Standard name
Human Ortholog
Description Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.07 0.08 0.07 0.07 0.07 0 0.05 0.06 0 0 0.09 0.12 0.07 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.1 0.21 0.23 0.48 0.35 0.45 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.95 0.93 0.91 0.89 0.88 0.85 0.87 0.82 0.81 0.79 0.84 0.96 0.89 0.82 0.85 0.8 0.85 0.95 0.9 0.92 0.89 0.77 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0 0 0 0.05 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.06 0 0 0 0.05 0.12 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 3 3 3 6 1 0 0 0 0 0 1 0 0 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 5 1 6 9 7 10 10 0 0 0 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 2 7 22 14 23 20 9 15 13 10 5 19 31 17 7 15 0 3 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 2 0 0 0 1 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 3 0 0 0 0 0 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 2
Mitochondria 13 1 0 2 6 21 67 63 145 113 102 72 2 0 1 6 1 3 1 1 0 1 1 10
Nucleus 222 96 93 88 250 186 276 243 250 259 178 202 176 183 219 204 103 176 216 107 102 141 216 165
Nuclear Periphery 3 1 0 0 3 2 6 1 4 1 4 2 1 0 7 0 2 0 0 0 1 0 0 3
Nucleolus 1 1 3 7 3 4 10 13 19 21 15 9 3 2 12 3 3 9 2 0 0 2 17 18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 1 3 1 1 3 1 1 5 1 1 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 3 0 6 6 3 1 2 1 1 0 0 0 8 11 11 13 5 2 3 7 34 40
Unique Cell Count 230 101 100 97 280 212 326 278 304 320 226 241 183 205 267 240 128 208 228 119 111 159 282 251
Labelled Cell Count 239 104 101 104 291 238 388 352 445 421 330 310 188 205 287 248 134 220 228 119 111 159 282 251


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 8.7 7.1 6.4 7.0 5.9 5.3 5.3 4.3 4.9 4.3 4.9 8.0 8.4 7.9 11.8 11.0 10.9 8.6 8.9 8.9
Std Deviation (1e-4) 0.9 1.5 1.7 1.3 2.2 2.3 1.9 1.5 1.1 1.6 1.1 1.4 1.8 2.8 2.6 3.3 2.9 2.7 1.8 1.7 1.5
Intensity Change (Log2) -0.15 -0.01 -0.26 -0.42 -0.43 -0.71 -0.53 -0.71 -0.52 0.18 0.24 0.16 0.74 0.64 0.61 0.28 0.32 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.1 1.7 1.9 1.9 0 0 0 0 0 2.4 2.9 1.9 0 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 4.9 5.2 0 7.0 0 6.2 0 0 0 0 0 0
Nucleus -0.6 -1.1 -1.4 -2.1 -1.5 -2.6 -2.9 -3.2 -2.3 1.2 -1.0 -2.6 -2.0 -2.7 -2.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1.3 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.7 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3409 3.201 3.0274 2.7081 3.1292 3.0291 3.8055 4.7361 3.8709 4.0837 4.271 4.1475 3.9466 3.8654 3.7202 3.4104 3.0402 3.6171
Actin 0.0207 0 0.0043 0 0.0267 0.0002 0.0053 0 0.0107 0.0027 0.0027 0.0005 0.0134 0 0.0104 0.0492 0.0082 0.0072
Bud 0.0005 0 0.0001 0 0.0003 0 0.0007 0 0.0005 0.0002 0.001 0.0001 0.0006 0 0.0002 0.0011 0.001 0
Bud Neck 0.0073 0.0001 0.0008 0 0.0004 0.0003 0.0034 0.0001 0.0011 0.0002 0.0002 0.0008 0.002 0 0.0014 0.002 0.0013 0.0003
Bud Periphery 0.0012 0 0.0004 0 0.0005 0.0001 0.0024 0 0.0009 0.0002 0.0018 0.0003 0.0022 0 0.0005 0.0011 0.0026 0.0001
Bud Site 0.0029 0 0.0005 0 0.0009 0 0.0036 0 0.0062 0.0012 0.001 0.0001 0.006 0 0.0008 0.0294 0.0033 0
Cell Periphery 0.0005 0 0.0002 0 0.0001 0 0.0013 0 0.0002 0 0.0001 0.0001 0.0016 0 0.0002 0.0002 0.0006 0
Cytoplasm 0.0063 0 0.0007 0 0.0005 0.0001 0.0029 0 0.0018 0.0137 0.0004 0.0002 0.0032 0 0.0013 0.0004 0.0009 0
Cytoplasmic Foci 0.0107 0 0.0015 0 0.0068 0.0001 0.0114 0 0.0049 0.005 0.0018 0.0003 0.0104 0.0019 0.0028 0.0022 0.0201 0.0006
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.002 0 0.0018 0 0.0008 0.0006 0.0022 0 0.0015 0.0012 0.0009 0.0005 0.0017 0 0.0018 0.0002 0.0061 0.0001
Endosome 0.0133 0 0.0085 0 0.0101 0.0019 0.0146 0 0.013 0.0201 0.0336 0.0059 0.0109 0.0001 0.0048 0.0007 0.0186 0.0009
Golgi 0.0045 0 0.0022 0 0.0078 0.0001 0.0034 0 0.0038 0.0043 0.0089 0.0012 0.0032 0.0002 0.0016 0.0012 0.0165 0.0011
Lipid Particles 0.0099 0 0.0035 0 0.0089 0.0001 0.0171 0 0.0105 0.0037 0.0029 0.0019 0.0125 0.0051 0.0039 0.001 0.0165 0.0013
Mitochondria 0.0041 0 0.0019 0.0001 0.0034 0.0002 0.0274 0.0001 0.0082 0.0018 0.0779 0.0009 0.0168 0.0001 0.0098 0.0008 0.0509 0.0076
None 0.0094 0 0.0008 0 0.0006 0.0001 0.0051 0 0.001 0.0025 0.0002 0.0002 0.0015 0 0.008 0.0004 0.0004 0.0001
Nuclear Periphery 0.0226 0.0007 0.0114 0.0011 0.0253 0.0138 0.01 0.0011 0.0119 0.005 0.0019 0.0056 0.0047 0.0008 0.008 0.0007 0.0215 0.0003
Nucleolus 0.0169 0.0063 0.0089 0.0022 0.0139 0.0222 0.0218 0.0073 0.0068 0.0058 0.0092 0.034 0.0135 0.0115 0.009 0.0077 0.0231 0.0227
Nucleus 0.846 0.9927 0.9449 0.9964 0.8748 0.9588 0.8368 0.9911 0.8981 0.9261 0.848 0.9427 0.8722 0.9751 0.9288 0.8965 0.7802 0.954
Peroxisomes 0.0124 0 0.0004 0 0.0059 0 0.0076 0 0.0097 0.0017 0.0009 0.0001 0.0128 0.0048 0.003 0.0012 0.0169 0.003
Punctate Nuclear 0.0038 0.0001 0.0021 0.0001 0.0099 0.0005 0.0086 0.0002 0.0044 0.0028 0.0003 0.0033 0.0023 0.0002 0.0018 0.0032 0.0016 0.0003
Vacuole 0.0029 0.0001 0.0037 0 0.0009 0.0004 0.0101 0 0.003 0.0012 0.0035 0.0008 0.0059 0 0.0011 0.0005 0.0059 0.0001
Vacuole Periphery 0.002 0 0.0014 0 0.0015 0.0004 0.0043 0 0.0014 0.0005 0.0026 0.0006 0.0024 0 0.0007 0.0001 0.0038 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3114 20.6108 14.5658 16.5712 27.7581 7.6811 19.103 16.7444 11.7122 18.1533
Translational Efficiency 1.5997 1.4874 1.8056 1.1784 0.9616 2.7629 0.9924 0.9688 1.2541 1.4951

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2171 669 282 1900 1603 1660 353 1351 3774 2329 635 3251

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 936.92 883.73 1207.73 1196.45 823.95 972.37 1272.98 1222.04 888.94 946.91 1244.00 1207.08
Standard Deviation 137.49 200.59 159.35 194.91 147.77 145.00 158.56 199.47 152.54 167.78 162.18 197.22
Intensity Change Log 2 -0.084320 0.366300 0.352762 0.238949 0.627581 0.568663 0.075986 0.494465 0.457818

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000209 0.000689 0.000856 0.000723 0.000365 0.000445 0.000751 0.000965 0.000275 0.000515 0.000797 0.000823
Bud Neck 0.002764 0.003765 0.010856 0.019565 0.003498 0.009287 0.008473 0.023361 0.003076 0.007701 0.009531 0.021143
Bud Site 0.000232 0.000976 0.000735 0.001634 0.000495 0.001375 0.000535 0.003462 0.000343 0.001260 0.000624 0.002394
Cell Periphery 0.000306 0.000352 0.000037 0.000076 0.000340 0.000208 0.000029 0.000445 0.000321 0.000249 0.000033 0.000229
Cytoplasm 0.000144 0.004578 0.000135 0.000711 0.001879 0.000551 0.000099 0.001392 0.000881 0.001708 0.000115 0.000994
Cytoplasmic Foci 0.000006 0.000816 0.000017 0.000156 0.000093 0.000520 0.000010 0.002407 0.000043 0.000605 0.000013 0.001091
Eisosomes 0.000042 0.000036 0.000044 0.000030 0.000042 0.000040 0.000043 0.000028 0.000042 0.000039 0.000044 0.000029
Endoplasmic Reticulum 0.007357 0.006274 0.003176 0.002995 0.008283 0.007321 0.003520 0.003072 0.007750 0.007020 0.003367 0.003027
Endosome 0.000122 0.000435 0.000067 0.000509 0.000225 0.000815 0.000051 0.002064 0.000166 0.000706 0.000058 0.001155
Golgi 0.000012 0.000297 0.000053 0.000463 0.000101 0.000404 0.000038 0.003111 0.000050 0.000373 0.000045 0.001563
Lipid Particles 0.000014 0.000155 0.000042 0.000054 0.000136 0.000777 0.000031 0.000085 0.000066 0.000598 0.000036 0.000067
Mitochondria 0.000342 0.001537 0.001299 0.004269 0.001070 0.001532 0.001005 0.004836 0.000651 0.001533 0.001136 0.004505
Mitotic Spindle 0.000235 0.001944 0.000058 0.004901 0.000171 0.003743 0.000117 0.007712 0.000208 0.003226 0.000091 0.006070
None 0.001145 0.022859 0.000919 0.001369 0.005703 0.001584 0.000804 0.001496 0.003081 0.007695 0.000855 0.001422
Nuclear Periphery 0.000211 0.000191 0.000175 0.001257 0.000279 0.000543 0.000166 0.001087 0.000240 0.000442 0.000170 0.001186
Nuclear Periphery Foci 0.000037 0.000738 0.000027 0.000369 0.000259 0.000479 0.000028 0.000572 0.000131 0.000554 0.000027 0.000453
Nucleolus 0.008445 0.016578 0.013828 0.012377 0.010463 0.009631 0.011342 0.013720 0.009302 0.011626 0.012446 0.012935
Nucleus 0.977456* 0.935627* 0.966143* 0.944622* 0.965237* 0.958858* 0.971763* 0.925150* 0.972266* 0.952185* 0.969267* 0.936530*
Peroxisomes 0.000094 0.000638 0.000608 0.000195 0.000327 0.000563 0.000456 0.000523 0.000193 0.000585 0.000524 0.000332
Vacuole 0.000618 0.001053 0.000812 0.001977 0.000821 0.000933 0.000655 0.003368 0.000704 0.000968 0.000724 0.002555
Vacuole Periphery 0.000209 0.000461 0.000112 0.001749 0.000213 0.000392 0.000084 0.001143 0.000211 0.000412 0.000097 0.001497

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.63 -12.49 -13.50 -2.37 0.47 -2.11 -8.56 -5.31 -4.59 -1.53 -8.42 -15.36 -11.00 -6.38 -1.05
Bud Neck -1.99 -8.72 -17.87 -15.73 -7.80 -6.17 -7.64 -13.93 -9.23 -9.66 -6.81 -11.76 -22.39 -14.48 -12.79
Bud Site -3.17 -6.50 -4.01 -2.07 -2.77 -2.88 -1.41 -4.57 -2.92 -4.36 -4.13 -5.71 -6.05 -3.12 -5.08
Cell Periphery -0.33 2.12 1.81 3.92 -3.67 1.88 5.42 -0.08 -0.61 -1.31 0.84 3.73 0.96 0.48 -1.53
Cytoplasm -2.97 0.17 -4.55 2.34 -4.72 2.30 3.26 0.52 -2.20 -3.55 -1.66 3.26 -1.21 0.94 -5.61
Cytoplasmic Foci -1.98 -4.26 -2.44 1.66 -2.22 -1.89 1.91 -2.71 -1.94 -2.85 -2.85 1.64 -2.95 -0.71 -3.08
Eisosomes 1.16 -0.53 2.33 1.75 5.15 0.36 -1.16 3.95 3.98 6.60 0.82 -1.06 3.89 4.20 8.38
Endoplasmic Reticulum 2.10 9.38 11.76 8.05 0.25 1.46 8.10 8.25 8.83 1.29 1.73 12.47 13.82 12.61 1.41
Endosome -2.56 2.90 -2.77 0.04 -3.26 -1.92 4.75 -3.08 -1.48 -3.45 -2.44 5.65 -3.79 -0.73 -4.31
Golgi -1.41 -9.65 -1.88 -0.30 -1.65 -1.59 1.01 -2.51 -2.15 -2.59 -2.31 0.17 -2.94 -2.02 -2.96
Lipid Particles -2.82 -4.44 -3.29 1.77 -1.33 -1.30 0.94 0.52 1.47 -3.12 -1.55 0.63 -0.02 1.56 -3.00
Mitochondria -2.94 -7.69 -5.51 -3.25 -3.98 -0.81 -0.02 -3.42 -3.16 -3.91 -2.58 -2.36 -6.20 -4.52 -5.67
Mitotic Spindle -1.38 0.66 -4.38 -2.31 -4.55 -3.05 0.58 -4.91 -2.64 -4.95 -3.32 0.78 -6.57 -3.04 -6.71
None -4.92 0.65 -1.31 4.82 -2.33 3.73 4.48 3.59 -0.20 -2.40 -3.33 4.39 2.82 4.66 -3.53
Nuclear Periphery 0.61 0.37 -5.64 -5.74 -5.70 -2.68 2.38 -8.05 -4.93 -8.84 -2.91 1.96 -8.56 -6.14 -8.96
Nuclear Periphery Foci -2.88 0.29 -3.52 1.47 -3.75 -0.85 2.09 -1.55 -0.23 -3.50 -2.22 2.07 -3.21 0.60 -5.07
Nucleolus -4.40 -2.49 -4.80 0.71 -0.34 0.54 -0.49 -4.30 -4.35 -2.58 -2.54 -2.17 -6.42 -3.52 -2.06
Nucleus 6.83 4.33 12.16 -0.10 6.81 1.96 -1.49 9.18 7.37 9.77 7.05 2.15 14.84 6.15 11.97
Peroxisomes -2.47 -6.03 -7.14 1.84 4.25 -0.84 -0.53 -0.96 0.04 -0.85 -2.25 -3.27 -1.72 1.37 1.94
Vacuole -1.48 -1.03 -3.49 -2.12 -3.04 -0.81 -0.21 -4.00 -3.77 -3.94 -1.78 -1.03 -5.31 -4.46 -4.98
Vacuole Periphery -1.02 1.33 -2.75 -1.93 -3.00 -1.49 3.57 -3.04 -2.14 -3.56 -1.75 2.55 -3.70 -2.80 -4.15