Standard name | |
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Human Ortholog | |
Description | Eisosome component; likely plays only a minor role in eisosome assembly; shown to interact with Seg1p by affinity purification and mass spec; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; similar to <i>A. gossypii</i> SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 61 | 62 | 48 | 3 | 15 | 8 | 0 | 7 | 0 | – | 0 | 0 | 20 | 36 | 27 | 63 | 58 | 37 | 115 | 121 | 171 | 35 | 58 | 73 |
Bud | 6 | 10 | 26 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 6 | 10 | 15 | 9 | 8 | 9 | 0 | 0 | 2 | 1 | 1 | 1 |
Bud Neck | 1 | 15 | 42 | 13 | 16 | 19 | 3 | 3 | 0 | – | 0 | 2 | 2 | 6 | 6 | 0 | 7 | 0 | 5 | 24 | 25 | 10 | 11 | 21 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 25 | 42 | 26 | 13 | 15 | 21 | 10 | 11 | 7 | – | 14 | 11 | 27 | 51 | 75 | 61 | 49 | 47 | 85 | 134 | 192 | 68 | 85 | 77 |
Cytoplasm | 101 | 197 | 362 | 178 | 183 | 203 | 217 | 253 | 238 | – | 179 | 202 | 90 | 218 | 251 | 63 | 62 | 53 | 11 | 27 | 23 | 10 | 13 | 9 |
Endoplasmic Reticulum | 1 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 13 | 13 | 12 | 6 | 7 | 14 | 1 | 3 | 0 |
Endosome | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 5 | 8 | 0 | 1 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 3 | 1 | 0 | 1 | 1 |
Mitochondria | 5 | 10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 13 | 10 | 4 | 6 | 16 | 44 | 7 | 16 | 10 |
Nucleus | 2 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 5 | 3 | 8 | 0 | 2 | 2 |
SpindlePole | 21 | 5 | 17 | 38 | 85 | 60 | 55 | 37 | 49 | – | 34 | 41 | 1 | 9 | 5 | 21 | 29 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 6 | 5 | 19 | 3 | 4 | – | 1 | 1 | 1 | 0 | 0 | 2 | 4 | 1 | 1 | 7 | 4 | 2 | 1 | 0 |
Unique Cell Count | 190 | 293 | 447 | 207 | 261 | 262 | 273 | 285 | 267 | 199 | 224 | 121 | 260 | 290 | 190 | 172 | 141 | 253 | 363 | 515 | 143 | 202 | 207 | |
Labelled Cell Count | 235 | 352 | 529 | 245 | 321 | 316 | 307 | 316 | 300 | 229 | 258 | 148 | 333 | 383 | 251 | 247 | 180 | 253 | 363 | 515 | 143 | 202 | 207 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 4.3 | 3.8 | 7.2 | 7.8 | 7.2 | 7.1 | 7.1 | 7.7 | – | 8.6 | 8.6 | 4.7 | 5.0 | 4.5 | 7.0 | 7.5 | 7.4 | 4.8 | 5.2 | 5.4 |
Std Deviation (1e-4) | 1.1 | 0.7 | 0.6 | 0.7 | 5.8 | 0.8 | 0.8 | 0.9 | 0.8 | – | 0.6 | 0.7 | 0.9 | 1.0 | 0.9 | 1.4 | 1.7 | 1.9 | 1.3 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.94 | 1.06 | 0.95 | 0.93 | 0.93 | 1.04 | – | 1.19 | 1.2 | 0.31 | 0.42 | 0.26 | 0.91 | 1.0 | 0.97 | 0.34 | 0.46 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2948 | 1.897 | 1.8104 | 1.3309 | 1.8657 | 1.8217 | -0.1674 | -0.7512 | -0.698 | -1.0385 | -0.2431 | -0.7211 | 4.3916 | 3.9567 | 3.9174 | 4.2882 | 3.7445 | 4.2216 |
Actin | 0.0834 | 0.0288 | 0.0609 | 0.0349 | 0.0222 | 0.0248 | 0.0571 | 0.0483 | 0.0588 | 0.1159 | 0.1139 | 0.0326 | 0.0681 | 0.1209 | 0.0466 | 0.1084 | 0.0474 | 0.0216 |
Bud | 0.0077 | 0.0011 | 0.005 | 0.001 | 0.0022 | 0.0033 | 0.0096 | 0.0084 | 0.0061 | 0.0233 | 0.0009 | 0.0065 | 0.0012 | 0.0004 | 0.0064 | 0.0008 | 0.0016 | 0.0021 |
Bud Neck | 0.0234 | 0.0056 | 0.0419 | 0.0477 | 0.0249 | 0.0422 | 0.0103 | 0.011 | 0.0244 | 0.018 | 0.0254 | 0.0298 | 0.0125 | 0.0118 | 0.0485 | 0.0188 | 0.0373 | 0.0227 |
Bud Periphery | 0.0214 | 0.0035 | 0.0187 | 0.0052 | 0.0085 | 0.0207 | 0.0165 | 0.0091 | 0.012 | 0.0435 | 0.0037 | 0.0166 | 0.0054 | 0.0016 | 0.0176 | 0.0058 | 0.0083 | 0.0154 |
Bud Site | 0.0482 | 0.1407 | 0.0977 | 0.0117 | 0.0075 | 0.0066 | 0.0548 | 0.1734 | 0.0861 | 0.0211 | 0.0042 | 0.0051 | 0.0503 | 0.1295 | 0.084 | 0.0042 | 0.009 | 0.0065 |
Cell Periphery | 0.0706 | 0.0623 | 0.0849 | 0.0381 | 0.0532 | 0.0536 | 0.0875 | 0.0386 | 0.0578 | 0.0406 | 0.0292 | 0.041 | 0.1036 | 0.0826 | 0.0804 | 0.0558 | 0.0666 | 0.0725 |
Cytoplasm | 0.3365 | 0.4567 | 0.4409 | 0.4583 | 0.4574 | 0.5382 | 0.2803 | 0.333 | 0.3998 | 0.4188 | 0.4199 | 0.4171 | 0.417 | 0.4928 | 0.4117 | 0.4394 | 0.5178 | 0.4959 |
Cytoplasmic Foci | 0.0228 | 0.0077 | 0.0154 | 0.0085 | 0.0198 | 0.0095 | 0.0212 | 0.0203 | 0.0327 | 0.0133 | 0.0603 | 0.0271 | 0.0174 | 0.0052 | 0.0073 | 0.0148 | 0.0155 | 0.0051 |
Eisosomes | 0.0044 | 0.0067 | 0.005 | 0.002 | 0.002 | 0.0018 | 0.0104 | 0.0064 | 0.0068 | 0.0048 | 0.0014 | 0.0025 | 0.0126 | 0.0082 | 0.0091 | 0.0032 | 0.0028 | 0.0029 |
Endoplasmic Reticulum | 0.0084 | 0.0048 | 0.0093 | 0.004 | 0.0059 | 0.0049 | 0.017 | 0.005 | 0.0083 | 0.0189 | 0.0075 | 0.0085 | 0.0057 | 0.0018 | 0.0043 | 0.0082 | 0.0044 | 0.0034 |
Endosome | 0.0199 | 0.0008 | 0.0044 | 0.0013 | 0.0296 | 0.0057 | 0.0384 | 0.0013 | 0.004 | 0.0105 | 0.0308 | 0.0039 | 0.0061 | 0.0003 | 0.0013 | 0.0027 | 0.0017 | 0.0008 |
Golgi | 0.0087 | 0.002 | 0.0042 | 0.002 | 0.0196 | 0.003 | 0.0137 | 0.0029 | 0.0035 | 0.0039 | 0.009 | 0.0035 | 0.005 | 0.0014 | 0.0025 | 0.0039 | 0.0016 | 0.0017 |
Lipid Particles | 0.0177 | 0.0019 | 0.0031 | 0.0027 | 0.0169 | 0.0057 | 0.0099 | 0.0039 | 0.0025 | 0.003 | 0.017 | 0.0022 | 0.0036 | 0.0008 | 0.0027 | 0.0172 | 0.0031 | 0.0018 |
Mitochondria | 0.0091 | 0.0084 | 0.0106 | 0.0494 | 0.0137 | 0.0038 | 0.0059 | 0.001 | 0.0035 | 0.0033 | 0.0039 | 0.0016 | 0.0011 | 0.0001 | 0.0019 | 0.0181 | 0.0004 | 0.0009 |
None | 0.2541 | 0.2658 | 0.1823 | 0.3245 | 0.2732 | 0.2561 | 0.2983 | 0.329 | 0.2622 | 0.2025 | 0.2106 | 0.3784 | 0.2719 | 0.1404 | 0.2673 | 0.2726 | 0.2777 | 0.3424 |
Nuclear Periphery | 0.0122 | 0.0003 | 0.0029 | 0.0004 | 0.0016 | 0.0077 | 0.0284 | 0.0004 | 0.0048 | 0.0226 | 0.0258 | 0.0034 | 0.0032 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0003 |
Nucleolus | 0.0023 | 0.0003 | 0.0008 | 0.0011 | 0.0143 | 0.0008 | 0.0036 | 0.0012 | 0.0029 | 0.004 | 0.0027 | 0.0027 | 0.0004 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0002 |
Nucleus | 0.0214 | 0.0005 | 0.0024 | 0.0011 | 0.0051 | 0.0019 | 0.0123 | 0.0018 | 0.0125 | 0.0134 | 0.0095 | 0.0057 | 0.0054 | 0.0004 | 0.003 | 0.0004 | 0.0005 | 0.0004 |
Peroxisomes | 0.0159 | 0.0004 | 0.0034 | 0.0024 | 0.0094 | 0.0045 | 0.0018 | 0.001 | 0.003 | 0.0009 | 0.0025 | 0.001 | 0.0023 | 0.0003 | 0.0014 | 0.017 | 0.0007 | 0.0016 |
Punctate Nuclear | 0.0074 | 0.0007 | 0.0028 | 0.0009 | 0.003 | 0.0031 | 0.0101 | 0.0022 | 0.0053 | 0.0074 | 0.0171 | 0.0057 | 0.0007 | 0.0002 | 0.001 | 0.0003 | 0.0012 | 0.0004 |
Vacuole | 0.0034 | 0.001 | 0.0029 | 0.0027 | 0.0088 | 0.0016 | 0.01 | 0.0018 | 0.0022 | 0.0069 | 0.0032 | 0.0049 | 0.0057 | 0.0009 | 0.0015 | 0.0075 | 0.0016 | 0.0014 |
Vacuole Periphery | 0.001 | 0 | 0.0005 | 0.0002 | 0.0014 | 0.0006 | 0.0031 | 0.0001 | 0.0006 | 0.0035 | 0.0017 | 0.0004 | 0.001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.4142 | 11.0274 | 9.4749 | 11.2868 | 11.1536 | 16.2223 | 13.2771 | 16.338 | 13.2835 | 12.9613 |
Translational Efficiency | 1.4275 | 1.1291 | 1.0975 | 0.9667 | 1.0022 | 0.9207 | 0.8231 | 0.7099 | 0.971 | 0.9459 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |