Standard name | |
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Human Ortholog | |
Description | Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 6 | 9 | 7 | 10 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 5 | 2 | 7 | 22 | 14 | 23 | 20 | 9 | 15 | 13 | 10 | 5 | 19 | 31 | 17 | 7 | 15 | 0 | 3 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 13 | 1 | 0 | 2 | 6 | 21 | 67 | 63 | 145 | 113 | 102 | 72 | 2 | 0 | 1 | 6 | 1 | 3 | 1 | 1 | 0 | 1 | 1 | 10 |
Nucleus | 222 | 96 | 93 | 88 | 250 | 186 | 276 | 243 | 250 | 259 | 178 | 202 | 176 | 183 | 219 | 204 | 103 | 176 | 216 | 107 | 102 | 141 | 216 | 165 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 3 | 2 | 6 | 1 | 4 | 1 | 4 | 2 | 1 | 0 | 7 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 3 |
Nucleolus | 1 | 1 | 3 | 7 | 3 | 4 | 10 | 13 | 19 | 21 | 15 | 9 | 3 | 2 | 12 | 3 | 3 | 9 | 2 | 0 | 0 | 2 | 17 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 1 | 3 | 1 | 1 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 3 | 0 | 6 | 6 | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 8 | 11 | 11 | 13 | 5 | 2 | 3 | 7 | 34 | 40 |
Unique Cell Count | 230 | 101 | 100 | 97 | 280 | 212 | 326 | 278 | 304 | 320 | 226 | 241 | 183 | 205 | 267 | 240 | 128 | 208 | 228 | 119 | 111 | 159 | 282 | 251 |
Labelled Cell Count | 239 | 104 | 101 | 104 | 291 | 238 | 388 | 352 | 445 | 421 | 330 | 310 | 188 | 205 | 287 | 248 | 134 | 220 | 228 | 119 | 111 | 159 | 282 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 8.7 | 7.1 | 6.4 | 7.0 | 5.9 | 5.3 | 5.3 | 4.3 | 4.9 | 4.3 | 4.9 | 8.0 | 8.4 | 7.9 | 11.8 | 11.0 | 10.9 | 8.6 | 8.9 | 8.9 |
Std Deviation (1e-4) | 0.9 | 1.5 | 1.7 | 1.3 | 2.2 | 2.3 | 1.9 | 1.5 | 1.1 | 1.6 | 1.1 | 1.4 | 1.8 | 2.8 | 2.6 | 3.3 | 2.9 | 2.7 | 1.8 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.01 | -0.26 | -0.42 | -0.43 | -0.71 | -0.53 | -0.71 | -0.52 | 0.18 | 0.24 | 0.16 | 0.74 | 0.64 | 0.61 | 0.28 | 0.32 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.1 | 1.7 | 1.9 | 1.9 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.9 | 1.9 | 0 | 1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.3 | 4.9 | 5.2 | 0 | 7.0 | 0 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.6 | -1.1 | -1.4 | -2.1 | -1.5 | -2.6 | -2.9 | -3.2 | -2.3 | 1.2 | -1.0 | -2.6 | -2.0 | -2.7 | -2.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3409 | 3.201 | 3.0274 | 2.7081 | 3.1292 | 3.0291 | 3.8055 | 4.7361 | 3.8709 | 4.0837 | 4.271 | 4.1475 | 3.9466 | 3.8654 | 3.7202 | 3.4104 | 3.0402 | 3.6171 |
Actin | 0.0207 | 0 | 0.0043 | 0 | 0.0267 | 0.0002 | 0.0053 | 0 | 0.0107 | 0.0027 | 0.0027 | 0.0005 | 0.0134 | 0 | 0.0104 | 0.0492 | 0.0082 | 0.0072 |
Bud | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0005 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0011 | 0.001 | 0 |
Bud Neck | 0.0073 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0003 | 0.0034 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0008 | 0.002 | 0 | 0.0014 | 0.002 | 0.0013 | 0.0003 |
Bud Periphery | 0.0012 | 0 | 0.0004 | 0 | 0.0005 | 0.0001 | 0.0024 | 0 | 0.0009 | 0.0002 | 0.0018 | 0.0003 | 0.0022 | 0 | 0.0005 | 0.0011 | 0.0026 | 0.0001 |
Bud Site | 0.0029 | 0 | 0.0005 | 0 | 0.0009 | 0 | 0.0036 | 0 | 0.0062 | 0.0012 | 0.001 | 0.0001 | 0.006 | 0 | 0.0008 | 0.0294 | 0.0033 | 0 |
Cell Periphery | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0013 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0016 | 0 | 0.0002 | 0.0002 | 0.0006 | 0 |
Cytoplasm | 0.0063 | 0 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0029 | 0 | 0.0018 | 0.0137 | 0.0004 | 0.0002 | 0.0032 | 0 | 0.0013 | 0.0004 | 0.0009 | 0 |
Cytoplasmic Foci | 0.0107 | 0 | 0.0015 | 0 | 0.0068 | 0.0001 | 0.0114 | 0 | 0.0049 | 0.005 | 0.0018 | 0.0003 | 0.0104 | 0.0019 | 0.0028 | 0.0022 | 0.0201 | 0.0006 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.002 | 0 | 0.0018 | 0 | 0.0008 | 0.0006 | 0.0022 | 0 | 0.0015 | 0.0012 | 0.0009 | 0.0005 | 0.0017 | 0 | 0.0018 | 0.0002 | 0.0061 | 0.0001 |
Endosome | 0.0133 | 0 | 0.0085 | 0 | 0.0101 | 0.0019 | 0.0146 | 0 | 0.013 | 0.0201 | 0.0336 | 0.0059 | 0.0109 | 0.0001 | 0.0048 | 0.0007 | 0.0186 | 0.0009 |
Golgi | 0.0045 | 0 | 0.0022 | 0 | 0.0078 | 0.0001 | 0.0034 | 0 | 0.0038 | 0.0043 | 0.0089 | 0.0012 | 0.0032 | 0.0002 | 0.0016 | 0.0012 | 0.0165 | 0.0011 |
Lipid Particles | 0.0099 | 0 | 0.0035 | 0 | 0.0089 | 0.0001 | 0.0171 | 0 | 0.0105 | 0.0037 | 0.0029 | 0.0019 | 0.0125 | 0.0051 | 0.0039 | 0.001 | 0.0165 | 0.0013 |
Mitochondria | 0.0041 | 0 | 0.0019 | 0.0001 | 0.0034 | 0.0002 | 0.0274 | 0.0001 | 0.0082 | 0.0018 | 0.0779 | 0.0009 | 0.0168 | 0.0001 | 0.0098 | 0.0008 | 0.0509 | 0.0076 |
None | 0.0094 | 0 | 0.0008 | 0 | 0.0006 | 0.0001 | 0.0051 | 0 | 0.001 | 0.0025 | 0.0002 | 0.0002 | 0.0015 | 0 | 0.008 | 0.0004 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0226 | 0.0007 | 0.0114 | 0.0011 | 0.0253 | 0.0138 | 0.01 | 0.0011 | 0.0119 | 0.005 | 0.0019 | 0.0056 | 0.0047 | 0.0008 | 0.008 | 0.0007 | 0.0215 | 0.0003 |
Nucleolus | 0.0169 | 0.0063 | 0.0089 | 0.0022 | 0.0139 | 0.0222 | 0.0218 | 0.0073 | 0.0068 | 0.0058 | 0.0092 | 0.034 | 0.0135 | 0.0115 | 0.009 | 0.0077 | 0.0231 | 0.0227 |
Nucleus | 0.846 | 0.9927 | 0.9449 | 0.9964 | 0.8748 | 0.9588 | 0.8368 | 0.9911 | 0.8981 | 0.9261 | 0.848 | 0.9427 | 0.8722 | 0.9751 | 0.9288 | 0.8965 | 0.7802 | 0.954 |
Peroxisomes | 0.0124 | 0 | 0.0004 | 0 | 0.0059 | 0 | 0.0076 | 0 | 0.0097 | 0.0017 | 0.0009 | 0.0001 | 0.0128 | 0.0048 | 0.003 | 0.0012 | 0.0169 | 0.003 |
Punctate Nuclear | 0.0038 | 0.0001 | 0.0021 | 0.0001 | 0.0099 | 0.0005 | 0.0086 | 0.0002 | 0.0044 | 0.0028 | 0.0003 | 0.0033 | 0.0023 | 0.0002 | 0.0018 | 0.0032 | 0.0016 | 0.0003 |
Vacuole | 0.0029 | 0.0001 | 0.0037 | 0 | 0.0009 | 0.0004 | 0.0101 | 0 | 0.003 | 0.0012 | 0.0035 | 0.0008 | 0.0059 | 0 | 0.0011 | 0.0005 | 0.0059 | 0.0001 |
Vacuole Periphery | 0.002 | 0 | 0.0014 | 0 | 0.0015 | 0.0004 | 0.0043 | 0 | 0.0014 | 0.0005 | 0.0026 | 0.0006 | 0.0024 | 0 | 0.0007 | 0.0001 | 0.0038 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.3114 | 20.6108 | 14.5658 | 16.5712 | 27.7581 | 7.6811 | 19.103 | 16.7444 | 11.7122 | 18.1533 |
Translational Efficiency | 1.5997 | 1.4874 | 1.8056 | 1.1784 | 0.9616 | 2.7629 | 0.9924 | 0.9688 | 1.2541 | 1.4951 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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