Standard name
Description Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.1 0.08 0.08 0.06 0.05 0.07 0 0 0.08 0.12 0.06 0.17 0.16 0.15 0.06 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 0 0 0.1 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.05 0.05 0.05 0 0.06 0 0 0 0 0 0.05 0.09 0.09 0.08 0.09 0.06
Golgi 0.29 0.59 0.55 0.75 0.31 0.28 0.1 0.15 0 0 0.61 0.77 0.84 0.8 0.83 0.74 0.65 0.55 0.82 0.7 0.81
Mitochondria 0.85 0.65 0.78 0.42 0.73 0.63 0.76 0.67 0.84 0.73 0.43 0.22 0.12 0.06 0.08 0 0.06 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.06 0.06 0.07 0.11 0.17 0.14 0.14 0.21 0.13 0.16 0 0.06 0.12 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05 0.05 0.06 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0.06 0 0 0.05 0 0 0 0.06 0 0.08 0.07 0.09 0 0.06 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 15 9 16 12 15 12 8 5 16 6 7 55 61 53 14 9 0 0 1 5
Bud 1 0 1 0 2 5 4 10 4 8 0 0 2 3 2 0 0 1 0 1 1
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 3 4 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 1 1 1 2 0 0 3 3 1 0 0 0 0 0 0
Cytoplasm 0 4 3 8 3 5 7 10 6 4 1 11 13 6 14 0 1 0 0 0 0
Endoplasmic Reticulum 1 2 0 1 0 0 0 2 0 0 4 2 9 4 10 0 0 0 0 0 0
Endosome 5 1 2 5 5 14 9 10 4 11 2 5 2 2 4 12 15 2 1 6 8
Golgi 64 92 62 151 59 77 18 32 6 7 30 88 278 300 286 175 110 16 15 47 112
Mitochondria 189 100 87 85 137 173 133 142 139 145 21 25 38 22 29 9 10 2 0 2 3
Nucleus 0 0 0 0 0 3 1 0 2 1 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 3 0 0 0 0 5 2 2 2 2 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 35 10 7 15 20 48 24 30 34 26 8 2 21 45 34 2 2 0 0 0 0
Peroxisomes 3 0 2 5 6 9 4 7 6 12 0 1 2 0 0 12 8 1 0 0 1
SpindlePole 0 0 0 1 2 4 2 3 3 11 0 3 1 1 3 4 0 0 0 0 0
Vac/Vac Membrane 9 17 5 13 5 7 8 8 2 5 3 5 26 25 30 4 9 1 0 2 5
Unique Cell Count 223 155 112 202 188 276 175 212 165 198 49 115 330 373 345 238 170 30 19 68 140
Labelled Cell Count 328 241 178 300 252 366 225 267 217 254 75 149 450 472 467 238 170 30 19 68 140


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.9 26.2 18.7 19.9 17.1 16.2 12.9 13.3 11.0 11.8 18.9 18.1 28.9 27.5 27.6 15.1 18.9
Std Deviation (1e-4) 3.0 4.8 3.4 3.6 2.9 3.1 2.8 3.0 2.5 2.7 5.2 4.3 6.8 5.9 6.4 3.5 4.2
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole