Standard name | |
---|---|
Human Ortholog | |
Description | Transmembrane histidine phosphotransfer kinase and osmosensor; regulates MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 73 | 64 | 59 | 50 | 7 | 17 | 8 | 15 | 12 | 13 | 24 | 2 | 10 | 13 | 15 | 10 | 4 | 6 | 122 | 53 | 107 | 47 | 73 | 63 |
Bud | 17 | 24 | 13 | 19 | 12 | 34 | 37 | 44 | 45 | 35 | 33 | 28 | 1 | 1 | 1 | 3 | 3 | 4 | 4 | 2 | 3 | 1 | 2 | 3 |
Bud Neck | 8 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 4 | 0 | 47 | 24 | 17 | 15 | 29 | 37 |
Bud Site | 2 | 2 | 0 | 7 | 1 | 1 | 1 | 2 | 3 | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 153 | 72 | 153 | 92 | 66 | 108 | 98 | 80 | 85 | 74 | 56 | 69 | 24 | 55 | 52 | 30 | 19 | 19 | 320 | 153 | 266 | 78 | 167 | 153 |
Cytoplasm | 286 | 114 | 163 | 114 | 204 | 265 | 308 | 327 | 227 | 257 | 147 | 212 | 73 | 157 | 134 | 68 | 44 | 45 | 27 | 12 | 3 | 12 | 11 | 7 |
Endoplasmic Reticulum | 6 | 3 | 4 | 8 | 4 | 18 | 15 | 25 | 12 | 23 | 29 | 6 | 0 | 1 | 3 | 5 | 6 | 5 | 23 | 12 | 10 | 5 | 8 | 5 |
Endosome | 2 | 0 | 0 | 0 | 10 | 0 | 3 | 7 | 0 | 2 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 1 | 5 | 3 | 1 | 2 | 2 | 4 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 23 | 20 | 23 | 46 | 44 | 181 | 260 | 327 | 275 | 341 | 264 | 272 | 1 | 0 | 0 | 14 | 7 | 7 | 26 | 11 | 12 | 19 | 24 | 22 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 9 | 1 | 68 | 38 | 47 | 32 | 22 | 34 | 14 | 22 | 3 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
SpindlePole | 22 | 3 | 18 | 12 | 8 | 6 | 9 | 14 | 8 | 6 | 3 | 7 | 0 | 2 | 3 | 6 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 3 | 0 | 1 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 5 | 0 | 2 | 5 | 5 |
Unique Cell Count | 471 | 219 | 364 | 265 | 314 | 497 | 596 | 699 | 504 | 600 | 427 | 466 | 85 | 186 | 160 | 105 | 66 | 75 | 601 | 284 | 434 | 193 | 332 | 312 |
Labelled Cell Count | 604 | 304 | 506 | 390 | 406 | 662 | 763 | 885 | 682 | 781 | 563 | 621 | 112 | 232 | 210 | 141 | 87 | 96 | 601 | 284 | 434 | 193 | 332 | 312 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.5 | 5.3 | 5.1 | 5.0 | 4.2 | 4.0 | 4.2 | 3.9 | 3.7 | 3.7 | 3.7 | 6.0 | 6.3 | 6.4 | 4.9 | 5.7 | 6.4 | 6.2 | 6.3 | 6.7 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.5 | 1.7 | 1.7 | 1.1 | 1.0 | 0.9 | 0.8 | 0.8 | 0.6 | 0.6 | 1.0 | 0.9 | 0.7 | 1.5 | 1.7 | 1.6 | 1.4 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.09 | -0.33 | -0.39 | -0.33 | -0.43 | -0.53 | -0.52 | -0.53 | 0.17 | 0.24 | 0.27 | -0.11 | 0.11 | 0.28 | 0.22 | 0.24 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.9 | -6.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -3.0 | -2.1 | -1.7 | -2.1 | -1.8 |
Bud | 2.0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.9 | -5.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.3 | -2.9 | -2.1 | -2.5 | -2.0 | -2.7 |
Cytoplasm | -0.4 | 5.3 | 2.5 | 2.1 | 0.6 | 0.1 | -0.6 | -3.0 | 0.2 | 6.8 | 8.9 | 8.3 | 3.6 | 3.3 | 2.4 |
Endoplasmic Reticulum | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.5 | -3.3 | 2.3 | 0 | 0.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.4 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.3 | -6.3 | -5.9 | -4.8 | -3.8 | -4.1 |
SpindlePole | -0.2 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.3 | -1.7 | 0.3 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3937 | 1.7873 | 1.7293 | 1.4321 | 1.6498 | 1.6246 | 2.9327 | 3.575 | 3.3297 | 2.9751 | 3.2082 | 3.4134 | 2.525 | 3.4311 | 3.1233 | 3.916 | 2.993 | 3.2186 |
Actin | 0.084 | 0.0387 | 0.0476 | 0.0808 | 0.0344 | 0.0143 | 0.0907 | 0.0102 | 0.0399 | 0.0343 | 0.0429 | 0.0317 | 0.031 | 0.003 | 0.0201 | 0.0212 | 0.0038 | 0.035 |
Bud | 0.0031 | 0.0014 | 0.0012 | 0.0013 | 0.0015 | 0.0016 | 0.0019 | 0.0005 | 0.0177 | 0.002 | 0.0008 | 0.0016 | 0.0003 | 0.0003 | 0.0008 | 0.0006 | 0.0012 | 0.0008 |
Bud Neck | 0.0141 | 0.0097 | 0.0776 | 0.0576 | 0.0592 | 0.0353 | 0.0165 | 0.0088 | 0.053 | 0.0707 | 0.0971 | 0.0512 | 0.0053 | 0.0065 | 0.0571 | 0.0888 | 0.0427 | 0.0499 |
Bud Periphery | 0.018 | 0.0031 | 0.0061 | 0.0077 | 0.0103 | 0.0159 | 0.0067 | 0.0022 | 0.0127 | 0.0158 | 0.0112 | 0.0113 | 0.0016 | 0.0016 | 0.0067 | 0.005 | 0.0071 | 0.0099 |
Bud Site | 0.0467 | 0.1931 | 0.1112 | 0.0164 | 0.0092 | 0.0028 | 0.1023 | 0.2273 | 0.1173 | 0.0194 | 0.0058 | 0.0067 | 0.0273 | 0.1488 | 0.0789 | 0.0203 | 0.0105 | 0.0049 |
Cell Periphery | 0.2019 | 0.2057 | 0.2407 | 0.2697 | 0.224 | 0.278 | 0.2338 | 0.1986 | 0.2231 | 0.3304 | 0.3138 | 0.255 | 0.2315 | 0.2571 | 0.2845 | 0.2716 | 0.3544 | 0.3162 |
Cytoplasm | 0.3569 | 0.4448 | 0.3777 | 0.4994 | 0.3194 | 0.5326 | 0.346 | 0.4111 | 0.2872 | 0.4244 | 0.4111 | 0.4957 | 0.4957 | 0.4743 | 0.3481 | 0.4866 | 0.3586 | 0.4557 |
Cytoplasmic Foci | 0.0337 | 0.0098 | 0.0149 | 0.0027 | 0.0193 | 0.0028 | 0.0302 | 0.0047 | 0.0201 | 0.0024 | 0.0283 | 0.0063 | 0.0448 | 0.0011 | 0.0098 | 0.0575 | 0.0126 | 0.0045 |
Eisosomes | 0.0291 | 0.0076 | 0.0068 | 0.0036 | 0.0167 | 0.0036 | 0.0103 | 0.0034 | 0.017 | 0.0125 | 0.0055 | 0.0036 | 0.0063 | 0.0031 | 0.006 | 0.0017 | 0.0028 | 0.0037 |
Endoplasmic Reticulum | 0.0076 | 0.0039 | 0.004 | 0.0038 | 0.004 | 0.0032 | 0.0034 | 0.0024 | 0.0039 | 0.0033 | 0.0025 | 0.0082 | 0.0014 | 0.0017 | 0.007 | 0.0025 | 0.0024 | 0.004 |
Endosome | 0.0221 | 0.002 | 0.0045 | 0.0006 | 0.0277 | 0.0013 | 0.008 | 0.0016 | 0.0035 | 0.0003 | 0.0085 | 0.0028 | 0.0011 | 0.0001 | 0.0175 | 0.002 | 0.0003 | 0.0052 |
Golgi | 0.015 | 0.0048 | 0.0063 | 0.0026 | 0.0264 | 0.0014 | 0.0106 | 0.0005 | 0.0067 | 0.0006 | 0.0029 | 0.0025 | 0.0033 | 0.0003 | 0.0181 | 0.0025 | 0.0004 | 0.0073 |
Lipid Particles | 0.0178 | 0.0023 | 0.0035 | 0.0013 | 0.0027 | 0.0012 | 0.0106 | 0.005 | 0.0115 | 0.0006 | 0.0036 | 0.0033 | 0.0064 | 0.0003 | 0.0102 | 0.0059 | 0.0012 | 0.0104 |
Mitochondria | 0.01 | 0.0008 | 0.0115 | 0.0003 | 0.0136 | 0.001 | 0.0046 | 0.0002 | 0.0055 | 0.0001 | 0.0002 | 0.0014 | 0.0009 | 0 | 0.0118 | 0.0004 | 0.0004 | 0.0026 |
None | 0.0958 | 0.0693 | 0.0792 | 0.0494 | 0.2244 | 0.1024 | 0.1031 | 0.1133 | 0.143 | 0.0807 | 0.0406 | 0.1119 | 0.0032 | 0.1006 | 0.1152 | 0.0141 | 0.1946 | 0.0817 |
Nuclear Periphery | 0.0025 | 0.0003 | 0.0007 | 0.0003 | 0.0004 | 0.0003 | 0.0011 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0006 |
Nucleolus | 0.0016 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0.0056 | 0.001 | 0.0002 | 0.0004 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0001 |
Nucleus | 0.0021 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0008 | 0.0008 | 0.0019 | 0.0004 | 0.0008 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0008 | 0.0003 |
Peroxisomes | 0.026 | 0.0006 | 0.0034 | 0.0006 | 0.0028 | 0.0002 | 0.0079 | 0.0006 | 0.0306 | 0.0001 | 0.0029 | 0.0008 | 0.139 | 0 | 0.0038 | 0.0174 | 0.0013 | 0.0049 |
Punctate Nuclear | 0.0054 | 0.0004 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0065 | 0.0004 | 0.0024 | 0.0001 | 0.0194 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0005 | 0.0021 | 0.0005 |
Vacuole | 0.0055 | 0.0011 | 0.0015 | 0.0014 | 0.0018 | 0.0014 | 0.0037 | 0.0026 | 0.0016 | 0.0016 | 0.0009 | 0.0035 | 0.0003 | 0.0006 | 0.0021 | 0.0007 | 0.0019 | 0.0015 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.1056 | 29.3516 | 24.3778 | 25.4797 | 30.854 | 31.6116 | 29.269 | 29.4022 | 26.7146 | 41.9269 |
Translational Efficiency | 0.5778 | 0.3562 | 0.3629 | 0.3411 | 0.3665 | 0.5757 | 0.4028 | 0.2937 | 0.3639 | 0.3142 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2233 | 804 | 259 | 1468 | 1538 | 1323 | 173 | 2055 | 3771 | 2127 | 432 | 3523 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 781.13 | 800.48 | 938.27 | 895.92 | 578.87 | 783.68 | 933.87 | 864.94 | 698.64 | 790.03 | 936.51 | 877.85 |
Standard Deviation | 86.68 | 101.54 | 100.81 | 122.05 | 69.37 | 100.51 | 89.08 | 108.04 | 127.64 | 101.23 | 96.31 | 115.10 |
Intensity Change Log 2 | — | 0.035303 | 0.264440 | 0.197807 | — | 0.437025 | 0.689982 | 0.579361 | — | 0.220111 | 0.461082 | 0.372674 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.036920 | 0.052271 | 0.049655 | 0.111762* | 0.024814 | 0.021061 | 0.039493 | 0.056066 | 0.031983 | 0.032858 | 0.045586 | 0.079274 |
Bud Neck | 0.064940 | 0.085523 | 0.037406 | 0.057139 | 0.058926 | 0.088624 | 0.045245 | 0.064736 | 0.062487 | 0.087452 | 0.040545 | 0.061570 |
Bud Site | 0.056068 | 0.068587 | 0.098999 | 0.137740* | 0.035763 | 0.033599 | 0.113316* | 0.100623* | 0.047787 | 0.046824 | 0.104733* | 0.116089* |
Cell Periphery | 0.053889 | 0.098812 | 0.076280 | 0.154728* | 0.068433 | 0.079478 | 0.087208 | 0.087445 | 0.059821 | 0.086786 | 0.080656 | 0.115481* |
Cytoplasm | 0.415572* | 0.422909* | 0.225446* | 0.199965* | 0.390694* | 0.454791* | 0.236439* | 0.372199* | 0.405426* | 0.442740* | 0.229848* | 0.300431* |
Cytoplasmic Foci | 0.025655 | 0.050570 | 0.126375 | 0.010074 | 0.064530 | 0.055436 | 0.059657 | 0.037765 | 0.041510 | 0.053596 | 0.099657 | 0.026226 |
Eisosomes | 0.088693 | 0.111848* | 0.031472 | 0.132708* | 0.092709 | 0.122920* | 0.042986 | 0.098011 | 0.090331 | 0.118735* | 0.036083 | 0.112469* |
Endoplasmic Reticulum | 0.004803 | 0.004702 | 0.053368 | 0.013646 | 0.010412 | 0.003713 | 0.044179 | 0.004310 | 0.007090 | 0.004086 | 0.049689 | 0.008200 |
Endosome | 0.001709 | 0.006638 | 0.007790 | 0.002100 | 0.007099 | 0.009259 | 0.003019 | 0.003086 | 0.003907 | 0.008268 | 0.005880 | 0.002675 |
Golgi | 0.002878 | 0.022500 | 0.004559 | 0.001564 | 0.039455 | 0.023698 | 0.002430 | 0.003282 | 0.017796 | 0.023245 | 0.003707 | 0.002566 |
Lipid Particles | 0.004514 | 0.009153 | 0.016972 | 0.003180 | 0.037217 | 0.018999 | 0.009601 | 0.008086 | 0.017852 | 0.015277 | 0.014020 | 0.006042 |
Mitochondria | 0.002133 | 0.007813 | 0.000329 | 0.001195 | 0.058240 | 0.013278 | 0.000358 | 0.001203 | 0.025016 | 0.011212 | 0.000341 | 0.001200 |
Mitotic Spindle | 0.001132 | 0.000097 | 0.000224 | 0.000425 | 0.000121 | 0.000359 | 0.000266 | 0.001391 | 0.000720 | 0.000260 | 0.000241 | 0.000989 |
None | 0.000597 | 0.000618 | 0.000251 | 0.000452 | 0.004120 | 0.000582 | 0.000201 | 0.000725 | 0.002034 | 0.000596 | 0.000231 | 0.000611 |
Nuclear Periphery | 0.000101 | 0.000062 | 0.000467 | 0.000097 | 0.000100 | 0.000121 | 0.000365 | 0.000132 | 0.000101 | 0.000099 | 0.000426 | 0.000118 |
Nuclear Periphery Foci | 0.000410 | 0.000330 | 0.006132 | 0.000848 | 0.000361 | 0.000239 | 0.003420 | 0.000612 | 0.000390 | 0.000273 | 0.005046 | 0.000710 |
Nucleolus | 0.000098 | 0.000086 | 0.000222 | 0.000055 | 0.000254 | 0.000309 | 0.000091 | 0.000149 | 0.000161 | 0.000225 | 0.000169 | 0.000110 |
Nucleus | 0.174253* | 0.017867 | 0.135756* | 0.085020 | 0.013049 | 0.026479 | 0.192396* | 0.086889 | 0.108506 | 0.023224 | 0.158438* | 0.086110 |
Peroxisomes | 0.004464 | 0.019240 | 0.004895 | 0.001721 | 0.086301 | 0.025664 | 0.002623 | 0.004123 | 0.037841 | 0.023236 | 0.003985 | 0.003122 |
Vacuole | 0.061057 | 0.019787 | 0.123215 | 0.084733 | 0.006116 | 0.020231 | 0.116638 | 0.068716 | 0.038649 | 0.020063 | 0.120581 | 0.075390 |
Vacuole Periphery | 0.000114 | 0.000588 | 0.000186 | 0.000848 | 0.001285 | 0.001160 | 0.000069 | 0.000451 | 0.000592 | 0.000944 | 0.000139 | 0.000617 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.85 | -1.79 | -15.57 | -10.40 | -7.99 | 1.71 | -2.22 | -10.36 | -12.07 | -2.55 | -0.47 | -2.72 | -17.21 | -15.91 | -6.58 |
Bud Neck | -3.92 | 5.60 | 2.57 | 5.53 | -3.88 | -6.22 | 2.16 | -1.36 | 5.51 | -3.00 | -7.23 | 5.57 | 0.61 | 7.83 | -5.27 |
Bud Site | -2.64 | -4.32 | -13.80 | -10.41 | -3.76 | 0.72 | -5.76 | -13.71 | -14.01 | 0.91 | 0.30 | -7.11 | -17.99 | -17.67 | -1.44 |
Cell Periphery | -7.51 | -2.44 | -18.60 | -6.97 | -8.32 | -1.96 | -1.50 | -3.62 | -1.35 | -0.26 | -6.98 | -2.81 | -15.69 | -6.07 | -5.30 |
Cytoplasm | -0.84 | 16.06 | 30.12 | 22.45 | 1.92 | -7.36 | 11.03 | 2.38 | 9.78 | -9.81 | -5.96 | 19.43 | 19.00 | 21.59 | -7.80 |
Cytoplasmic Foci | -7.22 | -10.93 | 10.50 | 12.20 | 12.66 | 2.53 | 0.78 | 8.17 | 5.07 | 3.57 | -4.99 | -9.33 | 8.28 | 11.19 | 11.69 |
Eisosomes | -3.90 | 11.91 | -7.80 | -2.50 | -17.13 | -5.39 | 7.16 | -0.91 | 4.65 | -7.73 | -6.98 | 13.49 | -5.94 | 1.94 | -17.46 |
Endoplasmic Reticulum | 0.14 | -9.02 | -8.93 | -8.47 | 7.19 | 6.56 | -5.35 | 5.94 | -1.44 | 6.38 | 5.47 | -10.38 | -2.05 | -8.19 | 10.07 |
Endosome | -6.06 | -6.72 | -0.80 | 5.09 | 5.86 | -2.13 | 5.86 | 6.19 | 6.92 | -0.25 | -6.62 | -3.22 | 3.39 | 8.59 | 5.22 |
Golgi | -13.59 | -2.37 | 3.27 | 14.44 | 4.26 | 8.36 | 24.25 | 23.89 | 16.64 | -1.67 | -4.79 | 17.74 | 20.92 | 22.05 | 2.26 |
Lipid Particles | -5.78 | -9.06 | 2.44 | 7.01 | 9.79 | 8.31 | 13.60 | 14.94 | 7.61 | 1.35 | 2.23 | 3.00 | 12.73 | 9.75 | 7.92 |
Mitochondria | -6.59 | 4.38 | 1.74 | 7.84 | -2.24 | 15.38 | 22.00 | 21.64 | 9.29 | -3.27 | 9.45 | 20.76 | 19.81 | 11.48 | -4.01 |
Mitotic Spindle | 2.39 | 2.09 | 1.45 | -2.17 | -1.39 | -1.18 | -1.05 | -3.45 | -2.44 | -2.90 | 1.57 | 1.82 | -0.89 | -2.85 | -3.29 |
None | -0.09 | 3.52 | 0.87 | 0.72 | -1.13 | 4.69 | 5.21 | 4.40 | -0.71 | -2.82 | 4.48 | 5.75 | 4.27 | -0.09 | -2.86 |
Nuclear Periphery | 4.82 | -7.37 | 0.14 | -4.03 | 7.35 | -1.05 | -6.59 | -2.06 | -0.61 | 5.64 | 0.18 | -9.59 | -2.08 | -1.56 | 8.87 |
Nuclear Periphery Foci | 1.62 | -11.57 | -6.16 | -6.77 | 10.46 | 0.79 | -8.61 | -1.75 | -6.33 | 8.47 | 1.72 | -13.88 | -4.58 | -9.36 | 12.91 |
Nucleolus | 0.72 | -3.63 | 1.47 | 1.06 | 4.14 | -0.31 | 2.20 | 1.26 | 0.98 | -1.51 | -0.61 | -0.20 | 1.28 | 1.12 | 1.86 |
Nucleus | 38.25* | 3.68 | 16.79 | -18.70 | 5.38 | -7.95 | -13.34 | -27.65 | -20.57 | 7.76 | 29.74 | -6.33 | 6.43 | -27.48 | 9.19 |
Peroxisomes | -10.94 | -0.52 | 6.67 | 13.38 | 4.65 | 17.39* | 26.84* | 26.40* | 12.99 | -2.75 | 8.02 | 22.60 | 23.86 | 17.59 | 1.68 |
Vacuole | 14.98 | -7.67 | -6.82 | -16.86 | 3.65 | -9.47 | -11.98 | -26.48 | -18.75 | 5.16 | 10.86 | -13.39 | -15.58 | -24.93 | 6.79 |
Vacuole Periphery | -6.23 | -2.34 | -2.04 | -0.66 | -1.82 | 0.49 | 14.47 | 4.45 | 2.43 | -2.29 | -2.26 | 11.24 | -0.09 | 1.46 | -2.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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