Standard name
Human Ortholog
Description Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 0.98 0.97 0.95 0.95 0.88 0.88 0.84 0.84 1.0 1.0 0.97 0.98 0.97 0.98 0.97 0.93 0.95 0.9 0.88 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 0 2 2 2 1 0 0 0 0 0 0 0 0 0 0 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 4 6 4 5 2 10 8 6 4 12 0 0 0 12 12 13 0 0 0 0 0 0
Cytoplasm 242 185 280 280 412 337 470 311 313 185 274 28 195 188 343 236 267 233 169 271 27 87 85
Endoplasmic Reticulum 2 1 0 3 3 5 14 15 12 6 19 0 0 0 9 7 5 0 0 2 0 0 0
Endosome 0 0 0 0 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Mitochondria 0 0 0 0 1 1 3 7 11 17 14 0 0 1 1 1 1 1 1 0 0 2 0
Nucleus 0 0 4 0 0 6 6 6 4 7 13 0 0 2 3 3 8 0 2 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 4 7 9 9 18 25 13 13 0 0 3 6 5 0 0 2 2 0 0 0
Unique Cell Count 244 185 281 287 423 353 495 354 357 221 325 28 195 193 349 243 272 241 183 284 31 100 101
Labelled Cell Count 248 190 291 292 430 360 514 367 373 234 347 28 195 195 374 264 294 241 183 284 31 100 101


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 12.6 11.2 10.4 11.7 10.3 10.6 9.0 9.3 8.1 8.5 11.4 11.2 10.8 26.0 26.9 25.4 12.0 14.6
Std Deviation (1e-4) 1.9 2.3 1.8 2.0 4.1 1.7 1.8 1.6 1.7 1.5 1.6 2.4 2.1 2.2 5.7 6.3 5.9 3.7 5.4
Intensity Change (Log2) -0.11 0.05 -0.12 -0.08 -0.31 -0.28 -0.46 -0.4 0.02 -0.0 -0.05 1.21 1.26 1.18 0.09 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 -0.1 0.1 -0.4 0 1.1 0 0 0 1.0 1.8 1.7
Cytoplasm -2.1 -2.3 -3.2 -3.5 -5.8 -5.9 -6.8 -6.7 0.3 0.8 -2.1 -1.6 -2.4 -1.7
Endoplasmic Reticulum 0 0 0 2.8 3.5 3.1 0 4.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 4.7 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1.9 0 0 0 0 0 1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.5 4.2 3.7 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6723 8.7209 7.7011 6.9496 7.4027 7.9663 4.9683 7.2637 6.363 6.2364 5.7949 6.8652 7.0181 9.7343 8.1461 9.4155 7.1143 8.9254
Actin 0.0083 0.0003 0.0063 0.0015 0.0017 0.0016 0.0155 0.0006 0.0067 0.0005 0.0225 0.0115 0.0224 0.0013 0.0073 0.0097 0.0001 0.0024
Bud 0.0006 0.0002 0.0005 0.0005 0.0003 0.0002 0.0003 0.0076 0.0003 0.0001 0.009 0.0005 0.0011 0.0008 0.004 0.0007 0.0004 0.0008
Bud Neck 0.0035 0.0002 0.0008 0.0323 0.0015 0.0009 0.0014 0.0001 0.0004 0.0004 0.001 0.0009 0.0059 0.0004 0.0009 0.0007 0.0006 0.0015
Bud Periphery 0.0006 0 0.0003 0.0004 0.0002 0.0001 0.0003 0.0027 0.0003 0 0.0108 0.0001 0.0008 0.0003 0.0028 0.0007 0.0001 0.0003
Bud Site 0.0042 0.0003 0.0015 0.0009 0.0006 0.0001 0.0016 0.0015 0.0005 0.0001 0.0234 0.0001 0.004 0.0055 0.0022 0.0011 0.0002 0.0013
Cell Periphery 0.0002 0 0.0001 0.0001 0 0 0.0002 0.0001 0.0001 0 0.0004 0 0.0002 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.8524 0.9818 0.9325 0.9252 0.9257 0.9507 0.8443 0.9743 0.9375 0.9833 0.6466 0.9445 0.9002 0.975 0.8892 0.8661 0.876 0.975
Cytoplasmic Foci 0.029 0.003 0.0108 0.0108 0.0107 0.0049 0.0254 0.002 0.0075 0.0039 0.0635 0.004 0.008 0.0022 0.0162 0.0095 0.0123 0.0053
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0003 0 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0035 0.0006 0.0015 0.0011 0.0029 0.0012 0.0116 0.0006 0.0033 0.0016 0.0039 0.0027 0.0029 0.0009 0.0025 0.0034 0.0028 0.0008
Endosome 0.0226 0.001 0.0083 0.0047 0.0187 0.0032 0.0226 0.0015 0.0057 0.0013 0.0415 0.0053 0.0101 0.0037 0.0215 0.0311 0.0785 0.0027
Golgi 0.0082 0.0001 0.0041 0.0029 0.0052 0.0006 0.0048 0.0002 0.0017 0.0001 0.0136 0.0012 0.0031 0.001 0.0069 0.0054 0.0043 0.0003
Lipid Particles 0.0047 0 0.0018 0.0004 0.0015 0.0001 0.0092 0 0.0032 0 0.008 0.0009 0.0038 0.0001 0.0064 0.0059 0.0006 0.0001
Mitochondria 0.0102 0.0001 0.0055 0.0006 0.0112 0.015 0.0024 0.0001 0.0056 0.0001 0.0415 0.0003 0.0035 0.0005 0.0112 0.0339 0.0014 0.0004
None 0.0139 0.004 0.0104 0.0056 0.0024 0.0026 0.0311 0.0059 0.0052 0.0031 0.0038 0.0074 0.0074 0.0018 0.003 0.003 0.0055 0.0016
Nuclear Periphery 0.0028 0.0008 0.0015 0.0011 0.0014 0.0014 0.0083 0.0003 0.0049 0.0013 0.0116 0.0036 0.0069 0.0006 0.0036 0.0038 0.001 0.0005
Nucleolus 0.0009 0 0 0.0002 0.0001 0 0.0005 0 0.0004 0 0.0012 0.0001 0.0003 0.0001 0.0005 0.0002 0.0001 0
Nucleus 0.0144 0.0062 0.0096 0.0079 0.0099 0.0154 0.0076 0.0019 0.0074 0.0033 0.0048 0.0143 0.0106 0.0048 0.0059 0.0083 0.0036 0.0056
Peroxisomes 0.0092 0.0004 0.0021 0.0019 0.0033 0.0006 0.0043 0 0.0031 0.0001 0.0208 0.0003 0.0039 0.0001 0.0089 0.0118 0.0002 0.0005
Punctate Nuclear 0.0043 0.0004 0.0007 0.0005 0.0008 0.0006 0.0033 0.0001 0.0045 0.0004 0.0625 0.0016 0.0019 0.0003 0.004 0.0013 0.0006 0.0004
Vacuole 0.0054 0.0005 0.0013 0.0012 0.0011 0.0007 0.0038 0.0004 0.0013 0.0002 0.0072 0.0006 0.0019 0.0006 0.0018 0.0024 0.0095 0.0003
Vacuole Periphery 0.0008 0.0001 0.0004 0.0002 0.0004 0.0002 0.0015 0.0001 0.0005 0.0001 0.0024 0.0002 0.0012 0.0001 0.0011 0.0008 0.0022 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 164.2546 119.1943 70.0742 67.4184 98.5477 119.0991 89.3691 67.7842 62.766 97.6681
Translational Efficiency 1.6154 1.712 1.4528 1.3753 1.5358 1.5874 1.6076 1.3865 1.2231 1.3621

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
98 866 328 96 1848 1970 1503 1293 1946 2836 1831 1389

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 776.26 1033.91 1415.65 1608.82 1030.59 1060.43 1333.15 1399.01 1017.78 1052.33 1347.93 1413.51
Standard Deviation 112.83 221.43 203.31 309.73 154.90 178.20 573.19 316.66 162.85 192.82 527.35 320.64
Intensity Change Log 2 0.413499 0.866853 1.051391 0.041179 0.371369 0.440936 0.213019 0.605325 0.735246

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000313 0.000368 0.000932 0.021090 0.000120 0.000436 0.000746 0.002921 0.000130 0.000415 0.000779 0.004177
Bud Neck 0.002779 0.000680 0.003209 0.020001 0.000864 0.002051 0.003852 0.011017 0.000960 0.001632 0.003737 0.011638
Bud Site 0.003201 0.005469 0.004895 0.197462* 0.003897 0.007272 0.015977 0.044583 0.003862 0.006722 0.013992 0.055149
Cell Periphery 0.000254 0.000079 0.000281 0.000412 0.000074 0.000049 0.000117 0.000252 0.000083 0.000058 0.000147 0.000263
Cytoplasm 0.578966* 0.844647* 0.487069* 0.354365* 0.812784* 0.795741* 0.680965* 0.432809* 0.801009* 0.810675* 0.646231* 0.427387*
Cytoplasmic Foci 0.175567 0.025665 0.009297 0.033058 0.055340 0.037386 0.015954 0.035097 0.061394 0.033807 0.014761 0.034956
Eisosomes 0.000093 0.000011 0.000015 0.000053 0.000016 0.000013 0.000014 0.000061 0.000019 0.000013 0.000014 0.000060
Endoplasmic Reticulum 0.001644 0.000765 0.002054 0.002564 0.001568 0.000569 0.001619 0.001275 0.001572 0.000629 0.001697 0.001364
Endosome 0.006804 0.002040 0.001450 0.007047 0.002850 0.004849 0.004732 0.031439 0.003049 0.003992 0.004144 0.029753
Golgi 0.001993 0.000291 0.000373 0.000761 0.000459 0.002411 0.006075 0.018214 0.000536 0.001764 0.005054 0.017007
Lipid Particles 0.003030 0.000625 0.001272 0.006246 0.000623 0.001208 0.001804 0.004762 0.000744 0.001030 0.001708 0.004864
Mitochondria 0.008463 0.000096 0.000456 0.000303 0.000493 0.001314 0.000726 0.025025 0.000895 0.000942 0.000678 0.023316
Mitotic Spindle 0.000807 0.003609 0.004195 0.055893 0.000650 0.007745 0.041403 0.097963 0.000658 0.006482 0.034737 0.095056
None 0.002554 0.008433 0.006585 0.008170 0.003951 0.004148 0.008565 0.015152 0.003881 0.005457 0.008211 0.014670
Nuclear Periphery 0.000459 0.000138 0.000823 0.000444 0.000140 0.000244 0.000611 0.000426 0.000156 0.000212 0.000649 0.000428
Nuclear Periphery Foci 0.001121 0.003827 0.003701 0.004701 0.002469 0.001643 0.004885 0.002404 0.002401 0.002310 0.004673 0.002563
Nucleolus 0.000383 0.000274 0.000185 0.000211 0.000211 0.000420 0.000240 0.002524 0.000219 0.000375 0.000230 0.002364
