Standard name | |
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Human Ortholog | |
Description | Component of RNA polymerase transcription factor TFIIH holoenzyme; acts as dsDNA-dependent translocase in context of TFIIH, unwinds DNA strands during initiation and promotes transcription start site (TSS) scanning; has DNA-dependent ATPase/helicase activity; interacts functionally with TFIIB, has roles in TSS selection and gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to cytosol under hypoxia; homolog of human ERCC3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | – | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | – | 8 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 2 | 8 | 7 | 18 | 13 | 16 | 17 | 15 | – | 13 | – | 11 | 38 | 57 | 33 | 19 | 17 | 0 | 0 | 1 | 2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 3 | 6 | 7 | 0 | 0 | 0 | 1 | 0 | 1 |
Endosome | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 4 | 2 | – | 0 | – | 1 | 0 | 2 | 0 | 4 | 3 | 2 | 0 | 1 | 1 | 2 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 3 |
Mitochondria | 11 | 12 | 2 | 21 | 4 | 42 | 63 | 50 | 95 | – | 132 | – | 10 | 1 | 1 | 3 | 0 | 3 | 1 | 0 | 3 | 1 | 0 | 4 |
Nucleus | 93 | 165 | 286 | 170 | 287 | 311 | 545 | 547 | 384 | – | 297 | – | 396 | 540 | 518 | 279 | 179 | 181 | 85 | 177 | 288 | 196 | 412 | 418 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 9 | 3 | – | 3 | – | 1 | 3 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 20 | 27 | 1 | 12 | 3 | 8 | 17 | 27 | 25 | – | 22 | – | 26 | 36 | 37 | 11 | 8 | 3 | 8 | 7 | 8 | 12 | 53 | 69 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | – | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 2 | 2 | 2 | 3 | 14 | 6 | – | 8 | – | 2 | 1 | 4 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 3 | 10 |
Vac/Vac Membrane | 0 | 3 | 5 | 2 | 2 | 1 | 2 | 5 | 5 | – | 8 | – | 0 | 1 | 5 | 12 | 9 | 12 | 1 | 6 | 11 | 11 | 37 | 56 |
Unique Cell Count | 109 | 179 | 302 | 186 | 312 | 345 | 587 | 606 | 448 | 373 | 419 | 595 | 593 | 329 | 221 | 215 | 108 | 196 | 322 | 235 | 522 | 596 | ||
Labelled Cell Count | 133 | 209 | 304 | 215 | 321 | 380 | 657 | 675 | 538 | 491 | 447 | 625 | 628 | 343 | 232 | 230 | 108 | 196 | 322 | 235 | 522 | 596 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 7.1 | 7.1 | 6.1 | 7.7 | 6.1 | 6.0 | 6.2 | 5.3 | – | 4.7 | – | 7.9 | 8.1 | 7.5 | 10.4 | 10.2 | 10.3 | 6.8 | 6.5 | 7.2 |
Std Deviation (1e-4) | 1.2 | 1.3 | 1.2 | 1.7 | 1.7 | 1.5 | 1.5 | 1.7 | 1.4 | – | 1.2 | – | 1.6 | 1.8 | 1.9 | 2.3 | 2.5 | 2.5 | 1.5 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.23 | 0.11 | -0.24 | -0.25 | -0.2 | -0.44 | – | -0.61 | – | 0.15 | 0.18 | 0.06 | 0.54 | 0.51 | 0.53 | -0.08 | -0.13 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 1.9 | 0.8 | 0.1 | 0.1 | 0.5 | – | 0.6 | – | 0 | 2.4 | 3.8 | 3.8 | 3.0 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.4 | 0 | 5.8 | 5.5 | 4.6 | 8.2 | – | 0 | – | 1.8 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.4 | -1.3 | -2.2 | -1.1 | -2.3 | -3.9 | – | -5.7 | – | -0.1 | -2.1 | -3.4 | -4.1 | -4.9 | -4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.6 | 3.4 | 3.9 | – | 0 | – | 4.1 | 4.1 | 4.1 | 2.8 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0.5 | – | 0 | 0 | 0 | 1.5 | 1.7 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5666 | 5.2522 | 4.629 | 4.5921 | 4.4447 | 4.6634 | 2.2545 | 4.2324 | 3.187 | 2.7655 | 2.1087 | 2.6699 | 3.938 | 5.111 | 4.4262 | 4.3994 | 3.6427 | 4.0183 |
Actin | 0.0308 | 0 | 0.0036 | 0 | 0.0329 | 0.0107 | 0.0118 | 0 | 0.0017 | 0 | 0.0002 | 0.0016 | 0.013 | 0 | 0.0077 | 0.0071 | 0.0046 | 0.0001 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0017 | 0.0012 | 0 |
Bud Neck | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0023 | 0.0009 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0003 | 0 | 0.0003 | 0.0009 | 0.0017 | 0.0007 |
Bud Periphery | 0.0002 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0013 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0025 | 0.0022 | 0 |
Bud Site | 0.0003 | 0 | 0.0008 | 0 | 0.002 | 0.0001 | 0.0035 | 0 | 0.0002 | 0 | 0.001 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0022 | 0.0012 | 0.0001 |
Cell Periphery | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 |
Cytoplasm | 0.0006 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0014 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0032 | 0.0043 | 0.0005 |
Cytoplasmic Foci | 0.0032 | 0 | 0.0025 | 0 | 0.0041 | 0.0002 | 0.024 | 0 | 0.0053 | 0 | 0.0127 | 0.0007 | 0.004 | 0 | 0.0012 | 0.0031 | 0.0048 | 0.0061 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0003 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0006 | 0 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0033 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.002 | 0 | 0.0001 | 0.0021 | 0.0017 | 0.0001 |
Endosome | 0.0025 | 0 | 0.0008 | 0 | 0.0013 | 0.0009 | 0.0287 | 0 | 0.008 | 0 | 0.0048 | 0.0013 | 0.0087 | 0 | 0.002 | 0.0011 | 0.0237 | 0.0005 |
Golgi | 0.0022 | 0 | 0.0005 | 0 | 0.0028 | 0.0019 | 0.008 | 0 | 0.0041 | 0 | 0.002 | 0.0008 | 0.0038 | 0 | 0.0004 | 0.0003 | 0.0058 | 0.0001 |
Lipid Particles | 0.0078 | 0 | 0.0028 | 0 | 0.0084 | 0.0013 | 0.0269 | 0 | 0.006 | 0 | 0.022 | 0.0022 | 0.008 | 0 | 0.0005 | 0.0012 | 0.0105 | 0.0015 |
Mitochondria | 0.0009 | 0.0001 | 0.0029 | 0 | 0.0022 | 0.0003 | 0.007 | 0.0001 | 0.0052 | 0.0002 | 0.034 | 0.0054 | 0.0158 | 0 | 0.0017 | 0.0038 | 0.0509 | 0.0002 |
None | 0.0008 | 0 | 0.0015 | 0 | 0.0003 | 0.0001 | 0.0014 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.002 | 0 | 0.0002 | 0.0095 | 0.0108 | 0.0024 |
Nuclear Periphery | 0.0048 | 0.0002 | 0.0015 | 0.0002 | 0.0012 | 0.0003 | 0.0125 | 0.0006 | 0.0005 | 0.0005 | 0.0036 | 0.0005 | 0.0071 | 0.0025 | 0.0006 | 0.0024 | 0.012 | 0.0009 |
Nucleolus | 0.026 | 0.0212 | 0.0129 | 0.0039 | 0.0926 | 0.0602 | 0.0202 | 0.0097 | 0.0109 | 0.0169 | 0.1092 | 0.0688 | 0.0254 | 0.0081 | 0.0132 | 0.0126 | 0.1419 | 0.0919 |
Nucleus | 0.9056 | 0.9781 | 0.9503 | 0.9957 | 0.8282 | 0.9218 | 0.8214 | 0.9893 | 0.9495 | 0.9821 | 0.769 | 0.9153 | 0.8946 | 0.9852 | 0.9621 | 0.9402 | 0.6989 | 0.8883 |
Peroxisomes | 0.0051 | 0 | 0.0054 | 0 | 0.0151 | 0.0002 | 0.0138 | 0 | 0.0057 | 0 | 0.0255 | 0.0007 | 0.002 | 0 | 0.004 | 0.0006 | 0.0009 | 0.0001 |
Punctate Nuclear | 0.0072 | 0.0001 | 0.0109 | 0.0001 | 0.0038 | 0.0002 | 0.0049 | 0 | 0.0003 | 0.0002 | 0.007 | 0.0002 | 0.0066 | 0.004 | 0.001 | 0.0034 | 0.0185 | 0.0062 |
Vacuole | 0.0003 | 0 | 0.0009 | 0 | 0.0006 | 0.0001 | 0.0054 | 0.0001 | 0.001 | 0 | 0.0053 | 0.0006 | 0.0014 | 0 | 0.0026 | 0.0011 | 0.002 | 0.0001 |
Vacuole Periphery | 0.0002 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0026 | 0.0001 | 0.0004 | 0 | 0.0014 | 0.0003 | 0.0025 | 0 | 0.002 | 0.0005 | 0.002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.4818 | 22.5947 | 23.704 | 25.879 | 19.7754 | 21.3416 | 23.3379 | 26.8506 | 26.1582 | 22.7977 |
Translational Efficiency | 1.0566 | 1.059 | 0.9025 | 0.8723 | 0.8861 | 0.9899 | 0.9574 | 0.7558 | 0.7687 | 0.7786 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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