Standard name | |
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Human Ortholog | |
Description | DNA binding protein with possible chromatin-reorganizing activity; involved in transcriptional activation, gene silencing, and DNA replication and repair |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 2 | – | – | – | 2 | 0 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 3 | – | 2 | 3 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 10 | – | 2 | 5 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 2 | 4 | 4 | 10 | 12 | 6 | 21 | 5 | – | 4 | 15 | 6 | 12 | 16 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 1 | 1 | – | 0 | 0 | 0 | 1 | 7 | – | – | – | 1 | 1 | 3 | 0 | 2 | 0 |
Golgi | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 1 | 0 |
Mitochondria | 0 | 10 | 1 | 1 | 1 | 5 | 8 | 0 | 18 | – | 32 | 6 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleus | 259 | 377 | 418 | 285 | 387 | 385 | 475 | 430 | 319 | – | 292 | 230 | 132 | 230 | 217 | – | – | – | 254 | 387 | 413 | 49 | 129 | 106 |
Nuclear Periphery | 0 | 3 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | – | 4 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 79 | 99 | 49 | 42 | 72 | 104 | 174 | 174 | 202 | – | 245 | 167 | 61 | 69 | 100 | – | – | – | 30 | 44 | 45 | 17 | 52 | 66 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 1 | 4 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 5 | 0 | – | 2 | 0 | 0 | 5 | 2 | – | – | – | 0 | 0 | 3 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 1 | 4 | 0 | 1 | 3 | 3 | 3 | 0 | – | 1 | 2 | 4 | 1 | 1 | – | – | – | 4 | 8 | 7 | 7 | 20 | 28 |
Unique Cell Count | 285 | 419 | 443 | 307 | 437 | 456 | 583 | 550 | 434 | 438 | 350 | 153 | 268 | 271 | 297 | 448 | 480 | 78 | 213 | 211 | ||||
Labelled Cell Count | 346 | 493 | 482 | 336 | 476 | 516 | 678 | 636 | 560 | 584 | 433 | 203 | 319 | 349 | 297 | 448 | 480 | 78 | 213 | 211 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.9 | 18.7 | 13.8 | 12.5 | 13.2 | 12.2 | 12.0 | 12.8 | 11.5 | – | 10.5 | 12.3 | 17.6 | 18.4 | 17.1 | – | – | – | 16.3 | 15.4 | 16.3 |
Std Deviation (1e-4) | 4.0 | 5.1 | 3.1 | 3.0 | 2.9 | 3.0 | 2.8 | 3.1 | 3.2 | – | 3.2 | 3.3 | 4.3 | 5.2 | 5.1 | – | – | – | 6.3 | 3.2 | 4.3 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.06 | -0.17 | -0.2 | -0.11 | -0.26 | – | -0.38 | -0.16 | 0.36 | 0.42 | 0.31 | – | – | – | 0.24 | 0.17 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0 | 1.6 | 2.0 | 0 | 2.9 | 0 | – | 0 | 3.1 | 0 | 3.1 | 3.9 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.0 | – | 5.5 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | -0.8 | -3.1 | -4.8 | -6.1 | -7.2 | -8.4 | – | -10.4 | -10.4 | -3.2 | -3.9 | -5.9 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 1.1 | 2.3 | 4.7 | 7.2 | 7.7 | 11.6 | – | 14.1 | 11.5 | 7.9 | 5.1 | 8.2 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.8482 | 9.4452 | 9.1185 | 8.0668 | 7.2756 | 8.4619 | 12.2541 | 11.4233 | 12.088 | 11.5818 | 12.0743 | 11.7249 | 12.4902 | 11.1344 | 11.1747 | 10.0335 | 10.6594 | 10.5765 |
Actin | 0.0057 | 0 | 0.0006 | 0.004 | 0.0573 | 0.0011 | 0.0179 | 0.0033 | 0.0066 | 0 | 0 | 0.0005 | 0.0373 | 0 | 0 | 0.0004 | 0.0004 | 0.0124 |
Bud | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0002 |
Bud Neck | 0.0003 | 0 | 0.0021 | 0.001 | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0009 | 0 | 0 | 0.0002 | 0.0001 | 0.0004 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0009 | 0.0001 | 0.001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0003 |
Bud Site | 0.0002 | 0 | 0.0029 | 0.0005 | 0.0014 | 0.0002 | 0.0042 | 0.0032 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0002 |
Cell Periphery | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.0052 | 0 | 0.0003 | 0.0002 | 0.0011 | 0.0008 | 0.0159 | 0.0001 | 0.0023 | 0.0003 | 0 | 0.0002 | 0.0005 | 0 | 0.0004 | 0.0056 | 0.0006 | 0.002 |
Cytoplasmic Foci | 0.0037 | 0 | 0.0006 | 0.0006 | 0.0105 | 0.0011 | 0.0172 | 0.0002 | 0.0015 | 0.0001 | 0 | 0.0004 | 0.0102 | 0 | 0.0006 | 0.0176 | 0.0275 | 0.0022 |
Eisosomes | 0 | 0 | 0 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0013 | 0 | 0.0002 | 0.0011 | 0.0032 | 0.0011 | 0.003 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0003 | 0.0048 |
Endosome | 0.0047 | 0 | 0.0043 | 0.007 | 0.0342 | 0.0042 | 0.0204 | 0.0017 | 0.0023 | 0.0001 | 0 | 0.0009 | 0.0022 | 0 | 0.0001 | 0.0008 | 0.0026 | 0.0052 |
Golgi | 0.0009 | 0 | 0.0008 | 0.0022 | 0.016 | 0.0005 | 0.0029 | 0.0004 | 0.0024 | 0 | 0 | 0.0001 | 0.0039 | 0 | 0 | 0.0004 | 0.0009 | 0.001 |
Lipid Particles | 0.0015 | 0 | 0.0044 | 0.0031 | 0.0719 | 0.0033 | 0.0112 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0076 | 0 | 0.0006 | 0.018 | 0.0193 | 0.0017 |
Mitochondria | 0.0008 | 0.0001 | 0.0004 | 0.0284 | 0.0219 | 0.0004 | 0.0064 | 0.001 | 0.0008 | 0.0001 | 0.0015 | 0.0004 | 0.0032 | 0 | 0.0021 | 0.0022 | 0.0019 | 0.0006 |
None | 0.004 | 0 | 0.0007 | 0.0007 | 0.0042 | 0.0008 | 0.0073 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.001 | 0 | 0.0097 | 0.0024 | 0.0018 | 0.0004 |
Nuclear Periphery | 0.0097 | 0.0018 | 0.0019 | 0.0024 | 0.0059 | 0.0087 | 0.0201 | 0.0019 | 0.0034 | 0.0034 | 0.0057 | 0.0053 | 0.0018 | 0.0001 | 0.0008 | 0.0047 | 0.0011 | 0.0036 |
Nucleolus | 0.0716 | 0.0333 | 0.0378 | 0.0738 | 0.378 | 0.2341 | 0.093 | 0.0338 | 0.032 | 0.0264 | 0.3714 | 0.1707 | 0.1051 | 0.0235 | 0.0406 | 0.0311 | 0.2824 | 0.1444 |
Nucleus | 0.8752 | 0.9642 | 0.9386 | 0.8704 | 0.3683 | 0.7351 | 0.7345 | 0.952 | 0.9452 | 0.938 | 0.6196 | 0.8099 | 0.7922 | 0.9761 | 0.9423 | 0.8856 | 0.6243 | 0.8128 |
Peroxisomes | 0.0002 | 0 | 0.0004 | 0.0007 | 0.015 | 0.0012 | 0.0106 | 0.0002 | 0.0009 | 0 | 0 | 0.0002 | 0.0112 | 0 | 0.0001 | 0.0007 | 0.02 | 0.0007 |
Punctate Nuclear | 0.0136 | 0.0004 | 0.0006 | 0.0008 | 0.0036 | 0.0061 | 0.0224 | 0.0006 | 0.0011 | 0.0313 | 0.0004 | 0.0097 | 0.0177 | 0.0003 | 0.0003 | 0.0244 | 0.0143 | 0.0052 |
Vacuole | 0.0005 | 0 | 0.0021 | 0.0011 | 0.0024 | 0.0004 | 0.0072 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0003 | 0.0011 | 0 | 0.0006 | 0.0015 | 0.0009 | 0.0015 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.001 | 0.001 | 0.0023 | 0.0004 | 0.0028 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0003 | 0.0015 | 0 | 0.0012 | 0.0012 | 0.0011 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.1772 | 29.6452 | 22.9979 | 27.8299 | 43.9737 | 34.6506 | 37.0136 | 32.6448 | 25.6005 | 38.3603 |
Translational Efficiency | 1.286 | 1.5239 | 1.3631 | 1.0829 | 1.0466 | 1.1705 | 0.9841 | 1.2557 | 1.2196 | 1.0566 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |