Standard name
Description Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0.06 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.11 0.25 0.26 0.27 – 0.14 0.21 0.08 0.12 0.12 0.27 0.36 0.16 0.11 0.21 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.2 – 0.05 0.15 0.15 0.08 – 0 0 0 0 0 0.16 0.2 0.3 0.35 0.33 0.14 0.09 0.16 0.07 0.14 0.21
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.05 – 0.05 0 0.17 0.19 – 0.63 0.38 0.81 0.64 0.08 0 0 0.19 0.12 0.19 0 0 0 0 0 0
Nucleus 0.29 0.33 – 0.74 0.18 0.17 0.23 – 0.23 0.24 0.24 0.21 0.33 0.27 0.16 0.13 0.16 0.16 0.1 0.09 0.09 0.09 0.07 0.05
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.55 0.46 – 0 0 0 0 – 0 0.05 0 0 0.46 0.07 0 0.32 0.35 0.26 0.31 0.4 0.39 0.35 0.21 0.16
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.22 0.33 – 0.26 0.25 0.2 0.29 – 0.15 0.29 0.08 0.18 0 0.37 0.32 0.34 0.35 0.29 0.36 0.35 0.2 0.37 0.36 0.26
Vac/Vac Membrane 0 0 – 0 0.13 0.15 0 – 0 0 0.07 0 0 0 0.05 0.11 0.14 0.16 0.05 0 0.06 0 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 2 3 – 2 6 1 4 0 0 0 1 1 0 0 0 1 0 2 0
Bud Neck 0 0 – 0 7 4 10 – 0 4 0 5 0 0 3 0 1 0 0 0 0 0 3 2
Bud Site 0 0 – 0 0 4 5 – 1 4 5 6 0 0 0 1 1 1 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Cytoplasm 0 6 – 2 28 33 46 – 10 28 9 19 3 34 31 19 8 12 0 0 1 2 4 9
Endoplasmic Reticulum 1 0 – 0 5 0 4 – 0 0 1 5 0 2 1 4 0 2 0 0 0 0 1 0
Endosome 2 41 – 1 17 19 13 – 2 6 4 6 1 20 17 35 26 19 11 22 7 4 26 29
Golgi 0 1 – 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 2 0 0 1 0
Mitochondria 30 10 – 1 4 22 33 – 46 51 92 103 2 1 0 22 9 11 0 0 0 1 4 3
Nucleus 20 68 – 14 20 22 39 – 17 33 27 33 8 34 14 15 12 9 7 23 4 5 12 7
Nuclear Periphery 1 0 – 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 2
Nucleolus 38 94 – 0 0 1 6 – 1 7 2 2 11 9 1 37 26 15 24 99 18 23 38 22
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0
SpindlePole 15 68 – 5 28 26 49 – 11 39 9 29 1 46 28 39 26 17 28 88 9 24 67 36
Vac/Vac Membrane 2 2 – 0 15 19 5 – 3 4 8 3 0 4 4 13 10 9 4 3 2 0 17 13
Unique Cell Count 69 205 19 114 128 171 73 135 113 160 24 125 87 116 74 58 80 249 47 66 186 140
Labelled Cell Count 109 290 23 124 152 213 93 183 158 216 26 151 100 188 122 97 80 249 47 66 186 140


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.8 – 5.6 5.7 4.5 4.7 – 3.7 4.2 3.6 3.6 5.2 5.6 5.3 7.7 7.5 7.4 – 5.5 5.5
Std Deviation (1e-4) 0.5 0.9 – 2.1 1.7 1.4 2.1 – 1.0 1.3 1.3 1.0 0.7 1.5 1.3 2.3 1.8 2.5 – 1.2 1.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –