Standard name
Human Ortholog
Description Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.1 – 0.08 0.08 0.06 0.05 – 0.07 0 0 0.08 – 0.12 0.06 0.17 0.16 0.15 0.06 0.05 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0 0 – 0 0.05 0 0 – 0 0.1 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – 0.08 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0.05 – 0.05 0.05 0 0.06 – 0 0 0 0 0 0.05 0.09 0.09 0.08 0.09 0.06
Golgi 0.29 0.59 – 0.55 0.75 0.31 0.28 – 0.1 0.15 0 0 – 0.61 0.77 0.84 0.8 0.83 0.74 0.65 0.55 0.82 0.7 0.81
Mitochondria 0.85 0.65 – 0.78 0.42 0.73 0.63 – 0.76 0.67 0.84 0.73 – 0.43 0.22 0.12 0.06 0.08 0 0.06 0.09 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.06 – 0.06 0.07 0.11 0.17 – 0.14 0.14 0.21 0.13 – 0.16 0 0.06 0.12 0.1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0.06 – 0 0 0 0 0 0.05 0.05 0.06 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 – 0 0.06 0 0 – 0.05 0 0 0 – 0.06 0 0.08 0.07 0.09 0 0.06 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 15 – 9 16 12 15 – 12 8 5 16 – 6 7 55 61 53 14 9 0 0 1 5
Bud 1 0 – 1 0 2 5 – 4 10 4 8 – 0 0 2 3 2 0 0 1 0 1 1
Bud Neck 0 0 – 0 0 0 1 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 2 3 4 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 1 0 – 1 1 1 2 – 0 0 3 3 1 0 0 0 0 0 0
Cytoplasm 0 4 – 3 8 3 5 – 7 10 6 4 – 1 11 13 6 14 0 1 0 0 0 0
Endoplasmic Reticulum 1 2 – 0 1 0 0 – 0 2 0 0 – 4 2 9 4 10 0 0 0 0 0 0
Endosome 5 1 – 2 5 5 14 – 9 10 4 11 – 2 5 2 2 4 12 15 2 1 6 8
Golgi 64 92 – 62 151 59 77 – 18 32 6 7 – 30 88 278 300 286 175 110 16 15 47 112
Mitochondria 189 100 – 87 85 137 173 – 133 142 139 145 – 21 25 38 22 29 9 10 2 0 2 3
Nucleus 0 0 – 0 0 0 3 – 1 0 2 1 – 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 3 0 – 0 0 0 5 – 2 2 2 2 – 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 35 10 – 7 15 20 48 – 24 30 34 26 – 8 2 21 45 34 2 2 0 0 0 0
Peroxisomes 3 0 – 2 5 6 9 – 4 7 6 12 – 0 1 2 0 0 12 8 1 0 0 1
SpindlePole 0 0 – 0 1 2 4 – 2 3 3 11 – 0 3 1 1 3 4 0 0 0 0 0
Vac/Vac Membrane 9 17 – 5 13 5 7 – 8 8 2 5 – 3 5 26 25 30 4 9 1 0 2 5
Unique Cell Count 223 155 112 202 188 276 175 212 165 198 49 115 330 373 345 238 170 30 19 68 140
Labelled Cell Count 328 241 178 300 252 366 225 267 217 254 75 149 450 472 467 238 170 30 19 68 140


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.9 26.2 – 18.7 19.9 17.1 16.2 – 12.9 13.3 11.0 11.8 – 18.9 18.1 28.9 27.5 27.6 – 15.1 18.9
Std Deviation (1e-4) 3.0 4.8 – 3.4 3.6 2.9 3.1 – 2.8 3.0 2.5 2.7 – 5.2 4.3 6.8 5.9 6.4 – 3.5 4.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.7074 15.8974 14.1408 13.4043 10.7836 14.2941 12.9636 17.1359 15.5737 15.4456 12.7423 16.3461 11.2408 14.9865 13.772 13.3901 8.1585 14.3233
Actin 0.0287 0.0159 0.0225 0.018 0.0768 0.0051 0.0268 0.0214 0.0177 0.0031 0.0044 0.019 0.0315 0.0194 0.0354 0.0211 0.1135 0.004
Bud 0.0004 0.0005 0.0003 0.0017 0.0005 0.0002 0.0006 0.0009 0.0002 0.0002 0.0005 0.0001 0.0008 0.0013 0.0004 0.0006 0.0004 0.0001
Bud Neck 0.0011 0.0006 0.0004 0.0007 0.0012 0.0003 0.0009 0.0006 0.0004 0.0002 0.0005 0.0005 0.012 0.0005 0.0035 0.0004 0.0218 0.0003
Bud Periphery 0.0005 0.0003 0.0003 0.0021 0.0004 0.0002 0.0008 0.0007 0.0002 0.0003 0.0008 0.0002 0.0009 0.0013 0.0003 0.0005 0.0004 0.0001
Bud Site 0.0037 0.003 0.0008 0.0046 0.0062 0.0001 0.0037 0.004 0.0014 0.0002 0.0014 0.0002 0.0084 0.0055 0.0054 0.0021 0.002 0.0001
Cell Periphery 0.0002 0.0004 0.0001 0.0002 0.0002 0.0001 0.0005 0.0002 0.0002 0.0001 0.0003 0.0002 0.0003 0.0003 0.0002 0.0002 0.0002 0.0001
Cytoplasm 0.0015 0.0014 0.0014 0.0006 0.0068 0.0033 0.0019 0.0011 0.0013 0.0006 0.0022 0.0003 0.0034 0.0018 0.0017 0.0015 0.0009 0.0002
Cytoplasmic Foci 0.0581 0.0793 0.0537 0.0391 0.0846 0.0278 0.0445 0.0601 0.038 0.0435 0.0497 0.0176 0.0534 0.0489 0.0419 0.0381 0.0728 0.0157
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0004 0.0001 0.0003 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0016 0.0003 0.0007 0.0004 0.0033 0.0003 0.0021 0.0003 0.0007 0.0015 0.0011 0.0004 0.002 0.0002 0.0008 0.0027 0.0005 0.0003
Endosome 0.1534 0.1783 0.1481 0.2357 0.1644 0.1 0.1855 0.1697 0.1465 0.2251 0.151 0.0804 0.1273 0.1572 0.1382 0.1719 0.1913 0.0822
Golgi 0.5766 0.6144 0.6546 0.5966 0.5485 0.7984 0.5642 0.6179 0.6518 0.6292 0.5391 0.7372 0.5854 0.6713 0.6697 0.6557 0.3972 0.797
Lipid Particles 0.0368 0.0352 0.0243 0.0427 0.029 0.0125 0.0529 0.0332 0.0381 0.0311 0.079 0.0512 0.0383 0.031 0.0305 0.0356 0.0663 0.0201
Mitochondria 0.1041 0.0396 0.0649 0.0395 0.0431 0.0423 0.0699 0.0566 0.0568 0.0454 0.1275 0.0572 0.0885 0.0318 0.0475 0.0228 0.0027 0.0575
None 0.0009 0 0.0004 0.0001 0.0026 0.002 0.0026 0.0001 0.0029 0.0003 0.0066 0.0001 0.01 0.0001 0.0017 0.0011 0.0004 0
Nuclear Periphery 0.0022 0.0001 0.0048 0.0002 0.002 0.0002 0.0051 0.0001 0.0008 0.0024 0.0027 0.0015 0.0048 0.0001 0.0012 0.0031 0.0003 0.0001
Nucleolus 0.0003 0.004 0.0003 0.0002 0.0005 0.0004 0.0047 0.0002 0.0082 0.0002 0.0006 0.0006 0.0013 0.0003 0.0004 0.0008 0.0004 0.0001
Nucleus 0.0004 0.0005 0.0018 0.0001 0.001 0.0007 0.0013 0.0001 0.0005 0.0003 0.0005 0.0002 0.0021 0.0002 0.0004 0.0009 0.0002 0
Peroxisomes 0.0152 0.0146 0.0083 0.0068 0.0147 0.0019 0.0122 0.0181 0.0142 0.0041 0.0075 0.0034 0.0125 0.0046 0.0108 0.0071 0.1141 0.0159
Punctate Nuclear 0.0015 0.0002 0.0018 0.0004 0.0045 0.0001 0.0013 0.0002 0.0011 0.0006 0.0008 0.0003 0.0062 0.0001 0.001 0.0013 0.004 0
Vacuole 0.0066 0.0068 0.0044 0.0035 0.005 0.0013 0.0075 0.0047 0.0065 0.0045 0.0091 0.0077 0.0041 0.0139 0.0039 0.0158 0.0033 0.0017
Vacuole Periphery 0.0058 0.0046 0.0061 0.0069 0.0045 0.0027 0.0108 0.0095 0.0124 0.0071 0.0146 0.0216 0.0063 0.0103 0.0049 0.0163 0.0071 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 95.4024 103.7995 76.191 85.6261 98.4665 86.5674 106.228 99.3608 82.2453 97.1368
Translational Efficiency 1.2341 1.1785 1.1544 0.9349 1.125 1.6379 1.1781 1.2402 1.3428 1.3312

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1888 1099 193 1090 1405 1119 1622 869 3293 2218 1815 1959

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1245.76 1439.87 2459.69 1922.89 1511.68 1481.28 2063.55 1924.19 1359.22 1460.76 2105.67 1923.47
Standard Deviation 201.89 307.18 321.02 340.07 259.84 248.24 307.44 327.72 263.58 279.77 332.17 334.65
Intensity Change Log 2 — 0.208912 0.981450 0.626250 — -0.029308 0.448976 0.348098 — 0.083207 0.714027 0.480434

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.014142 0.028887 0.134080* 0.027110 0.011660 0.005848 0.025273 0.015573 0.013083 0.017264 0.036843 0.021992
Bud Neck 0.001711 0.002459 0.021703 0.002122 0.001272 0.001770 0.003825 0.002674 0.001524 0.002111 0.005726 0.002367
Bud Site 0.001897 0.002774 0.007998 0.024483 0.001488 0.002777 0.002611 0.011935 0.001722 0.002775 0.003184 0.018917
Cell Periphery 0.000192 0.000270 0.000536 0.001206 0.000168 0.000150 0.000222 0.000435 0.000182 0.000210 0.000256 0.000864
Cytoplasm 0.001022 0.001096 0.000580 0.005629 0.000686 0.001279 0.000853 0.002130 0.000878 0.001188 0.000824 0.004077
Cytoplasmic Foci 0.084511 0.120314 0.079420 0.048214 0.093490 0.150777 0.083144 0.077527 0.088342 0.135683 0.082748 0.061217
Eisosomes 0.000373 0.000474 0.001664 0.000226 0.000375 0.000128 0.000303 0.000136 0.000374 0.000299 0.000448 0.000186
Endoplasmic Reticulum 0.000620 0.000907 0.003202 0.001862 0.000764 0.000444 0.000673 0.000905 0.000682 0.000674 0.000942 0.001437
Endosome 0.037197 0.066045 0.023226 0.068248 0.026946 0.093148 0.041639 0.103339 0.032823 0.079718 0.039681 0.083814
Golgi 0.322364* 0.515004* 0.369386* 0.710818* 0.430472* 0.409689* 0.562845* 0.615668* 0.368490* 0.461872* 0.542274* 0.668610*
Lipid Particles 0.024890 0.045533 0.183493* 0.011470 0.030944 0.036263 0.075025 0.027170 0.027473 0.040856 0.086559 0.018434
Mitochondria 0.477744* 0.174018* 0.067462 0.025147 0.367979* 0.242434* 0.114973* 0.025033 0.430911* 0.208535* 0.109921* 0.025096
Mitotic Spindle 0.001266 0.001263 0.016924 0.005658 0.001256 0.005274 0.009389 0.012954 0.001262 0.003286 0.010190 0.008894
None 0.000721 0.000492 0.001202 0.000209 0.000799 0.000301 0.000598 0.000188 0.000754 0.000396 0.000662 0.000199
Nuclear Periphery 0.000147 0.000325 0.010149 0.000212 0.000428 0.000527 0.002522 0.001263 0.000267 0.000427 0.003333 0.000679
Nuclear Periphery Foci 0.000359 0.001244 0.004998 0.000785 0.000594 0.001176 0.001877 0.002090 0.000459 0.001210 0.002208 0.001364
Nucleolus 0.000521 0.001506 0.000521 0.000082 0.000285 0.000252 0.000308 0.000341 0.000420 0.000873 0.000330 0.000197
Nucleus 0.000096 0.000245 0.000219 0.000065 0.000185 0.000077 0.000076 0.000122 0.000134 0.000160 0.000091 0.000090
Peroxisomes 0.014568 0.013469 0.006062 0.002857 0.005453 0.010752 0.002744 0.002152 0.010679 0.012099 0.003097 0.002544
Vacuole 0.006819 0.011542 0.034681 0.040578 0.009189 0.018798 0.030083 0.063964 0.007830 0.015203 0.030572 0.050952
Vacuole Periphery 0.008838 0.012131 0.032495 0.023020 0.015568 0.018137 0.041017 0.034401 0.011709 0.015161 0.040110 0.028068

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.62 -7.73 -4.29 0.45 6.82 3.89 -5.47 -1.40 -3.61 2.90 -2.34 -8.37 -4.25 -1.97 4.55
Bud Neck -1.99 -7.73 -1.55 0.55 7.53 -2.09 -7.90 -4.72 -2.99 2.28 -2.50 -9.60 -3.98 -1.82 7.25
Bud Site -1.80 -3.83 -9.55 -9.13 -5.88 -2.27 -2.73 -7.31 -6.37 -6.65 -2.85 -4.37 -11.75 -10.96 -10.69
Cell Periphery -2.69 -5.65 -5.60 -5.14 -3.52 1.35 -4.25 -6.93 -7.50 -5.60 -1.75 -5.80 -6.64 -6.35 -5.92
Cytoplasm -0.78 4.26 -5.94 -5.86 -6.46 -4.18 -1.42 -3.88 -2.51 -3.41 -3.54 0.40 -6.98 -6.32 -6.95
Cytoplasmic Foci -8.52 0.59 9.77 15.88 3.59 -11.67 2.83 3.24 12.89 1.11 -14.62 1.89 8.99 20.56 6.34
Eisosomes -2.23 -7.67 4.33 5.05 8.50 9.75 2.44 8.41 -0.54 7.61 2.85 -2.50 8.25 4.26 8.71
Endoplasmic Reticulum -3.96 -6.06 -3.49 -2.66 2.44 3.32 0.97 -1.06 -3.74 -1.90 0.12 -3.21 -3.66 -3.69 -2.30
Endosome -9.67 5.58 -10.85 -0.63 -14.39 -18.04 -7.35 -17.48 -1.87 -13.75 -19.50 -3.93 -20.03 -1.28 -16.10
Golgi -17.62 -2.24 -35.75* -15.92 -15.99 1.79 -12.01 -13.65 -14.99 -3.85 -11.54 -19.67 -34.80 -22.34 -12.63
Lipid Particles -6.57 -10.70 6.22 11.00 11.59 -1.47 -10.84 1.06 2.21 10.59 -5.63 -15.63 4.46 8.84 17.57
Mitochondria 25.93* 32.45* 50.09* 17.74 4.40 9.40 23.36 34.25* 21.87 14.68* 24.84* 39.96* 59.85* 28.03 16.26*
Mitotic Spindle 0.03 -3.12 -5.41 -5.71 2.16 -5.10 -5.63 -5.24 -3.41 -1.54 -4.56 -6.38 -6.99 -5.07 0.47
None 6.53 -4.60 16.86 10.12 9.70 16.15 5.53 19.46 5.49 14.62 15.29 3.00 25.27 11.21 17.48
Nuclear Periphery -6.56 -3.82 -2.90 3.07 3.80 -1.29 -7.97 -3.04 -2.70 3.37 -4.19 -8.40 -3.37 -2.07 6.90
Nuclear Periphery Foci -2.99 -4.14 -4.34 1.33 3.70 -2.96 -6.23 -4.80 -2.66 -0.63 -4.26 -8.28 -6.14 -0.83 3.21
Nucleolus -1.39 -0.02 2.11 2.07 4.31 0.39 -0.28 -0.80 -0.95 -0.73 -1.26 0.67 0.82 1.65 0.43
Nucleus -2.11 -4.64 1.50 2.50 5.96 0.95 0.96 0.18 -1.13 -1.14 -0.44 0.85 0.40 1.03 -0.55
Peroxisomes 0.57 5.14 9.40 6.90 2.65 -3.77 4.20 5.14 6.57 1.56 -1.17 9.77 10.59 9.44 1.52
Vacuole -3.43 -6.59 -12.41 -10.19 -1.45 -5.91 -11.51 -14.92 -11.89 -8.75 -6.93 -14.32 -19.27 -15.33 -8.02
Vacuole Periphery -3.52 -6.42 -9.32 -6.75 2.41 -1.80 -13.34 -9.17 -7.72 2.72 -4.13 -17.63 -13.11 -9.83 6.31
Standard name
Human Ortholog
Description Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (11%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gga2

Gga2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gga2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available