Nucleus 0.147875* 0.059046 0.409586* 0.057236 0.056625 0.066470 0.121419 0.062342 0.061220 0.064203 0.173040* 0.061989
Peroxisomes 0.000937 0.000546 0.000137 0.019836 0.000221 0.002302 0.001992 0.013117 0.000257 0.001766 0.001660 0.013581
Vacuole 0.057450 0.043183 0.063314 0.209835* 0.055993 0.062990 0.087672 0.192544 0.056067 0.056941 0.083309 0.193739
Vacuole Periphery 0.005307 0.000207 0.000171 0.000311 0.000654 0.000738 0.000634 0.006074 0.000888 0.000576 0.000551 0.005675

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.32 -5.22 -1.31 -1.29 -0.89 -2.12 -6.74 -4.42 -2.11 -0.77 -2.70 -9.40 -3.89 -2.56 -0.87
Bud Neck 6.14 -4.70 -2.54 -3.16 -1.65 -4.10 -7.49 -8.76 -4.97 -1.96 -3.22 -9.59 -8.83 -6.83 -2.05
Bud Site -2.58 -5.88 -4.00 -3.78 -3.56 -3.15 -6.46 -8.54 -6.38 -3.17 -3.40 -6.98 -9.37 -7.72 -4.61
Cell Periphery 5.15 -4.15 -1.33 -4.16 1.15 3.89 -5.19 -6.21 -10.13 -1.59 3.85 -9.56 -6.35 -9.86 2.19
Cytoplasm -9.05 5.80 5.49 16.27 1.28 3.14 25.05 31.48 27.98 8.42 -0.40 29.27 31.92 32.92 6.32
Cytoplasmic Foci 8.76 9.74 8.53 -0.26 -4.09 7.15 20.74 18.24 10.13 -3.18 11.03 22.41 19.39 9.95 -4.25
Eisosomes 7.24 6.13 5.45 -3.49 -0.82 1.59 -0.11 -2.55 -3.33 -2.54 4.79 1.84 -1.13 -4.08 -2.25
Endoplasmic Reticulum 2.72 -2.07 2.05 -0.31 4.82 2.25 0.85 2.36 0.30 4.39 2.29 0.20 2.47 0.72 6.84
Endosome 3.11 2.89 2.24 -1.59 -1.09 -2.74 0.76 -3.21 -1.75 -3.43 -1.33 1.52 -3.01 -2.41 -3.52
Golgi 1.12 0.95 1.06 -1.46 3.31 -3.51 -1.66 -3.38 -1.23 -1.23 -2.96 -1.56 -3.25 -1.83 -1.43
Lipid Particles 5.14 1.22 -1.86 -4.18 -2.52 -1.72 -6.92 -5.17 -3.05 -1.07 -1.07 -7.78 -5.60 -4.39 -1.47
Mitochondria 1.02 0.97 1.02 -1.22 10.23 -1.46 0.19 -2.91 -2.35 -2.98 0.09 0.89 -2.44 -2.59 -2.95
Mitotic Spindle -2.42 -4.35 -2.72 -2.29 -2.00 -4.43 -9.78 -10.24 -8.10 -1.91 -5.02 -10.04 -10.49 -8.72 -2.82
None -3.62 -2.56 0.03 2.78 1.76 -0.20 -2.90 -2.08 -2.08 -0.36 -2.31 -3.20 -2.05 -1.03 -0.29
Nuclear Periphery 3.60 -7.03 -1.72 -5.88 5.00 -1.68 -19.08 -17.08 -8.61 2.82 -1.27 -22.09 -17.12 -11.69 5.08
Nuclear Periphery Foci -7.10 -8.25 -4.49 -1.07 0.92 3.07 -5.08 1.87 -1.86 7.06 0.36 -6.84 0.68 0.26 8.04
Nucleolus 2.20 2.45 2.14 0.55 0.67 -2.42 -1.06 -1.76 -0.86 -1.48 -2.06 -0.90 -1.71 -1.05 -1.49
Nucleus 5.11 -12.51 5.09 1.08 23.10 -4.75 -21.11 -6.71 -2.72 13.19 -2.18 -26.37 -4.67 -3.11 21.39
Peroxisomes 2.03 4.10 -1.60 -1.98 -2.21 -3.89 -2.80 -4.15 -0.66 -2.31 -3.96 -2.63 -4.39 -1.60 -2.83
Vacuole 0.10 -4.77 -12.38 -13.09 -10.22 -3.26 -18.57 -29.01 -26.72 -11.94 -2.52 -19.26 -31.05 -29.85 -14.79
Vacuole Periphery 1.06 1.05 1.06 -0.22 0.73 -0.12 1.42 -1.37 -1.48 -2.83 1.02 1.77 -0.50 -1.93 -2.85