 -6.3 -6.2 -6.9 -6.8 -0.3 -0.5 -1.4 -3.0 -3.3 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 0 6.3 6.0 6.7 6.5 0 0 0 0 0 0
Nucleus 0 0 0 1.3 1.5 0 0 1.4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.7 3.5 3.0 4.7 4.4 3.7 3.2 2.8 2.4 0 2.6 3.2 5.0 5.0 4.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (She2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available