Standard name
Human Ortholog
Description Protein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.99 0.98 0.93 0.87 0.88 0.78 0.7 0.68 0.76 0.99 0.98 0.98 0.97 0.97 0.96 0.96 0.97 0.95 0.96 0.94 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.13 0.11 0.28 0.28 0.4 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.05 0.09 0.07 0.09 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0
Bud 0 0 0 2 0 0 1 3 4 3 6 4 0 0 0 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 3 0 5 2 1 5 2 2 2 4 5 5 3 6 9 8 14 0 0 0 0 0 0
Cytoplasm 182 231 43 178 283 166 289 299 198 179 168 252 406 448 457 168 132 159 174 224 78 304 550 532
Endoplasmic Reticulum 1 0 0 0 0 2 1 0 1 1 1 5 2 1 2 4 3 1 0 1 1 1 3 3
Endosome 0 0 0 0 0 0 0 1 1 0 0 2 1 1 1 0 0 0 1 1 0 0 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 2 1
Mitochondria 2 0 0 2 0 14 43 37 72 71 99 76 1 0 1 0 0 1 3 0 0 0 2 1
Nucleus 0 0 1 1 0 3 3 2 7 13 8 8 4 2 5 1 1 5 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 2 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 0 0 6 5 13 22 12 24 17 30 1 3 1 3 8 2 0 0 0 3 7 1
Unique Cell Count 184 231 44 180 289 178 332 339 254 256 248 330 412 455 465 173 136 165 182 231 83 316 584 550
Labelled Cell Count 186 234 44 188 292 191 356 366 300 296 303 383 420 459 474 187 152 183 182 231 83 316 584 550


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 9.6 8.3 7.8 7.8 6.0 5.8 6.2 5.5 5.4 5.1 5.7 9.3 9.4 9.1 11.3 13.7 13.3 9.2 9.3 10.1
Std Deviation (1e-4) 0.9 1.3 0.9 1.1 1.3 1.2 1.3 1.5 1.1 1.1 1.1 2.1 1.4 1.4 1.4 2.3 2.8 2.6 1.3 1.4 1.4
Intensity Change (Log2) -0.09 -0.09 -0.45 -0.5 -0.41 -0.6 -0.6 -0.7 -0.53 0.17 0.18 0.13 0.45 0.72 0.69 0.16 0.17 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.1 -1.1 -2.1 -1.9 -3.1 -3.9 -4.1 -3.3 0.4 0.4 0.3 -0.2 -0.2 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.5 0 4.1 4.0 5.2 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6478 5.0044 4.6552 3.8185 3.8936 4.4089 4.1936 5.9729 5.1606 4.5606 4.9561 5.4315 4.0272 5.3108 4.3516 4.4253 3.9074 4.5665
Actin 0.0108 0.0004 0.0049 0.0009 0.0047 0.0027 0.0093 0.0009 0.0073 0.0045 0.0238 0.007 0.0141 0.001 0.0172 0.0019 0.0153 0.0012
Bud 0.0002 0.0006 0.0004 0.0007 0.0006 0.0003 0.0004 0.0011 0.0023 0.0002 0.0003 0.0004 0.0003 0.0063 0.002 0.0222 0.0033 0.0008
Bud Neck 0.0028 0.0002 0.0017 0.0005 0.0013 0.0009 0.0009 0.0001 0.001 0.0005 0.0006 0.0013 0.0014 0.0002 0.0023 0.0007 0.0016 0.001
Bud Periphery 0.0002 0.0003 0.0003 0.0003 0.0005 0.0003 0.0004 0.0004 0.0013 0.0002 0.0003 0.0002 0.0003 0.0023 0.0019 0.003 0.0037 0.0003
Bud Site 0.0017 0.0046 0.001 0.001 0.0006 0.0002 0.0029 0.0026 0.0108 0.0003 0.0012 0.0002 0.0012 0.0075 0.0048 0.0037 0.0039 0.0001
Cell Periphery 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0004 0.0001 0 0.0001 0.0002 0.0002 0.0005 0.0002 0
Cytoplasm 0.8399 0.9647 0.9179 0.8961 0.9073 0.9219 0.8748 0.9782 0.9054 0.9684 0.8905 0.9536 0.9027 0.9598 0.8933 0.889 0.8515 0.959
Cytoplasmic Foci 0.0265 0.0038 0.0155 0.0072 0.0172 0.0131 0.0227 0.0022 0.0295 0.0041 0.0117 0.009 0.0161 0.0042 0.0094 0.008 0.0115 0.0083
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0021 0.0011 0.002 0.0024 0.0033 0.0028 0.0054 0.0007 0.0023 0.0062 0.0038 0.0016 0.0025 0.0013 0.0021 0.002 0.005 0.0012
Endosome 0.018 0.0021 0.0078 0.0049 0.0175 0.0103 0.0263 0.0006 0.0133 0.0045 0.0218 0.0083 0.0153 0.0064 0.009 0.0104 0.0379 0.0071
Golgi 0.0035 0.0001 0.0025 0.0002 0.0021 0.0015 0.0114 0.0001 0.003 0.0015 0.0107 0.0023 0.0051 0.0003 0.004 0.0021 0.0087 0.0022
Lipid Particles 0.0085 0 0.0003 0.0001 0.003 0.0005 0.0042 0 0.0023 0.0013 0.0103 0.0005 0.0037 0 0.0029 0.0226 0.0178 0.0004
Mitochondria 0.0093 0.0001 0.0028 0.0003 0.0017 0.0009 0.0095 0.0001 0.0008 0.0004 0.0053 0.0008 0.0019 0.0003 0.0145 0.002 0.0066 0.0006
None 0.0524 0.0144 0.0351 0.076 0.012 0.0334 0.0182 0.0107 0.0074 0.0041 0.0042 0.0046 0.0167 0.0067 0.0163 0.0079 0.0058 0.0117
Nuclear Periphery 0.0011 0.0005 0.0011 0.0015 0.0033 0.0027 0.0027 0.0003 0.0008 0.0007 0.0052 0.0008 0.0017 0.0005 0.0019 0.0025 0.007 0.0007
Nucleolus 0.0005 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0003 0.0001 0.0008 0.001 0.0004 0.0001
Nucleus 0.0051 0.0049 0.0029 0.0044 0.0042 0.0042 0.0028 0.0013 0.0018 0.0015 0.0023 0.0019 0.0054 0.0019 0.0025 0.0115 0.0043 0.0027
Peroxisomes 0.0128 0.0001 0.0012 0.0001 0.0129 0.0005 0.0031 0.0001 0.0055 0.0001 0.0037 0.0063 0.005 0.0001 0.0064 0.0036 0.0033 0.0002
Punctate Nuclear 0.0026 0.0014 0.0013 0.0016 0.0046 0.0023 0.0024 0.0003 0.0043 0.0002 0.0019 0.0007 0.0043 0.0004 0.0038 0.0029 0.0046 0.0013
Vacuole 0.0014 0.0006 0.0009 0.0014 0.0022 0.0009 0.0017 0.0004 0.0005 0.0009 0.0008 0.0005 0.0015 0.0006 0.0039 0.0017 0.0058 0.0009
Vacuole Periphery 0.0003 0.0001 0.0002 0.0002 0.0007 0.0003 0.0006 0 0.0001 0.0002 0.0008 0.0001 0.0003 0.0001 0.0007 0.0007 0.0017 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.7976 14.6595 21.756 27.2558 37.2081 26.926 27.7917 26.7964 23.126 36.1814
Translational Efficiency 1.2319 2.4354 1.4307 0.8343 0.8319 1.1069 1.2095 1.0932 1.2449 1.189

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
224 539 2802 1536 1855 496 131 1514 2079 1035 2933 3050

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 919.66 1084.70 1029.58 1046.46 918.06 1075.80 1210.41 1127.18 918.23 1080.43 1037.66 1086.53
Standard Deviation 115.78 141.55 131.70 143.96 122.13 135.71 145.39 142.73 121.46 138.85 137.51 148.92
Intensity Change Log 2 0.238124 0.162883 0.186345 0.228750 0.398835 0.296058 0.233448 0.285575 0.242196

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000128 0.000264 0.000868 0.000871 0.000108 0.000267 0.000540 0.006195 0.000110 0.000266 0.000854 0.003514
Bud Neck 0.005236 0.001657 0.001235 0.002099 0.001822 0.002644 0.001641 0.002630 0.002190 0.002130 0.001254 0.002363
Bud Site 0.009395 0.015306 0.005275 0.025052 0.005840 0.015605 0.006187 0.026386 0.006223 0.015450 0.005316 0.025714
Cell Periphery 0.000119 0.000074 0.000035 0.000127 0.000094 0.000075 0.000238 0.000074 0.000096 0.000075 0.000044 0.000101
Cytoplasm 0.632628* 0.766965* 0.785336* 0.675925* 0.738508* 0.687481* 0.451805* 0.691810* 0.727100* 0.728874* 0.770439* 0.683810*
Cytoplasmic Foci 0.158773 0.123508 0.008843 0.033677 0.134604 0.142068 0.023214 0.031414 0.137208 0.132402 0.009485 0.032553
Eisosomes 0.000051 0.000015 0.000006 0.000019 0.000026 0.000023 0.000081 0.000015 0.000028 0.000019 0.000010 0.000017
Endoplasmic Reticulum 0.001031 0.000640 0.000362 0.000801 0.002511 0.000684 0.004014 0.000370 0.002351 0.000661 0.000525 0.000587
Endosome 0.019106 0.006012 0.000555 0.006507 0.005919 0.014289 0.009040 0.011359 0.007340 0.009979 0.000934 0.008916
Golgi 0.005209 0.001360 0.000090 0.004756 0.001819 0.003473 0.000186 0.013879 0.002184 0.002373 0.000094 0.009285
Lipid Particles 0.001542 0.001002 0.000314 0.001182 0.001510 0.000734 0.003543 0.000391 0.001513 0.000873 0.000458 0.000789
Mitochondria 0.001535 0.002895 0.000206 0.002793 0.000455 0.000619 0.000158 0.002885 0.000571 0.001804 0.000204 0.002838
Mitotic Spindle 0.003476 0.001364 0.005188 0.023237 0.001781 0.005785 0.001522 0.028731 0.001964 0.003483 0.005025 0.025964
None 0.001407 0.000976 0.003893 0.001692 0.002360 0.000907 0.002526 0.002812 0.002257 0.000943 0.003832 0.002248
Nuclear Periphery 0.000138 0.000105 0.000273 0.000327 0.000105 0.000088 0.001384 0.000272 0.000108 0.000097 0.000323 0.000300
Nuclear Periphery Foci 0.001946 0.002503 0.000816 0.003307 0.001323 0.001719 0.014681 0.001674 0.001390 0.002128 0.001435 0.002497
Nucleolus 0.000270 0.000834 0.000084 0.000105 0.000399 0.000127 0.000218 0.000137 0.000385 0.000495 0.000090 0.000121
Nucleus 0.031485 0.018423 0.151526* 0.045919 0.042345 0.016176 0.142597* 0.041021 0.041175 0.017346 0.151127* 0.043488
Peroxisomes 0.000820 0.000897 0.000507 0.000592 0.000459 0.003244 0.000329 0.001716 0.000498 0.002022 0.000499 0.001150
Vacuole 0.124235 0.053284 0.034530 0.167247 0.056482 0.102421 0.335862* 0.127452 0.063782 0.076832 0.047988 0.147493
Vacuole Periphery 0.001470 0.001917 0.000056 0.003765 0.001531 0.001569 0.000233 0.008776 0.001524 0.001750 0.000064 0.006252

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.93 -1.48 -4.85 -2.41 0.49 -2.92 -6.01 -1.72 -1.48 -1.01 -4.91 -1.63 -2.15 -1.69 -0.67
Bud Neck 1.53 1.65 1.56 0.09 -0.85 -1.90 -1.50 0.33 1.99 1.62 0.24 2.33 1.67 1.45 -1.17
Bud Site -2.70 3.64 -1.93 1.03 -5.79 -5.53 -1.71 -3.94 1.95 -2.34 -6.95 2.07 -5.62 2.06 -6.92
Cell Periphery 4.25 8.50 3.01 -0.65 -4.48 2.28 -5.56 4.90 2.52 7.70 3.04 7.21 3.49 0.77 -4.08
Cytoplasm -6.21 -5.54 1.89 13.39 17.19 4.65 16.65 14.21 4.45 -11.09 0.79 1.80 17.40 12.27 17.20
Cytoplasmic Foci 2.98 15.05 13.58 15.42 -11.23 -0.53 29.25 31.86 17.70 -0.92 1.15 38.77 34.55 23.37 -13.01
Eisosomes 6.37 7.83 5.97 -1.96 -11.22 1.00 -6.97 7.26 3.03 8.36 5.30 12.35 8.65 1.47 -7.28
Endoplasmic Reticulum 1.46 2.49 2.21 0.72 -1.29 4.16 -2.49 5.20 2.52 8.06 4.33 4.53 5.10 2.17 5.18
Endosome 3.79 5.43 4.78 4.28 -4.74 -3.68 -1.12 4.19 5.02 3.91 -1.84 13.09 7.34 6.23 -5.28
Golgi 1.33 1.75 1.29 -0.20 -3.02 -1.14 13.26 -1.01 0.18 -2.66 0.04 6.36 0.12 0.07 -3.75
Lipid Particles 1.24 4.24 1.76 -0.20 -4.94 6.55 -4.47 8.22 0.20 6.80 3.01 10.17 6.04 -0.12 -4.20
Mitochondria -0.64 1.04 0.50 1.32 -1.73 -0.38 3.48 -0.83 -0.34 -2.01 -1.53 2.37 -0.73 1.22 -2.47
Mitotic Spindle 0.65 -0.77 -2.53 -5.68 -3.09 -1.42 -1.37 -5.57 -2.34 -1.83 -0.95 -3.22 -7.03 -5.24 -4.02
None 0.79 -4.30 -1.09 -4.65 7.84 4.81 0.95 -2.70 -8.75 -2.81 5.48 -5.85 -1.22 -9.68 6.83
Nuclear Periphery 0.64 -8.31 -8.21 -9.65 -0.60 0.35 -11.33 -17.71 -17.89 7.94 0.18 -21.16 -22.21 -17.45 0.20
Nuclear Periphery Foci -0.99 1.77 -0.64 0.49 -4.23 -1.73 -6.83 -2.21 0.49 6.64 -3.69 -1.07 -3.22 0.72 -2.36
Nucleolus -0.78 2.13 1.88 1.02 -2.83 1.34 0.92 1.24 -1.80 1.92 -0.13 1.50 1.37 0.99 -3.90
Nucleus 2.78 -28.65 -7.17 -16.19 29.94 14.24 -6.82 -10.77 -22.08 4.83 13.94 -40.71 -12.64 -26.51 32.10
Peroxisomes -0.50 2.47 4.22 4.96 0.53 -2.09 3.12 0.01 2.07 -1.10 -2.40 1.04 1.71 2.61 -0.09
Vacuole 5.36 5.00 -11.56 -26.93 -29.71 -7.79 -15.40 -28.31 -13.56 7.44 -4.86 -5.92 -38.51 -27.56 -32.86
Vacuole Periphery -0.39 3.68 0.62 0.81 -2.64 0.18 3.28 -0.37 -0.47 -2.53 -0.13 4.02 0.12 0.24 -3.74