Standard name
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.1 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.93 0.9 0.93 0.91 0.84 0.68 0.73 0.57 0.6 0.54 0.54 0.94 0.98 0.99 0.79 0.85 0.77 0.73 0.75 0.58 0.67 0.81 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.05 0.12 0.09 0.09 0.35 0.47 0.39 0.57 0.59 0.71 0.63 0 0 0 0.06 0.05 0.07 0.11 0.08 0.18 0.11 0.09 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 1 1 1 0 1 1 1 1 0 0 1 0 23 8 11 7 6 7 2 1 3
Bud 2 2 3 0 0 4 1 6 3 6 9 5 2 3 1 15 3 1 1 3 3 3 2 6
Bud Neck 0 0 1 0 0 1 2 1 0 0 0 0 0 0 0 0 1 0 5 6 4 1 3 2
Bud Site 0 0 0 1 0 0 0 0 1 1 0 1 1 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 0 1 0 1 1 4 3 3 2 0 3 3 13 4 5 1 1 0 0 0 2
Cytoplasm 210 266 145 142 155 225 225 251 131 205 102 149 126 311 233 226 99 81 184 232 99 69 147 111
Endoplasmic Reticulum 0 7 3 1 3 1 6 7 6 5 2 2 3 0 0 24 12 5 6 4 6 3 1 3
Endosome 0 1 0 0 0 0 0 2 0 0 0 1 0 3 2 10 2 3 3 4 2 2 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 2
Mitochondria 28 14 19 14 15 95 154 133 131 199 134 174 4 0 2 17 6 7 27 23 30 11 15 13
Nucleus 0 0 0 0 0 1 2 1 1 0 0 0 0 2 1 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 2 0 0 0 1 0 0 0 2 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 1 1 0 0 0 1 0 2 0 1 0 1 0 3 1 0 0 1 0 0 0 0
SpindlePole 1 1 0 2 1 0 3 2 0 3 0 3 0 1 0 9 1 2 0 0 0 1 1 1
Vac/Vac Membrane 0 1 1 0 1 1 6 6 1 7 4 7 0 1 1 7 1 1 10 22 8 2 1 11
Unique Cell Count 224 285 161 152 171 268 329 344 229 339 189 278 134 318 236 286 117 105 253 310 170 104 182 168
Labelled Cell Count 244 295 174 162 177 330 400 412 279 434 257 347 136 326 243 349 139 118 253 310 170 104 182 168


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.3 3.9 3.9 4.1 3.6 3.2 3.7 3.3 3.2 3.4 3.2 5.1 5.1 5.0 5.9 6.9 7.2 4.5 4.5 5.4
Std Deviation (1e-4) 0.5 0.6 1.1 0.9 1.1 0.9 0.7 0.9 0.7 0.7 1.0 0.7 1.1 1.5 1.5 1.3 1.6 1.1 1.1 1.5 1.6
Intensity Change (Log2) – – – -0.03 0.05 -0.14 -0.28 -0.08 -0.27 -0.32 -0.23 -0.3 0.36 0.37 0.34 0.57 0.81 0.86 0.19 0.2 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –