Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.1 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.93 0.9 0.93 0.91 0.84 0.68 0.73 0.57 0.6 0.54 0.54 0.94 0.98 0.99 0.79 0.85 0.77 0.73 0.75 0.58 0.67 0.81 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.05 0.12 0.09 0.09 0.35 0.47 0.39 0.57 0.59 0.71 0.63 0 0 0 0.06 0.05 0.07 0.11 0.08 0.18 0.11 0.09 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 1 1 1 0 1 1 1 1 0 0 1 0 23 8 11 7 6 7 2 1 3
Bud 2 2 3 0 0 4 1 6 3 6 9 5 2 3 1 15 3 1 1 3 3 3 2 6
Bud Neck 0 0 1 0 0 1 2 1 0 0 0 0 0 0 0 0 1 0 5 6 4 1 3 2
Bud Site 0 0 0 1 0 0 0 0 1 1 0 1 1 0 0 0 0 0
Cell Periphery 1 1 1 0 1 0 1 1 4 3 3 2 0 3 3 13 4 5 1 1 0 0 0 2
Cytoplasm 210 266 145 142 155 225 225 251 131 205 102 149 126 311 233 226 99 81 184 232 99 69 147 111
Endoplasmic Reticulum 0 7 3 1 3 1 6 7 6 5 2 2 3 0 0 24 12 5 6 4 6 3 1 3
Endosome 0 1 0 0 0 0 0 2 0 0 0 1 0 3 2 10 2 3 3 4 2 2 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 2
Mitochondria 28 14 19 14 15 95 154 133 131 199 134 174 4 0 2 17 6 7 27 23 30 11 15 13
Nucleus 0 0 0 0 0 1 2 1 1 0 0 0 0 2 1 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 2 0 0 0 1 0 0 0 2 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 1 1 0 0 0 1 0 2 0 1 0 1 0 3 1 0 0 1 0 0 0 0
SpindlePole 1 1 0 2 1 0 3 2 0 3 0 3 0 1 0 9 1 2 0 0 0 1 1 1
Vac/Vac Membrane 0 1 1 0 1 1 6 6 1 7 4 7 0 1 1 7 1 1 10 22 8 2 1 11
Unique Cell Count 224 285 161 152 171 268 329 344 229 339 189 278 134 318 236 286 117 105 253 310 170 104 182 168
Labelled Cell Count 244 295 174 162 177 330 400 412 279 434 257 347 136 326 243 349 139 118 253 310 170 104 182 168


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.3 3.9 3.9 4.1 3.6 3.2 3.7 3.3 3.2 3.4 3.2 5.1 5.1 5.0 5.9 6.9 7.2 4.5 4.5 5.4
Std Deviation (1e-4) 0.5 0.6 1.1 0.9 1.1 0.9 0.7 0.9 0.7 0.7 1.0 0.7 1.1 1.5 1.5 1.3 1.6 1.1 1.1 1.5 1.6
Intensity Change (Log2) -0.03 0.05 -0.14 -0.28 -0.08 -0.27 -0.32 -0.23 -0.3 0.36 0.37 0.34 0.57 0.81 0.86 0.19 0.2 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3411 0.8697 0.8132 0.5854 0.1936 0.8163 0.549 0.742 0.6028 0.2199 0.3007 0.5225 1.9387 1.2812 1.8873 1.5456 1.3015 0.9555
Actin 0.0161 0.006 0.0139 0.0637 0.0005 0.0036 0.0471 0.0018 0.01 0.019 0.0139 0.01 0.1306 0.1473 0.0157 0.0009 0.0011 0.0009
Bud 0.0002 0.0085 0.003 0.0005 0.0001 0.0011 0.0005 0.0007 0.0003 0.0003 0.0065 0.0002 0.0015 0.0004 0.0004 0.0005 0.0093 0.0001
Bud Neck 0.0115 0.002 0.0071 0.0016 0.0005 0.0188 0.0012 0.0005 0.0002 0.0003 0.0004 0.0191 0.0019 0.0007 0.0004 0.0002 0.0002 0.0033
Bud Periphery 0.0004 0.0157 0.0038 0.0022 0.0005 0.0014 0.0011 0.0021 0.0004 0.0002 0.0299 0.0006 0.0022 0.0005 0.0007 0.0006 0.0071 0.0001
Bud Site 0.0067 0.0406 0.0034 0.0006 0.0003 0.0003 0.0027 0.0058 0.0031 0.0001 0.0089 0.0001 0.0068 0.0067 0.0007 0.0005 0.0004 0
Cell Periphery 0.0013 0.003 0.0007 0.0006 0.0006 0.0003 0.0006 0.0008 0.0003 0.0001 0.0004 0.0002 0.0014 0.0009 0.0007 0.0002 0.0002 0.0001
Cytoplasm 0.1963 0.2711 0.3475 0.2792 0.1567 0.4025 0.1319 0.2005 0.1964 0.3097 0.1822 0.2508 0.0692 0.1987 0.2682 0.3128 0.0562 0.3151
Cytoplasmic Foci 0.0289 0.0401 0.0209 0.013 0.008 0.0198 0.0381 0.0117 0.0265 0.0439 0.0324 0.0268 0.1059 0.0402 0.0044 0.0418 0.0126 0.0108
Eisosomes 0.0007 0.0006 0.0006 0.0008 0.0004 0.0001 0.0017 0.0003 0.0003 0.0002 0.0002 0.0002 0.0018 0.001 0.0007 0.0007 0.0002 0.0001
Endoplasmic Reticulum 0.0042 0.0045 0.0029 0.0065 0.0015 0.0047 0.0074 0.0026 0.0021 0.002 0.0011 0.0015 0.009 0.0068 0.0169 0.0014 0.0022 0.0022
Endosome 0.0142 0.0069 0.0064 0.0222 0.0019 0.0076 0.0318 0.0057 0.0185 0.0079 0.0243 0.0096 0.1133 0.0293 0.0113 0.0007 0.0006 0.0061
Golgi 0.0035 0.0008 0.0011 0.0023 0.0002 0.0004 0.0177 0.0014 0.0043 0.001 0.0086 0.001 0.03 0.0071 0.0131 0.0003 0.0002 0.0002
Lipid Particles 0.0183 0.0069 0.0013 0.0009 0.0011 0.0018 0.0241 0.0009 0.0031 0.0007 0.0241 0.0033 0.0507 0.0248 0.001 0.0013 0.0008 0.0005
Mitochondria 0.0018 0.011 0.0022 0.0042 0.0005 0.0004 0.0109 0.002 0.0016 0.0007 0.007 0.0006 0.0112 0.011 0.0255 0.0004 0.0021 0.0008
None 0.6795 0.5674 0.5729 0.5887 0.8231 0.5291 0.6406 0.7528 0.7215 0.6003 0.6311 0.6642 0.3375 0.5042 0.6353 0.6345 0.8605 0.655
Nuclear Periphery 0.0013 0.0015 0.0007 0.0019 0.0004 0.0009 0.0052 0.0006 0.0007 0.0005 0.001 0.0003 0.0135 0.0011 0.0018 0.0003 0.0074 0.0007
Nucleolus 0.0006 0.0032 0.0027 0.0006 0.0008 0.0004 0.0022 0.0003 0.0003 0.0001 0.0004 0.0001 0.0042 0.0005 0.0001 0.0001 0.0015 0.0001
Nucleus 0.0007 0.0019 0.0019 0.0016 0.0007 0.0015 0.0064 0.0009 0.0006 0.0004 0.0006 0.0003 0.007 0.0011 0.0006 0.0003 0.0106 0.0005
Peroxisomes 0.0061 0.0018 0.001 0.0006 0.0002 0.0003 0.0144 0.0012 0.0044 0.0102 0.0198 0.0086 0.0329 0.0134 0.0001 0.0009 0.0006 0.0002
Punctate Nuclear 0.0037 0.0017 0.0015 0.0005 0.0006 0.0018 0.0103 0.0004 0.0015 0.0004 0.0016 0.0015 0.0521 0.0017 0.0003 0.0009 0.0237 0.0004
Vacuole 0.0036 0.0044 0.004 0.0062 0.0012 0.0029 0.0031 0.0063 0.003 0.0015 0.0046 0.0008 0.0139 0.0022 0.0014 0.0005 0.0017 0.0022
Vacuole Periphery 0.0005 0.0004 0.0004 0.0014 0.0001 0.0002 0.0011 0.0005 0.0005 0.0003 0.0009 0.0002 0.0033 0.0005 0.0008 0.0001 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6531 7.1618 5.7975 9.2588 5.6658 10.1908 9.0516 6.8868 9.161 12.9716
Translational Efficiency 0.6881 0.4131 0.7132 0.7665 1.4847 1.1001 0.4472 0.9098 0.9223 0.7168

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tda11

Tda11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available