Standard name
Human Ortholog
Description Eisosome component; likely plays only a minor role in eisosome assembly; shown to interact with Seg1p by affinity purification and mass spec; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; similar to <i>A. gossypii</i> SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.32 0.21 0.11 0 0.06 0 0 0 0 – 0 0 0.17 0.14 0.09 0.33 0.34 0.26 0.45 0.34 0.33 0.25 0.29 0.36
Bud 0 0 0.06 0 0 0 0 0 0 – 0 0 0.05 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0
Bud Neck 0 0.05 0.09 0.06 0.06 0.07 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0.06 0.1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.13 0.14 0.06 0.06 0.06 0.08 0 0 0 – 0.07 0.05 0.22 0.2 0.26 0.32 0.28 0.33 0.34 0.37 0.37 0.48 0.42 0.37
Cytoplasm 0.53 0.67 0.81 0.86 0.7 0.77 0.79 0.89 0.89 – 0.9 0.9 0.74 0.84 0.87 0.33 0.36 0.38 0.05 0.08 0 0.07 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.06 0 0 0.05 0.09 0.05 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.11 0 0 0.18 0.33 0.23 0.2 0.13 0.18 – 0.17 0.18 0 0 0 0.11 0.17 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 61 62 48 3 15 8 0 7 0 – 0 0 20 36 27 63 58 37 115 121 171 35 58 73
Bud 6 10 26 0 0 0 0 0 0 – 0 0 6 10 15 9 8 9 0 0 2 1 1 1
Bud Neck 1 15 42 13 16 19 3 3 0 – 0 2 2 6 6 0 7 0 5 24 25 10 11 21
Bud Site 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 1 – – – – – –
Cell Periphery 25 42 26 13 15 21 10 11 7 – 14 11 27 51 75 61 49 47 85 134 192 68 85 77
Cytoplasm 101 197 362 178 183 203 217 253 238 – 179 202 90 218 251 63 62 53 11 27 23 10 13 9
Endoplasmic Reticulum 1 8 5 0 0 0 0 0 0 – 0 0 1 1 1 13 13 12 6 7 14 1 3 0
Endosome 1 1 0 0 0 0 0 0 1 – 0 1 0 0 0 1 0 2 5 5 8 0 1 4
Golgi 1 0 0 0 0 0 0 0 0 – 1 0 0 0 0 1 1 0 3 3 1 0 1 1
Mitochondria 5 10 2 0 0 0 0 0 0 – 0 0 0 0 3 13 10 4 6 16 44 7 16 10
Nucleus 2 1 0 0 1 0 3 1 1 – 0 0 0 0 0 1 5 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 9 0 0 0 0 0 0 0 0 – 0 0 0 2 0 0 1 3 5 3 8 0 2 2
SpindlePole 21 5 17 38 85 60 55 37 49 – 34 41 1 9 5 21 29 11 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 6 5 19 3 4 – 1 1 1 0 0 2 4 1 1 7 4 2 1 0
Unique Cell Count 190 293 447 207 261 262 273 285 267 199 224 121 260 290 190 172 141 253 363 515 143 202 207
Labelled Cell Count 235 352 529 245 321 316 307 316 300 229 258 148 333 383 251 247 180 253 363 515 143 202 207


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.3 3.8 7.2 7.8 7.2 7.1 7.1 7.7 – 8.6 8.6 4.7 5.0 4.5 7.0 7.5 7.4 4.8 5.2 5.4
Std Deviation (1e-4) 1.1 0.7 0.6 0.7 5.8 0.8 0.8 0.9 0.8 – 0.6 0.7 0.9 1.0 0.9 1.4 1.7 1.9 1.3 1.5 1.5
Intensity Change (Log2) – – – 0.94 1.06 0.95 0.93 0.93 1.04 – 1.19 1.2 0.31 0.42 0.26 0.91 1.0 0.97 0.34 0.46 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2948 1.897 1.8104 1.3309 1.8657 1.8217 -0.1674 -0.7512 -0.698 -1.0385 -0.2431 -0.7211 4.3916 3.9567 3.9174 4.2882 3.7445 4.2216
Actin 0.0834 0.0288 0.0609 0.0349 0.0222 0.0248 0.0571 0.0483 0.0588 0.1159 0.1139 0.0326 0.0681 0.1209 0.0466 0.1084 0.0474 0.0216
Bud 0.0077 0.0011 0.005 0.001 0.0022 0.0033 0.0096 0.0084 0.0061 0.0233 0.0009 0.0065 0.0012 0.0004 0.0064 0.0008 0.0016 0.0021
Bud Neck 0.0234 0.0056 0.0419 0.0477 0.0249 0.0422 0.0103 0.011 0.0244 0.018 0.0254 0.0298 0.0125 0.0118 0.0485 0.0188 0.0373 0.0227
Bud Periphery 0.0214 0.0035 0.0187 0.0052 0.0085 0.0207 0.0165 0.0091 0.012 0.0435 0.0037 0.0166 0.0054 0.0016 0.0176 0.0058 0.0083 0.0154
Bud Site 0.0482 0.1407 0.0977 0.0117 0.0075 0.0066 0.0548 0.1734 0.0861 0.0211 0.0042 0.0051 0.0503 0.1295 0.084 0.0042 0.009 0.0065
Cell Periphery 0.0706 0.0623 0.0849 0.0381 0.0532 0.0536 0.0875 0.0386 0.0578 0.0406 0.0292 0.041 0.1036 0.0826 0.0804 0.0558 0.0666 0.0725
Cytoplasm 0.3365 0.4567 0.4409 0.4583 0.4574 0.5382 0.2803 0.333 0.3998 0.4188 0.4199 0.4171 0.417 0.4928 0.4117 0.4394 0.5178 0.4959
Cytoplasmic Foci 0.0228 0.0077 0.0154 0.0085 0.0198 0.0095 0.0212 0.0203 0.0327 0.0133 0.0603 0.0271 0.0174 0.0052 0.0073 0.0148 0.0155 0.0051
Eisosomes 0.0044 0.0067 0.005 0.002 0.002 0.0018 0.0104 0.0064 0.0068 0.0048 0.0014 0.0025 0.0126 0.0082 0.0091 0.0032 0.0028 0.0029
Endoplasmic Reticulum 0.0084 0.0048 0.0093 0.004 0.0059 0.0049 0.017 0.005 0.0083 0.0189 0.0075 0.0085 0.0057 0.0018 0.0043 0.0082 0.0044 0.0034
Endosome 0.0199 0.0008 0.0044 0.0013 0.0296 0.0057 0.0384 0.0013 0.004 0.0105 0.0308 0.0039 0.0061 0.0003 0.0013 0.0027 0.0017 0.0008
Golgi 0.0087 0.002 0.0042 0.002 0.0196 0.003 0.0137 0.0029 0.0035 0.0039 0.009 0.0035 0.005 0.0014 0.0025 0.0039 0.0016 0.0017
Lipid Particles 0.0177 0.0019 0.0031 0.0027 0.0169 0.0057 0.0099 0.0039 0.0025 0.003 0.017 0.0022 0.0036 0.0008 0.0027 0.0172 0.0031 0.0018
Mitochondria 0.0091 0.0084 0.0106 0.0494 0.0137 0.0038 0.0059 0.001 0.0035 0.0033 0.0039 0.0016 0.0011 0.0001 0.0019 0.0181 0.0004 0.0009
None 0.2541 0.2658 0.1823 0.3245 0.2732 0.2561 0.2983 0.329 0.2622 0.2025 0.2106 0.3784 0.2719 0.1404 0.2673 0.2726 0.2777 0.3424
Nuclear Periphery 0.0122 0.0003 0.0029 0.0004 0.0016 0.0077 0.0284 0.0004 0.0048 0.0226 0.0258 0.0034 0.0032 0.0001 0.0006 0.0003 0.0004 0.0003
Nucleolus 0.0023 0.0003 0.0008 0.0011 0.0143 0.0008 0.0036 0.0012 0.0029 0.004 0.0027 0.0027 0.0004 0.0002 0.0007 0.0003 0.0002 0.0002
Nucleus 0.0214 0.0005 0.0024 0.0011 0.0051 0.0019 0.0123 0.0018 0.0125 0.0134 0.0095 0.0057 0.0054 0.0004 0.003 0.0004 0.0005 0.0004
Peroxisomes 0.0159 0.0004 0.0034 0.0024 0.0094 0.0045 0.0018 0.001 0.003 0.0009 0.0025 0.001 0.0023 0.0003 0.0014 0.017 0.0007 0.0016
Punctate Nuclear 0.0074 0.0007 0.0028 0.0009 0.003 0.0031 0.0101 0.0022 0.0053 0.0074 0.0171 0.0057 0.0007 0.0002 0.001 0.0003 0.0012 0.0004
