Standard name
Description Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0.10
Bud 0 0 0 0.05 0 0 0 0.05 0.05 0.06 0.08 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 0.11 0.12 0 0 0 0 0 0.07 0.07
Cytoplasm 0.89 0.90 0.89 0.85 0.85 0.81 0.81 0.75 0.70 0.72 0.65 0.68 1.00 0.93 0.96 0.34 0.39 0.07 0.18 0.24 0.15
Endoplasmic Reticulum 0.10 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.34 0.28 0.58 0.31 0.30 0.32
Endosome 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.13 0.17 0.23 0.32 0.33 0.46 0.34 0 0 0 0 0 0.09 0.23 0.14 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.14 0.10 0.11 0.08 0.10
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 4 3 2 3 0 2 2 1 1 6 0 0 0 9 5 18 3 14 24
Bud 2 1 5 11 5 9 11 22 15 29 29 21 0 0 2 3 0 2 1 0 3
Bud Neck 1 0 0 0 0 0 0 0 0 0 1 0 0 1 4 13 10 6 5 4 10
Bud Site 0 0 0 0 0 1 0 0 1 2 1 1 0 0 0
Cell Periphery 5 4 11 13 16 19 14 8 14 13 11 16 4 15 9 2 3 8 5 10 16
Cytoplasm 311 165 262 202 289 249 312 359 220 364 239 260 36 116 422 139 77 21 21 36 36
Endoplasmic Reticulum 36 15 11 4 9 16 10 11 5 7 4 14 1 4 27 135 56 175 36 45 75
Endosome 0 1 12 10 16 8 14 27 6 8 3 6 0 0 3 2 3 3 0 4 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 3 0 1 2
Mitochondria 0 4 2 11 11 40 66 112 102 165 171 131 0 0 7 17 7 25 27 22 25
Nucleus 0 0 0 2 0 2 1 1 2 1 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 1 0 1 3 2 1 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 2 6 16 1 0 2 2 4 1 2 0 0 1 0 0 1 0 1 4
SpindlePole 0 0 3 1 4 0 2 1 1 5 1 2 0 0 3 1 0 0 0 0 0
Vac/Vac Membrane 3 0 5 1 13 12 15 6 4 5 2 7 0 0 4 75 27 31 12 11 23
Unique Cell Count 348 183 294 237 339 308 386 481 314 506 369 382 36 125 440 403 200 303 119 155 235
Labelled Cell Count 360 193 318 265 381 362 445 552 378 606 466 468 41 137 486 403 200 303 119 155 235

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Endoplasmic Reticulum

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.4 5.5 5.4 5.7 4.7 4.4 4.7 4.3 4.1 4.0 4.1 5.6 5.9 4.7 4.8 6.1 5.7 6.4 6.8
Std Deviation (1e-4) 0.6 0.8 1.8 1.4 2.2 1.4 0.9 1.6 1.2 1.1 1.3 0.8 0.9 0.5 1.3 1.3 1.9 1.6 1.5 1.5
Intensity Change (Log2) -0.0 0.1 -0.2 -0.3 -0.2 -0.3 -0.4 -0.5 -0.4 0.0 0.1 -0.2 -0.2 0.2 0.0 0.2 0.3

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2.0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 0.6 0 0 0 0 0 0 0 0 3.2 -1.4
Cytoplasm -0.4 0.9 0 0 0 0 0 0 0 -1.8 0.1 -2.2
Endoplasmic Reticulum -1.4 -0.8 0 0 0 0 0 0 0 0 0 1.4
Endosome 0.1 0.4 0 0 0 0 0 0 0 0 0 -3.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.9 2.3 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 3.0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0