Standard name
Description Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0.21 0.11 0.06 0 0.09 0 0 0.06 0 0 0.23 0.4 0.08 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0.1 0 0.07 0 0 0 0 0 0 0 0 0
Endosome 0.05 0.1 0.12 0.06 0.12 0.05 0 0 0 0 0 0.12 0.12 0.13 0.09 0.11 0.09 0.08 0.08 0.1 0.08 0.05 0.07
Golgi 0 0.05 0 0 0.07 0 0 0 0 0 0 0.29 0.13 0.17 0.24 0.3 0.24 0 0.09 0 0 0 0
Mitochondria 0.9 0.74 0.31 0.73 0.64 0.81 0.81 0.91 0.87 0.91 0.92 0.4 0.41 0.17 0.09 0.11 0.09 0.71 0.66 0.73 0.69 0.72 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0.06 0 0.09 0 0 0 0 0 0 0 0 0.09 0 0.17 0.15 0.22 0 0 0.05 0.05 0 0
SpindlePole 0 0 0.12 0.07 0 0 0 0 0.05 0 0 0 0 0 0.2 0.17 0.21 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.19 0.19 0.06 0.18 0.1 0.08 0.05 0.07 0.07 0 0.21 0.15 0.14 0.15 0.13 0.21 0.07 0.05 0 0.05 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 4 1 0 0 0 0 1 2 4 3 5 11 3 6 1 19 2 3 1 6
Bud 11 10 1 4 9 10 8 7 8 7 12 0 3 0 16 5 10 3 3 0 1 1 1
Bud Neck 2 0 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 1 3 0 1 2 1
Bud Site 2 1 0 0 0 0 4 3 2 7 5 0 0 0 1 2 8
Cell Periphery 5 17 3 4 5 6 3 1 3 4 1 1 3 7 9 14 12 0 0 0 0 0 0
Cytoplasm 15 17 27 17 15 11 30 10 11 20 5 2 29 55 19 18 15 1 3 2 2 4 1
Endoplasmic Reticulum 4 10 6 2 6 7 3 4 1 3 6 6 4 10 6 5 3 2 4 0 1 1 2
Endosome 18 44 15 10 30 15 15 1 10 0 0 7 15 18 20 26 22 31 40 24 13 10 18
Golgi 4 22 3 7 16 4 7 0 2 0 0 17 16 23 56 73 59 16 46 3 3 9 8
Mitochondria 330 322 40 117 156 234 271 229 225 296 276 23 52 23 20 26 22 274 352 169 122 156 184
Nucleus 3 0 2 2 2 8 10 5 10 3 8 0 1 2 4 6 2 1 1 0 0 1 2
Nuclear Periphery 3 6 1 1 2 5 7 3 4 12 8 1 0 0 0 4 0 3 3 0 0 0 3
Nucleolus 3 1 0 0 3 4 5 8 2 4 4 0 1 0 14 8 12 1 0 0 0 0 1
Peroxisomes 4 28 5 14 3 6 4 0 1 2 1 1 11 3 40 36 55 10 16 11 8 3 9
SpindlePole 9 11 15 12 7 7 14 8 13 8 9 2 4 3 48 42 51 4 3 1 2 3 4
Vac/Vac Membrane 46 84 25 9 43 29 28 12 19 22 13 12 19 19 36 31 52 25 24 10 8 15 15
Unique Cell Count 366 438 129 160 243 289 334 252 260 324 299 58 126 136 235 241 246 385 530 233 177 218 266
Labelled Cell Count 459 575 149 204 298 346 409 291 312 389 351 76 161 168 300 299 329 385 530 233 177 218 266


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.0 5.9 6.2 6.8 5.9 6.1 5.1 5.0 4.5 4.6 6.9 6.9 6.5 10.8 11.4 11.5 5.2 5.9 6.0
Std Deviation (1e-4) 0.7 1.1 1.1 1.7 1.4 1.4 1.7 1.5 1.2 1.4 0.9 0.9 1.2 1.1 2.6 3.0 3.0 1.1 1.3 1.4
Intensity Change (Log2) 0.09 0.22 0.0 0.05 -0.2 -0.24 -0.37 -0.34 0.23 0.24 0.15 0.88 0.96 0.97 -0.16 0.02 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.7 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.5 1.2
Cytoplasm -2.4 -4.3 -5.6 -3.5 -5.3 -5.2 -4.6 -7.0 -3.1 0.4 3.4 -3.5 -3.8 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.9 0 0 0
Endosome -1.6 0.2 -2.4 -2.8 -5.2 -2.9 0 0 0.1 0.1 0.4 -1.0 -0.2 -0.8
Golgi 0 1.8 0 0 0 0 0 0 5.5 3.2 4.0 5.3 6.3 5.4
Mitochondria 7.1 6.1 9.9 10.3 12.1 11.1 0 0 1.2 1.7 -2.7 -5.5 -4.8 -5.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.7 0 0 0 0 0 0 0 0 1.6 0 3.6 3.2 4.6
SpindlePole -1.2 -3.4 -3.9 -3.0 -3.3 -2.4 0 0 -1.8 -2.6 -3.0 2.1 1.5 2.2
Vacuole -3.6 -0.4 -2.6 -3.3 -4.6 -3.5 -4.0 -5.0 0.2 -0.9 -1.2 -1.0 -1.7 0.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gut2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available