Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.08 0.07 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 0.98 0.96 0.94 0.84 0.79 0.81 0.78 0.79 0.76 0.97 0.99 1.0 0.99 0.95 0.99 0.97 0.94 0.88 0.91 0.88 0.88
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.12 0.15 0.13 0.13 0.09 0.11 0 0 0 0.06 0.09 0.06 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 5 9 9 4 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 2 0 2 4 4 8 2 6 4 8 1 1 1 2 3 1 0 0 2 0 1 0
Cytoplasm 73 127 150 104 132 118 134 117 96 104 64 66 95 114 141 287 154 161 75 135 133 83 127 107
Endoplasmic Reticulum 0 0 0 0 3 6 19 23 16 17 7 10 2 0 0 17 14 10 0 0 5 3 5 8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 4 3 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 1 3 5 9 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 0 0 0 0 0 0 2 0 1 0 0 1 3 1 0 2 0 0 4 0
Unique Cell Count 73 127 151 106 137 126 159 149 119 133 81 87 98 115 141 291 162 163 78 144 151 92 145 122
Labelled Cell Count 73 129 152 106 137 130 159 159 129 145 92 94 99 116 142 308 178 174 78 144 151 92 145 122


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 132.5 169.9 124.1 122.8 97.1 74.2 61.3 58.9 54.3 53.1 42.8 52.4 134.1 135.1 135.6 265.5 250.5 252.2 135.3 140.3 156.3
Std Deviation (1e-4) 24.4 33.0 29.8 32.1 23.5 18.3 19.7 16.4 14.5 14.7 12.7 15.5 35.3 34.0 36.1 66.6 92.8 75.9 31.8 44.0 51.6
Intensity Change (Log2) -0.02 -0.35 -0.74 -1.02 -1.08 -1.19 -1.22 -1.53 -1.24 0.11 0.12 0.13 1.1 1.01 1.02 0.12 0.18 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.8 -2.7 -4.8 -5.8 -5.4 -5.8 -5.5 -6.0 -1.5 -0.2 1.0 -0.7 -2.3 -0.5
Endoplasmic Reticulum 0 0 0 4.4 5.0 4.6 4.5 0 0 0 0 0 3.0 3.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 150.6706 204.6316 182.9845 167.6963 156.0514 127.3431 143.6659 203.9692 174.9257 151.5311 142.5723 134.4407 103.434 177.9097 161.4986 207.4217 118.5159 156.5716
Actin 0.0034 0.0013 0.0017 0.0049 0.0008 0.0018 0.0154 0.0004 0.0019 0.0014 0.001 0.0024 0.034 0.032 0.0181 0.0021 0.0053 0.0137
Bud 0.0046 0.0034 0.0034 0.0068 0.0032 0.0084 0.0031 0.0015 0.004 0.0023 0.0032 0.0013 0.0059 0.002 0.0088 0.0043 0.0126 0.0187
Bud Neck 0.0012 0.0009 0.0014 0.0017 0.0012 0.0024 0.0025 0.0011 0.0026 0.0014 0.0034 0.0078 0.0012 0.0014 0.0012 0.0029 0.0011 0.0056
Bud Periphery 0.0063 0.0023 0.0029 0.0088 0.0024 0.0038 0.0088 0.0015 0.0064 0.002 0.0019 0.0009 0.0067 0.0013 0.0082 0.0059 0.0221 0.0373
Bud Site 0.002 0.0023 0.0018 0.0017 0.0009 0.0012 0.0041 0.0093 0.0024 0.0007 0.0012 0.0004 0.0032 0.0018 0.0049 0.0018 0.0023 0.0044
Cell Periphery 0.0722 0.0608 0.0833 0.0734 0.0129 0.008 0.1194 0.0686 0.1016 0.0703 0.0141 0.0313 0.032 0.0384 0.0208 0.151 0.0455 0.0523
Cytoplasm 0.487 0.6792 0.5032 0.2577 0.3784 0.4963 0.349 0.693 0.5939 0.3767 0.4425 0.6179 0.3009 0.6926 0.5881 0.2565 0.1637 0.3026
Cytoplasmic Foci 0.018 0.0015 0.0024 0.0018 0.0235 0.0025 0.0185 0.0007 0.0136 0.0045 0.0533 0.0026 0.0208 0.0187 0.0052 0.001 0.004 0.0028
Eisosomes 0.0004 0.0001 0.0001 0.0003 0.001 0 0.0015 0 0.0003 0.0023 0.0002 0 0.001 0.0007 0.0009 0.0027 0.0002 0.0039
Endoplasmic Reticulum 0.028 0.0046 0.014 0.0066 0.0058 0.0137 0.0285 0.0065 0.0099 0.0043 0.0067 0.0376 0.0575 0.0044 0.0088 0.0027 0.014 0.0088
Endosome 0.0094 0.0037 0.0093 0.0084 0.0299 0.0325 0.0132 0.0016 0.0073 0.0055 0.0677 0.0168 0.0169 0.0036 0.0178 0.0019 0.0413 0.0068
Golgi 0.002 0.0007 0.0013 0.0012 0.0017 0.0009 0.0029 0.0002 0.0011 0.0007 0.0104 0.0018 0.0051 0.002 0.0058 0.0006 0.0078 0.0015
Lipid Particles 0.0046 0.002 0.0026 0.0028 0.0067 0.0006 0.0091 0.002 0.0075 0.0066 0.0069 0.0064 0.0089 0.0042 0.003 0.006 0.0038 0.0054
Mitochondria 0.0128 0.0029 0.0036 0.0036 0.0219 0.0042 0.0161 0.0005 0.0084 0.0338 0.0102 0.002 0.0156 0.0037 0.0355 0.0062 0.1542 0.0789
None 0.0066 0.0006 0.001 0.0011 0.0097 0.0016 0.0076 0.0003 0.0059 0.0058 0.0066 0.0009 0.0094 0.0013 0.0216 0.0016 0.0032 0.0063
Nuclear Periphery 0.0338 0.0068 0.0114 0.0127 0.022 0.1575 0.0432 0.0062 0.0086 0.0208 0.0577 0.0874 0.0953 0.0185 0.0191 0.0285 0.0279 0.0383
Nucleolus 0.0052 0.0015 0.0028 0.0041 0.0248 0.0011 0.0103 0.0012 0.0133 0.0143 0.0162 0.001 0.0108 0.002 0.0099 0.0872 0.046 0.025
Nucleus 0.0179 0.0067 0.0105 0.0187 0.0143 0.0845 0.0168 0.0055 0.0119 0.0142 0.0122 0.0566 0.1315 0.0186 0.0141 0.0433 0.0865 0.1404
Peroxisomes 0.0005 0.0002 0.0002 0.0002 0.0016 0.0004 0.0015 0.0001 0.0007 0.0016 0.0016 0.0002 0.0012 0.0058 0.0014 0.0006 0.0006 0.002
Punctate Nuclear 0.0023 0 0.0003 0.0008 0.0003 0.0015 0.0017 0 0.002 0.0002 0.0011 0.0012 0.058 0.0429 0.0003 0.0001 0.0003 0.0006
Vacuole 0.2206 0.1877 0.2796 0.4301 0.3049 0.1252 0.2861 0.1853 0.1758 0.3446 0.2063 0.1034 0.1315 0.088 0.1318 0.288 0.2136 0.1432
Vacuole Periphery 0.0613 0.031 0.0632 0.1524 0.1321 0.0522 0.0406 0.0146 0.0207 0.0862 0.0753 0.0198 0.0526 0.0163 0.0748 0.1049 0.1441 0.1015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3103.1495 2888.8524 3110.2525 3335.7128 3354.4759 2250.4846 2849.5423 2656.9654 2678.1639 2895.0607
Translational Efficiency 0.8168 0.8782 0.9055 0.6259 0.8752 1.1078 0.8811 0.8596 0.8853 0.9393

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2121 300 152 1650 1928 1419 2385 1584 4049 1719 2537 3234

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8491.04 11768.76 14482.96 11009.48 9252.39 9942.57 13043.22 10991.66 8853.57 10261.28 13129.48 11000.75
Standard Deviation 1784.70 2235.04 2397.99 2828.32 1792.21 2086.66 2464.53 2949.54 1828.26 2224.08 2484.21 2888.34
Intensity Change Log 2 0.470949 0.770343 0.374733 0.103793 0.495402 0.248511 0.291163 0.633519 0.310293

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000022 0.000152 0.000162 0.000189 0.000033 0.000065 0.000076 0.000368 0.000027 0.000080 0.000081 0.000277
Bud Neck 0.000081 0.001522 0.005036 0.000509 0.000192 0.000875 0.000429 0.001057 0.000134 0.000988 0.000705 0.000778
Bud Site 0.001173 0.011188 0.002706 0.003399 0.000795 0.002807 0.001976 0.007129 0.000993 0.004270 0.002020 0.005226
Cell Periphery 0.009687 0.055777 0.071418 0.048017 0.017356 0.010258 0.024642 0.046159 0.013339 0.018202 0.027445 0.047107
Cytoplasm 0.769820* 0.374028* 0.166191* 0.622639* 0.641310* 0.677864* 0.646845* 0.587807* 0.708628* 0.624838* 0.618047* 0.605578*
Cytoplasmic Foci 0.009482 0.020498 0.022607 0.002858 0.008024 0.011686 0.006129 0.007618 0.008788 0.013224 0.007116 0.005189
Eisosomes 0.000001 0.000005 0.000019 0.000003 0.000001 0.000001 0.000002 0.000005 0.000001 0.000002 0.000003 0.000004
Endoplasmic Reticulum 0.001351 0.000750 0.000266 0.000501 0.001510 0.001094 0.001288 0.000455 0.001427 0.001034 0.001227 0.000478
Endosome 0.000411 0.001439 0.000588 0.000270 0.000435 0.000740 0.000437 0.000880 0.000423 0.000862 0.000446 0.000569
Golgi 0.048519 0.146192* 0.068194 0.022739 0.083112 0.065921 0.038595 0.033545 0.064991 0.079930 0.040368 0.028031
Lipid Particles 0.007282 0.028028 0.039077 0.019544 0.012471 0.011089 0.016397 0.029038 0.009753 0.014045 0.017755 0.024194
Mitochondria 0.000188 0.000146 0.000137 0.000431 0.000129 0.000249 0.000299 0.000571 0.000160 0.000231 0.000289 0.000499
Mitotic Spindle 0.000577 0.003202 0.001793 0.000437 0.000827 0.001410 0.000486 0.000819 0.000696 0.001723 0.000565 0.000624
None 0.002153 0.001770 0.001166 0.003531 0.002163 0.002319 0.001684 0.004419 0.002158 0.002223 0.001653 0.003966
Nuclear Periphery 0.000435 0.001802 0.005854 0.000593 0.001059 0.000962 0.000758 0.000644 0.000732 0.001108 0.001063 0.000618
Nuclear Periphery Foci 0.008858 0.015852 0.020528 0.006921 0.016678 0.015952 0.009249 0.007890 0.012582 0.015934 0.009925 0.007396
Nucleolus 0.000041 0.000106 0.000152 0.000161 0.000048 0.000136 0.000083 0.000204 0.000044 0.000130 0.000087 0.000182
Nucleus 0.003182 0.009769 0.019963 0.011568 0.004759 0.010317 0.006458 0.014420 0.003933 0.010221 0.007267 0.012965
Peroxisomes 0.000017 0.000035 0.000013 0.000047 0.000008 0.000011 0.000038 0.000077 0.000013 0.000015 0.000037 0.000061
Vacuole 0.043596 0.162449 0.425475* 0.195823 0.077240 0.098676 0.160750 0.205946* 0.059616 0.109806 0.176611 0.200781*
Vacuole Periphery 0.093125 0.165290* 0.148654* 0.059820 0.131849* 0.087569 0.083377 0.050951 0.111564* 0.101133* 0.087288 0.055476

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.34 -6.87 -4.49 -0.96 -0.37 -5.16 -4.75 -3.01 -2.68 -2.57 -7.33 -6.25 -4.33 -3.34 -3.26
Bud Neck -6.77 -5.47 -8.90 4.35 4.97 -4.48 -4.43 -10.42 -1.20 -6.50 -6.16 -7.37 -13.34 1.16 -1.33
Bud Site -3.56 -2.78 -6.05 2.65 -0.77 -5.07 -7.15 -9.51 -5.74 -7.59 -5.57 -6.56 -11.20 -1.77 -7.98
Cell Periphery -9.97 -11.35 -18.11 1.58 4.05 5.70 -4.96 -11.74 -15.72 -8.44 -4.04 -11.58 -21.13 -15.49 -10.34
Cytoplasm 18.77 30.12 14.28 -11.24 -21.68 -2.63 -0.09 4.88 7.08 5.19 8.74 10.87 13.48 1.72 1.24
Cytoplasmic Foci -5.61 -4.19 8.87 9.02 5.81 -4.71 2.48 -0.32 3.84 -2.24 -6.03 1.63 5.72 10.02 3.46
Eisosomes -5.97 -5.21 -8.65 2.15 4.39 -1.55 -3.09 -5.95 -5.43 -4.38 -6.46 -6.71 -9.36 -5.12 -2.33
Endoplasmic Reticulum 3.46 7.62 6.27 1.94 -3.66 0.90 0.77 5.07 1.46 3.49 1.09 0.89 7.77 1.52 3.32
Endosome -5.20 -3.80 4.31 5.92 6.51 -6.20 -0.09 -1.72 -0.29 -1.71 -8.25 -1.02 -1.08 2.69 -0.85
Golgi -11.29 -3.50 12.30 14.65 9.67 4.92 16.02 17.85 11.54 3.63 -4.62 12.63 21.24 18.71 8.63
Lipid Particles -7.83 -10.52 -9.22 3.05 6.19 1.14 -3.63 -9.52 -10.21 -6.89 -4.99 -8.69 -13.25 -7.76 -4.73
Mitochondria 1.70 1.53 -2.27 -2.62 -2.58 -4.01 -5.18 -3.94 -2.63 -2.39 -3.08 -4.31 -4.40 -3.09 -2.54
Mitotic Spindle -3.07 -4.00 1.17 3.20 4.21 -4.54 5.74 0.15 3.58 -2.40 -5.60 2.26 0.87 5.56 -0.32
None 1.64 4.19 -1.81 -2.34 -3.11 -1.05 1.81 -2.42 -2.18 -2.85 -0.57 1.96 -3.01 -2.81 -3.64
Nuclear Periphery -8.22 -11.10 -4.39 7.26 10.76 0.36 4.66 6.78 4.19 2.72 -5.48 -6.29 3.04 7.02 8.71
Nuclear Periphery Foci -5.35 -4.90 3.13 6.70 5.66 0.43 9.56 11.49 9.41 3.06 -4.49 4.66 10.67 11.55 5.72
Nucleolus -8.46 -8.82 -6.11 -2.52 0.21 -2.09 -8.03 -3.05 -0.34 -2.31 -2.37 -10.33 -5.10 -0.29 -3.34
Nucleus -3.77 -5.84 -9.79 -0.81 3.18 -5.67 -2.94 -10.47 -4.41 -8.58 -7.64 -6.50 -14.28 -3.34 -7.52
Peroxisomes -2.91 0.87 -4.96 -1.48 -5.49 -1.49 -7.24 -5.33 -5.08 -2.72 -1.06 -6.00 -6.96 -6.59 -3.00
Vacuole -11.31 -19.79 -25.81 -2.80 11.24 -4.71 -16.69 -20.48 -15.32 -6.55 -12.23 -25.47 -32.72 -16.30 -4.27
Vacuole Periphery -6.93 -3.63 8.49 10.62 6.53 8.21 10.39 18.36 9.36 11.21 2.68 7.33 19.05 12.10 11.49