Vacuole 0.0034 0.001 0.0029 0.0027 0.0088 0.0016 0.01 0.0018 0.0022 0.0069 0.0032 0.0049 0.0057 0.0009 0.0015 0.0075 0.0016 0.0014
Vacuole Periphery 0.001 0 0.0005 0.0002 0.0014 0.0006 0.0031 0.0001 0.0006 0.0035 0.0017 0.0004 0.001 0 0.0001 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4142 11.0274 9.4749 11.2868 11.1536 16.2223 13.2771 16.338 13.2835 12.9613
Translational Efficiency 1.4275 1.1291 1.0975 0.9667 1.0022 0.9207 0.8231 0.7099 0.971 0.9459

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
244 1362 147 1402 1077 1914 1358 736 1321 3276 1505 2138

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.17 670.94 1073.43 838.08 671.83 706.39 788.85 795.24 663.95 691.65 816.65 823.33
Standard Deviation 62.99 92.22 111.03 113.15 103.57 84.38 107.74 101.06 98.76 89.45 137.17 111.02
Intensity Change Log 2 — 0.092734 0.770706 0.413638 — 0.072369 0.231655 0.243294 — 0.082253 0.517449 0.328187

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.050678 0.083207 0.141701* 0.118808* 0.042429 0.058907 0.070469 0.051150 0.043953 0.069010 0.077427 0.095517
Bud Neck 0.067043 0.041784 0.023134 0.026394 0.030888 0.067702 0.025575 0.025082 0.037566 0.056927 0.025337 0.025942
Bud Site 0.007373 0.021049 0.169669* 0.098516 0.009273 0.036212 0.038626 0.042047 0.008922 0.029908 0.051426 0.079076
Cell Periphery 0.035367 0.008715 0.077379 0.049280 0.015479 0.012023 0.017842 0.022585 0.019152 0.010648 0.023657 0.040090
Cytoplasm 0.352724* 0.261380* 0.140693* 0.280767* 0.388967* 0.391308* 0.388209* 0.353486* 0.382272* 0.337290* 0.364033* 0.305800*
Cytoplasmic Foci 0.098756 0.172956 0.080995 0.042337 0.121927 0.131921 0.105438 0.113659 0.117647 0.148981 0.103051 0.066889
Eisosomes 0.174571* 0.057860 0.035773 0.072666 0.104887* 0.082546 0.029116 0.031742 0.117758* 0.072283 0.029766 0.058578
Endoplasmic Reticulum 0.001691 0.004654 0.079423 0.012906 0.003470 0.002049 0.014757 0.008282 0.003141 0.003132 0.021073 0.011314
Endosome 0.008279 0.021356 0.010084 0.004068 0.006317 0.011423 0.007154 0.009138 0.006680 0.015553 0.007440 0.005813
Golgi 0.020420 0.070812 0.006835 0.002073 0.047431 0.029896 0.013379 0.004552 0.042442 0.046907 0.012739 0.002926
Lipid Particles 0.042824 0.071889 0.018239 0.007745 0.066644 0.026024 0.041279 0.029554 0.062244 0.045093 0.039029 0.015253
Mitochondria 0.008034 0.018721 0.000176 0.002017 0.008102 0.016372 0.001080 0.001630 0.008090 0.017349 0.000991 0.001884
Mitotic Spindle 0.000276 0.000619 0.001256 0.006290 0.000229 0.002118 0.000626 0.003124 0.000238 0.001495 0.000687 0.005200
None 0.000512 0.001046 0.000104 0.002278 0.002159 0.001244 0.001391 0.001210 0.001855 0.001162 0.001265 0.001910
Nuclear Periphery 0.000117 0.000199 0.000209 0.000251 0.000135 0.000068 0.000265 0.000353 0.000132 0.000122 0.000260 0.000287
Nuclear Periphery Foci 0.000128 0.000367 0.010498 0.001428 0.000284 0.000234 0.002300 0.002054 0.000255 0.000289 0.003101 0.001644
Nucleolus 0.000108 0.000302 0.000082 0.000096 0.000318 0.000218 0.000189 0.000186 0.000279 0.000253 0.000179 0.000127
Nucleus 0.031435 0.028944 0.038907 0.132948* 0.063582 0.048050 0.163304* 0.125006 0.057645 0.040107 0.151154* 0.130214*
Peroxisomes 0.084272 0.113254* 0.003482 0.008736 0.077207 0.059237 0.022806 0.028290 0.078512 0.081695 0.020919 0.015467
Vacuole 0.015046 0.019466 0.161190 0.128995 0.009897 0.021613 0.056013 0.145362 0.010848 0.020720 0.066286 0.134629
Vacuole Periphery 0.000346 0.001421 0.000171 0.001403 0.000375 0.000835 0.000183 0.001508 0.000370 0.001079 0.000182 0.001439

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.45 -5.22 -6.14 -4.11 1.70 -3.78 -4.93 -0.94 1.98 3.30 -6.37 -6.12 -9.31 -4.43 -2.39
Bud Neck 2.79 4.78 4.75 6.16 -0.77 -10.62 2.00 2.13 12.43 0.53 -6.21 4.30 4.63 15.01 0.17
Bud Site -7.20 -11.08 -18.24 -15.23 4.64 -11.70 -9.94 -7.39 -1.03 -0.58 -13.39 -13.38 -19.34 -12.65 -5.76
Cell Periphery 4.94 -4.11 -1.77 -15.10 3.51 2.04 -0.94 -1.95 -3.58 -1.25 5.03 -1.71 -7.69 -14.58 -6.00
Cytoplasm 5.24 10.21 4.37 -1.79 -9.52 -0.25 0.25 3.64 4.39 3.63 5.36 2.06 9.28 5.53 7.35
Cytoplasmic Foci -6.96 1.35 5.59 25.04 4.39 -1.82 2.70 1.56 3.32 -0.83 -6.45 2.58 10.96 22.16 8.35
Eisosomes 7.69 8.97 7.07 -1.94 -5.47 3.75 14.34 13.04 11.93 -0.21 8.33 16.85 11.54 5.43 -8.05
Endoplasmic Reticulum -3.96 -9.15 -10.90 -8.23 7.77 2.86 -7.16 -4.65 -6.35 3.56 0.01 -10.48 -10.91 -11.56 5.30
Endosome -6.65 -1.10 2.80 11.14 4.63 -5.53 -0.96 -1.71 1.89 -1.13 -9.96 -0.97 1.43 10.32 2.24
Golgi -13.27 5.63 8.12 22.52 4.71 6.32 12.62 16.71 17.47 6.45 -1.73 12.92 18.89 28.70 8.94
Lipid Particles -3.97 3.69 5.44 17.67 5.49 9.30 5.11 7.53 -0.65 3.03 4.31 5.31 12.65 14.48 8.31
Mitochondria -2.84 2.27 1.76 10.58 -2.75 -5.75 8.13 5.64 10.62 -0.57 -7.04 7.46 5.97 15.14 -1.54
Mitotic Spindle -0.76 -0.98 -3.90 -3.52 -2.67 -3.07 -1.43 -1.71 -0.47 -1.45 -3.10 -1.68 -4.25 -2.91 -3.76
None -3.04 3.98 -1.72 -1.13 -2.18 1.75 1.68 1.57 0.08 0.40 1.81 1.53 0.10 -0.95 -0.77
Nuclear Periphery -1.61 -3.47 -3.05 -1.07 -0.89 1.57 -3.14 -2.19 -3.10 -0.89 0.24 -3.73 -3.01 -3.72 -0.59
Nuclear Periphery Foci -3.63 -9.92 -11.86 -8.76 8.51 0.71 -7.35 -4.63 -4.86 0.64 -0.31 -10.40 -9.01 -9.28 4.59
Nucleolus -3.14 1.14 0.57 3.44 -0.70 0.86 1.09 1.10 1.05 0.11 0.30 1.04 1.61 4.40 3.24
Nucleus 0.71 -1.53 -19.57 -23.36 -13.94 4.43 -17.11 -10.08 -13.73 5.25 6.17 -17.74 -17.55 -25.66 3.65
Peroxisomes -2.82 8.51 7.97 26.22* -5.19 4.11 13.72 10.21 8.17 -1.03 -0.78 15.49 17.17 26.79 3.41
Vacuole -1.32 -10.41 -22.35 -24.26 1.44 -9.29 -17.96 -21.05 -19.34 -13.68 -8.55 -19.52 -33.54 -31.08 -16.60
Vacuole Periphery -4.03 1.96 -1.65 0.24 -1.98 -2.32 3.09 -1.50 -0.78 -1.80 -4.41 3.48 -2.16 -0.50 -2.61