Standard name | |
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Description | Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 6 | 9 | 7 | 10 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 5 | 2 | 7 | 22 | 14 | 23 | 20 | 9 | 15 | 13 | 10 | 5 | 19 | 31 | 17 | 7 | 15 | 0 | 3 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 13 | 1 | 0 | 2 | 6 | 21 | 67 | 63 | 145 | 113 | 102 | 72 | 2 | 0 | 1 | 6 | 1 | 3 | 1 | 1 | 0 | 1 | 1 | 10 |
Nucleus | 222 | 96 | 93 | 88 | 250 | 186 | 276 | 243 | 250 | 259 | 178 | 202 | 176 | 183 | 219 | 204 | 103 | 176 | 216 | 107 | 102 | 141 | 216 | 165 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 3 | 2 | 6 | 1 | 4 | 1 | 4 | 2 | 1 | 0 | 7 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 3 |
Nucleolus | 1 | 1 | 3 | 7 | 3 | 4 | 10 | 13 | 19 | 21 | 15 | 9 | 3 | 2 | 12 | 3 | 3 | 9 | 2 | 0 | 0 | 2 | 17 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 1 | 3 | 1 | 1 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 3 | 0 | 6 | 6 | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 8 | 11 | 11 | 13 | 5 | 2 | 3 | 7 | 34 | 40 |
Unique Cell Count | 230 | 101 | 100 | 97 | 280 | 212 | 326 | 278 | 304 | 320 | 226 | 241 | 183 | 205 | 267 | 240 | 128 | 208 | 228 | 119 | 111 | 159 | 282 | 251 |
Labelled Cell Count | 239 | 104 | 101 | 104 | 291 | 238 | 388 | 352 | 445 | 421 | 330 | 310 | 188 | 205 | 287 | 248 | 134 | 220 | 228 | 119 | 111 | 159 | 282 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 8.7 | 7.1 | 6.4 | 7.0 | 5.9 | 5.3 | 5.3 | 4.3 | 4.9 | 4.3 | 4.9 | 8.0 | 8.4 | 7.9 | 11.8 | 11.0 | 10.9 | 8.6 | 8.9 | 8.9 |
Std Deviation (1e-4) | 0.9 | 1.5 | 1.7 | 1.3 | 2.2 | 2.3 | 1.9 | 1.5 | 1.1 | 1.6 | 1.1 | 1.4 | 1.8 | 2.8 | 2.6 | 3.3 | 2.9 | 2.7 | 1.8 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.01 | -0.26 | -0.42 | -0.43 | -0.71 | -0.53 | -0.71 | -0.52 | 0.18 | 0.24 | 0.16 | 0.74 | 0.64 | 0.61 | 0.28 | 0.32 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.1 | 1.7 | 1.9 | 1.9 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.9 | 1.9 | 0 | 1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.3 | 4.9 | 5.2 | 0 | 7.0 | 0 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.6 | -1.1 | -1.4 | -2.1 | -1.5 | -2.6 | -2.9 | -3.2 | -2.3 | 1.2 | -1.0 | -2.6 | -2.0 | -2.7 | -2.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 1.2 |