Standard name
Description Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.07 0.08 0.07 0.07 0.07 0 0.05 0.06 0 0 0.09 0.12 0.07 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.1 0.21 0.23 0.48 0.35 0.45 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.95 0.93 0.91 0.89 0.88 0.85 0.87 0.82 0.81 0.79 0.84 0.96 0.89 0.82 0.85 0.8 0.85 0.95 0.9 0.92 0.89 0.77 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0 0 0 0.05 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.06 0 0 0 0.05 0.12 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 3 3 3 6 1 0 0 0 0 0 1 0 0 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 5 1 6 9 7 10 10 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 2 7 22 14 23 20 9 15 13 10 5 19 31 17 7 15 0 3 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 2 0 0 0 1 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 3 0 0 0 0 0 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 2
Mitochondria 13 1 0 2 6 21 67 63 145 113 102 72 2 0 1 6 1 3 1 1 0 1 1 10
Nucleus 222 96 93 88 250 186 276 243 250 259 178 202 176 183 219 204 103 176 216 107 102 141 216 165
Nuclear Periphery 3 1 0 0 3 2 6 1 4 1 4 2 1 0 7 0 2 0 0 0 1 0 0 3
Nucleolus 1 1 3 7 3 4 10 13 19 21 15 9 3 2 12 3 3 9 2 0 0 2 17 18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 1 3 1 1 3 1 1 5 1 1 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 3 0 6 6 3 1 2 1 1 0 0 0 8 11 11 13 5 2 3 7 34 40
Unique Cell Count 230 101 100 97 280 212 326 278 304 320 226 241 183 205 267 240 128 208 228 119 111 159 282 251
Labelled Cell Count 239 104 101 104 291 238 388 352 445 421 330 310 188 205 287 248 134 220 228 119 111 159 282 251


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 8.7 7.1 6.4 7.0 5.9 5.3 5.3 4.3 4.9 4.3 4.9 8.0 8.4 7.9 11.8 11.0 10.9 8.6 8.9 8.9
Std Deviation (1e-4) 0.9 1.5 1.7 1.3 2.2 2.3 1.9 1.5 1.1 1.6 1.1 1.4 1.8 2.8 2.6 3.3 2.9 2.7 1.8 1.7 1.5
Intensity Change (Log2) – – – -0.15 -0.01 -0.26 -0.42 -0.43 -0.71 -0.53 -0.71 -0.52 0.18 0.24 0.16 0.74 0.64 0.61 0.28 0.32 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.1 1.7 1.9 1.9 0 0 0 0 0 2.4 2.9 1.9 0 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 4.9 5.2 0 7.0 0 6.2 0 0 0 0 0 0
Nucleus -0.6 -1.1 -1.4 -2.1 -1.5 -2.6 -2.9 -3.2 -2.3 1.2 -1.0 -2.6 -2.0 -2.7 -2.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1.3 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.7 1.2