Standard name
Description Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0 0.14 0 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.15 0.2 0.06 0.24 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.08 0.09 0.06 0.06 0.1 0.07 0.09 0.08 0.06 0.11 0.1 0.14 0.2 0.22 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.49 0.72 0.37 0.38 0.41 0.31 0.37 0.34 0.31 0.22 0.3 0.22 0.72 0.75 0.76 0 0 0 0.79 0.81 0.84 0.82 0.79 0.88
Endosome 0 0 0 0.05 0 0.07 0.06 0.11 0.07 0.11 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.44 0.4 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.1 0.06 0.24 0.2 0.23 0.37 0.31 0.39 0.33 0 0 0 0 0.11 0.24 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.23 0.3 0.4 0.26 0.13 0.17 0.16 0.07 0.09 0.06 0.07 0.08 0.09 0.1 0 0 0 0.08 0.14 0.12 0.09 0.08 0.07 0
Nucleolus 0 0 0 0 0 0 0 0.05 0.07 0.08 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0.43 0.17 0.36 0.3 0.41 0.31 0.28 0.21 0.13 0.21 0.14 0.18 0.14 0.13 0.05 0.32 0.17 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 2 0 3 0 15 17 14 14 18 23 3 3 4 10 0 2 1 1 11 2 5 1
Bud 0 0 0 2 0 5 6 8 17 13 19 25 0 0 0 2 0 0 1 1 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 3 7 3 8 0 0 0 0 0 0
Cell Periphery 0 1 10 3 5 8 6 6 6 8 9 16 5 5 24 15 1 6 0 0 0 0 0 0
Cytoplasm 8 5 9 18 24 17 24 40 22 34 35 26 7 8 22 15 4 1 0 0 0 0 1 0
Endoplasmic Reticulum 91 58 107 82 107 85 151 139 95 84 124 99 46 59 118 3 0 0 173 88 317 89 136 148
Endosome 0 1 4 10 10 18 26 43 22 44 28 48 1 1 3 0 0 0 3 0 2 1 2 1
Golgi 8 0 21 7 9 1 6 5 1 0 3 1 2 1 1 17 8 10 0 0 4 2 1 0
Mitochondria 4 1 16 21 15 67 81 92 115 121 164 153 0 0 0 3 2 6 1 0 5 2 4 1
Nucleus 1 0 1 0 2 1 2 3 3 6 5 7 0 0 1 2 0 0 0 0 0 0 0 0
Nuclear Periphery 43 24 114 56 35 46 65 28 28 25 28 37 6 8 2 0 0 2 31 12 33 8 12 6
Nucleolus 0 0 2 2 3 9 18 20 22 32 47 55 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 1 1 2 4 2 4 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 1 7 5 5 9 10 19 0 0 0 6 1 0 1 0 0 0 1 0
Vac/Vac Membrane 80 14 102 63 107 86 112 85 39 81 58 81 9 10 8 24 3 6 4 1 1 0 3 3
Unique Cell Count 186 81 287 213 262 275 406 403 308 388 419 459 64 79 156 74 18 25 221 109 378 110 173 170
Labelled Cell Count 237 105 389 264 321 345 520 493 394 482 553 602 79 95 183 97 19 34 221 109 378 110 173 170


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 13.5 12.0 11.6 10.9 9.3 9.7 9.7 9.1 8.8 8.9 9.1 11.9 11.5 12.0 11.7 15.1 16.4 12.2 13.1 14.1
Std Deviation (1e-4) 2.1 2.0 1.9 2.1 1.8 1.6 1.6 1.7 1.7 1.7 1.8 1.7 1.8 1.9 1.4 2.4 2.9 2.6 2.2 2.3 1.8
Intensity Change (Log2) -0.04 -0.14 -0.37 -0.31 -0.3 -0.39 -0.44 -0.44 -0.39 -0.01 -0.06 0.01 -0.04 0.34 0.46 0.02 0.12 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.5 2.8 2.9 2.5 2.8 3.2 0 0 0 0 0 0
Bud 0 0 0 0 0 4.0 3.1 3.7 4.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -1.1 -0.4 -1.7 -1.7 -1.2 -1.1 -1.1 0 0 0 4.5 5.1 0 0
Cytoplasm 2.6 3.0 1.7 1.7 3.4 2.2 3.0 2.8 1.6 0 0 4.3 0 0 0
Endoplasmic Reticulum 0.3 0.9 -1.6 0 -0.8 -1.7 -4.5 -2.1 -4.7 5.0 5.9 7.7 -5.5 -3.2 -3.8
Endosome 2.2 1.8 3.1 3.2 4.8 3.4 5.0 3.3 4.7 0 0 0 0 0 0
Golgi -1.9 -2.0 -4.2 -3.9 -4.1 -4.5 -5.4 -4.8 -5.6 0 0 -3.1 3.9 0 0
Mitochondria 1.8 0.1 6.3 5.4 6.1 9.3 8.2 10.1 8.8 0 0 -3.0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.1 -6.9 -6.0 -7.0 -10.5 -8.8 -10.6 -10.8 -10.5 -4.6 -4.9 -8.8 -6.6 -3.4 -3.1
Nucleolus 0 0 2.2 2.9 3.1 4.0 4.4 5.4 5.6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Vacuole -1.4 1.3 -1.1 -2.2 -4.2 -6.6 -4.2 -6.8 -5.5 -3.3 -3.9 -7.1 -0.5 -1.6 -1.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ice2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available