Standard name
Human Ortholog
Description Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.05 0.07 0.09 0.16 0.21 0.23 0.26 0.28 0.24 0.29 0.05 0.11 0.16 0 0.1 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.09 0.16 0.05 0 0 0.06 0 0 0 0.11 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.11 0.09 0.09 0.06 0.31 0.55 0.54 0.6 0.6 0.73 0.57 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.62 0.47 0.82 0.59 0.7 0.55 0.37 0.34 0.28 0.25 0.26 0.26 0.73 0.52 0.41 0.47 0.65 0.6 0.43 0.54 0.49 0.43 0.26 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.23 0.36 0.09 0.21 0 0 0 0 0 0 0 0 0.17 0.27 0.31 0.24 0.25 0.08 0.28 0.22 0.12 0.25 0.21 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.23 0.09 0 0 0 0 0 0 0 0.08 0.27 0.26 0 0 0 0.06 0.05 0.1 0 0.14 0.14
Vac/Vac Membrane 0.06 0.11 0 0 0.09 0.09 0.06 0 0 0.05 0 0 0 0 0 0.24 0.1 0.15 0.06 0.08 0.14 0.15 0.19 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 1 0 0
Bud 0 1 0 1 3 1 6 12 9 15 14 14 0 0 0 0 0 0 3 0 0 2 5 5
Bud Neck 0 0 0 2 2 4 2 1 3 5 0 6 1 1 1 0 0 0 1 0 0 2 3 8
Bud Site 1 0 0 2 2 4 5 3 11 19 14 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 3 1 23 53 65 134 110 142 186 123 146 14 43 78 1 2 15 1 2 0 1 4 8
Endoplasmic Reticulum 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 1 2 0
Endosome 0 0 0 19 23 8 9 3 8 4 1 2 10 35 75 2 0 1 13 3 3 5 42 59
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 1 0 1
Mitochondria 48 5 2 29 35 126 346 260 321 391 367 280 3 2 1 0 1 5 6 0 0 1 6 13
Nucleus 114 21 18 190 399 222 232 161 153 166 132 130 188 203 195 18 13 55 94 44 38 73 100 62
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 2 0 0 0 0 0 0 1 0 1 1 2 4
Nucleolus 43 16 2 69 15 3 6 7 3 1 3 1 44 103 151 9 5 7 61 18 9 42 81 78
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 1 0 75 53 15 23 20 21 19 19 22 20 104 126 1 0 2 12 3 8 6 55 54
Vac/Vac Membrane 11 5 0 5 50 37 39 19 20 33 21 19 8 4 9 9 2 14 13 6 11 25 72 65
Unique Cell Count 184 45 22 321 572 402 634 480 539 655 505 495 257 388 480 38 20 92 219 82 78 170 385 379
Labelled Cell Count 226 52 23 416 635 487 802 597 692 842 695 634 289 498 636 42 24 101 219 82 78 170 385 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.3 5.4 6.0 5.6 4.4 3.9 4.2 3.8 3.8 3.5 3.8 6.9 6.8 6.4 8.4 7.4 8.5 6.2 6.5 6.5
Std Deviation (1e-4) 0.9 0.9 1.4 1.3 1.2 1.3 1.1 1.4 1.7 2.0 1.7 1.4 1.0 1.2 1.3 1.6 1.3 1.6 1.7 1.2 1.1
Intensity Change (Log2) – – – 0.15 0.05 -0.3 -0.46 -0.38 -0.52 -0.5 -0.62 -0.53 0.34 0.32 0.24 0.63 0.44 0.64 0.2 0.26 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 2.3 2.5 2.1 2.5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -2.1 -1.2 -2.5 -4.3 -4.6 -5.3 -5.9 -5.7 -5.6 -0.9 -2.7 -3.8 -2.6 -1.2 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1.8 2.2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2.8 2.8 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3511 3.7704 3.4862 3.1919 2.9642 3.4558 1.6834 2.5934 2.1995 1.4428 2.1314 2.0553 1.6292 2.2805 1.5905 1.9247 1.3813 1.3155
Actin 0.0032 0 0.0008 0.0002 0.0004 0.001 0.009 0.0001 0.007 0.0361 0.0022 0.0002 0.0218 0 0.0009 0.0007 0.0014 0.0003
Bud 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0003 0.0005 0.0001 0.0001 0.0009 0.0008 0.0003 0.0008 0.0005 0.0002
Bud Neck 0.0023 0.0013 0.0023 0.0017 0.0043 0.0046 0.003 0.0015 0.0018 0.0159 0.0007 0.0044 0.0031 0.0028 0.0022 0.0089 0.0025 0.0077
Bud Periphery 0.0004 0.0002 0.0004 0.0002 0.0004 0.0003 0.0007 0.0005 0.0007 0.0018 0.0002 0.0004 0.0015 0.0012 0.0006 0.0012 0.0014 0.0007
Bud Site 0.0016 0.0023 0.0015 0.0007 0.001 0.0005 0.0017 0.0023 0.002 0.0018 0.0012 0.0007 0.0117 0.0022 0.0047 0.0218 0.0023 0.0011
Cell Periphery 0.0005 0.0002 0.0003 0.0001 0.0001 0.0002 0.0003 0.0003 0.0003 0.0005 0.0001 0.0002 0.0006 0.0008 0.0006 0.001 0.0004 0.0005
Cytoplasm 0.0022 0.0006 0.0007 0.0008 0.0013 0.0004 0.0008 0.0032 0.0011 0.0002 0.0004 0.0018 0.0093 0.001 0.0017 0.0006 0.0006 0.0005
Cytoplasmic Foci 0.0068 0.0006 0.0018 0.0004 0.0012 0.0003 0.013 0.0035 0.0043 0.0066 0.0136 0.0003 0.0136 0.0004 0.0044 0.0086 0.0078 0.0009
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0015 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0013 0.0004 0.0001 0.0001 0.0005 0.0003 0.0004 0.0004 0.0007 0.0001 0.0007 0.0025 0.0017 0.0002 0.0003 0.0002 0.0002 0.0001
Endosome 0.0074 0.0023 0.0038 0.0005 0.0047 0.0018 0.0097 0.0033 0.0091 0.0048 0.006 0.0048 0.0213 0.0009 0.0093 0.003 0.0423 0.003
Golgi 0.0017 0.0002 0.0012 0.0001 0.0005 0.0004 0.0035 0.0003 0.0018 0.0089 0.0053 0.0003 0.0099 0.0001 0.0034 0.0021 0.011 0.0022
Lipid Particles 0.0106 0.0054 0.0034 0.0005 0.0027 0.0009 0.0168 0.0039 0.0071 0.0682 0.036 0.0006 0.015 0.0019 0.0069 0.0049 0.022 0.0007
Mitochondria 0.0046 0.0002 0.0005 0.0003 0.0006 0.0016 0.0025 0.0004 0.0059 0.0033 0.0022 0.0004 0.0224 0.0003 0.0098 0.0012 0.0775 0.0058
None 0.0032 0.0003 0.0003 0.0005 0.0006 0.0008 0.0005 0.0008 0.0017 0.0002 0.0003 0.001 0.0101 0.0001 0.0008 0.0002 0.0003 0.0001
Nuclear Periphery 0.0113 0.0055 0.0032 0.0041 0.036 0.0037 0.0033 0.0079 0.006 0.0008 0.0809 0.0039 0.0078 0.0039 0.0047 0.0024 0.014 0.0081
Nucleolus 0.4339 0.5232 0.615 0.5444 0.3407 0.4925 0.36 0.3307 0.4901 0.3552 0.1908 0.3836 0.3492 0.3783 0.5556 0.4143 0.1537 0.4106
Nucleus 0.4534 0.4366 0.3471 0.399 0.543 0.4664 0.5418 0.6172 0.4423 0.4755 0.6302 0.5849 0.4498 0.587 0.3714 0.5114 0.6426 0.5388
Peroxisomes 0.0099 0.0001 0.0005 0.0001 0.0005 0.0002 0.0065 0.0004 0.0014 0.0131 0.0227 0.0001 0.0172 0.0001 0.0065 0.0047 0.0026 0.0005
Punctate Nuclear 0.0424 0.015 0.0134 0.0456 0.0586 0.022 0.0197 0.0171 0.0114 0.0041 0.0043 0.0077 0.0176 0.0034 0.0073 0.0023 0.0083 0.014
Vacuole 0.0021 0.0049 0.003 0.0005 0.0019 0.0015 0.0055 0.0048 0.0035 0.0019 0.0007 0.0019 0.0103 0.0118 0.0072 0.0085 0.0052 0.0035
Vacuole Periphery 0.001 0.0005 0.0003 0.0001 0.0008 0.0004 0.0008 0.0011 0.0014 0.0004 0.0013 0.0003 0.0037 0.0027 0.0012 0.0012 0.0032 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.9428 22.7695 25.006 20.9155 26.4194 20.3655 19.1085 15.2277 14.7619 23.075
Translational Efficiency 1.8091 1.7065 1.2098 1.1519 1.336 1.3158 1.2697 1.2102 1.4135 1.3211

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome
Localization
Cell Percentages nucleus (59%), nucleolus (14%), mixed (24%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Air1

Air1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Air1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available