Standard name | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 23 | 8 | 11 | 7 | 6 | 7 | 2 | 1 | 3 |
Bud | 2 | 2 | 3 | 0 | 0 | 4 | 1 | 6 | 3 | 6 | 9 | 5 | 2 | 3 | 1 | 15 | 3 | 1 | 1 | 3 | 3 | 3 | 2 | 6 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 6 | 4 | 1 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 4 | 3 | 3 | 2 | 0 | 3 | 3 | 13 | 4 | 5 | 1 | 1 | 0 | 0 | 0 | 2 |
Cytoplasm | 210 | 266 | 145 | 142 | 155 | 225 | 225 | 251 | 131 | 205 | 102 | 149 | 126 | 311 | 233 | 226 | 99 | 81 | 184 | 232 | 99 | 69 | 147 | 111 |
Endoplasmic Reticulum | 0 | 7 | 3 | 1 | 3 | 1 | 6 | 7 | 6 | 5 | 2 | 2 | 3 | 0 | 0 | 24 | 12 | 5 | 6 | 4 | 6 | 3 | 1 | 3 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 10 | 2 | 3 | 3 | 4 | 2 | 2 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 28 | 14 | 19 | 14 | 15 | 95 | 154 | 133 | 131 | 199 | 134 | 174 | 4 | 0 | 2 | 17 | 6 | 7 | 27 | 23 | 30 | 11 | 15 | 13 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 0 | 2 | 1 | 0 | 3 | 2 | 0 | 3 | 0 | 3 | 0 | 1 | 0 | 9 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 1 | 1 | 6 | 6 | 1 | 7 | 4 | 7 | 0 | 1 | 1 | 7 | 1 | 1 | 10 | 22 | 8 | 2 | 1 | 11 |
Unique Cell Count | 224 | 285 | 161 | 152 | 171 | 268 | 329 | 344 | 229 | 339 | 189 | 278 | 134 | 318 | 236 | 286 | 117 | 105 | 253 | 310 | 170 | 104 | 182 | 168 |
Labelled Cell Count | 244 | 295 | 174 | 162 | 177 | 330 | 400 | 412 | 279 | 434 | 257 | 347 | 136 | 326 | 243 | 349 | 139 | 118 | 253 | 310 | 170 | 104 | 182 | 168 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.3 | 3.9 | 3.9 | 4.1 | 3.6 | 3.2 | 3.7 | 3.3 | 3.2 | 3.4 | 3.2 | 5.1 | 5.1 | 5.0 | 5.9 | 6.9 | 7.2 | 4.5 | 4.5 | 5.4 |
Std Deviation (1e-4) | 0.5 | 0.6 | 1.1 | 0.9 | 1.1 | 0.9 | 0.7 | 0.9 | 0.7 | 0.7 | 1.0 | 0.7 | 1.1 | 1.5 | 1.5 | 1.3 | 1.6 | 1.1 | 1.1 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.05 | -0.14 | -0.28 | -0.08 | -0.27 | -0.32 | -0.23 | -0.3 | 0.36 | 0.37 | 0.34 | 0.57 | 0.81 | 0.86 | 0.19 | 0.2 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3411 | 0.8697 | 0.8132 | 0.5854 | 0.1936 | 0.8163 | 0.549 | 0.742 | 0.6028 | 0.2199 | 0.3007 | 0.5225 | 1.9387 | 1.2812 | 1.8873 | 1.5456 | 1.3015 | 0.9555 |
Actin | 0.0161 | 0.006 | 0.0139 | 0.0637 | 0.0005 | 0.0036 | 0.0471 | 0.0018 | 0.01 | 0.019 | 0.0139 | 0.01 | 0.1306 | 0.1473 | 0.0157 | 0.0009 | 0.0011 | 0.0009 |
Bud | 0.0002 | 0.0085 | 0.003 | 0.0005 | 0.0001 | 0.0011 | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0065 | 0.0002 | 0.0015 | 0.0004 | 0.0004 | 0.0005 | 0.0093 | 0.0001 |
Bud Neck | 0.0115 | 0.002 | 0.0071 | 0.0016 | 0.0005 | 0.0188 | 0.0012 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.0191 | 0.0019 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0033 |
Bud Periphery | 0.0004 | 0.0157 | 0.0038 | 0.0022 | 0.0005 | 0.0014 | 0.0011 | 0.0021 | 0.0004 | 0.0002 | 0.0299 | 0.0006 | 0.0022 | 0.0005 | 0.0007 | 0.0006 | 0.0071 | 0.0001 |
Bud Site | 0.0067 | 0.0406 | 0.0034 | 0.0006 | 0.0003 | 0.0003 | 0.0027 | 0.0058 | 0.0031 | 0.0001 | 0.0089 | 0.0001 | 0.0068 | 0.0067 | 0.0007 | 0.0005 | 0.0004 | 0 |
Cell Periphery | 0.0013 | 0.003 | 0.0007 | 0.0006 | 0.0006 | 0.0003 | 0.0006 | 0.0008 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0014 | 0.0009 | 0.0007 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.1963 | 0.2711 | 0.3475 | 0.2792 | 0.1567 | 0.4025 | 0.1319 | 0.2005 | 0.1964 | 0.3097 | 0.1822 | 0.2508 | 0.0692 | 0.1987 | 0.2682 | 0.3128 | 0.0562 | 0.3151 |
Cytoplasmic Foci | 0.0289 | 0.0401 | 0.0209 | 0.013 | 0.008 | 0.0198 | 0.0381 | 0.0117 | 0.0265 | 0.0439 | 0.0324 | 0.0268 | 0.1059 | 0.0402 | 0.0044 | 0.0418 | 0.0126 | 0.0108 |
Eisosomes | 0.0007 | 0.0006 | 0.0006 | 0.0008 | 0.0004 | 0.0001 | 0.0017 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0018 | 0.001 | 0.0007 | 0.0007 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0042 | 0.0045 | 0.0029 | 0.0065 | 0.0015 | 0.0047 | 0.0074 | 0.0026 | 0.0021 | 0.002 | 0.0011 | 0.0015 | 0.009 | 0.0068 | 0.0169 | 0.0014 | 0.0022 | 0.0022 |
Endosome | 0.0142 | 0.0069 | 0.0064 | 0.0222 | 0.0019 | 0.0076 | 0.0318 | 0.0057 | 0.0185 | 0.0079 | 0.0243 | 0.0096 | 0.1133 | 0.0293 | 0.0113 | 0.0007 | 0.0006 | 0.0061 |
Golgi | 0.0035 | 0.0008 | 0.0011 | 0.0023 | 0.0002 | 0.0004 | 0.0177 | 0.0014 | 0.0043 | 0.001 | 0.0086 | 0.001 | 0.03 | 0.0071 | 0.0131 | 0.0003 | 0.0002 | 0.0002 |
Lipid Particles | 0.0183 | 0.0069 | 0.0013 | 0.0009 | 0.0011 | 0.0018 | 0.0241 | 0.0009 | 0.0031 | 0.0007 | 0.0241 | 0.0033 | 0.0507 | 0.0248 | 0.001 | 0.0013 | 0.0008 | 0.0005 |
Mitochondria | 0.0018 | 0.011 | 0.0022 | 0.0042 | 0.0005 | 0.0004 | 0.0109 | 0.002 | 0.0016 | 0.0007 | 0.007 | 0.0006 | 0.0112 | 0.011 | 0.0255 | 0.0004 | 0.0021 | 0.0008 |
None | 0.6795 | 0.5674 | 0.5729 | 0.5887 | 0.8231 | 0.5291 | 0.6406 | 0.7528 | 0.7215 | 0.6003 | 0.6311 | 0.6642 | 0.3375 | 0.5042 | 0.6353 | 0.6345 | 0.8605 | 0.655 |
Nuclear Periphery | 0.0013 | 0.0015 | 0.0007 | 0.0019 | 0.0004 | 0.0009 | 0.0052 | 0.0006 | 0.0007 | 0.0005 | 0.001 | 0.0003 | 0.0135 | 0.0011 | 0.0018 | 0.0003 | 0.0074 | 0.0007 |
Nucleolus | 0.0006 | 0.0032 | 0.0027 | 0.0006 | 0.0008 | 0.0004 | 0.0022 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0042 | 0.0005 | 0.0001 | 0.0001 | 0.0015 | 0.0001 |
Nucleus | 0.0007 | 0.0019 | 0.0019 | 0.0016 | 0.0007 | 0.0015 | 0.0064 | 0.0009 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.007 | 0.0011 | 0.0006 | 0.0003 | 0.0106 | 0.0005 |
Peroxisomes | 0.0061 | 0.0018 | 0.001 | 0.0006 | 0.0002 | 0.0003 | 0.0144 | 0.0012 | 0.0044 | 0.0102 | 0.0198 | 0.0086 | 0.0329 | 0.0134 | 0.0001 | 0.0009 | 0.0006 | 0.0002 |
Punctate Nuclear | 0.0037 | 0.0017 | 0.0015 | 0.0005 | 0.0006 | 0.0018 | 0.0103 | 0.0004 | 0.0015 | 0.0004 | 0.0016 | 0.0015 | 0.0521 | 0.0017 | 0.0003 | 0.0009 | 0.0237 | 0.0004 |
Vacuole | 0.0036 | 0.0044 | 0.004 | 0.0062 | 0.0012 | 0.0029 | 0.0031 | 0.0063 | 0.003 | 0.0015 | 0.0046 | 0.0008 | 0.0139 | 0.0022 | 0.0014 | 0.0005 | 0.0017 | 0.0022 |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0004 | 0.0014 | 0.0001 | 0.0002 | 0.0011 | 0.0005 | 0.0005 | 0.0003 | 0.0009 | 0.0002 | 0.0033 | 0.0005 | 0.0008 | 0.0001 | 0.0008 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.6531 | 7.1618 | 5.7975 | 9.2588 | 5.6658 | 10.1908 | 9.0516 | 6.8868 | 9.161 | 12.9716 |
Translational Efficiency | 0.6881 | 0.4131 | 0.7132 | 0.7665 | 1.4847 | 1.1001 | 0.4472 | 0.9098 | 0.9223 | 0.7168 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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