Standard name
Human Ortholog
Description Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0.07 0.07 0.11 0.05 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0.06 0 0
Bud 0 0 0 0 0 0 0 0.06 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.06 0 0.12 0.05 0.05 0.05 0.07 0.05 0.06 0.05 0.08 0.09 0.09 0.12 0.24 0.29 0.22 0 0 0 0 0 0
Cytoplasm 0.88 0.88 0.87 0.85 0.74 0.78 0.82 0.74 0.77 0.76 0.78 0.93 0.98 0.98 0.77 0.82 0.83 0.8 0.91 0.65 0.79 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.08 0.18 0.15 0.24 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 0 17 26 29 21 17 15 15 2 12 12 2 4 8 4 7 11 0 1 9 1 3
Bud 8 0 7 5 10 16 15 20 24 11 19 8 1 0 2 0 3 4 0 0 0 1 1
Bud Neck 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 4 1 4 5 5 3
Bud Site 1 0 2 0 2 4 0 1 1 0 0 0 0 0 1 0 0
Cell Periphery 17 1 29 17 14 19 33 17 25 12 24 28 29 49 40 38 22 0 0 0 2 1 5
Cytoplasm 244 21 210 295 200 320 391 267 308 173 239 284 319 393 130 107 85 218 32 56 137 345 421
Endoplasmic Reticulum 8 0 5 11 19 12 19 9 8 4 3 12 6 2 5 5 4 10 0 1 5 9 19
Endosome 6 1 0 6 10 18 2 4 2 2 1 2 1 1 4 0 2 7 0 6 3 1 3
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 6 1 0 0 0 0 0 2
Mitochondria 3 0 0 0 11 31 39 64 60 55 46 0 0 0 3 1 1 1 0 2 1 3 2
Nucleus 0 1 1 0 1 1 2 2 2 1 2 1 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 1 1 2 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3 0 0 0 2 2 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 12 0 12 15 19 14 11 6 13 2 6 2 3 4 12 6 3 9 0 4 2 0 0
Vac/Vac Membrane 0 0 2 4 5 17 7 12 13 5 11 0 0 0 7 8 2 0 0 5 1 1 2
Unique Cell Count 278 24 242 347 270 410 476 361 399 229 307 307 327 401 169 131 102 273 36 86 174 375 472
Labelled Cell Count 319 25 286 380 323 476 537 424 473 268 364 349 362 453 214 177 130 273 36 86 174 375 472


Cytoplasm, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 7.1 8.3 8.2 7.5 7.0 7.4 6.4 6.5 6.1 6.3 8.7 9.1 8.9 13.1 13.5 13.5 8.2 8.4 8.9
Std Deviation (1e-4) 1.0 0.8 1.2 1.2 1.5 1.5 1.2 1.0 1.0 1.4 1.1 1.0 1.1 1.1 2.1 2.7 2.7 1.2 1.2 1.2
Intensity Change (Log2) 0.22 0.2 0.08 -0.01 0.06 -0.16 -0.12 -0.23 -0.16 0.3 0.35 0.33 0.88 0.93 0.92 0.21 0.23 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.2 2.6 0
Cytoplasm -0.1 -0.3 -1.5 -1.1 -0.7 -1.5 -1.2 -1.3 -1.1 0.9 2.7 3.1 -1.2 -0.7 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9251 7.3274 6.3561 6.0227 6.4052 7.5092 5.7232 6.5104 5.7769 5.7755 5.1498 6.6667 5.8245 5.6343 4.9487 4.1111 6.0296 5.9643
Actin 0.0388 0.002 0.0225 0.0377 0.0064 0.0269 0.0345 0.002 0.007 0.0397 0.0415 0.0094 0.0272 0.0006 0.0104 0.0025 0.0005 0.0319
Bud 0.0012 0.0011 0.001 0.0087 0.0003 0.0003 0.0006 0.0005 0.0007 0.0003 0.0013 0.0002 0.0003 0.0003 0.0003 0.0004 0.0001 0.0005
Bud Neck 0.0035 0.0007 0.0039 0.0015 0.001 0.1095 0.0029 0.0012 0.0014 0.0027 0.0016 0.2112 0.0031 0.0003 0.0006 0.0343 0.0008 0.1767
Bud Periphery 0.0015 0.0005 0.001 0.017 0.0002 0.0004 0.0008 0.0003 0.0007 0.0002 0.0015 0.0003 0.0005 0.0001 0.0002 0.0004 0 0.0006
Bud Site 0.0121 0.0208 0.0084 0.0195 0.001 0.0009 0.0068 0.0083 0.0158 0.0015 0.0082 0.0006 0.0014 0.0035 0.0021 0.0019 0.0006 0.0018
Cell Periphery 0.0009 0.0006 0.0008 0.0015 0 0.0003 0.0005 0.0009 0.0006 0.0003 0.0004 0.0002 0.0004 0.0003 0.0003 0.0002 0.0001 0.0003
Cytoplasm 0.7863 0.9003 0.8474 0.6475 0.2598 0.575 0.7148 0.9027 0.9022 0.73 0.2116 0.4379 0.8091 0.9122 0.8831 0.7629 0.2714 0.5234
Cytoplasmic Foci 0.0441 0.051 0.02 0.0925 0.4815 0.1395 0.0671 0.0504 0.0208 0.1072 0.4232 0.1757 0.039 0.0487 0.0348 0.0226 0.4704 0.1142
Eisosomes 0.0003 0 0.0003 0.0004 0 0 0.0004 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0001 0.0003 0 0.0001
Endoplasmic Reticulum 0.022 0.0042 0.0104 0.0249 0.0016 0.0079 0.0343 0.0053 0.0149 0.0186 0.0133 0.0054 0.0485 0.0121 0.018 0.005 0.005 0.029
Endosome 0.0299 0.0043 0.0251 0.0547 0.0854 0.0169 0.0447 0.0085 0.0121 0.0349 0.136 0.0132 0.0227 0.007 0.0247 0.0367 0.195 0.0182
Golgi 0.013 0.002 0.018 0.0318 0.0163 0.0126 0.0121 0.0055 0.0027 0.0177 0.0149 0.0065 0.007 0.0008 0.007 0.0106 0.0142 0.0085
Lipid Particles 0.009 0.0019 0.0045 0.0044 0.011 0.0104 0.021 0.0032 0.0043 0.0203 0.03 0.0138 0.0084 0.0004 0.0046 0.0074 0.014 0.0178
Mitochondria 0.0116 0.0001 0.0194 0.031 0.0586 0.002 0.0135 0.001 0.0017 0.0017 0.0052 0.0104 0.0023 0.0001 0.0013 0.0099 0.0003 0.0182
None 0.0048 0.0027 0.006 0.0018 0.0007 0.0009 0.0114 0.0014 0.0057 0.0017 0.0012 0.0009 0.0047 0.0069 0.0019 0.094 0.0009 0.0009
Nuclear Periphery 0.0031 0.0004 0.002 0.0018 0.0005 0.0075 0.0041 0.0004 0.0009 0.0014 0.0033 0.0064 0.0051 0.0005 0.001 0.0009 0.001 0.0014
Nucleolus 0.0006 0 0.0001 0.0006 0.0001 0.0002 0.0004 0.0001 0.0004 0.0001 0.001 0.0003 0.0001 0 0.0001 0.0005 0.0001 0.0003
Nucleus 0.0017 0.0006 0.001 0.0015 0.0005 0.0014 0.0016 0.0006 0.001 0.0008 0.0023 0.0014 0.0012 0.0006 0.0007 0.0009 0.0006 0.0019
Peroxisomes 0.0062 0.0033 0.0027 0.0078 0.0554 0.014 0.0117 0.0032 0.0014 0.0145 0.0376 0.0133 0.0134 0.0002 0.0027 0.0036 0.0053 0.0068
Punctate Nuclear 0.0022 0.0023 0.0008 0.0035 0.0167 0.0719 0.0125 0.003 0.0024 0.0031 0.0466 0.0915 0.0018 0.0035 0.0019 0.0006 0.007 0.0456
Vacuole 0.0059 0.0009 0.0034 0.0074 0.0026 0.0009 0.003 0.0015 0.0027 0.0028 0.0148 0.0009 0.0029 0.0018 0.0036 0.0038 0.0119 0.0016
Vacuole Periphery 0.0014 0.0001 0.0013 0.0023 0.0006 0.0006 0.0013 0.0001 0.0006 0.0004 0.0045 0.0004 0.0007 0.0002 0.0005 0.0007 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7572 36.5325 38.7266 36.5579 20.4373 12.8368 36.4102 50.5735 38.2971 20.237
Translational Efficiency 1.9348 1.9584 1.8693 1.5067 1.1133 1.4093 2.0479 1.5424 1.5684 1.3546

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1419 330 2405 1448 1227 1550 1026 917 2646 1880 3431 2365

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 915.72 1082.61 1114.41 1211.80 939.94 1031.19 1228.95 1271.65 926.95 1040.22 1148.66 1235.01
Standard Deviation 112.37 146.39 142.36 217.42 123.36 134.08 164.29 196.84 118.21 137.71 158.20 211.70
Intensity Change Log 2 0.241535 0.283302 0.404173 0.133670 0.386786 0.436061 0.187906 0.336646 0.420413

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003535 0.006167 0.010736 0.028246 0.003161 0.003527 0.012005 0.015744 0.003361 0.003990 0.011115 0.023398
Bud Neck 0.025421 0.011574 0.014120 0.012079 0.020802 0.017078 0.009672 0.012351 0.023279 0.016112 0.012790 0.012184
Bud Site 0.061292 0.092670 0.094520 0.161671* 0.069875 0.080325 0.108938* 0.123898* 0.065272 0.082492 0.098831 0.147025*
Cell Periphery 0.002129 0.002076 0.000283 0.001453 0.001824 0.000707 0.000573 0.000953 0.001987 0.000947 0.000370 0.001259
Cytoplasm 0.470406* 0.481713* 0.494863* 0.449910* 0.522979* 0.489616* 0.471497* 0.340115* 0.494785* 0.488228* 0.487876* 0.407338*
Cytoplasmic Foci 0.278989* 0.287306* 0.155592 0.173841 0.257787* 0.265045* 0.218126* 0.210463* 0.269157* 0.268952* 0.174292 0.188041*
Eisosomes 0.000945 0.000187 0.000052 0.000123 0.000650 0.000206 0.000065 0.000107 0.000808 0.000203 0.000056 0.000117
Endoplasmic Reticulum 0.011837 0.004860 0.000415 0.001108 0.009279 0.004785 0.001140 0.001009 0.010651 0.004798 0.000632 0.001070
Endosome 0.024567 0.020709 0.015020 0.012198 0.016986 0.020875 0.009905 0.024890 0.021051 0.020846 0.013490 0.017119
Golgi 0.019474 0.010509 0.002361 0.005527 0.014245 0.014278 0.001900 0.007099 0.017049 0.013617 0.002223 0.006137
Lipid Particles 0.011974 0.011134 0.006473 0.041969 0.008989 0.006122 0.016589 0.030623 0.010589 0.007002 0.009498 0.037570
Mitochondria 0.001398 0.001073 0.000681 0.000471 0.001027 0.001210 0.000181 0.004988 0.001226 0.001186 0.000531 0.002222
Mitotic Spindle 0.002284 0.009706 0.050142 0.018790 0.001722 0.011198 0.039810 0.057426 0.002024 0.010936 0.047053 0.033771
None 0.000658 0.000220 0.000654 0.000295 0.001046 0.001059 0.000753 0.000208 0.000838 0.000911 0.000684 0.000261
Nuclear Periphery 0.000051 0.000116 0.000083 0.000057 0.000056 0.000037 0.000080 0.000171 0.000053 0.000051 0.000082 0.000101
Nuclear Periphery Foci 0.001586 0.002951 0.001451 0.005517 0.002302 0.001516 0.012286 0.004579 0.001918 0.001768 0.004691 0.005153
Nucleolus 0.000161 0.000067 0.000284 0.000076 0.000290 0.000142 0.000070 0.000275 0.000221 0.000129 0.000220 0.000153
Nucleus 0.007081 0.002407 0.005655 0.003851 0.008609 0.004981 0.006733 0.003054 0.007790 0.004529 0.005977 0.003542
Peroxisomes 0.007699 0.006072 0.030900 0.020683 0.007491 0.009809 0.019972 0.020056 0.007602 0.009153 0.027632 0.020440
Vacuole 0.066081 0.043306 0.114760 0.061151 0.049174 0.065351 0.069536 0.139095 0.058241 0.061481 0.101236 0.091373
Vacuole Periphery 0.002433 0.005179 0.000957 0.000986 0.001708 0.002133 0.000168 0.002894 0.002097 0.002668 0.000721 0.001726

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.20 -4.73 -9.97 -6.31 -6.93 -0.59 -5.16 -5.76 -5.59 -0.92 -0.97 -6.81 -11.49 -10.86 -6.56
Bud Neck 3.83 6.01 6.95 0.36 1.70 1.50 5.87 5.21 4.16 -1.08 3.63 7.80 8.59 3.87 1.31
Bud Site -3.87 -5.37 -13.39 -5.34 -9.49 -1.88 -4.02 -4.77 -3.23 -0.79 -4.11 -6.28 -13.51 -8.61 -8.32
Cell Periphery 0.15 8.69 3.04 2.01 -6.29 8.90 10.97 6.89 -0.29 -1.84 7.11 12.92 5.39 -1.08 -6.72
Cytoplasm -0.59 -1.53 1.87 1.68 3.24 3.14 3.50 13.57 11.04 9.12 0.94 1.45 9.87 7.88 8.30
Cytoplasmic Foci -0.52 14.37 11.75 7.11 -1.50 -1.02 4.52 5.38 6.62 0.96 -0.21 14.58 12.42 11.34 -1.17
Eisosomes 9.55 12.46 11.45 2.14 -5.70 8.59 11.82 11.20 7.45 -3.30 12.92 16.77 15.48 6.72 -6.62
Endoplasmic Reticulum 3.38 7.42 7.04 2.84 -3.42 3.18 6.74 6.96 5.40 1.43 4.93 10.05 9.72 5.93 -2.46
Endosome 1.26 7.52 9.67 3.38 2.87 -2.66 6.43 -1.09 0.96 -5.65 0.19 8.79 6.50 5.31 -1.36
Golgi 7.23 19.02 13.61 5.78 -3.13 0.05 18.90 8.26 6.84 -2.63 3.37 25.83 15.90 8.98 -4.00
Lipid Particles 0.25 4.22 -10.46 -8.19 -12.46 3.79 -4.20 -6.66 -7.79 -3.48 4.18 0.99 -12.54 -14.18 -12.79
Mitochondria 0.38 2.77 3.40 0.85 1.13 -0.35 2.25 -1.45 -1.34 -2.30 0.14 3.15 -0.13 -0.21 -1.84
Mitotic Spindle -2.03 -15.92 -7.91 -3.10 8.57 -5.24 -8.72 -10.45 -8.39 -2.96 -5.48 -18.23 -13.01 -8.50 4.22
None 2.55 0.22 2.10 -1.15 0.87 0.03 0.89 2.10 2.28 1.24 -0.15 0.81 2.77 2.11 1.31
Nuclear Periphery -0.76 -7.74 -4.40 0.39 4.22 1.03 -3.06 -4.86 -6.60 -3.42 0.13 -6.39 -5.73 -4.23 -0.86
Nuclear Periphery Foci -2.35 -1.31 -8.58 -4.51 -7.71 1.77 -11.18 -5.58 -7.71 8.47 0.24 -9.00 -10.00 -9.75 -0.72
Nucleolus 5.79 -1.83 2.52 -0.85 2.41 1.06 1.51 0.48 -0.62 -1.23 1.40 -0.54 1.07 -0.18 1.59
Nucleus 12.94 1.37 3.88 -7.37 2.17 4.66 0.36 5.32 1.87 7.11 7.66 1.67 6.35 -1.16 5.44
Peroxisomes 0.97 -9.19 -5.53 -5.38 3.98 -1.81 -3.51 -4.45 -3.01 -0.06 -1.50 -9.97 -7.13 -5.31 3.71
Vacuole 4.73 -10.94 -4.31 -8.10 6.80 -4.43 -9.66 -17.38 -14.74 -11.54 -1.02 -14.91 -16.00 -13.82 -2.85
Vacuole Periphery -1.08 6.02 5.59 1.76 0.18 -0.89 5.56 0.05 0.62 -2.53 -0.99 6.89 3.11 2.59 -1.58