Standard name
Description Protein of unknown function; required for survival upon exposure to K1 killer toxin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.06
Bud 0 0 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0.05 0 0 0.19 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0.05 0 0.09 0.06 0.11 0.07 0.07 0.05 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.27 0.16 0.05 0.09 0.05 0.07 0.11 0.1
Mitochondria 0.95 0.92 0.95 0.92 0.88 0.88 0.89 0.96 0.94 0.94 0.97 0.84 0.87 0.84 0 0.1 0.77 0.68 0.76 0.73 0.62 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.05 0 0 0.07 0.07 0.09 0.06 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.09 0.07 0.11 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.09 0.13 0.12 0 0.06 0.06 0 0.05 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.19 0.07 0 0.07 0 0 0 0 0 0 0.13 0.09 0.13 0.1 0.1 0.05 0.05 0 0.06 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 2 0 1 0 0 0 0 0 0 0 2 0 5 6 3 1 0 2 9 21
Bud 5 1 5 4 7 2 8 5 17 8 5 5 5 11 5 9 0 0 0 0 1 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1
Bud Site 1 0 0 1 0 1 5 1 8 3 4 1 0 0 3 6
Cell Periphery 5 1 2 4 0 0 2 0 1 0 3 10 5 8 8 4 0 0 0 0 0 0
Cytoplasm 0 1 3 9 8 6 9 7 7 6 6 11 15 15 17 15 0 0 1 1 1 3
Endoplasmic Reticulum 6 1 1 0 1 1 1 1 1 1 3 1 5 1 1 8 0 0 0 0 5 0
Endosome 1 0 0 0 9 3 1 0 1 0 0 12 14 34 5 11 24 6 3 4 14 25
Golgi 5 3 0 0 3 1 0 0 0 0 0 6 7 16 24 17 18 9 3 8 30 34
Mitochondria 332 88 57 105 141 140 187 223 307 213 275 190 308 322 4 10 270 70 54 84 165 191
Nucleus 0 0 2 0 0 3 3 3 3 0 4 1 1 0 4 4 0 0 0 0 1 2
Nuclear Periphery 18 1 3 0 7 12 14 20 20 12 23 1 13 4 1 3 3 0 0 1 3 4
Nucleolus 7 3 0 4 7 15 15 25 23 16 13 3 5 2 0 3 0 0 0 0 2 3
Peroxisomes 0 2 0 1 6 5 5 1 7 1 1 19 25 34 12 12 7 6 4 2 14 16
SpindlePole 2 0 0 0 3 1 4 3 8 3 5 3 4 6 6 12 1 0 0 0 1 2
Vac/Vac Membrane 53 18 4 3 11 7 6 4 9 6 9 30 33 49 9 10 18 4 1 7 12 25
Unique Cell Count 349 96 60 114 160 160 209 232 325 227 284 226 354 384 89 104 351 104 71 117 268 347
Labelled Cell Count 437 120 79 131 204 197 260 293 412 269 351 293 442 502 105 130 351 104 71 117 268 347


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.7 4.3 5.2 6.0 5.3 5.1 4.4 4.3 4.6 4.6 6.7 6.7 6.6 7.2 7.6 6.5 7.3 7.4
Std Deviation (1e-4) 0.9 1.3 0.9 1.8 2.1 2.7 1.9 1.4 1.0 2.2 1.3 1.8 1.9 1.9 2.0 2.3 1.6 1.8 2.0
Intensity Change (Log2) 0.28 0.5 0.31 0.27 0.04 0.01 0.12 0.1 0.66 0.65 0.63 0.76 0.83 0.62 0.78 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 0 0 0 0 0.1
Bud Neck 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 4.4 3.3
Mitochondria -0.7 -1.5 -1.6 -1.3 -2.2 -1.8 -2.3 -11.0 -10.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 2.5 1.9
Nucleus 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.4 0.7 1.4 0.7 0.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fyv4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available