Standard name
Human Ortholog
Description Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation and maturation of a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.97 0.98 0.88 0.87 0.8 0.76 0.7 0.74 0.99 1.0 1.0 0.91 0.89 0.89 0.96 0.95 0.96 0.93 0.89 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.1 0.24 0.25 0.34 0.27 0 0 0 0.08 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0.13 0.13 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 3 2 0 0 0 0 0 0 1 0 0 0 1 3
Bud 1 0 0 0 1 2 0 3 4 3 5 0 0 0 0 0 0 0 1 0 1 0 3
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 6 5 7 3 8 0 8 6 13 11 13 9 8 11 4 6 8 0 0 0 0 0 0
Cytoplasm 228 162 172 130 191 194 294 218 242 166 246 317 295 351 139 106 87 220 155 171 185 174 261
Endoplasmic Reticulum 1 0 0 0 1 2 1 2 2 3 6 1 1 1 8 6 3 1 0 0 3 5 4
Endosome 1 0 0 0 0 0 1 1 3 2 0 0 0 0 1 1 0 0 0 0 1 0 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 1 0
Mitochondria 0 0 0 2 0 45 33 64 81 80 91 0 0 0 12 3 2 0 1 0 0 2 1
Nucleus 0 0 0 0 2 5 4 6 2 5 4 3 0 1 6 3 3 1 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 2 2 7 23 12 18 7 9 0 1 0 20 15 11 0 0 2 3 4 1
Unique Cell Count 230 162 173 134 194 221 337 272 320 238 333 320 296 352 153 119 98 231 165 178 200 196 283
Labelled Cell Count 238 167 181 137 205 255 367 314 368 282 378 330 305 364 190 142 117 231 165 178 200 196 283


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 9.3 8.5 8.3 8.4 5.6 6.9 6.1 6.2 6.1 6.1 9.2 9.3 9.5 9.9 9.8 10.5 8.3 8.7 8.3
Std Deviation (1e-4) 1.0 1.3 1.0 1.4 1.2 1.7 1.9 1.9 1.3 1.7 1.3 1.3 1.3 1.4 2.0 1.8 2.2 1.4 1.8 1.7
Intensity Change (Log2) -0.02 -0.01 -0.59 -0.29 -0.47 -0.45 -0.47 -0.46 0.12 0.14 0.17 0.23 0.21 0.31 -0.02 0.04 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 -1.1 -1.1 0 0.3 -0.1 -0.7 -0.8 -0.5 -0.7 0.4 1.4
Cytoplasm -1.7 -0.9 -4.5 -4.6 -6.0 -6.9 -7.8 -7.2 -0.4 0.4 0.5 -3.7 -4.1 -4.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.3 4.3 6.9 7.2 8.5 7.6 0 0 0 3.8 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.8 1.9 2.4 0 0 0 0 0 4.3 4.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.605 4.1803 3.4702 2.9009 2.9571 3.384 5.4151 6.6103 6.3232 5.6887 6.2302 6.0703 6.9002 8.7426 8.0209 7.5081 7.7701 7.973
Actin 0.0096 0.0016 0.0106 0.0245 0.0112 0.0101 0.0137 0.0022 0.0033 0.018 0.0008 0.0006 0.01 0.0005 0.0032 0.0001 0.0194 0.0005
Bud 0.0004 0.0004 0.0004 0.0006 0.0003 0.002 0.0016 0.0036 0.0027 0.0055 0.0009 0.0007 0.0008 0.0016 0.0006 0.0001 0.0004 0
Bud Neck 0.001 0.0001 0.0004 0.0008 0.0005 0.0011 0.0015 0.0002 0.0003 0.001 0.0003 0.0009 0.0009 0.0001 0.0005 0.0002 0.0004 0.0005
Bud Periphery 0.0004 0.0001 0.0002 0.0005 0.0002 0.0006 0.0039 0.0012 0.001 0.0038 0.0006 0.0002 0.0011 0.0007 0.0003 0 0.0006 0
Bud Site 0.0065 0.0016 0.0007 0.0015 0.0004 0.0002 0.0023 0.0051 0.0029 0.019 0 0.0001 0.0017 0.0042 0.0016 0 0.0007 0
Cell Periphery 0.0004 0.0001 0.0001 0.0001 0.0002 0 0.0015 0.0001 0.0001 0.0002 0 0 0.0002 0.0002 0.0002 0 0.0001 0.0001
Cytoplasm 0.8363 0.9393 0.9198 0.8526 0.8362 0.9277 0.777 0.9464 0.9331 0.8467 0.9791 0.9485 0.9034 0.9643 0.923 0.985 0.8556 0.9763
Cytoplasmic Foci 0.0229 0.0123 0.0118 0.0403 0.0134 0.0084 0.0219 0.0055 0.0124 0.0212 0.0039 0.0092 0.016 0.0018 0.0127 0.0018 0.01 0.0032
Eisosomes 0.0006 0 0.0001 0.0001 0.0004 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0084 0.0015 0.0042 0.0032 0.0145 0.0029 0.0116 0.0024 0.0013 0.002 0.0006 0.0015 0.0085 0.0053 0.003 0.0015 0.0045 0.0038
Endosome 0.0275 0.0083 0.0141 0.0361 0.0393 0.0074 0.0462 0.0014 0.0135 0.0315 0.0016 0.0089 0.0128 0.0009 0.0119 0.0013 0.0234 0.0012
Golgi 0.0057 0.0011 0.0021 0.0098 0.0071 0.0013 0.0222 0.0003 0.0068 0.0041 0.0001 0.0019 0.0041 0.0001 0.0029 0.0001 0.0154 0.0002
Lipid Particles 0.0074 0 0.0009 0.0011 0.0037 0.0008 0.0049 0.0001 0.0003 0.0003 0 0.0001 0.0012 0 0.0012 0 0.0131 0.0001
Mitochondria 0.0047 0.0003 0.0059 0.0026 0.0031 0.0004 0.0199 0.0002 0.0021 0.0024 0.0002 0.0017 0.002 0.0001 0.0014 0 0.0105 0.0001
None 0.0417 0.0301 0.0207 0.0167 0.031 0.0318 0.0087 0.026 0.015 0.0323 0.0097 0.012 0.0202 0.0177 0.0172 0.0082 0.0262 0.0127
Nuclear Periphery 0.0078 0.0004 0.0014 0.0008 0.0287 0.0008 0.0231 0.0005 0.0005 0.0008 0.0003 0.0042 0.0079 0.0004 0.0013 0.0003 0.0061 0.0002
Nucleolus 0.0006 0 0.0002 0 0.0002 0 0.0005 0.0002 0.0001 0.0001 0 0.0001 0.0002 0 0.0052 0 0.0001 0
Nucleus 0.0044 0.0008 0.0014 0.0009 0.0026 0.0016 0.0115 0.0024 0.0012 0.0025 0.0011 0.0025 0.0021 0.0009 0.0035 0.0007 0.0009 0.0005
Peroxisomes 0.0071 0.0005 0.001 0.0045 0.0007 0.0005 0.0072 0.0006 0.0014 0.0013 0.0001 0.0003 0.0022 0 0.0069 0 0.0068 0.0001
Punctate Nuclear 0.0024 0.0002 0.0009 0.0005 0.0021 0.0011 0.0012 0.0009 0.0007 0.0009 0.0002 0.0041 0.002 0.0001 0.0015 0.0001 0.0018 0.0002
Vacuole 0.003 0.0011 0.0026 0.002 0.0018 0.0009 0.0137 0.0006 0.0011 0.0054 0.0004 0.001 0.0015 0.0009 0.0015 0.0005 0.0026 0.0002
Vacuole Periphery 0.0011 0.0002 0.0004 0.0007 0.0024 0.0002 0.0056 0.0001 0.0004 0.0008 0.0001 0.0016 0.0008 0.0001 0.0004 0.0001 0.0013 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.658 46.7678 49.4266 34.0407 28.7767 50.7032 45.4957 40.6197 51.241 42.3954
Translational Efficiency 1.1129 1.4712 1.2552 2.0769 2.0991 1.3871 1.4681 1.5575 1.2321 1.3529

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1825 340 772 2566 1695 2143 1457 1433 3520 2483 2229 3999

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 794.27 1174.55 1061.34 1050.61 859.44 1011.82 1022.80 1033.47 825.65 1034.10 1036.15 1044.47
Standard Deviation 101.59 150.18 123.60 153.46 108.27 134.05 128.14 254.31 109.80 147.40 127.91 195.84
Intensity Change Log 2 0.564407 0.418185 0.403526 0.235484 0.251055 0.266028 0.402831 0.333746 0.333704

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000301 0.000608 0.000286 0.001037 0.000210 0.000303 0.000163 0.002290 0.000257 0.000345 0.000205 0.001486
Bud Neck 0.007340 0.003213 0.001280 0.003475 0.002840 0.002464 0.000813 0.002347 0.005173 0.002566 0.000975 0.003071
Bud Site 0.021120 0.039065 0.013981 0.023399 0.012710 0.014534 0.008292 0.031053 0.017070 0.017893 0.010263 0.026142
Cell Periphery 0.000191 0.000454 0.000108 0.000098 0.000134 0.000065 0.000051 0.000127 0.000163 0.000118 0.000071 0.000108
Cytoplasm 0.584901* 0.739515* 0.781112* 0.805548* 0.697547* 0.792024* 0.826325* 0.759390* 0.639144* 0.784834* 0.810666* 0.789008*
Cytoplasmic Foci 0.261593* 0.144121 0.036852 0.023191 0.185337* 0.103808 0.021781 0.036650 0.224873* 0.109328 0.027001 0.028014
Eisosomes 0.000136 0.000020 0.000018 0.000010 0.000049 0.000019 0.000010 0.000017 0.000094 0.000019 0.000012 0.000013
Endoplasmic Reticulum 0.003489 0.000709 0.001078 0.000284 0.002261 0.000397 0.000393 0.000269 0.002897 0.000440 0.000630 0.000279
Endosome 0.015370 0.006386 0.003139 0.001589 0.009595 0.006329 0.001325 0.005296 0.012589 0.006337 0.001954 0.002917
Golgi 0.015310 0.002146 0.000209 0.000414 0.006729 0.004644 0.000132 0.002649 0.011178 0.004302 0.000158 0.001214
Lipid Particles 0.003938 0.001062 0.000795 0.000356 0.002503 0.000605 0.000330 0.000452 0.003247 0.000667 0.000491 0.000390
Mitochondria 0.003055 0.000487 0.000096 0.000607 0.002761 0.001657 0.000072 0.000642 0.002914 0.001497 0.000080 0.000619
Mitotic Spindle 0.000692 0.000548 0.002866 0.015946 0.002370 0.004922 0.005568 0.026375 0.001500 0.004323 0.004632 0.019683
None 0.001748 0.000456 0.002296 0.002277 0.001839 0.001246 0.005936 0.009993 0.001792 0.001137 0.004675 0.005042
Nuclear Periphery 0.000099 0.000057 0.000268 0.000226 0.000095 0.000042 0.000164 0.000158 0.000097 0.000044 0.000200 0.000201
Nuclear Periphery Foci 0.000496 0.001850 0.005786 0.000892 0.000784 0.000380 0.002479 0.001581 0.000635 0.000581 0.003624 0.001139
Nucleolus 0.000300 0.000085 0.000085 0.000158 0.000189 0.000159 0.000102 0.000361 0.000246 0.000149 0.000096 0.000231
Nucleus 0.017445 0.009376 0.066954 0.046485 0.020513 0.014828 0.063741 0.034496 0.018923 0.014082 0.064854 0.042189
Peroxisomes 0.001824 0.001606 0.000315 0.000671 0.001018 0.002042 0.000260 0.001482 0.001436 0.001982 0.000279 0.000962
Vacuole 0.056212 0.046701 0.082218 0.072251 0.048308 0.048339 0.061986 0.083133 0.052406 0.048115 0.068993 0.076151
Vacuole Periphery 0.004441 0.001536 0.000257 0.001088 0.002206 0.001193 0.000080 0.001238 0.003365 0.001240 0.000141 0.001142

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.55 -1.14 -1.11 -0.22 -0.82 -1.22 -0.23 -1.58 -1.43 -1.54 -1.35 -0.50 -1.94 -1.65 -1.83
Bud Neck 3.72 11.52 7.53 0.45 -3.02 1.26 8.40 3.78 2.47 -4.66 7.28 14.12 7.55 0.70 -4.75
Bud Site -3.97 3.65 3.41 5.27 -1.13 -1.20 4.50 -2.80 -2.09 -5.79 -0.41 7.03 0.43 0.74 -5.46
Cell Periphery -0.83 6.53 7.69 1.21 2.36 9.77 12.03 2.16 -1.35 -1.93 1.29 14.85 6.78 0.66 -1.07
Cytoplasm -10.42 -14.71 -21.80 -0.93 -1.25 -12.38 -7.88 -1.28 9.50 5.69 -23.44 -18.08 -16.29 8.40 3.06
Cytoplasmic Foci 12.42 45.19 50.94 14.48 6.87 15.71 37.02 33.24 21.36 -6.44 27.25 58.96 59.63 28.50 0.24
Eisosomes 7.90 7.89 8.64 3.72 6.35 8.64 11.32 10.25 1.88 -2.73 9.69 10.21 10.60 4.20 3.59
Endoplasmic Reticulum 5.93 5.08 7.48 2.03 7.26 5.67 5.79 6.43 1.89 3.92 8.73 8.03 9.78 2.12 7.82
Endosome 7.41 14.02 17.15 5.23 6.52 4.62 16.89 10.33 4.96 -3.93 9.31 21.30 20.91 8.84 0.25
Golgi 21.01* 25.81 25.64 7.06 -0.84 2.52 10.83 7.85 4.75 -2.76 10.23 25.74 23.62 6.60 -3.05
Lipid Particles 5.90 7.57 8.90 2.02 6.64 7.80 8.66 8.29 1.27 -1.42 10.79 11.29 11.85 2.40 3.52
Mitochondria 6.52 10.72 8.59 0.22 -1.85 1.27 3.53 3.19 2.63 -2.43 2.54 7.21 6.33 2.55 -2.65
Mitotic Spindle 0.14 -2.91 -6.60 -6.96 -4.92 -2.15 -1.70 -4.96 -3.83 -4.05 -3.14 -3.10 -8.00 -5.23 -5.83
None 4.18 -0.83 -1.10 -9.50 -0.23 2.44 -4.52 -2.98 -3.56 -0.35 3.08 -4.54 -3.19 -4.94 1.05
Nuclear Periphery 1.15 -8.30 -5.83 -6.70 1.47 1.56 -5.63 -4.17 -8.94 1.37 2.38 -9.60 -7.03 -11.37 1.06
Nuclear Periphery Foci -5.29 -12.79 -5.54 3.61 11.72 5.84 -10.18 -5.12 -8.42 5.01 0.36 -16.28 -7.17 -7.01 13.25
Nucleolus 5.37 5.53 3.38 -4.00 -5.07 1.80 5.95 -1.73 -2.67 -4.48 4.38 6.96 2.15 -2.71 -6.34
Nucleus 5.96 -16.60 -24.04 -28.14 4.22 5.63 -22.78 -7.31 -10.01 14.91 5.43 -28.32 -24.25 -27.21 12.14
Peroxisomes 1.32 11.75 10.52 6.50 -1.26 -3.06 6.08 0.85 3.17 -2.59 -1.84 12.37 7.46 4.83 -2.91
Vacuole 0.22 -9.69 -19.15 -12.90 -1.90 -1.06 -13.92 -20.70 -20.28 -8.17 0.41 -16.59 -28.43 -27.57 -6.77
Vacuole Periphery 5.00 14.29 10.82 1.48 -1.61 2.82 7.45 4.31 1.79 -1.87 7.34 15.75 10.71 2.39 -2.68