Standard name
Description SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.75 0.48 0.49 0.54 0.48 0.56 0.44 0.43 0.4 0.4 0.42 0.41 0.45 0.47 0.48 0.55 0.46 0.79 0.71 0.63 0.55 0.56 0.5
Bud 0.12 0.15 0.14 0.21 0.2 0.16 0.15 0.18 0.13 0.13 0.16 0.07 0.14 0.12 0.1 0.09 0.09 0.05 0 0.07 0.13 0.1 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.11 0.09 0.09
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.12 0.2 0.09 0.08 0.07 0.09 0.09 0.08 0.11 0.09 0.12 0.15 0.25 0.15 0.14 0.12 0 0 0 0 0 0
Cytoplasm 0.06 0.34 0.36 0.27 0.31 0.26 0.27 0.21 0.24 0.17 0.17 0.51 0.41 0.36 0.31 0.23 0.33 0 0.1 0.07 0.08 0.11 0.2
Endoplasmic Reticulum 0.06 0.17 0.1 0.17 0.09 0.2 0.19 0.16 0.2 0.22 0.15 0.06 0.1 0.09 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.15 0.12 0 0 0 0 0 0
Mitochondria 0.19 0 0 0 0 0.05 0.08 0.2 0.17 0.44 0.27 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0.07 0.05 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0.06 0 0.05 0 0 0.05 0.06 0 0.07 0.1 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 61 34 86 98 111 131 121 111 89 118 33 86 103 150 186 98 61 105 81 41 130 133
Bud 2 19 10 34 41 32 44 50 36 29 44 6 26 26 32 32 19 3 5 8 10 23 25
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 7 7 8 22 23
Bud Site 0 2 0 2 2 5 1 7 4 8 6 0 2 1 8 7 3
Cell Periphery 0 16 14 15 17 15 28 24 22 25 24 10 29 55 48 49 26 0 3 1 0 2 4
Cytoplasm 1 44 25 43 64 52 81 60 66 38 48 41 78 78 98 79 69 3 14 8 5 26 52
Endoplasmic Reticulum 1 22 7 27 18 41 57 46 54 49 42 5 19 19 16 19 12 0 1 3 1 1 3
Endosome 0 0 0 0 12 2 15 10 11 2 11 1 1 0 3 2 5 3 3 6 1 8 6
Golgi 0 3 2 2 7 1 2 4 5 2 6 1 1 4 41 50 26 0 1 1 0 1 0
Mitochondria 3 0 0 1 0 10 25 56 48 98 76 0 0 1 16 8 2 2 1 4 1 9 5
Nucleus 0 1 0 1 0 1 0 1 2 1 1 0 0 1 2 0 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 2 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 2 1 0 1 2 0 0 0 0 3 1 0 0 0 0 0 0
Peroxisomes 0 3 2 3 11 8 20 14 10 4 18 3 9 8 7 7 7 0 1 2 1 0 0
SpindlePole 0 2 1 3 12 1 18 9 14 1 9 4 12 8 22 34 17 0 0 0 0 1 1
Vac/Vac Membrane 0 0 2 2 13 0 5 6 9 1 6 1 1 0 12 15 12 0 0 0 0 2 1
Unique Cell Count 16 128 69 160 205 200 301 280 276 223 282 81 192 218 313 340 212 78 148 129 76 235 267
Labelled Cell Count 19 173 97 220 296 279 430 411 393 348 411 105 264 306 455 494 303 78 148 129 76 235 267


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.9 9.6 9.2 9.6 8.2 8.2 7.3 7.5 7.0 7.4 9.7 9.7 9.9 12.9 13.4 12.8 8.7 9.0 9.2
Std Deviation (1e-4) 1.1 1.5 1.6 1.5 1.7 1.5 1.5 1.5 1.6 1.4 1.7 1.4 1.4 1.5 2.5 2.4 2.4 1.2 1.4 1.4
Intensity Change (Log2) -0.08 -0.01 -0.24 -0.23 -0.41 -0.35 -0.47 -0.39 0.01 0.01 0.04 0.42 0.47 0.41 -0.15 -0.1 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -0.2 0.9 -0.9 -0.9 -1.4 -1.4 -1.1 -1.0 -0.6 -0.3 -0.2 0.8 -0.4
Bud 1.2 1.0 0.3 0 0.7 -0.3 -0.3 0.2 -1.4 -0.2 -0.6 -1.0 -1.3 -1.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.3 -2.7 -3.0 -2.6 -2.8 -3.0 -1.9 -2.8 -1.3 -1.0 0.8 -1.0 -1.2 -1.7
Cytoplasm -1.4 -0.8 -1.6 -1.5 -2.6 -2.1 -3.4 -3.5 1.8 0.6 -0.1 -0.8 -2.3 -0.6
Endoplasmic Reticulum 1.3 -0.3 1.9 1.7 1.3 1.8 2.2 1.0 -0.9 -0.1 -0.4 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 2.4 2.7 2.3
Mitochondria 0 0 0 0 4.1 3.7 6.8 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bzz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available