Standard name
Description Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.05 0.07 0.09 0.16 0.21 0.23 0.26 0.28 0.24 0.29 0.05 0.11 0.16 0 0.1 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.09 0.16 0.05 0 0 0.06 0 0 0 0.11 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.11 0.09 0.09 0.06 0.31 0.55 0.54 0.6 0.6 0.73 0.57 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.62 0.47 0.82 0.59 0.7 0.55 0.37 0.34 0.28 0.25 0.26 0.26 0.73 0.52 0.41 0.47 0.65 0.6 0.43 0.54 0.49 0.43 0.26 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.23 0.36 0.09 0.21 0 0 0 0 0 0 0 0 0.17 0.27 0.31 0.24 0.25 0.08 0.28 0.22 0.12 0.25 0.21 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.23 0.09 0 0 0 0 0 0 0 0.08 0.27 0.26 0 0 0 0.06 0.05 0.1 0 0.14 0.14
Vac/Vac Membrane 0.06 0.11 0 0 0.09 0.09 0.06 0 0 0.05 0 0 0 0 0 0.24 0.1 0.15 0.06 0.08 0.14 0.15 0.19 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 1 0 0
Bud 0 1 0 1 3 1 6 12 9 15 14 14 0 0 0 0 0 0 3 0 0 2 5 5
Bud Neck 0 0 0 2 2 4 2 1 3 5 0 6 1 1 1 0 0 0 1 0 0 2 3 8
Bud Site 1 0 0 2 2 4 5 3 11 19 14 10 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 3 1 23 53 65 134 110 142 186 123 146 14 43 78 1 2 15 1 2 0 1 4 8
Endoplasmic Reticulum 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 1 2 0
Endosome 0 0 0 19 23 8 9 3 8 4 1 2 10 35 75 2 0 1 13 3 3 5 42 59
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 1 0 1
Mitochondria 48 5 2 29 35 126 346 260 321 391 367 280 3 2 1 0 1 5 6 0 0 1 6 13
Nucleus 114 21 18 190 399 222 232 161 153 166 132 130 188 203 195 18 13 55 94 44 38 73 100 62
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 2 0 0 0 0 0 0 1 0 1 1 2 4
Nucleolus 43 16 2 69 15 3 6 7 3 1 3 1 44 103 151 9 5 7 61 18 9 42 81 78
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 1 0 75 53 15 23 20 21 19 19 22 20 104 126 1 0 2 12 3 8 6 55 54
Vac/Vac Membrane 11 5 0 5 50 37 39 19 20 33 21 19 8 4 9 9 2 14 13 6 11 25 72 65
Unique Cell Count 184 45 22 321 572 402 634 480 539 655 505 495 257 388 480 38 20 92 219 82 78 170 385 379
Labelled Cell Count 226 52 23 416 635 487 802 597 692 842 695 634 289 498 636 42 24 101 219 82 78 170 385 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.3 5.4 6.0 5.6 4.4 3.9 4.2 3.8 3.8 3.5 3.8 6.9 6.8 6.4 8.4 7.4 8.5 6.2 6.5 6.5
Std Deviation (1e-4) 0.9 0.9 1.4 1.3 1.2 1.3 1.1 1.4 1.7 2.0 1.7 1.4 1.0 1.2 1.3 1.6 1.3 1.6 1.7 1.2 1.1
Intensity Change (Log2) 0.15 0.05 -0.3 -0.46 -0.38 -0.52 -0.5 -0.62 -0.53 0.34 0.32 0.24 0.63 0.44 0.64 0.2 0.26 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 2.3 2.5 2.1 2.5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -2.1 -1.2 -2.5 -4.3 -4.6 -5.3 -5.9 -5.7 -5.6 -0.9 -2.7 -3.8 -2.6 -1.2 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1.8 2.2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2.8 2.8 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Air1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available