Standard name
Human Ortholog
Description Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.94 0.99 0.95 0.94 0.82 0.69 0.76 0.66 0.69 0.7 0.98 0.98 0.98 0.93 0.92 0.91 0.96 0.93 0.93 0.96 0.92 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.13 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.1 0.24 0.16 0.25 0.21 0.18 0 0 0 0.07 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 1 1 0 5 0 0 0 0 0 0 1 0 0 0 2 1
Bud 0 0 0 1 1 0 1 1 1 1 4 4 1 1 0 0 0 0 0 0 0 1 5 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 7 11 10 3 16 6 3 1 3 4 4 6 7 0 0 0 0 0 0
Cytoplasm 297 17 34 205 237 259 384 281 231 245 159 191 473 640 716 191 227 213 291 28 58 272 492 529
Endoplasmic Reticulum 1 0 0 2 3 3 21 14 8 16 2 8 0 3 5 5 5 4 0 0 1 0 5 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 2 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 1
Mitochondria 3 0 1 0 0 3 17 3 16 15 30 16 0 1 1 1 0 1 2 0 0 0 1 2
Nucleus 0 0 1 3 3 9 11 14 9 8 5 12 12 2 4 0 1 1 0 1 0 3 8 4
Nuclear Periphery 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 2 18 11 47 98 50 92 49 49 10 9 16 14 21 20 1 0 0 4 11 12
Unique Cell Count 299 17 36 207 249 277 471 410 304 374 231 273 485 650 729 205 246 233 305 31 63 284 536 568
Labelled Cell Count 301 17 36 215 263 293 495 421 319 394 255 288 497 660 746 219 263 246 305 31 63 284 536 568


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 12.4 9.8 11.0 13.3 12.6 12.2 13.4 11.9 12.9 11.5 12.0 14.4 19.7 21.1 35.5 36.0 33.0 11.0 12.6 15.8
Std Deviation (1e-4) 2.2 4.7 1.8 2.0 3.7 3.3 3.0 3.6 3.6 4.0 5.4 3.9 3.6 5.6 6.2 16.5 17.1 14.0 2.3 3.0 3.6
Intensity Change (Log2) 0.17 0.43 0.36 0.31 0.45 0.28 0.39 0.22 0.29 0.55 1.0 1.1 1.85 1.87 1.75 0.16 0.35 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 0.2 -0.2 -2.0 -3.3 -2.5 -3.6 -3.2 -3.1 1.1 1.8 1.6 -0.3 -0.5 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 2.6 3.4 3.1 2.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.014 14.1034 13.0919 11.3324 9.9462 12.4758 6.913 10.4144 9.1402 8.0994 7.5885 8.7611 12.6571 12.6962 12.0036 12.7549 10.1634 12.4853
Actin 0.0002 0.0004 0.0018 0.0002 0.0004 0.0003 0.0322 0.0002 0.0063 0.0134 0.0438 0.0001 0.012 0.0003 0.0005 0.0002 0.0413 0.0004
Bud 0.0002 0.0004 0.0028 0.0002 0.002 0.0004 0.001 0.0001 0.0004 0.0002 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001 0.0012 0.0001
Bud Neck 0.0003 0.0003 0.0005 0.0007 0.0014 0.0011 0.0012 0.0001 0.0012 0.0009 0.0015 0.0008 0.0016 0.0001 0.0003 0.0003 0.003 0.0007
Bud Periphery 0 0.0001 0.0009 0 0.0008 0.0002 0.0009 0 0.0003 0.0001 0.0002 0 0.0004 0 0 0 0.002 0
Bud Site 0.0002 0.0008 0.0007 0.0001 0.0003 0.0001 0.0011 0.0001 0.0017 0.0004 0.0004 0 0.0011 0.0001 0.0002 0.0001 0.0038 0
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.001 0
Cytoplasm 0.9396 0.957 0.9386 0.9057 0.9324 0.9333 0.818 0.9891 0.9191 0.9264 0.8639 0.9614 0.9146 0.979 0.9593 0.9212 0.8132 0.9464
Cytoplasmic Foci 0.0037 0.0022 0.003 0.0024 0.0031 0.0027 0.0358 0.001 0.0086 0.0066 0.0072 0.0041 0.0131 0.0015 0.0054 0.0053 0.0089 0.0021
Eisosomes 0 0 0 0 0 0 0.0006 0 0 0.0001 0.0003 0 0.0002 0 0 0 0.0004 0
Endoplasmic Reticulum 0.002 0.0015 0.0015 0.0021 0.003 0.0024 0.0029 0.0006 0.0013 0.0021 0.002 0.0014 0.0024 0.0003 0.001 0.0012 0.0083 0.0037
Endosome 0.0014 0.0014 0.0009 0.0017 0.0019 0.0022 0.0236 0.0002 0.0107 0.0138 0.0124 0.0027 0.0069 0.0001 0.0059 0.0343 0.021 0.0051
Golgi 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0071 0 0.0032 0.0059 0.0052 0 0.0019 0.0001 0.0013 0.0011 0.0093 0.0001
Lipid Particles 0 0.0001 0 0 0 0 0.0113 0 0.0066 0.0058 0.0046 0 0.0026 0.0005 0.0014 0.0012 0.0334 0.0001
Mitochondria 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0048 0.0001 0.0081 0.0013 0.0009 0.0001 0.0027 0.0001 0.0003 0.0002 0.0096 0.0002
None 0.0029 0.0017 0.0025 0.0015 0.0026 0.0016 0.0144 0.0016 0.0019 0.0021 0.0042 0.0016 0.0029 0.0008 0.0047 0.0007 0.0033 0.0009
Nuclear Periphery 0.0049 0.0038 0.0056 0.006 0.0048 0.0052 0.006 0.0007 0.0068 0.0027 0.0066 0.0021 0.0053 0.0008 0.002 0.0029 0.0086 0.009
Nucleolus 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0009 0 0.0002 0.0001 0.0007 0 0.0001 0 0.0003 0 0.0005 0.0001
Nucleus 0.042 0.0278 0.0345 0.0763 0.0433 0.0469 0.0151 0.0058 0.0145 0.0121 0.016 0.0239 0.0258 0.0053 0.0132 0.0259 0.0138 0.0279
Peroxisomes 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0129 0 0.0053 0.003 0.0021 0 0.0016 0.0105 0.0006 0.0001 0.0092 0
Punctate Nuclear 0.001 0.0011 0.0048 0.0015 0.0016 0.0019 0.0065 0.0002 0.0008 0.0022 0.0265 0.0008 0.0015 0.0003 0.0024 0.0005 0.0045 0.0017
Vacuole 0.0011 0.0008 0.001 0.001 0.0016 0.0011 0.0026 0.0002 0.0023 0.0005 0.0008 0.0006 0.0021 0.0001 0.0008 0.0036 0.0028 0.001
Vacuole Periphery 0.0002 0.0001 0.0002 0.0002 0.0002 0.0003 0.001 0 0.0006 0.0003 0.0004 0.0002 0.0007 0 0.0002 0.0009 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.8831 22.0531 31.9849 55.5094 39.9815 58.2777 50.9304 66.0129 85.3421 64.7434
Translational Efficiency 1.8706 2.1126 1.5316 1.4509 1.8815 1.8592 1.3918 1.4331 1.4125 1.4772

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency