Standard name
Human Ortholog
Description Essential chromatin-associated protein; involved in initiation of DNA replication; required for association of MCM2-7 complex with replication origins; required to stabilize catalytic subunit of DNA polymerase-alpha; self-associates through its N-terminal domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.07 0.1 0.15 0.15 0.22 0.21 0.17 0.26 0.1 0.14 0.12 0.2 0.25 0.11 0.1 0.15 0 0 0 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.06 0 0.1 0 0.22 0.24 0.13 0.51 0.36 0.74 0.5 0 0 0 0.11 0.05 0.06 0 0 0 0 0 0.05
Nucleus 0.87 0.82 0.87 0.78 0.74 0.6 0.52 0.61 0.53 0.51 0.44 0.48 0.86 0.72 0.68 0.69 0.71 0.65 0.78 0.78 0.67 0.67 0.45 0.37
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.09 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.08 0.06
Vac/Vac Membrane 0.07 0.16 0.07 0 0.09 0.14 0.11 0.07 0.07 0.08 0.05 0.09 0 0.05 0.05 0.12 0.13 0.13 0.1 0.11 0.19 0.16 0.25 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0
Bud 0 0 0 3 0 1 1 3 3 1 7 1 0 0 0 2 1 1 1 3 3 0 1 4
Bud Neck 0 0 1 0 0 0 1 6 2 0 2 1 0 0 0 0 1 0 0 0 2 0 1 3
Bud Site 1 0 0 1 0 0 2 2 5 3 6 3 0 0 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 15 16 24 41 49 76 67 40 59 26 40 25 47 80 25 22 37 2 3 5 1 10 8
Endoplasmic Reticulum 3 0 0 1 2 2 3 1 1 1 0 0 0 0 1 12 10 8 0 0 1 0 0 0
Endosome 0 1 1 0 2 8 8 3 0 1 0 2 3 8 9 9 10 10 2 0 2 1 3 5
Golgi 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Mitochondria 24 10 1 23 7 71 85 43 118 81 200 139 1 0 2 25 11 16 1 4 1 0 3 8
Nucleus 187 144 188 188 202 197 184 197 123 116 118 132 185 172 215 164 163 163 182 150 171 34 89 65
Nuclear Periphery 1 1 0 1 1 0 2 0 2 1 0 1 0 0 0 4 2 0 2 0 0 0 0 2
Nucleolus 0 0 0 2 1 3 1 1 1 2 2 1 2 4 1 8 11 8 6 4 5 0 18 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 1 2 9 7 7 11 13 7 7 2 10 11 9 10 4 4 5 1 3 8 1 15 10
Vac/Vac Membrane 15 28 14 5 25 46 39 23 16 18 14 26 5 11 17 28 30 32 24 21 48 8 50 51
Unique Cell Count 215 175 215 240 273 329 352 325 233 226 270 277 216 238 314 236 228 250 234 194 256 51 198 179
Labelled Cell Count 238 200 223 258 290 385 413 360 318 290 377 356 233 251 335 282 265 281 234 194 256 51 198 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.2 5.8 5.4 5.9 4.7 4.7 5.0 4.1 4.5 3.7 4.2 6.2 5.9 5.6 7.1 7.3 6.8 4.6 5.6 6.1
Std Deviation (1e-4) 0.6 1.1 0.8 1.1 1.4 1.2 1.2 1.1 0.9 1.5 1.0 1.2 1.3 1.9 1.3 1.4 1.7 1.6 0.7 1.2 1.4
Intensity Change (Log2) -0.12 0.01 -0.3 -0.32 -0.22 -0.53 -0.37 -0.66 -0.49 0.09 0.02 -0.06 0.28 0.32 0.21 -0.34 -0.05 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 2.6 2.6 4.4 4.2 3.1 5.2 0.9 2.4 1.5 3.8 5.3 1.2 0.8 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2.7 2.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 0 0 0 5.3 0 0 0 0 0 0 0 4.6 2.8 3.4
Nucleus -2.6 -3.7 -6.9 -8.6 -6.7 -8.0 -8.2 -9.9 -9.2 -0.5 -4.0 -5.0 -4.6 -4.1 -5.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.0 0 0 0 2.1 0 0 0 0 2.5 2.0 0 0 0 0
Vacuole -2.4 1.1 2.7 1.8 0.3 0.2 0.6 -0.6 1.2 -2.1 -0.9 -0.5 2.0 2.3 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.858 1.6967 1.3575 1.4082 1.6614 1.4936 0.9416 0.5896 0.8111 0.7077 1.338 0.5907 1.1077 1.072 0.687 0.9765 0.8466 0.7175
Actin 0.0074 0.0005 0.0076 0.0013 0.0055 0.0013 0.0372 0 0.0145 0.006 0.0086 0.0009 0.0389 0.0046 0.0069 0.0099 0 0.0076
Bud 0.0009 0.0001 0.0013 0.0001 0.0004 0.0001 0.0006 0 0.0006 0.001 0.0009 0.0002 0.0052 0.0021 0.0004 0.0002 0 0.0003
Bud Neck 0.0128 0.0003 0.0012 0.0003 0.002 0.0017 0.0033 0.0003 0.002 0.0029 0.0015 0.0032 0.01 0.0006 0.0007 0.0008 0.0002 0.0027
Bud Periphery 0.0015 0.0001 0.0017 0.0001 0.0004 0.0003 0.0012 0.0001 0.0011 0.0025 0.0016 0.0004 0.0025 0.0007 0.0004 0.0004 0 0.0009
Bud Site 0.01 0.0002 0.0106 0.0001 0.0014 0.0004 0.0046 0.0003 0.006 0.0037 0.0024 0.001 0.028 0.0033 0.0036 0.0017 0.0001 0.0006
Cell Periphery 0.0005 0 0.0002 0 0.0001 0.0001 0.0005 0 0.0004 0.0016 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001 0 0.0003
Cytoplasm 0.0155 0.0029 0.0046 0.0006 0.01 0.0008 0.0212 0.0095 0.0069 0.0042 0.0062 0.0016 0.0246 0.0084 0.0091 0.0018 0.003 0.013
Cytoplasmic Foci 0.0179 0.0015 0.0056 0.0031 0.0332 0.0034 0.0175 0.0002 0.0048 0.0049 0.0321 0.0011 0.0194 0.0006 0.0103 0.0019 0 0.0026
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0007 0 0.0002 0.0001 0.0001 0 0.0005 0 0 0.0002 0 0.0008
Endoplasmic Reticulum 0.0034 0.0002 0.0006 0.0003 0.007 0.0012 0.0094 0.0005 0.0043 0.0027 0.0035 0.0002 0.0137 0.0008 0.0006 0.0001 0.0001 0.0003
Endosome 0.0372 0.0015 0.013 0.0198 0.1022 0.0124 0.0316 0.0009 0.015 0.0235 0.0319 0.0021 0.0712 0.0051 0.0088 0.003 0.0001 0.0074
Golgi 0.0108 0.0009 0.0018 0.0228 0.0206 0.004 0.0093 0 0.0071 0.0073 0.0106 0.001 0.0136 0.0001 0.0098 0.0026 0 0.0114
Lipid Particles 0.0202 0.001 0.0025 0.0268 0.0244 0.0107 0.0214 0.0001 0.0039 0.0067 0.0092 0.0013 0.0273 0.0002 0.0066 0.0129 0 0.0062
Mitochondria 0.0152 0.0057 0.005 0.0049 0.0043 0.0056 0.008 0.0003 0.0144 0.0129 0.0534 0.0063 0.0267 0.0016 0.0119 0.0464 0.0004 0.0232
None 0.0184 0.0012 0.0014 0.0003 0.0063 0.0005 0.0148 0.0033 0.0037 0.0015 0.0249 0.0007 0.0326 0.0076 0.0134 0.0019 0.0005 0.0004
Nuclear Periphery 0.03 0.0123 0.0128 0.0215 0.0453 0.0102 0.0445 0.0222 0.0231 0.0411 0.0971 0.0081 0.0321 0.0106 0.0084 0.0033 0.0057 0.0043
Nucleolus 0.0268 0.028 0.0529 0.0173 0.0273 0.068 0.0295 0.0428 0.0423 0.0508 0.0278 0.075 0.0301 0.0496 0.06 0.0337 0.0139 0.0526
Nucleus 0.6865 0.9295 0.8492 0.8753 0.672 0.8702 0.6868 0.9045 0.8291 0.7958 0.5657 0.8751 0.5854 0.8929 0.8039 0.8292 0.9717 0.8479
Peroxisomes 0.0188 0.0015 0.0018 0.0015 0.0108 0.0027 0.0052 0 0.0025 0.0026 0.0092 0.0012 0.0067 0.0001 0.0093 0.0067 0 0.002
Punctate Nuclear 0.0534 0.0115 0.0225 0.0027 0.0188 0.0046 0.0452 0.0128 0.0134 0.0124 0.0959 0.0191 0.0174 0.0056 0.034 0.0418 0.0036 0.0095
Vacuole 0.0091 0.0005 0.0026 0.0005 0.0054 0.0012 0.0047 0.0017 0.0037 0.0138 0.0106 0.001 0.0099 0.0045 0.0012 0.0008 0.0004 0.0047
Vacuole Periphery 0.0035 0.0003 0.001 0.0007 0.0024 0.0008 0.0027 0.0005 0.001 0.0022 0.0064 0.0003 0.0039 0.0006 0.0005 0.0005 0.0002 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0021 5.7481 8.9572 9.0986 6.2527 10.2825 7.7008 7.2569 8.8046 12.4777
Translational Efficiency 1.1843 1.8132 0.9949 1.0499 1.6961 1.0959 0.7987 0.9668 0.8122 0.7547

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
185 146 192 218 2126 2080 1672 310 2311 2226 1864 528

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.40 825.38 1072.84 961.86 737.76 738.96 910.27 882.99 731.73 744.63 927.02 915.55
Standard Deviation 75.91 106.89 130.60 115.15 84.42 96.53 116.36 129.90 86.23 99.57 127.84 129.96
Intensity Change Log 2 0.317356 0.695660 0.538124 0.002345 0.303143 0.259246 0.159962 0.502173 0.397912

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000064 0.000355 0.001894 0.000925 0.000038 0.000229 0.000288 0.003509 0.000040 0.000237 0.000453 0.002442
Bud Neck 0.001416 0.002863 0.007382 0.017972 0.002915 0.009834 0.010246 0.010262 0.002795 0.009377 0.009951 0.013445
Bud Site 0.000573 0.000523 0.001829 0.032810 0.000492 0.002610 0.001703 0.022481 0.000499 0.002473 0.001716 0.026746
Cell Periphery 0.000201 0.000066 0.000128 0.000166 0.000106 0.000109 0.000115 0.000197 0.000114 0.000106 0.000116 0.000184
Cytoplasm 0.129104* 0.033375 0.007560 0.064337 0.085796 0.065381 0.033760 0.069087 0.089263 0.063282 0.031061 0.067126
Cytoplasmic Foci 0.023625 0.004360 0.000830 0.004782 0.007767 0.008580 0.000852 0.006633 0.009036 0.008303 0.000849 0.005869
Eisosomes 0.000051 0.000044 0.000072 0.000040 0.000018 0.000022 0.000018 0.000041 0.000021 0.000024 0.000023 0.000040
Endoplasmic Reticulum 0.001177 0.006056 0.020028 0.010171 0.001717 0.003682 0.004643 0.004577 0.001673 0.003838 0.006228 0.006887
Endosome 0.000913 0.000743 0.001044 0.017514 0.000969 0.002648 0.000737 0.011091 0.000964 0.002523 0.000769 0.013743
Golgi 0.000173 0.000092 0.000116 0.004143 0.000231 0.000993 0.000135 0.010982 0.000226 0.000934 0.000133 0.008158
Lipid Particles 0.003250 0.002368 0.001329 0.000698 0.002200 0.002162 0.000344 0.001595 0.002284 0.002175 0.000445 0.001224
Mitochondria 0.000835 0.000852 0.001257 0.006521 0.001645 0.008152 0.001718 0.008481 0.001580 0.007674 0.001671 0.007672
Mitotic Spindle 0.000151 0.000123 0.000163 0.063308 0.000807 0.009247 0.008721 0.039853 0.000754 0.008648 0.007840 0.049537
None 0.018518 0.004074 0.001377 0.011524 0.004806 0.004641 0.006405 0.016445 0.005904 0.004603 0.005887 0.014413
Nuclear Periphery 0.001620 0.002130 0.001744 0.003582 0.003928 0.005053 0.002647 0.001495 0.003743 0.004862 0.002554 0.002357
Nuclear Periphery Foci 0.000391 0.000906 0.000699 0.013895 0.000575 0.001014 0.000386 0.001081 0.000560 0.001007 0.000418 0.006372
Nucleolus 0.007239 0.009313 0.003319 0.003429 0.005225 0.006700 0.002761 0.002402 0.005386 0.006871 0.002818 0.002826
Nucleus 0.805733* 0.924488* 0.930882* 0.707251* 0.877909* 0.855518* 0.919401* 0.755998* 0.872131* 0.860041* 0.920584* 0.735871*
Peroxisomes 0.001802 0.000984 0.000419 0.000560 0.000620 0.002308 0.000193 0.002020 0.000715 0.002221 0.000216 0.001417
Vacuole 0.002832 0.006049 0.017668 0.032933 0.002044 0.009654 0.004604 0.023800 0.002107 0.009417 0.005950 0.027571
Vacuole Periphery 0.000331 0.000234 0.000261 0.003440 0.000194 0.001463 0.000323 0.007972 0.000205 0.001382 0.000316 0.006101

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.18 -13.58 -10.33 -6.96 2.60 -4.60 -15.84 -2.49 -2.23 -2.01 -5.11 -19.00 -3.80 -3.35 -2.63
Bud Neck -2.57 -9.50 -8.57 -7.47 -5.27 -7.08 -10.24 -6.86 -3.07 -1.25 -7.25 -10.91 -10.25 -5.32 -3.11
Bud Site -0.52 -5.81 -3.33 -3.30 -2.90 -5.41 -4.68 -3.21 -2.71 -2.83 -5.44 -5.26 -4.57 -3.91 -4.02
Cell Periphery 3.79 1.34 1.53 -3.72 0.44 -0.08 0.15 -1.35 -1.28 -1.36 0.58 0.31 -1.47 -1.94 -1.62
Cytoplasm 5.32 7.64 5.34 -0.41 -4.34 4.38 13.38 4.89 2.19 -2.87 5.74 15.31 7.68 3.44 -3.23
Cytoplasmic Foci 4.40 5.74 5.56 2.21 -0.78 -0.90 8.98 4.63 5.42 -2.01 0.48 10.47 7.36 7.01 -2.18
Eisosomes 0.38 -3.47 -3.36 -3.65 -0.29 -2.93 -3.04 -6.34 -5.35 -5.58 -2.21 -4.97 -9.56 -8.66 -7.88
Endoplasmic Reticulum -5.20 -14.24 -10.29 -5.56 3.06 -7.77 -16.05 -8.92 -6.28 -3.42 -8.90 -19.59 -13.12 -9.97 -5.02
Endosome -0.02 -1.18 -1.84 -1.85 -1.65 -3.91 0.73 -2.82 -1.50 -2.96 -3.91 0.46 -3.17 -1.87 -3.26
Golgi 0.62 0.42 -1.71 -1.85 -1.81 -2.74 0.98 -1.86 -1.48 -1.92 -2.73 1.01 -2.12 -1.51 -2.22
Lipid Particles 0.49 2.42 3.12 2.19 1.23 -0.25 6.98 2.77 2.85 -2.79 -0.12 7.23 3.92 3.72 -3.60
Mitochondria -0.60 -2.29 -2.35 -2.22 -1.88 -6.09 -0.87 -1.71 1.59 -1.53 -6.12 -0.95 -2.17 2.35 -1.91
Mitotic Spindle -0.94 -1.06 -4.04 -3.96 -4.03 -5.27 -5.37 -4.27 -3.05 -2.69 -5.31 -5.36 -5.83 -4.39 -4.04
None 2.32 2.98 1.76 -1.97 -5.47 0.32 -1.02 -3.06 -3.21 -2.52 1.64 0.69 -2.66 -3.88 -3.14
Nuclear Periphery -1.20 -0.93 -3.97 -2.61 -4.41 -1.95 0.97 1.45 2.82 0.28 -2.12 0.95 -0.30 1.85 -1.14
Nuclear Periphery Foci -2.00 -2.42 -1.68 -1.34 -1.46 -1.85 -0.33 -1.83 0.29 -1.10 -2.02 -0.49 -1.88 -1.21 -1.70
Nucleolus -1.51 2.22 0.86 2.40 -2.48 -1.94 2.30 -0.57 0.18 -1.34 -2.23 2.79 -0.39 0.89 -1.77
Nucleus -5.04 -5.39 2.25 7.61 8.19 3.56 -5.08 5.61 4.07 7.48 2.16 -6.46 7.96 6.76 10.91
Peroxisomes 0.78 1.61 1.71 2.11 0.98 -4.18 3.03 -0.38 3.19 -2.06 -3.95 3.40 0.62 4.25 -2.21
Vacuole 0.04 -3.29 -6.72 -7.33 -5.13 -7.58 -7.74 -6.71 -5.42 -5.79 -6.91 -8.39 -10.18 -8.84 -8.87
Vacuole Periphery 0.34 0.25 -1.66 -2.07 -2.03 -3.70 -0.80 -1.91 -1.01 -1.85 -3.67 -0.76 -2.28 -0.86 -2.18