Standard name
Description Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0.20 0.06 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0.12 0.17 0.08 0.11 0.09 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.51 0.52 0.34 0.27 0.37 0.39 0.40 0.35 0.37 0.39 0.35 0.44 0.56 0.45 0.44 0.45 0.47 0.48 0 0.06 0 0 0 0
Peroxisomes 0.05 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0
SpindlePole 0.89 0.85 0.93 0.91 0.88 0.87 0.82 0.87 0.87 0.86 0.78 0.84 0.83 0.73 0.65 0.81 0.80 0.83 0.92 0.84 0.89 0.87 0.82 0.85
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 2 0 0 1 0
Bud 7 4 2 3 4 3 5 18 11 12 9 5 0 0 0 9 4 3 2 4 1 2 2 7
Bud Neck 8 6 11 4 8 12 8 10 11 15 11 5 1 3 3 3 4 2 0 5 3 0 0 3
Bud Site 11 3 2 3 1 9 13 12 15 20 12 8 3 1 1 10 5 9
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 6 13 12 7 14 13 13 17 10 11 9 8 17 45 80 35 29 26 0 1 0 1 2 3
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 5 4 2 0 0 1 0 0 1
Endosome 16 44 9 7 10 13 8 10 12 6 7 15 7 41 69 45 44 31 16 18 9 9 19 10
Golgi 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1 0 2 3 4 2 0 1 0
Mitochondria 16 5 0 3 1 5 8 4 8 12 29 7 2 0 1 30 13 11 2 3 0 0 4 3
Nucleus 14 14 4 8 16 12 16 19 10 11 12 11 4 5 15 29 30 30 0 1 0 2 4 3
Nuclear Periphery 0 1 0 1 0 0 1 0 2 0 0 1 0 0 0 0 1 3 0 1 0 0 1 1
Nucleolus 300 302 136 68 137 159 135 190 149 185 112 154 120 149 180 245 183 170 10 34 13 3 12 7
Peroxisomes 28 32 5 3 5 11 3 9 16 8 16 9 0 8 30 19 14 6 5 5 3 3 1 3
SpindlePole 519 495 371 231 326 357 280 468 354 405 250 293 177 243 266 435 313 292 554 505 365 192 270 296
Vac/Vac Membrane 0 5 1 1 0 0 0 1 1 0 0 0 1 2 3 10 4 4 0 1 0 1 2 3
Unique Cell Count 586 579 398 254 371 410 341 541 408 472 319 348 214 332 410 539 391 353 604 603 411 222 329 350
Labelled Cell Count 926 924 554 340 522 594 490 758 599 686 468 517 332 497 650 876 648 591 604 603 411 222 329 350

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

SpindlePole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.7 7.1 6.5 7.0 6.1 6.3 6.5 5.6 5.9 5.3 5.7 7.1 7.1 6.9 7.5 7.7 7.1 6.7 6.8 6.8
Std Deviation (1e-4) 1.0 1.3 1.1 1.3 1.6 1.6 1.5 1.4 1.4 1.6 1.8 1.3 1.3 1.6 1.7 1.7 1.7 1.6 1.4 1.5 1.4
Intensity Change (Log2) -0.1 -0.0 -0.2 -0.2 -0.1 -0.3 -0.3 -0.4 -0.3 0.0 -0.0 -0.0 0.1 0.1 -0.0 -0.1 -0.1 -0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 3.0 2.5 2.4 0 0 0 0 0 0 0 0
Bud Neck -1.0 -0.5 0.1 -0.4 -0.9 -0.1 0.4 0.5 -1.2 0 -1.8 -2.2 -2.8 -1.8 -2.3
Bud Site 0 0 2.1 3.2 2.1 3.1 3.5 3.1 0 0 0 0 1.8 0 2.3
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 1.5 1.3 2.1 2.1 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.4 0.4 0.8 0.1 -0.4 0.6 -1.1 -0.1 1.6 0.7 5.4 7.0 4.0 5.0 4.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.2 6.1 0 0 0 0 4.8 3.7 3.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 0.8 1.4 1.5 0.3 0.7 1.5 0.3 2.8 5.2 3.0 2.8 3.5 3.6 3.9
Peroxisomes 0 0 1.5 0 0.5 2.4 0.5 3.0 1.3 0 1.2 4.2 2.2 2.1 0.5
SpindlePole -1.1 -2.5 -2.9 -4.6 -3.3 -3.0 -3.5 -5.8 -3.9 -4.1 -7.4 -9.9 -5.5 -5.4 -4.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0