Standard name | |
---|---|
Human Ortholog | |
Description | Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 2 | 0 | 3 | 0 | 15 | 17 | 14 | 14 | 18 | 23 | 3 | 3 | 4 | 10 | 0 | 2 | 1 | 1 | 11 | 2 | 5 | 1 |
Bud | 0 | 0 | 0 | 2 | 0 | 5 | 6 | 8 | 17 | 13 | 19 | 25 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 7 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 10 | 3 | 5 | 8 | 6 | 6 | 6 | 8 | 9 | 16 | 5 | 5 | 24 | 15 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 5 | 9 | 18 | 24 | 17 | 24 | 40 | 22 | 34 | 35 | 26 | 7 | 8 | 22 | 15 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 91 | 58 | 107 | 82 | 107 | 85 | 151 | 139 | 95 | 84 | 124 | 99 | 46 | 59 | 118 | 3 | 0 | 0 | 173 | 88 | 317 | 89 | 136 | 148 |
Endosome | 0 | 1 | 4 | 10 | 10 | 18 | 26 | 43 | 22 | 44 | 28 | 48 | 1 | 1 | 3 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 2 | 1 |
Golgi | 8 | 0 | 21 | 7 | 9 | 1 | 6 | 5 | 1 | 0 | 3 | 1 | 2 | 1 | 1 | 17 | 8 | 10 | 0 | 0 | 4 | 2 | 1 | 0 |
Mitochondria | 4 | 1 | 16 | 21 | 15 | 67 | 81 | 92 | 115 | 121 | 164 | 153 | 0 | 0 | 0 | 3 | 2 | 6 | 1 | 0 | 5 | 2 | 4 | 1 |
Nucleus | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 3 | 3 | 6 | 5 | 7 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 43 | 24 | 114 | 56 | 35 | 46 | 65 | 28 | 28 | 25 | 28 | 37 | 6 | 8 | 2 | 0 | 0 | 2 | 31 | 12 | 33 | 8 | 12 | 6 |
Nucleolus | 0 | 0 | 2 | 2 | 3 | 9 | 18 | 20 | 22 | 32 | 47 | 55 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 4 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 1 | 7 | 5 | 5 | 9 | 10 | 19 | 0 | 0 | 0 | 6 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 80 | 14 | 102 | 63 | 107 | 86 | 112 | 85 | 39 | 81 | 58 | 81 | 9 | 10 | 8 | 24 | 3 | 6 | 4 | 1 | 1 | 0 | 3 | 3 |
Unique Cell Count | 186 | 81 | 287 | 213 | 262 | 275 | 406 | 403 | 308 | 388 | 419 | 459 | 64 | 79 | 156 | 74 | 18 | 25 | 221 | 109 | 378 | 110 | 173 | 170 |
Labelled Cell Count | 237 | 105 | 389 | 264 | 321 | 345 | 520 | 493 | 394 | 482 | 553 | 602 | 79 | 95 | 183 | 97 | 19 | 34 | 221 | 109 | 378 | 110 | 173 | 170 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.1 | 13.5 | 12.0 | 11.6 | 10.9 | 9.3 | 9.7 | 9.7 | 9.1 | 8.8 | 8.9 | 9.1 | 11.9 | 11.5 | 12.0 | 11.7 | 15.1 | 16.4 | 12.2 | 13.1 | 14.1 |
Std Deviation (1e-4) | 2.1 | 2.0 | 1.9 | 2.1 | 1.8 | 1.6 | 1.6 | 1.7 | 1.7 | 1.7 | 1.8 | 1.7 | 1.8 | 1.9 | 1.4 | 2.4 | 2.9 | 2.6 | 2.2 | 2.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.14 | -0.37 | -0.31 | -0.3 | -0.39 | -0.44 | -0.44 | -0.39 | -0.01 | -0.06 | 0.01 | -0.04 | 0.34 | 0.46 | 0.02 | 0.12 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 2.5 | 2.8 | 2.9 | 2.5 | 2.8 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 4.0 | 3.1 | 3.7 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -1.1 | -0.4 | -1.7 | -1.7 | -1.2 | -1.1 | -1.1 | 0 | 0 | 0 | 4.5 | 5.1 | 0 | 0 |
Cytoplasm | 2.6 | 3.0 | 1.7 | 1.7 | 3.4 | 2.2 | 3.0 | 2.8 | 1.6 | 0 | 0 | 4.3 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.3 | 0.9 | -1.6 | 0 | -0.8 | -1.7 | -4.5 | -2.1 | -4.7 | 5.0 | 5.9 | 7.7 | -5.5 | -3.2 | -3.8 |
Endosome | 2.2 | 1.8 | 3.1 | 3.2 | 4.8 | 3.4 | 5.0 | 3.3 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.9 | -2.0 | -4.2 | -3.9 | -4.1 | -4.5 | -5.4 | -4.8 | -5.6 | 0 | 0 | -3.1 | 3.9 | 0 | 0 |
Mitochondria | 1.8 | 0.1 | 6.3 | 5.4 | 6.1 | 9.3 | 8.2 | 10.1 | 8.8 | 0 | 0 | -3.0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -3.1 | -6.9 | -6.0 | -7.0 | -10.5 | -8.8 | -10.6 | -10.8 | -10.5 | -4.6 | -4.9 | -8.8 | -6.6 | -3.4 | -3.1 |
Nucleolus | 0 | 0 | 2.2 | 2.9 | 3.1 | 4.0 | 4.4 | 5.4 | 5.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | 1.3 | -1.1 | -2.2 | -4.2 | -6.6 | -4.2 | -6.8 | -5.5 | -3.3 | -3.9 | -7.1 | -0.5 | -1.6 | -1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.8496 | 9.172 | 8.7303 | 8.6831 | 7.9633 | 9.018 | 11.8533 | 11.9969 | 11.3402 | 10.7714 | 11.6652 | 11.5715 | 11.1955 | 11.4196 | 11.4859 | 11.0696 | 11.312 | 11.1669 |
Actin | 0.0077 | 0.0022 | 0.0081 | 0.0054 | 0.0013 | 0.0038 | 0.0006 | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0051 | 0.0007 | 0.0005 | 0.0484 | 0.0031 | 0.0013 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 |
Bud Neck | 0.0006 | 0.0006 | 0.0004 | 0.0027 | 0.0021 | 0.0015 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0004 | 0.0003 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0003 | 0.0001 |
Bud Site | 0.0003 | 0.0038 | 0.0006 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0011 | 0.0001 | 0 |
Cell Periphery | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0028 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0008 | 0.0004 | 0.0011 | 0.0002 | 0.0002 |
Cytoplasm | 0.0124 | 0.0512 | 0.0271 | 0.0289 | 0.0103 | 0.0152 | 0.0016 | 0.0052 | 0.0013 | 0.0015 | 0.0009 | 0.0005 | 0.007 | 0.0033 | 0.0064 | 0.0034 | 0.0024 | 0.0008 |
Cytoplasmic Foci | 0.005 | 0.0068 | 0.0054 | 0.01 | 0.0044 | 0.0008 | 0.0012 | 0.0012 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0042 | 0.0008 | 0.0007 | 0.0069 | 0.0014 | 0.0003 |
Eisosomes | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.2762 | 0.5547 | 0.6361 | 0.6323 | 0.4806 | 0.4346 | 0.5776 | 0.7467 | 0.8426 | 0.948 | 0.7507 | 0.5778 | 0.4487 | 0.6868 | 0.8078 | 0.795 | 0.5816 | 0.5463 |
Endosome | 0.0248 | 0.0193 | 0.0177 | 0.0285 | 0.028 | 0.0061 | 0.008 | 0.0076 | 0.0037 | 0.0039 | 0.0044 | 0.002 | 0.0214 | 0.0075 | 0.0064 | 0.0123 | 0.0214 | 0.0062 |
Golgi | 0.0045 | 0.0026 | 0.0024 | 0.0092 | 0.0032 | 0.0013 | 0.0015 | 0.0014 | 0.0006 | 0.0002 | 0.0012 | 0.0006 | 0.0062 | 0.0027 | 0.0012 | 0.0071 | 0.0065 | 0.002 |
Lipid Particles | 0.0236 | 0.0191 | 0.0143 | 0.0096 | 0.0331 | 0.0089 | 0.0132 | 0.0268 | 0.0065 | 0.001 | 0.0057 | 0.0104 | 0.0128 | 0.046 | 0.0143 | 0.0085 | 0.0126 | 0.006 |
Mitochondria | 0.007 | 0.0006 | 0.0016 | 0.0028 | 0.011 | 0.0009 | 0.0016 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0011 | 0.0005 | 0.0002 | 0.0022 | 0.0021 | 0.0004 |
None | 0.0007 | 0.0005 | 0.0082 | 0.0002 | 0.0013 | 0.0003 | 0.004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.003 | 0.0002 | 0.0001 | 0.002 | 0.0004 | 0.0003 |
Nuclear Periphery | 0.5642 | 0.2725 | 0.2257 | 0.2401 | 0.3475 | 0.4566 | 0.3679 | 0.1944 | 0.1361 | 0.0326 | 0.2245 | 0.3966 | 0.4383 | 0.2356 | 0.1507 | 0.0942 | 0.323 | 0.4181 |
Nucleolus | 0.0043 | 0.0011 | 0.004 | 0.0001 | 0.0005 | 0.0023 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0.0003 | 0 |
Nucleus | 0.0388 | 0.0309 | 0.0234 | 0.0149 | 0.0586 | 0.0472 | 0.0042 | 0.0018 | 0.0009 | 0.0008 | 0.0043 | 0.003 | 0.0162 | 0.002 | 0.0025 | 0.0016 | 0.0244 | 0.0062 |
Peroxisomes | 0.0024 | 0.0002 | 0.0082 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0 | 0.0021 | 0.0001 | 0 |
Punctate Nuclear | 0.0062 | 0.0135 | 0.0043 | 0.001 | 0.0018 | 0.0055 | 0.002 | 0.0026 | 0.0001 | 0 | 0.0008 | 0.0003 | 0.017 | 0.0004 | 0.0005 | 0.0031 | 0.0052 | 0.0005 |
Vacuole | 0.0067 | 0.0139 | 0.006 | 0.0067 | 0.0077 | 0.0063 | 0.006 | 0.0038 | 0.0025 | 0.0094 | 0.0016 | 0.0015 | 0.005 | 0.0048 | 0.0034 | 0.0031 | 0.0047 | 0.0023 |
Vacuole Periphery | 0.0139 | 0.0055 | 0.0055 | 0.0052 | 0.0075 | 0.0074 | 0.0096 | 0.0043 | 0.0038 | 0.0014 | 0.0042 | 0.0063 | 0.0109 | 0.0079 | 0.0046 | 0.0051 | 0.0097 | 0.0086 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.9123 | 28.0676 | 29.2575 | 30.3572 | 30.0467 | 17.1845 | 28.0413 | 31.4673 | 27.7332 | 34.1005 |
Translational Efficiency | 0.5716 | 0.486 | 0.5156 | 0.4347 | 0.4538 | 0.9387 | 0.5078 | 0.4557 | 0.5464 | 0.4503 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
228 | 1086 | 543 | 1281 | 2296 | 1854 | 2306 | 1137 | 2524 | 2940 | 2849 | 2418 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1163.11 | 1165.04 | 1330.20 | 1233.89 | 1077.46 | 1153.67 | 1351.11 | 1301.44 | 1085.20 | 1157.87 | 1347.12 | 1265.65 |
Standard Deviation | 142.23 | 201.61 | 185.28 | 192.54 | 154.16 | 176.85 | 189.31 | 178.12 | 155.07 | 186.46 | 188.73 | 188.93 |
Intensity Change Log 2 | — | 0.002392 | 0.193656 | 0.085226 | — | 0.098596 | 0.326511 | 0.272474 | — | 0.049457 | 0.259072 | 0.178308 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002606 | 0.000424 | 0.001249 | 0.006841 | 0.001569 | 0.000664 | 0.001621 | 0.002584 | 0.001663 | 0.000575 | 0.001550 | 0.004839 |
Bud Neck | 0.002783 | 0.000385 | 0.003126 | 0.002263 | 0.000320 | 0.000307 | 0.000688 | 0.002041 | 0.000543 | 0.000336 | 0.001153 | 0.002158 |
Bud Site | 0.007349 | 0.000798 | 0.003699 | 0.013799 | 0.001377 | 0.000999 | 0.001419 | 0.005723 | 0.001917 | 0.000925 | 0.001853 | 0.010002 |
Cell Periphery | 0.000634 | 0.000380 | 0.000301 | 0.002562 | 0.000465 | 0.000389 | 0.000334 | 0.000764 | 0.000480 | 0.000386 | 0.000328 | 0.001716 |
Cytoplasm | 0.016321 | 0.005312 | 0.028570 | 0.015869 | 0.003627 | 0.014744 | 0.009489 | 0.005616 | 0.004773 | 0.011260 | 0.013125 | 0.011048 |
Cytoplasmic Foci | 0.014955 | 0.002241 | 0.010264 | 0.005407 | 0.001806 | 0.006977 | 0.001188 | 0.004201 | 0.002994 | 0.005228 | 0.002918 | 0.004840 |
Eisosomes | 0.000083 | 0.000019 | 0.000032 | 0.000209 | 0.000047 | 0.000027 | 0.000032 | 0.000065 | 0.000050 | 0.000024 | 0.000032 | 0.000142 |
Endoplasmic Reticulum | 0.598832* | 0.822427* | 0.250944* | 0.826479* | 0.848482* | 0.736820* | 0.806659* | 0.865899* | 0.825931* | 0.768442* | 0.700743* | 0.845015* |
Endosome | 0.041625 | 0.020382 | 0.017645 | 0.023723 | 0.019607 | 0.014745 | 0.014481 | 0.026965 | 0.021596 | 0.016827 | 0.015084 | 0.025248 |
Golgi | 0.010995 | 0.002009 | 0.000534 | 0.006867 | 0.002646 | 0.004253 | 0.000723 | 0.005849 | 0.003400 | 0.003424 | 0.000687 | 0.006388 |
Lipid Particles | 0.033276 | 0.011143 | 0.016950 | 0.034733 | 0.018472 | 0.018028 | 0.010950 | 0.028784 | 0.019809 | 0.015485 | 0.012094 | 0.031936 |
Mitochondria | 0.005031 | 0.001945 | 0.000939 | 0.000644 | 0.001381 | 0.003000 | 0.000662 | 0.000691 | 0.001711 | 0.002611 | 0.000715 | 0.000666 |
Mitotic Spindle | 0.012335 | 0.000528 | 0.006523 | 0.002838 | 0.000892 | 0.000978 | 0.001652 | 0.001449 | 0.001926 | 0.000812 | 0.002580 | 0.002185 |
None | 0.000053 | 0.001346 | 0.000382 | 0.001307 | 0.002121 | 0.001295 | 0.001135 | 0.000760 | 0.001934 | 0.001314 | 0.000992 | 0.001050 |
Nuclear Periphery | 0.072805 | 0.040290 | 0.316967* | 0.004471 | 0.028845 | 0.084609 | 0.062111 | 0.007368 | 0.032816 | 0.068238 | 0.110685* | 0.005833 |
Nuclear Periphery Foci | 0.139906 | 0.082857 | 0.289041* | 0.037224 | 0.065216 | 0.097067 | 0.079124 | 0.032299 | 0.071963 | 0.091818 | 0.119133 | 0.034908 |
Nucleolus | 0.000056 | 0.000039 | 0.000829 | 0.000019 | 0.000045 | 0.000311 | 0.000025 | 0.000009 | 0.000046 | 0.000211 | 0.000179 | 0.000014 |
Nucleus | 0.002668 | 0.000690 | 0.026092 | 0.001146 | 0.000445 | 0.005130 | 0.001593 | 0.000423 | 0.000645 | 0.003490 | 0.006263 | 0.000806 |
Peroxisomes | 0.001317 | 0.000132 | 0.000706 | 0.000462 | 0.000381 | 0.000908 | 0.000142 | 0.000126 | 0.000465 | 0.000621 | 0.000250 | 0.000304 |
Vacuole | 0.030819 | 0.005133 | 0.024398 | 0.011757 | 0.001495 | 0.006730 | 0.005655 | 0.007182 | 0.004144 | 0.006140 | 0.009228 | 0.009606 |
Vacuole Periphery | 0.005550 | 0.001517 | 0.000808 | 0.001378 | 0.000761 | 0.002020 | 0.000315 | 0.001201 | 0.001194 | 0.001834 | 0.000409 | 0.001295 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.51 | 0.83 | -2.11 | -4.74 | -3.75 | 1.56 | -0.18 | -1.15 | -2.47 | -1.04 | 2.06 | 0.05 | -3.40 | -5.27 | -3.62 |
Bud Neck | 2.91 | -0.59 | 0.47 | -7.17 | 1.69 | 0.13 | -3.53 | -2.51 | -2.53 | -1.94 | 1.97 | -4.32 | -4.57 | -5.27 | -2.69 |
Bud Site | 2.37 | 1.22 | -1.95 | -7.51 | -5.09 | 0.92 | -0.52 | -3.98 | -4.43 | -3.85 | 2.33 | -0.21 | -7.31 | -8.66 | -7.45 |
Cell Periphery | 0.82 | 1.00 | -3.29 | -4.44 | -4.57 | 1.02 | 2.02 | -3.01 | -3.50 | -4.20 | 1.46 | 2.36 | -4.65 | -5.02 | -5.20 |
Cytoplasm | 2.26 | -1.91 | 0.04 | -6.05 | 3.05 | -7.18 | -6.19 | -2.78 | 4.50 | 2.39 | -5.47 | -7.31 | -6.11 | -0.58 | 1.04 |
Cytoplasmic Foci | 3.45 | 1.05 | 2.55 | -3.07 | 2.39 | -4.75 | 0.95 | -2.41 | 1.88 | -3.11 | -2.65 | -0.11 | -2.32 | 0.36 | -2.44 |
Eisosomes | 1.92 | 1.50 | -2.27 | -4.54 | -4.20 | 1.15 | 0.77 | -1.08 | -3.32 | -2.54 | 1.70 | 1.06 | -3.44 | -5.16 | -4.66 |
Endoplasmic Reticulum | -8.79 | 12.48 | -8.62 | 0.25 | -39.32* | 12.34 | 5.68 | -1.28 | -11.57 | -5.82 | 7.41 | 14.82 | -1.70 | -8.93 | -16.07 |
Endosome | 3.64 | 4.03 | 3.00 | -1.87 | -2.96 | 3.67 | 3.82 | -3.59 | -6.16 | -6.27 | 3.66 | 4.93 | -2.49 | -6.38 | -7.65 |
Golgi | 1.68 | 1.96 | 0.74 | -3.34 | -4.55 | -1.62 | 3.88 | -2.58 | -1.13 | -4.34 | -0.04 | 4.14 | -2.71 | -2.75 | -6.16 |
Lipid Particles | 3.25 | 2.18 | -0.22 | -6.42 | -3.89 | 0.13 | 3.36 | -2.82 | -2.84 | -4.84 | 2.04 | 3.59 | -4.36 | -6.16 | -7.40 |
Mitochondria | 0.73 | 0.98 | 1.05 | 1.45 | 1.44 | -2.41 | 2.78 | 1.92 | 3.30 | -0.07 | -1.38 | 2.25 | 2.28 | 3.59 | 0.27 |
Mitotic Spindle | 2.21 | 0.96 | 1.71 | -3.04 | 1.89 | -0.15 | -1.44 | -1.20 | -1.17 | 0.14 | 1.73 | -1.11 | -0.68 | -2.93 | 0.50 |
None | -2.86 | -4.73 | -2.18 | 0.04 | -1.57 | 1.66 | 1.83 | 2.16 | 1.15 | 0.71 | 1.33 | 1.97 | 1.59 | 0.63 | -0.14 |
Nuclear Periphery | 3.02 | -14.51 | 6.66 | 12.06 | 23.58 | -13.15 | -10.25 | 9.89 | 18.84 | 17.90 | -10.91 | -18.21 | 13.68 | 22.33 | 26.81 |
Nuclear Periphery Foci | 3.91 | -8.33 | 7.16 | 7.84 | 21.50 | -6.23 | -3.14 | 7.96 | 13.24 | 11.03 | -4.51 | -9.71 | 9.69 | 14.99 | 19.49 |
Nucleolus | 1.26 | -2.89 | 2.35 | 2.52 | 3.03 | -2.91 | 1.38 | 2.30 | 3.26 | 1.72 | -2.81 | -2.59 | 2.46 | 3.36 | 3.14 |
Nucleus | 1.60 | -6.06 | 1.18 | -2.28 | 6.94 | -5.99 | -4.34 | -1.14 | 5.09 | 2.04 | -5.39 | -7.51 | -1.62 | 4.55 | 6.96 |
Peroxisomes | 1.25 | 0.61 | 0.92 | -2.99 | 1.23 | -2.18 | 3.56 | 4.00 | 3.15 | 0.44 | -0.93 | 1.74 | 1.36 | 1.96 | -0.49 |
Vacuole | 4.83 | 0.96 | 3.37 | -4.42 | 4.26 | -6.08 | -7.25 | -6.39 | -0.86 | -1.71 | -2.29 | -6.06 | -5.62 | -3.86 | -0.55 |
Vacuole Periphery | 1.27 | 1.50 | 1.30 | 0.34 | -1.88 | -3.38 | 3.81 | -1.05 | 1.49 | -2.16 | -1.69 | 2.58 | -0.30 | 1.52 | -3.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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